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Journal of Biomedicine and Translational Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 25032178     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Journal of Biomedicine and Translational Research (JBTR) is an open access, international peer-reviewed journal that considers articles on: clinical medicine, molecular medicine, tropical medicine, infectious diseases, cardiovascular medicine, molecular biology, genetics, immunology, microbiology, biochemistry, and pharmacotherapy with particular interest on the link between clinical and basic research called translational research.
Arjuna Subject : -
Articles 173 Documents
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Recalcitrant Incomplete Secukinumab Administration in a Psoriasis Patient Gani Panjaitan, Joice Sonya; Suhartomi, Suhartomi
Journal of Biomedicine and Translational Research Vol 9, No 3 (2023): December 2023
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v9i3.19100

Abstract

Background: Psoriasis is an immunologic-mediated disease affected by genetic factors that may affect the skin, joints, and cardiovascular system. Some biological agents have been developed and approved by FDA to treat psoriasis. One of these biological agents is Secukinumab, a fully human IgG1κ anti-interleukin-17A(IL-17A) monoclonal antibody. Case Presentation: A seventeen female teenager came to Dermatovenerology Clinic with scaly patches in the forehead and hairline around ten months ago with a history of repeat Corticoticosteroid, DMARDs, and biologic agent treatment. Dermatology examination showed erythema, induration, and desquamation in head and extremities with PASI score of 1.2. She was treated with initial and maintenance doses of Secukinumab Injection.Conclusion: It can be concluded that the recalcitrant administration of Secukinumab in Psoriasis patients may decrease the treatment response.
The Impact of Butterfly Pea Flower (Clitoria ternatea L.) Extract on Atherosclerosis Biomarker Profiles in Obese White Rats (Rattus norvegicus L.) Harefa, Karnirius; Ritonga, Ahmad Hafizullah; Aritonang, Barita; Gurusinga, Rahmad; Wulan, Sri; Irmayani, Irmayani
Journal of Biomedicine and Translational Research Vol 10, No 1 (2024): April 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i1.20281

Abstract

 Background: Clitoria ternatea L., commonly known as butterfly pea flower, has gained attention as a potential agent in the prevention and treatment of atherosclerosis due to its rich content of bioactive compounds, such as anthocyanin, a type of flavonoid renowned for its potent antioxidant and anti-inflammatory properties, along with its ability to enhance the immune system.Objective: This study aimed to elucidate the positive impact of butterfly pea flower extract on atherosclerosis biomarker profiles.Methods: The approach framework used in this research is a true experimental laboratory with a Control Group Post-Test design. Obese male white rats were selected as the research subjects. Thirty-six obese white rats were randomly divided into six groups using the Completely Randomized Design (CRD) method. The data collected were VCAM and IL-6 levels from the blood serum of obese white rats tested using an ELISA photometer. The tunica intima thickness was measured using a microscope. Data were analyzed employing SPSS 18 software, utilizing one-way ANOVA statistical tests and post hoc Tukey tests.Results: The research showed that with the increase in Butterfly Flower Extract (BPFE) dose, there was a consistently lower level of inflammatory biomarkers, such as VCAM-1 and IL-6, compared to positive controls and other variations, as well as tunica intima thickness was thinning than others. A dose of 600 mg/kg BW (P3 group) had VCAM-1 levels up to 30.40 ± 6.71 ng/mL, IL-6 levels up to 17.70 ± 8.29 ng/mL, and tunica intima thickness up to 3.18 ± 1.24 µm.Conclusion: BPFE effectively lowers inflammatory biomarkers and thins the tunica intima thickness in obese white rats at 600 mg/kg BW dose. It may offer promising therapeutic potential in addressing atherosclerosis. 
Complex interaction between allopurinol-induced uric acid reduction and glycemic control: a clinical and molecular study Pongoh, Lucyana; Manoppo, Jonesius Eden; Supit, Gerry; Supit, Alva
Journal of Biomedicine and Translational Research Vol 10, No 3 (2024): December 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i3.22185

