cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota semarang,
Jawa tengah
INDONESIA
Journal of Biomedicine and Translational Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 25032178     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Journal of Biomedicine and Translational Research (JBTR) is an open access, international peer-reviewed journal that considers articles on: clinical medicine, molecular medicine, tropical medicine, infectious diseases, cardiovascular medicine, molecular biology, genetics, immunology, microbiology, biochemistry, and pharmacotherapy with particular interest on the link between clinical and basic research called translational research.
Arjuna Subject : -
Articles 8 Documents
 1 
Search results for , issue "Vol 10, No 2 (2024): August 2024" : 8 Documents clear
The Effect of Sambiloto (Andrographis paniculata) Leaf Extract in Combination with Phosphomycin on The Germ Number, Urine Leukocyte Esterase, and Urine Procalcitonin Levels in Wistar Rats (Rattus norvegicus) Urinary Tract Infection Model Hadinata, Sebastian; Lestari, Endang Sri; Nugroho, Eriawan Agung; Nindita, Yora; Addin, Sofyan Rais
Journal of Biomedicine and Translational Research Vol 10, No 2 (2024): August 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i2.21966

Abstract

Background: Bacterial resistance to antibiotics is still common due to unwise use. Urinary Tract Infections (UTI), 80% ofwhich are caused by E. coli and other bacteria such as Enterobactersp, Klebsiellasp, S. aureus. Phosphomycin is a first-line antibiotic for UTI. Combining natural compounds with antibiotics is one treatment strategy to increase the effectiveness of anti-bacterialt herapy. Andrographispaniculata has been reported to have strong anti-infectiveactivity. This study aimed to prove the differences in the germ number, leukocyte esterase levels,and urine procalcitonin levels in Rattus norvegicus UTI model given the phosphomycin, Sambilotoleaf extract, and Sambiloto leaf extract-phosphomycin combination. Methods: Thirty Rattus novergicus rats were divided into five groups. All groups were induced 50 µl of E. coli bacterial inoculum for 7 days, followed by standard feed (negative control), fosfomycin (Monuril®) 54 mg (positive control), Sambilotoleaf extract (S1 [100 mg/BW], S2 [200 mg/BW],Sambiloto leaf extract-phosphomycin combination (FS1 [sambiloto 100 mg/BW and fosfomycin 54 mg], and FS2 [sambiloto200 mg/BW and phosphomycin 54 mg]) for the next 7 days orally.The germ number, leukocyte esterase, and urine procalcitonin were measured after all rats were given treatment.  Results: The largest averagere duction in the germ number, levels of leukocyte esterase, and urinary procalcitonin (4.80 ± 3.70 CFU/ml [p<0.05], 3,00 ± 6,71 cells/µL [p<0.05], 4,66 ± 1,35 ng/L [p<0.05] respectively)was observed in the combination of 200 mg/BW Sambiloto leaf extract-phosphomycin combination group.  Conclusion: A combination of Sambiloto leaf extract and phosphomycin reduced germ number, levels of leukocyte esterase and urinary procalcitonin in rat model of UTI. 
The Effect of Administration of Sapodilla Leaf Extract Cream (Manilkara Zapota (L.) P. Royen) On the Expression of PDGF And Il-10 Maharani, Sahara; Sumarawati, Titiek; Hermansyah, Dedi
Journal of Biomedicine and Translational Research Vol 10, No 2 (2024): August 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i2.22207

Abstract

Background: Sunburn is an acute inflammatory skin condition caused by exposure to UV rays. Excessive exposure increases the production of  ROS which, if accumulated, can lyse growth factors, one of which is PDGF and also form IL-10 immune suppression. This condition can be influenced by providing antioxidants and anti-inflammatories such as those contained in sapodilla leaf extract (Manilkara zapota (L.) P. Royen) which has many benefits such as anti-inflammatory, anti-pyretic, anti-tumor, antioxidant, anti-microbial, anti- diabetic, anti-lipid and anti-aging. In previous research, the polyphenol content in Sapodilla leaves was a potential source of inhibiting ROS. However, until now the role of Sapodilla leaf extract on UVB burns has not been studied.Objective: The aim of this research is to determine the effect of administering Sapodilla leaf extract cream on PDFG and IL-10 in Wistar rats that experienced burns due to exposure to UVB.Method: Experimental research with a post test only control group design approach. This research used 24 Wistar rats exposed to UVB rays which were divided into 4 groups (normal control, control with cream, 25% Sapodilla leaf extract cream, and 50% Sapodilla leaf extract cream). The ELISA (Enzyme-Linked Immunosorbent Assay) method was used to analyze PDGF and IL-10 levels in skin tissue.Results: The highest ratio of PDGF levels was found in (K3) 2.915 ± 0.368. The results of the one way Anova analysis had a p value of 0.024 (p<0.05) which stated that there were significant differences between treatment groups. In IL-10 levels there was an increase in K3 255.9 ± 35,563. In IL-10, the results of one way Anova analysis had a p value of 0.240 (p>0.05), which stated that there were no significant differences between treatment groups.Conclusion: Administration of sapodilla leaf extract cream at a dose of 25% had a significant effect on increasing PDGF levels and a slight increase in IL-10 in mice that experienced burns due to exposure to UVB.
Revealing the Potency of Camelia sinensis and Serenoa repens as Purinoreceptor Inhibitor for Benign Prostatic Hyperplasia Treatment through in Silico Study Putra, Mega Memory Rahasa; Seputra, Kurnia Penta
Journal of Biomedicine and Translational Research Vol 10, No 2 (2024): August 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i2.21211

