cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota surakarta,
Jawa tengah
INDONESIA
Nexus Biomedika
Published by Universitas Sebelas Maret
ISSN : -     EISSN : -     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 292 Documents
 15   16   17   18   19 
The Effects of Propolis Ethanol Extract on The Decreasing Number of The Mouses Spermatids Induced by Cigarettes Smoke Mugi Tri Sutikno; . Muthmainah; Riza Novierta Pesik
Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background:The propolis ethanol extract contains flavonoid, terpenoid, Caffeic Acid Phenethyl Ester (CAPE), vitamin A, vitamin C and vitamin E which are potential for being antioxidant to protect the spermatids from the danger of free radical. The aims of this research were to know the effects of propolis ethanol extract on the decreasing number of the mouses spermatids induced by cigarettes smoke and to know the effect of dosage raising level of the propolis ethanol extract towards the research subject. Methods:This research was a laboratory experiment using the posttest only controlled group design. The sampels were 25 Swiss Webster mice (age around 2-3 months and approximately 20 grams of weight). They were classified into 5 groups which consisted of 5 mice in each group. KK group was without any intervention. KI group was only induced by the cigarettes smoke. KP1, KP2 and KP3, all were induced by the cigarettes smoke and in a row, got 5.6 mg (dosage level I), 11.2 mg (dosage level II) and 16.8 mg (dosage level III) of propolis ethanol extract. These interventions were done for two weeks. The decreasing number of spermatids was counted through the inspection under microscope and counting of five chosen rounded-looks seminiferous tubules. The data were analyzed by One-Way ANOVA test and Post Hoc Multiple Comparisons test. Results:The result of One-way ANOVA p = 0.000 (p < ?) showed differences between each research groups. The Post Hoc test of the spermatids number showed significant differences between KK-KI, KK-KP1, KK-KP2, KK-KP3, KI-KP1, KI-KP2, KP1-KP2, KP1-KP3, KP2 -KP3 groups but KI and KP3 wasnt. Conclusions:The propolis ethanolic extract can inhibit the decreasing number of the mouses spermatids which is induced by cigarettes smoke and the raising dosage from 5.6 mg to 11.2 mg is the succeeded dosage to inhibit the decreasing number of the spermatids. Keywords: propolis ethanol extract, spermatid, mouse, cigarettes smoke.
Effects of Delayed Urinalysis on Urine Leukocytes Counts Sheila Savitri; Dian Ariningrum; Bhisma Murti
Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Abstract

