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LEAD (Pb) CONTENT AND STOMACH HISTOLOGICAL STRUCTURE IN TILAPIA (Oreochromis mossambicus) FISH ORGANS LIVED IN THE WATERS OF UPAL PT. ITDC shanon lestari; Ngurah Intan Wiratmini; Job Nico Subagio
SIMBIOSIS Vol 8 No 2 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Nusa Dua merupakan kawasan pariwisata dengan rancang bangun yang komprehensif dan terpadu sebagai sarana akomodasi wisata berkelas Internasional. Pengelolaan limbah cair dikelola secara terpadu pada Unit Pengelolaan Air Limbah (UPAL) milik PT. ITDC. Hotel – hotel membuang limbah cairnya melalui collection pit yang akan diakumulasikan di kolam UPAL. Tiga kolam terakhir digunakan sebagai kolam pemeliharaan ikan salah satunya ikan mujair, sebagai bioindikator. Ikan mujair dikolam itu dapat hidup namun belum diketahui bagaimana histologi dan anatomi organnya, sehingga perlu kajian histologis. Rancangan penelitian yang digunakan Rancangan Acak Lengkap dengan pengambilan sampel ikan mujair pada dua titik pengambilan dari area lagoon. Sampel ikan mujair diambil sebanyak 8 kali sebagai ulangan, dari setiap kolam yaitu kolam nomor 2 B dan kolam nomor 3. Variabel yang diamati adalah kandungan timbal (Pb) pada daging ikan dan kerusakan jaringan pada organ lambung . Rerata kandungan timbal dalam daging ikan dari kolam 2B sebesar 1,85 mg/kg, sedangkan dari kolam 3 sebesar 1,24 mg/kg. Jumlah sel dengan inti karyoreksis dan jaringan yang mengalami desquamasi sel epitel pada lambung ikan mujair (O. mossambicus) dari kolam 3 lebih rendah secara nyata dibandingkan di kolam 2B. Peralihan dari kolam 2B ke kolam 3 mengurangi kandungan timbal dan kerusakan histologis. Kata Kunci: mujair, lambung, logam berat, Pb

VARIATION MORPHOMETRY AND MORPHOLOGICAL CHARACTER OF GOLDEN APPLE SNAIL (Pomacea canaliculata) IN RICE FIELD ABIANSEMAL VILLAGE, BADUNG I Gede Agus Suarmustika; Ni Made Suartini; Job Nico Subagio
SIMBIOSIS Vol 6 No 2 (2018)
Publisher : Jurusan Biologi Universitas Udayana

The purpose of this study was to determine variations in morphology and morphological characteristics of golden apple snails (P. canaliculata) in rice fields in Abiansemal Village. Gold apple snails can be found living in fresh water, including lakes, rivers, small ditches and also in rice fields. Rice field habitats in Bali can still be found in Abiansemal village, Badung regency, but not much information has been disclosed about the snails in the rice field habitat, especially the golden apple snail species (P. canaliculata). Therefore, it is necessary to do research on the golden apple snails in Abiansemal Village. This research was carried out in rice fields located in Abiansemal Village, Abiansemal District, Badung Regency, Bali Province. The study was conducted from April to May 2017. Sampling of golden apple snail (P. canaliculata) was carried out in three plots of rice fields in three banjars in Abiansemal Village, namely in the Latu Sari, Banjar Aseman, and Juwet banjars. The rice field was chosen randomly and then in each selected field of rice the golden apple snail sample was taken using a 1x1m square. Samples on each plot of rice were taken three times. All samples of golden snail contained in the squared were taken and cleaned and then preserved with 70% alcohol. The golden apple snail sample obtained was calculated for its density and all morphological and morphometric characters were analyzed descriptively. In this study it was found that the morphology of golden apple snail patterned lines for morphometric characters, the shell height was higher in the banjar Juwet area, for the width of the shell mouth was greater in the banjar area Aseman and the height of the shell cover was higher in the banjar area of Latu Sari, while the morphology was plain patterned for morphometric characters, the height of the shell and the height of tendrils were higher in the banjar area of Latu Sari, and for the height and width of the cover the shell was higher and wider in the banjar area of Aseman, while for the other morphometric characters the results were found almost the same. Keywords: snail, rice field, morphology, morphometry