Abstract

Background: Benign prostatic hyperplasia (BPH) is the most common prostate disease in elderly men that leads to a significant deterioration in patients' quality of life (QoL). Pharmacological therapy of 5-alpha reductase inhibitor and alpha adrenoreceptors blocker often causes several side effects that decrease the QoL, so it is necessary to develop a new treatment for BPH. Purinoreceptor is a novel receptor that can inhibit electrically evoked nerve-mediated contractions in the prostate. Tea leaves (Camellia sinensis) and Saw palmetto (Serenoa repens) are herbs that have potential as alternative therapies for BPHObjective: to reveal the mechanism of Camellia sinensis and Serenoa repens through purinoreceptor and the other receptorsMethods: Structures of active compounds were extracted from PubChem and protein from PBD. The active compounds Camellia sinensis and Serenoa repens to the target protein purinoreceptors, 5-alpha-reductase, and alpha adrenoreceptors was evaluated in silico using a docking server with Finasteride dan Tamsolusin as a control. Molecular docking method using dockingserver application.Results: Epigallocatechin gallate only compound that has potency in blocking purinoceptors and 5-alpha-reductase. Capric acid, Caprylic acid, Lauric acid, Linoleic acid, and Myristic acid have the potential to bind to alpha adrenoreceptor ligands.Conclusion: Camellia sinensis have potential and effects as alternative therapies in benign prostatic hyperplasia on the target protein purinoreceptors, 5-alpha-reductase, and alpha adrenoreceptors. But, Serenoa repens have potential only through alpha adrenoreceptors.
Antibiotic-Resistant Phenotype and Genotype of S. suis serotype 2 (SS2) Isolated from Humans in Bali, Indonesia MAYURA, I PUTU BAYU -; TARINI, NI MADE ADI; Susilawathi, Ni Made; YULIANDARI, PUTU
Journal of Biomedicine and Translational Research Vol 10, No 2 (2024): August 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i2.21985

Abstract

Background: In recent times, meningitis, an infection primarily attributed to the zoonotic bacteria Streptococcus suis, has emerged as a significant public health concern in Bali, Indonesia. Their resistance to a multitude of antibiotics has emerged as a contemporary threat, as opposed to their virulence. There is a current lack of reported information regarding the genetic or phenotypic susceptibility pattern of S. suis to antibiotics in Bali.Objective: The objective of this research endeavor was to ascertain the antibiotic susceptibility pattern of S. suis isolates in Bali, either through phenotypic or genetic means.Methods: The Clinical Microbiology Laboratory of Prof. I.G.N.G. Ngoerah Hospital was consulted for the acquisition of clinical specimens. Since April 2016 until April 2022, the antibiotic sensitivity of isolates was identified and assessed using the VITEK 2 Compact (Biomeriuex®). Determining the serotype and antibiotic resistance genes genetically required PCR employing multiple primers.Results: Successful isolation of sixty-six S. suis isolates occurred primarily from cerebrospinal fluid. The results demonstrated that all isolates exhibited phenotypic resistance to tetracycline, with one isolate demonstrating co-resistance to tetracycline and erythromycin/clindamycin. It is additionally corroborated genetically through the amplification of the tetM gene in every isolate, including those that exhibited concurrent resistance to erythromycin and tetracycline. The intTn gene, which is a member of the conjugate transposon Tn916 family, was amplified and utilized for horizontal media gene transfer on S. suis plasmids carrying the resistance genes ermB and tetM.Conclusion: This research represents the initial investigation into the antibiotic resistance phenotype and genotype of S. suis serotype 2 (SS2) isolated from human subjects in Bali, Indonesia. The findings suggest that the pathogenicity and widespread dissemination of SS2 in the community may have been influenced by the extensive use of tetracycline and the horizontal acquisition of the genetic element Tn916 with tetM.
Sorghum Tempeh on Cholesterol Levels and Histopathology of Aorta in High-Fat Diet-Induced Rat Model Bintang, Francisca Natalia; Lestari, Endang Sri; Afifah, Diana Nur; Muniroh, Muflihatul; Anjani, Gemala; Syauqy, Ahmad; Djamiatun, Kis
Journal of Biomedicine and Translational Research Vol 10, No 2 (2024): August 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i2.22689