Introduction: Urinalysis is an effective and comprehensive urine examination method. Delayed urine examination may affect the urinalysis results, which in turn may have consequences on a patients diagnosis and treatment. This study focuses on the effects of delayed urinalysis on the urine leukocyte counts in patients with urinary tract infection. Methods: This study used a cross sectional design. The research took place at the Clinical Pathology Laboratory of RSUD Dr. Moewardi Hospital in Surakarta, in November 2015. The consecutive sampling method was used to collect 30 urine samples as for analysis. The dependent variable of this study was the urine leukocyte count at 0, 120 and 180 minutes, while the independent variable was the gap time of urinalysis. Urine sample collected from the respondents was divided into three different containers with the same amount of urine and the container was marked (0, 120, 180). The urinalysis was performed according to the order of the mark on each container. This study used Sysmex UX 2000 to count the amount of urine leukocyte. The data, then, were analyzed by the Friedman test. Result: The result showed that there was no significant difference in urine leukocytes counts in delayed urinalysis, shown by the statistical calculations using the Friedman test which obtained a p value of 0.122. Urine samples were collected from 17 female respondents (57%) and 13 male respondents (43%). The age of the respondents ranged from 1 to 78 years old. The average pH of the urine samples was 6.2 with an average specific gravity of 1.012. Conclusions: There is no significant difference in urine leukocyte counts in delayed urinalysis up to 180 minute. Keywords: Delayed Urinalysis, Urine Leukocytes, Sysmex UX 2000
Efek Antihelmintik Ekstrak Akar Ceguk (Quisqualis indica, L.) pada Ascaris suum, Goeze in Vitro Antonius Bagus Budi Kurnia; Sri Haryati; Bambang Sukilarso Sakiman
Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Radix-Quisqualis indica L. contains saponins and potassium quisqualata that are known having anthelmintic effect. This study aimed to determine the effect of extract of radix-Quisqualis indica L. toward mortality of Ascaris suum, Goeze in vitro Methods: This study was a laboratory experimental research using the post-test only controlled group design. Subjects were adult Ascaris suum, Goeze. The sampling technique used was purposive sampling. Subjects were arranged into 7 groups, each group consisted of 4 worms, replications were performed 4 times. Saline solution was used in negative control group and pyrantel pamoate 5 mg/ml was used in positive control group, while the treatment group was treated with extract of radix-Quisqualis indica, L. at concentration of 50%, 60%, 70%, 80%, and 90%. Subjects were immersed in 25 ml of test solution and incubated at 37C. Observations were done every 1 hour and the numbers of dead worms were counted. Data were analyzed with linier regression and probit analysis. Results: The result of observation showed that mean time of total death of Ascaris suum, Goeze in the negative control and in the positive control were 96.00 hours and 1.25 hours, each. While mean time of total death of Ascaris suum, Goeze in the extract of radix-Quisqualis indica, L. at concentration of 50%, 60%, 70%, 80%, 90% were 11.50 hours, 9.50 hours, 8.00 hours, 5.75 hours and 3.50 hours, consecutively. Shapiro Wilk test showed that the distribution of the data was normal (p > 0.05). Linear regression test showed that the variation of the extract concentration affected time of worm death. Probit analysis showed that LC50 and LT50 of the extract of radix-Quisqualis indica L. were 69.80% and 3.90 hours. Conclusions: The extract of radix-Quisqualis indica, L. affected mortality of Ascaris suum, Goeze in vitro. Keywords: extract of radix Quisqualis indica, L., Ascaris suum, Goeze,
Uji Ekstrak Daun Kumis Kucing Dalam Menurunkan Kadar Alanin Transaminase pada Mencit yang Diinduksi Minyak Goreng Pemanasan Berulang Steffi Meidiana; Diding Heri Prasetyo; . sarsono
Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Backgrounds: Orthosiphon stamineus Benth leaves contain various antioxidants like flavonoids, organic acids, and terpenoids. The main active chemical substances which are derived from flavonoids, namely sinensetin, and non-flavonoids, namely rosmarinic acids, have antioxidants activities as free radical scavengers. This study aimed to determine the effects of Orthosiphon stamineus Benth leaves extract in decreasing the levels of ALT in male strain Swiss webster mice induced by reheated cooking oil. Methods: This study was an experimental laboratory with a post test only controlled group design. The samples were 25 male strain Swiss webster mice, 2-3 months old and + 25-30 grams weight, each. Samples were taken incidentally sampling, arranged into 5 groups randomly, consisted of 5 mice each group. The control group (K) was administered distilled water and treatment group 1 (P 1) were administered reheated cooking oil. While treatment group 2 (P 2), treatment group 3 (P 3) and treatment group 4 (P 4) were consecutively administered 3.5 mg, 7.0 mg and 14.0 mg/25 gr BW of Orthosiphon stamineus Benth leaves extract and reheated cooking oil for 14 days. On the 15th day, blood samples were drawn from the mice through plexus retroorbitalis using microtubuler tube then their ALT levels were measured. Data were analyzed using One-way ANOVA (? = 0.05) and followed by Post Hoc Multiple Comparisons test (LSD) (? = 0.05). Results: Mean rate of alanin transaminase level in group K, P 1, P 2, P 3 and P 4 were 54.600 + 7.765 U/L; 95.000 + 23.537 U/L; 62.200 + 7.463 U/L; 60.400 + 6.427 U/L and 57.400 + 10.922 U/L, in a row. Conclusions: Orthosiphon stamineus Benth leaves extract decreased alanin transaminase level in mice induced reheated cooking oil. Keywords: ALT, Orthosiphon stamineus Benth leaves extract, reheated cooking oil.
Pengaruh Ekstrak Etanol Daun Kenikir (Cosmos caudatus Kunth.) terhadap Kerusakan Struktur Histologis Ginjal Mencit (Mus musculus L.) yang Diinduksi Parasetamol Lina Kristanti Wibowo; . Suyatmi; Yulia Sari
Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Kenikir leaves contain various kind of antioxidants, such as vitamin C, ?-carotene, minerals, and polyphenols which are known to protect kidneys from oxidative stress caused by free radical exposure. The purpose of this study is to evaluate the nephroprotective effect of Kenikir leaf extract toward histological damage of the mice's kidney cells induced by Parasetamol. Methods: This was a laboratory experimental study with the posttest only controlled group design. The samples were 35-Swiss webster male mice, aged 2-3 months, and weight about 20 gram. Samples were divided into five groups randomly. Normal group (KN) was given distilled water and Negative control (KK(-)) was given Parasetamol. Extract dosage of 2.8 mg, 5.6 mg, and 11.2 mg of kenikir leaves were given daily for 14 days to treatment group 1 (KP1), treatmen group 2 (KP2), and treatment group 3 (KP3), respectively. A toxic dosage of Parasetamol as much as 5.07 mg, was given to negative control group and treatment groups on day 12th, 13th, and 14th. On day 15th , mice were sacrificed by neck dislocation. The kidneys were taken for histological preparation and stained with HE. The histological damage was assessed based on quantifying the pyknosis, karyorrhexis, and karyolysis on 50 proximal tubuli of epithelial cells. The data were analyzed using One-way ANOVA test (? = 0.05) and Post Hoc Multiple Comparisons test (? = 0.05). Results: The most damaged cells were found in KK(-) and the least in KN. The significant difference was observed among groups (One-way ANOVA test p = 0.000). The significant difference was observed between groups (Post Hoc test p = 0.000). They were KN-KK(-), KN-KP1, KN-KP2, KN-KP3, KK(-)-KP1, KK(-)-KP2, KK(-)-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusions: Ethanol extract of Kenikir leaves can prevent the histological damage of the mice's kidney cell induced by Parasetamol. The increasing dose of the extract is followed by the increase of protective effect. Keywords: Kenikir leaves, histological damage, mice's kidney, Parasetamol
Antifungal Effect of Ascaris suum, Goeze Pseudocoelom and Perienteric Body Fluid to Candida albicans, (C.P. Robin) Berkhout In Vitro Zakka Zayd Zhullatullah Jayadisastra; Yulia Sari; Sri Haryati
Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.864 KB)