MYOGLOBIN CONTENT IN CARDIAC MUSCLE OF PHYSICAL EXERCISE INDUCED RAT (Rattus norvegicus) Sri Rahayu; Rini Puspitaningrum; Mohammad Sadikin
SIMBIOSIS Vol 4 No 2 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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ABSTRACT The research was aimed to analyzing the impact of 7 days arobic exercise in elevating rat cardiac muscle myoglobin. Experimental in vivo study on 20 male Wistar rat induced by 20m/ min treadmill aerobic exercise for 1, 3 and 7 days. Research was done in Animal house and protein laboratory, Biochemistry and Molecular Biology Department, FK UI and laboratory of Biochemistry and Molecular Biology, Biology Department FMIPA UNJ for 10 months. Myoglobin concentration was analyzed by spectrofotometry techique based on myoglobin Soret absorbance in ?503nm. Myoglobin protein expression was assesed with Western blot technique. Standard curve measurement on myoglobin content with horse myoglobin shown that there was an increase on the absorbance value. The result showedanthat the standar curve was able to be used as standard curve to calculate myoglobin concentration on cardiac muscle within 503 nm. Spectrophotometry myoglobin concentration measurement on Soret absorbance has shown increasing concentration on myoglobin since the first treatment day. Mean myoglobin concentration value has increased on the 3rd and 7th day, with highest value on the 7th treatment day (0,0656 ± 0,0001). Western blot analysis showed ng on darker band on treatment group compared on to control. When aerobic exercise was given longer, the band shown darker means that myglobin content was higher. Myoglobin concentration can be measured by spectrophotometry on 503 nm. Aerobic physical exercise increased myoglobin on rat cardiac muscle since the first treatment day and still increase oduring the treatment day. Highest level was shown on the 7th treatment day. Keywords : myoglobin, cardiac, muscle, exercise, rat

INVENTARISASI JENIS-JENIS TANAMAN HIAS INTRODUKSI DI DESA PENGLIPURAN, KABUPATEN BANGLI, BALI Ayu Zuraida; A.A. Gde Raka Dalem; Martin Joni
SIMBIOSIS Vol 6 No 1 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Panglipuran Village is one of the traditional villages in Bali that still prioritizes harmonization and sense of togetherness. The village is located in Kelurahan Kubu, Kecamatan Bangli, Bangli District, Bali. Panglipuran Village has many types of plants. The varieties of plants can be influenced by several factors: one of them is introduction. This research is conducted to examine the introduction of decorative plants in Panglipuran Village, with the purpose of providing information to villagers about those plants, so that their potential can be utilized in other travel destinations, especially the ones in Bali. The method used in this research is purposive sampling. Data were obtained through direct field observation and interviews with local villagers, and later were identified at Laboratorium Taksonomi Tumbuhan FMIPA, Udayana University. According to the results of this research, 32 types of introduction plants which comes from 24 different famili was found. Introduction of decorative plants in Panglipuran Village can be classified into 3 groups, which are 22 types of decorative flower plants, 8 types of decorative leaf plants, and a single type of decorative stem plants. Keywords: inventorisation, Panglipuran Village, decorative plants, introduction