Abstract

Background: Elevated cholesterol levels are associated with hypercholesterolemia, the primary cause of death and lost productivity, and a significant risk factor for the onset of cardiovascular disease. Sorghum is known for its high bioactive components and phenolic compounds, flavonoids, β-glucans, and dietary fiber, which act as anti-cholesterol properties.Objective: This study aimed to analyze the impact of sorghum tempeh on cholesterol levels and histopathology of aorta in rats fed a high-fat diet.Methods: A total of 24 male 8-weeks-old Sprague Dawley rats were randomly assigned to four groups: standard diet group (SD), high-fat diet control group (FD), rats fed high-fat diet + low dose of sorghum tempeh (T1), and high-fat diet + high dose of sorghum tempeh (T2). Measurements of cholesterol levels were determined using the total cholesterol ELISA method. Histopathology of aorta analysis was carried out after four weeks of intervention of the four treatment groups using Hematoxylin-Eosin staining.Results: The average total cholesterol levels post-intervention in the SD, FD, T1, and T2 were 89.986±2.089, 220.365±3.847, 121.161±4.111, and 97.836±2.504 mg/dL, respectively. The results showed that the total cholesterol level significantly decreased (p<0.05) after giving a formula of sorghum tempeh with doses of 0.75 g and 1.50 g per 200 g body weight of rats for four continuous weeks. Histopathology of the aorta in the FD and T2 groups showed a significant difference compared to the SD group. The result which was closest to the SD group was the T1 group.Conclusion: Sorghum tempeh is a high-fiber and antioxidant source that can control hypercholesterolemia by lowering serum total cholesterol. It is also possible to improve histopathology but not yet able to approach normal conditions. The administration of sorghum tempeh with a low dose is sufficient, and further research is still required to determine the effect of sorghum tempeh on aorta histopathology.
Psychotherapy Supportive Practice For Borderline Personality Disorder (A Clinical Module Development) Wardani, SpKJ, Natalia Dewi; Widiastuti, M I; Sudiyanto, Aris -; Hardian, Hardian -; Lukman, Petrin Redayani; Septiawan, Debree -; Primada, Lyla Fitrania; Uminah, Uminah -; Rasyad, Ula Faza Nayli
Journal of Biomedicine and Translational Research Vol 10, No 2 (2024): August 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i2.19628

Abstract

ABSTRACTBackground: Handling patients with Borderline Personality Disorder (BPD) who are hypersensitive to rejection, have unstable interpersonal relationships, self-image, affect, and behavior is a challenge for a psychiatrist. Supportive psychotherapy is one of the most widely mastered psychotherapy modalities in psychiatric education in Indonesia and is most widely practiced in psychiatric services.Objective: It is hoped that the development of a supportive psychotherapy clinical practice module for Borderline Personality Disorder will be helpful for psychiatrists in the handling of Borderline Personality Disorder patient.Methods: This research was conducted in three stages. Stage 1 was preparing the supportive psychotherapy module for 13 weeks. Stage 2 was for the module validation using face validity and content validity by two psychotherapy consultants. Stage 3 was the trials of the developed modules, where 2 interrater therapists applied the modules to treat 5 patients.Result: The face validity of the two experts for the supportive psychotherapy module for Borderline Personality Disorder in Indonesian language was 3.269, meaning that it mostly was done correctly. The results of the content validity of the two experts for the supportive psychotherapy module for Borderline Personality Disorder in Indonesian language was 81.165. The results of the two experts' face validity and content validity scores inferred that the supportive psychotherapy module for Borderline Personality Disorder in Indonesian language was suitable for use in services.Conclusion: Supportive psychotherapy module in Indonesian language for BPD is acceptable to be applied to treat Borderline Personality Disorder patients.
The Relationship Between the Duration of Kangaroo Mother Care and Edinburgh Postnatal Depression Scale Outcomes in Mothers with Preterm Infants Pranoto, Ivena Celia Eileen; Suswihardhyono, Adhie Nur Radityo; Wardani, Natalia Dewi
Journal of Biomedicine and Translational Research Vol 10, No 2 (2024): August 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i2.20443