Abstract

Introduction: Ascaris suum, Goeze pseudocoelom and perienteric body fluid is suggested to have antifungal effect to Candida albicans, (C.P. Robin) Berkhout because of Ascaris suum antibacterial factor (ASABF) which presents in pseudocoelom body fluid and neuropeptide-like protein 29 and caenacin-2 which present in perienteric body fluid. This research is conducted to prove the antifungal effect of Ascaris suum, Goeze pseudocoelom and perienteric body fluid to Candida albicans, (C.P. Robin) Berkhout in vitro. Methods: This was a quasi-experimental research. Candida albicans, (C.P. Robin) Berkhout speciments were tested using Ascaris suum, Goeze pseudocoelom and perienteric body fluid with concentration of each were 80%, 90%, and 100%. Specimens were incubated in temperature of 37oC for 24 hours and the inhibitory zone was measured. Result: Ascaris suum, Goeze pseudocoelom and perienteric body fluid didnt create any inhibitory zone in Candida albicans, (C.P. Robin) Berkhout inoculation. Conclusion: Ascaris suum, Goeze pseudocoelom and perienteric body fluid didn't have antifungal effect to Candida albicans, (C.P. Robin) Berkhout. Keywords: Candida albicans, Ascaris suum, Pseudocoelom Body Fluid, Perienteric Body Fluid.
Pengaruh Ekstrak Daun Salam (Syzygium polyanthum Wight) Terhadap Waktu Kematian Ascaris suum,Goeze In Vitro Gagat Ragil Andaru P; Bambang Sukilarso Sakiman; Sri Haryati
Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Syzygium polyanthum Wight leaves contain tannin that has been known to have anthelmintic effect. This study aimed to determine the effect of Syzygium polyanthum Wight leaves extract toward death time of Ascaris suum, Goeze In Vitro Methods: The study was a laboratory experimental research using the post-test only controlled group design. Subjects were adult Ascaris suum, Goeze. The sampling technique used was purposive sampling. Subjects were divided into 7 groups, each group consisted of 4 worms, replication was performed 4 times. Saline solution was used in negative control group, pirantel pamoate 5 mg/ml was used in positive control group, while the treatment group used Syzygium polyanthum Wight leaves extract concentration of 70%, 75%, 80%, 85%, and 90%. Worms were immersed in 25 mL of the test solution and incubated at 37C. Observations were done every 1 hour and the number of dead worms was counted. Data were analyzed with regression linier and probit analysis. Results: Observations of total deaths mean time Ascaris suum, Goeze sp on negative control was for 96 hours, on the positive control was for 2 hours 30 minutes, and on the concentration of 70%, 75%, 80%, 85%, and 90% of Syzygium polyanthum Wight leaves extract, each was for 10 hours 15 minutes, 8 hours, 6 hours, 4 hours 45 minutes and 3 hours, consecutively. Linear regression test results showed that the variation of the concentration affected long worm death. Probit analysis results showed that LC50 and LT50 of Syzygium polyanthum Wight leaves extract, each was 76.228% and 3 hours 36 minutes. Conclusions:Based on this study, it could be concluded that of Syzygium polyanthum Wight leaves extract affected mortality of Ascaris suum, Goeze sp in vitro. The increasing of extract concentration was inversely proportional with the death time of worms Keywords: Syzygium polyanthum Wight leaves extract, Ascaris suum,Goeze.
Pengaruh Pemberian Ekstrak Daun Sambiloto Terhadap Kadar Kolesterol LDL Darah Tikus Putih (Rattus norvegicus) Yohana Trissya Anggraeni; Jarot Subandono; . Kustiwinarni
Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Andrographis paniculata,Nes leaves contain flavonoids, beta-sitosterol, and andrographolide which can lower LDL cholesterol level of blood. The objective of this research was to determine the effect of Andrographis paniculata,Ness leaves extract against blood LDL cholesterol level of white rats (Rattus norvegicus). Methods: This study was a laboratory experimental research design, using posttest only controlled group design. Subjects of this research were white rats (Rattus norvegicus), strain Wistar, male, 3 months old, 200 grams weight. By incidental sampling technique, data were collected from 25 rats. Those rats were arranged into 5 groups, each group consisted of 5 rats. The normal control group (KKn) was administered distilled water, while the negative control group (KKn(-)) and the 1st (KP1), 2nd (KP2) and 3rd treatment group (KP3) were administered high cholesterol diet (6.25 g/kg BW/day of egg yolk). Besides that, the KP1, KP2 and KP3 were treated with 160 mg, 320 mg, and 640 mg per 200 g BW of Andrographis paniculata,Ness leaves extract, successively. This research was held for 28 days. Data of blood LDL cholesterol levels were collected 10-12 hours after the last treatment. Later, the data was analyzed by One-Way ANOVA test (? = 0.05) and Posthoc Multiple Comparisons (LSD) test (? = 0.05). Results: The one-way ANOVA test showed a significant difference among five groups p = 0.000 (p <0.05). Whereas, the LSD test showed significant differences between KKn-KKn(-), KKn-KP1, KKn-KP2, KKn-KP3, KKn(-)-KP1, KKn(-)-KP2, KKn(-)-KP3, (p < 0.05) and non-significant differences between KP1-KP2, KP1-KP3, and KP2-KP3 (p > 0.05). Conclusions Andrographis paniculata,Ness leaves extract significantly lowered blood LDL cholesterol levels of Rattus norvegicus but the increase of the dose did not enhance its function in lowering blood LDL cholesterol. Keywords: Andrographis paniculata Ness leaves extract, LDL cholesterol
CCR2-V64IPolymorphism in Multidrug-Resistant TuberculosisPatients in Dr. Moewardi General Hospital in Surakarta Windhy Monica; Afiono Agung Prasetyo; Marwoto .
Nexus Biomedika Vol 4, No 3 (2015): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.862 KB)