ANALISA KERAGAMAN GENETIK KELAPA RANGDA (Cocos nucifera L.) DI BALI BERDASARKAN PENANDA DNA MIKROSATELIT Ni Luh Putu Rika Sugiantari; I Ketut Junitha; Eniek Kriswiyanti
SIMBIOSIS Vol 3 No 2 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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The research aimed to analyze genetic variation of Rangda coconut in Bali based on microsatellite DNA markers. The young leaf (busung) were collected from Gunaksa village at Klungkung regency, Tulikup village at Gianyar regency, Kubu village at Bangli regency, Sanghyang village, Jembrana regency one sample each, and three samples are from Ngis village at Karangasem regency. Doyle and Doyle DNA extraction method was used to extract DNA from the samples. Three primer pairs of CnCirC3, CNZ05 and CNZ51 were used to amplify DNA samples. Amplicons were electrophoresed on PAGE 10% and stained with silver nitrate. The allele size was determined by plotting the amplicon migration distance on semilog paper. The results showed there were 11 alleles with an average of 13.33 alleles per primer. There were 3 alleles on primer CNZ05, 4 alleles on primer CnCirC3 and CNZ51 each. Heterozigosity expectation (He) value on primer CnCirC3, CNZ05, and CNZ51 were 0,49±0,04, 0,27±0,03 and 0,71±0,06 consecutively. The result showed that Genetic relationship among 7 coconut samples were divided into two big groups. Key word : Rangda coconut, Microsatellite marker, Number allele, Genetic relationship

EKSTRAKSI DNA DARI SPERMA PADA KONDOM DAN KAIN YANG TERSIMPAN SAMPAI DUA BELAS HARI Vandus Jehuda
SIMBIOSIS Vol I, No 1, Tahun 2013
Publisher : Jurusan Biologi Universitas Udayana

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Sperm is a biological material that is often used as evidence in rape cases. The research of DNA extraction from sperm was conducted in order to determine DNA whether it could be extracted from sperm in the condom and fabrics that were stored for 3, 6, 9, and 12 days and to know the success of its amplification. The sample is dripped into a condom and fabrics each 150 ?L, then stored for 3, 6, 9, and 12 days. DNA extraction was performed using phenol-chloroform method that had been modified and DNA amplification using PCR Mastermix. The results showed that DNA could be extracted and the extraction could be amplified from sperm in the condom and fabrics that were stored for 3, 6, 9 and 12 days.

INVESTIGATING THE GLOBAL WARMING/CLIMATE CHANGE (LA-NINA) PHENOMENA, LAND USE CHANGE AND ENVIRONMENTAL DEGRADATION MITIGATION ON BALI ISLAND I Wayan Kasa; Ida Bagus Wayan Gunam
SIMBIOSIS Vol 7 No 2 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Up to now Bali island still suffering from light to heavy rainfall (August 2016), eventhough it must be hot season as usual (April to October). Such situation is recognised as La-Nina phenomenon. Methode empolyed in this study is collecting primary (direct field check) and scondary data (literature study). Results showed that, the La-Nina phenomena most affected coffee plantation farmers in the village of Sepang, district of Busungbiu, Buleleng regency. The coffee fruit fall out from stem branch prior to harvest time and becoming black in colour. In addition, some coffee fruit skin are white in colour in comparison to red to black during normal hot season. Land use change could also be seen over Bali. Increase sea level on some beaches are also another disaster around Bali. Such accidence was significantly happened on the beach of Lepang village, district of Banjarangkan, Klungkung regency. It was found that such increases puddling area of more or less five hectare of paddy rice field on that area. The global warming/climate change phenomenon also cause habitat loss of specific ancient crab who live in Nusa Penida beach and move into the Giri Putri holy cave to survive. However, the only government do to compromise and mitigate such abnormal environmental degradation would be by strengthening the essen of the Eka Karya botanical garden of Bedugul as an open green field area for refreshing.

DAYA HAMBAT INFUSA RIMPANG KUNYIT (Curcuma longa Linn) TERHADAP PERTUMBUHAN Escherichia coli dan Vibrio sp. pada IKAN KERAPU LUMPUR (Epinephelus tauvina) di PASAR KEDONGANAN KABUPATEN BADUNG, BALI Ni Putu Sinta Puspa Dewi; I. B. Gede Darmayasa; Ni Wayan Sudatri
SIMBIOSIS Vol 5 No 2 (2017)
Publisher : Jurusan Biologi Universitas Udayana