Abstract

Background: Preterm birth has a negative impact on the health of the baby and increases the risk of postpartum depression in mothers. Kangaroo mother care (KMC) is a preterm baby care which is considered to increase bonding between mother and baby, thereby reducing the incidence of postpartum depression, which can be evaluated using the Edinburgh Postnatal Depression Scale (EPDS) instrument.Objective: To find out the relationship between the duration of KMC and EPDS outcomes in mothers with preterm infants.Methods: This study used a quasi-experimental method with non-randomized control group pre-test and post-test design and was conducted on 34 mothers with preterm infants who gave birth at Dr. Kariadi Hospital Semarang. The research subjects were selected using consecutive sampling method and were asked to perform kangaroo mother care for 60 minutes daily (control group) and 120 minutes daily (treatment group) for 14 days. Evaluation was carried out using the Edinburgh Postnatal Depression Scale questionnaire, which was completed twice, as a pre-test and post-test. Data analysis was performed using paired sample T-test and independent samples T-test to determine the relationship between variables.Results: Results showed that there was a significant relationship between the duration of KMC and EPDS outcomes. The difference between the decreased of EPDS scores in the control and treatment groups was significant (p=0.017). The significant decrease of EPDS score was found in the treatment group (p<0.001). The decrease of EPDS score in the control group was not significant (p=0.704).Conclusion: Increasing duration of KMC lowers the score of EPDS in mothers with preterm infants.
Neutrophil–Lymphocyte and Platelet–Lymphocyte Ratios are Predictors of Lung Malignancy Sutanto, Yusup S.; Rudiannor, Muhammad; Aphridasari, Jatu; Kurniawan, Hendra
Journal of Biomedicine and Translational Research Vol 10, No 2 (2024): August 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i2.20330

Abstract

Background: Inflammatory cells play an essential role in the neoplastic process by stimulating cancer proliferation, survival, and migration. Neutrophil-lymphocyte and platelet-lymphocyte levels can be used as the inflammatory tissue damage markers in cancer patients.Objective: This study aimed to analyze the increase of neutrophil-lymphocyte ratio (NLR) and platelet–lymphocyte ratio (PLR) as the predictive factors for lung malignancy.Methods: This study was a diagnostic cross-sectional study design in lung tumor patients at Dr. Moewardi Hospital Surakarta from August to October 2018. The subjects (60) were selected with consecutive sampling who take lung cancer diagnostic tests and divided into two groups of patients with lung tumors (30) and healthy (30) as control. The diagnostic procedures and neutrophil–lymphocyte and platelet–lymphocyte ratios calculation were performed on both groups. The optimum cutoff values for the NLR and PLR were calculated from the receiver operating curve analysis.Results: The statistical test found a significant difference in the neutrophil–lymphocyte and platelet–lymphocyte ratios between cancer patients and control (p = 0.0000). The lung cancer group exhibited an increase in the NLR with 90.0% sensitivity and 96.7% specificity with a cutoff of 2.71. The platelet–lymphocyte ratio had a cutoff of 136.63 at 83.3% sensitivity and 80.0% specificity.Conclusion: The increase of neutrophil-lymphocyte and the platelet-lymphocyte ratio can be used as a predictive predictor of lung malignancy.

Page 1 of 1 | Total Record : 8

 1 

Filter by Year

2024 2024


Reset
Filter By Issues
All Issue Vol 11, No 3 (2025): December 2025 Vol 11, No 2 (2025): August 2025 Vol 11, No 1 (2025): April 2025 Vol 10, No 3 (2024): December 2024 Vol 10, No 2 (2024): August 2024 Vol 10, No 1 (2024): April 2024 Vol 9, No 3 (2023): December 2023 Vol 9, No 2 (2023): August 2023 Vol 9, No 1 (2023): April 2023 Vol 8, No 3 (2022): December 2022 Vol 8, No 2 (2022): August 2022 Vol 8, No 1 (2022): April 2022 Vol 7, No 3 (2021): December 2021 Vol 7, No 2 (2021): August 2021 Vol 7, No 1 (2021): April 2021 Vol 6, No 3 (2020): December2020 Vol 6, No 2 (2020): Augusts 2020 Vol 6, No 1 (2020): April 2020 Vol 5, No 2 (2019): December 2019 Vol 5, No 1 (2019): July 2019 Vol 4, No 2 (2018): December 2018 Vol 4, No 1 (2018): July 2018 Vol 3, No 2 (2017): December 2017 Vol 3, No 1 (2017): July 2017 Vol 2, No 2 (2016): December 2016 Vol 2, No 1 (2016): July 2016 Vol 1, No 2 (2015): December 2015 Vol 1, No 1 (2015): April 2015 More Issue