Abstract

Background: Genetic variants of CCR2 (CC chemokine receptor type 2) are associated with susceptibility to tuberculosis (TB), however its association with Multidrug-Resistant Tuberculosis (MDR-TB) is still unknown. The aim of the present study was to find out the status of CCR2-V64I polymorphisms among MDR-TB patients in Dr. Moewardi General Hospital in Surakarta. Methods: Blood samples from 36 MDR-TB patients in Dr. Moewardi General Hospital in Surakarta were subjected to DNA isolation. The DNA was genotyped for CCR2-V64I polymorphisms by a polymerase chain reaction with sequence-specific primers (PCR-SSP). Results: The wild type (GG) was found in 21/36 (58%) samples. The heterozygous genotype (GA), homozygous genotype (AA), and A allele frequencies in the population respectively were 39%, 3%, and 22%. Conclusion: The frequency of mutant type (GA and AA) was found lower than the wild type (GG) in the population of MDR-TB patients. Keywords: CCR2-V64I, MDR-TB, Surakarta
Pengaruh Ekstrak Buah Jambu Biji Merah terhadap Kerusakan Struktur Histologis Hepar Mencit yang Diinduksi Asetaminofen Vasa Adi Wisnu Wardana; Suyatmi .; Bambang Sukilarso Sakiman
Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika
Publisher : Nexus Biomedika

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (13.86 KB)

Abstract

Background: Antioxidants in guava fruit are vitamin C, flavanoid, and lycopene which are estimated having hepato-protective function. The objectives of this research are to know the effect of guava fruit extract and in various doses on mice liver damage induced by acetaminophen. Methods: Samples of 28 male mice, Swiss webster type, 2-3 months old age and + 20gr of each weight, were divided into 4 groups of 7 mice. Guava fruit extract was given for 14 days in a row, while acetaminophen in toxic dose was given on the 12th, 13th, and 14th day. The score of hepatocyte damage was determined by pyknosis, karyorhexis, and karyolysis. The data was analized by One-Way ANOVA test (?= 0,05) and continued by Post Hoc Multiple Comparisons using Least Significant Differences(LSD) test(?= 0,05). Results:The group with the highest average damage of hepatocyte is KP1(84,233,207), and the groups with decrease average damage of hepatocyte are KP2 (48,762,964) and KP3(35,232,755). The results of One-Way ANOVA test showed a significant difference in all group (p<0.001). The statistical data result showed a significant difference of hepatic damage between K-P1, K-KP2, K-KP3, KP1-KP2, KP1-KP3, and KP2-KP3. Conclusion: Guava fruit extract can decrease the histological damage of the hepatocyte of mice induced by acetaminophen. The higher dose of guava fruit extract, the higher protection effect againts liver histological damage from acetaminophen toxic on mice. Key words: guava fruit extract, acetaminophen, histological damage, hepatocyte.

Page 17 of 30 | Total Record : 292

 15   16   17   18   19 

Filter by Year

2012 2017


Reset
Filter By Issues
All Issue Vol 6, No 2 (2017): Nexus Biomedika Vol 6, No 2 (2017): Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika Vol 6, No 1 (2017): Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika Vol 5, No 2 (2016): Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika Vol 5, No 1 (2016): Nexus Biomedika Vol 4, No 3 (2015): Nexus Biomedika Vol 4, No 3 (2015): Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika Vol 4, No 2 (2015): Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika Vol 4, No 1 (2015): Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika Vol 3, No 3 (2014): Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika Vol 3, No 2 (2014): Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika Vol 3, No 1 (2014): Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika Vol 2, No 3 (2013): Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika Vol 2, No 2 (2013): Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika Vol 2, No 1 (2013): Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika Vol 1, No 2 (2012): Nexus Biomedika Vol 1, No 1 (2012): Nexus Biomedika Vol 1, No 1 (2012): Nexus Biomedika More Issue