ABSTRACTFish is perishable food because it contains high enough protein and water, therefore to easy damaged by microorganism. Then the control of mircoorganism should be done to maintaining the quality of fish to stay awake.Turmeric is one of the herbs that has compound content curcumin and fenolik being able to control the growth of contaminants microorganism. The objective of this research is to find out the power of immersion of mudfish grouper (Epinephelus tauvina) by infusa turmeric rhizome (Curcuma longaLinn) through sampled from Kedonganan Market, Bali. The treatment given to mudfish grouper was turmeric rhizome infusion withconcentration of 0%, 5%, 10%, 15% and 20% tested in vitro and in vivo on the growth of E. coli and Vibrio sp. The resourcesinhibited turmeric infusa rhizome is determined by counting the population of bacteria test after treatment by the method of dilution sampling (Plating Method). The results showed that turmeric rhizome infusion was able significantly (P<0,05) inhibitionto the growth of E. coli and Vibrio sp. both in vitro and in vivo. The control (0%) in vitro population E. coli and Vibrio sp. each of 5,23x102 CFU/g and 4,98x102 CFU/g higher than with the treatment of concentration 5%, 10%, 15% and 20%. Population E. coli and Vibrio sp. in testing by in vivo (concentration 0%) each is obtained 4,17x102 CFU/g dan 4,20x102 CFU/g in statistic is different (P<0,05) with the concentration 10%, 15% and 20%. Keywords: Epinephelus tauvina, Curcuma longa Linn, E. coli, Vibrio sp.

STRUKTUR HATI MENCIT (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK DAUN KALIANDRA MERAH (Calliandra calothyrsus Meissn.) Haryo Seto Wicaksono; Inna Narayani; Iriani Setyawati
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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This research aims to determine the effect red calliandra leaf extract on weight, anatomy andhistology of male mice liver. Treatment was administered orally with varying doses (2, 4, 6mg/ kg). Control grups was given 0,9% NaCl. This study used a completely randomizeddesign (CRD), consisting of 12 groups with three replications. Histological observationsshowed that red calliandra leaf extract significantly affected the damage of male mice liver.Damage level of nucleus pyknotic and fatty degeneration increased in line with the increasingdoses in treatment for 7 and 21 days, as well as the dilation of central venous in treatment for7 days. The damage increase in line with the leght of treatment in the fatty degeneration (dose2 mg/kg bw), nuclues pyknotic (doses 2 and 6 mg/kg bw), infiltration of inflammatory cells(dose 4 mg/kg bw), sinusoid congestion (doses 2, 4 and 6 mg/kg bw), and central venousdilatation (dose 2 and 4 mg/ kg bw).Keywords : Red calliandra, male mice, liver histology

ISOLASI DAN IDENTIFIKASI BAKTERI PELARUT FOSFAT POTENSIAL PADA TANAH KONVENSIONAL DAN TANAH ORGANIK Ilham .; Ida Bagus Gede Darmayasa; Retno Kawuri
SIMBIOSIS Vol 2 No 1 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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Phosphate solubilizing bacteria are decomposer bacteria functioning as major role in soilenrichment. That is because it is able to perform phosphate dissolution mechanism by excreting lowparticles weight of organic acids. The bacteria utilize simple carbon compounds (rootexudatesofplantsandthe restof theplant).Phosphate solubilizing bacteria activity will be high at atemperature of 300C - 400C. This study aims to isolate and identify thephosphate solubilizingbacteria intheconventional soil and organic soil originated from Japan. Conventional and organic soil sample aresent from Collage Of Agricultural Ibaraki University in Japan. The Isolation of pathogenic bacteria isperformed by the dilution method (platting method). Results of isolation of bacteria lisolates obtained 2on ground phosphate conventional solubilizing (TKO1 andTKO2) and 4 isolates of bacteria onsoilorganicsolubilizingphosphate(TOR1, TOR2, TOR3andTOR4). Bacteria lisolates TKO2 highestphosphate solubilizing. Based on the identificati on results using the Microgen ™ Kit GNA + B - IDSystem and Determine Bergeys 's Manual of Biology (Garrity et al., 2006)TKO2 isolate is identified asYersinia sp.Keywords : Phosphate solubilizing bacteria, conventional soil, organic soil, Yersinia sp.

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Kota denpasar,

Bali

INDONESIA