Abstract

Background: Diabetes mellitus type 2 (DMT2) and hyperuricemia are two prevalent metabolic diseases worldwide, including in Indonesia. In fact, in the Minahasa tribe, the prevalence of these diseases is among the highest in Indonesia. The interaction between hyperuricemia and DMT2 level is inconclusive, as previous studies about whether allopurinol and its related uric acid reduction correlate with insulin resistance have shown conflicting results.Objective: To examine whether allopurinol-induced uric acid reduction can modify insulin resistance in nondiabetic Minahasan male subjects and study the putative molecular mechanisms of this interaction.Methods: The clinical part of this research was a pseudo-experiment with a pre-test/post-test design. Twenty nondiabetic Minahasan male subjects were subjected to the daily dose of 300 mg allopurinol for three months. Plasma glucose, uric acid, and insulin levels were measured pre- and post-treatment. Homeostatic model assessment of insulin resistance (HOMA-ir) values were calculated by the Oxford HOMA calculator. For the wet lab experiment, the human embryonic kidney cell line was treated with tolerable allopurinol. The expression of glucose transporter 4 (Glut4) mRNA, an insulin-inducible glucose transporter was analyzed by quantitative real-time polymerase chain reaction (qPCR).Results: In nondiabetic Minahasan male subjects, allopurinol administration decreased uric acid serum level, but did not affect plasma glucose and insulin levels. In fact, there is a trend of increasing HOMA-ir among the subjects following allopurinol administration. In vitro, allopurinol treatment also did not increase Glut4 expression, suggesting that allopurinol's effect on diabetes control has other, complex mediative pathways.Conclusion: Allopurinol administration and its related uric acid plasma reduction does not significantly affect insulin resistance; a trend however exists that allopurinol and uric acid reduction increased HOMA-ir. At the molecular level, Glut4 expression is not affected by allopurinol.
Effects of Coal Dust Exposure in Eosinophil and Interleukin (IL)-13 on Pulmonary Remodeling in Asthmatic Mice Models Fujiati, Fujiati; Haryati, Haryati
Journal of Biomedicine and Translational Research Vol 10, No 3 (2024): December 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i3.22243

Abstract

Background: Coal dust is an environmental factor contributing to the risk of respiratory diseases. However, clinical evidence on the effects of coal dust in lung remodelling is currently lacking, especially the effect of eosinophils and IL-13. This study investigates how eosinophil and IL-13 affect pulmonary remodeling in coal dust and asthma combination models.Methods: An experimental study was conducted using BALB/c mice aged 6 to 12 weeks with a weight range of 20-25 g. There were 10 mice in each of the three treatments. Groups were sensitized with normal saline, ovalbumin (OVA)-sensitized, and OVA-sensitized + coal dust. The parameters of lung remodeling (the thickness of epithelium, smooth muscle thickness, the number of goblet cells, and subepithelial fibrosis) and the number of eosinophils were measured with histomorphometry analysis. Total IL-13 concentrations were measured using an IL-13 ELISA kit. The data group of a combination of OVA + coal dust was analyzed using the path analysis method.Results:  From path analysis, it was found that Eosinophils (b=0.006; 95%CI=-2.594 to 2.606; p=0.000) had positive, direct, and statistically significant effects on IL-13. Eosinophil's indirect effect on epithelium thickness and subepithelial fibrosis thickness via IL-13. Interleukine-13 had positive, direct, and statistically significant effects on epithelium thickness (b=0.67; 95%CI=-0.129 to 1.471; p=0.010) and subepithelial fibrosis thickness (b=0.682; 95%CI=0.301 to 1.062; p=0.000).Conclusion: Eosinophils' indirect effect on lung remodeling via IL-13 and IL-13 directly affects airway remodeling, especially epithelium and subepithelial fibrosis components.
Long-Term Effects of Low-Dose Chlorpyrifos Exposure on Serum Albumin Levels in Male Wistar Rats Wisudanti, Desie Dwi; Hidayat, Noval; Jauhani, Muhammad Afiful
Journal of Biomedicine and Translational Research Vol 10, No 3 (2024): December 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i3.22240

Abstract

Background: Chlorpyrifos is one of the organophosphate pesticide types frequently utilized as a pest control agent in Indonesia. Despite its effectiveness in combating pests, the residue levels of chlorpyrifos in the environment and plants have raised serious concerns. Long-term accumulation of chlorpyrifos in the body can lead to organ damage, particularly in the liver and kidneys, which may decrease serum albumin levels.Objective: To investigate the impact of low-dose chlorpyrifos exposure over time on serum albumin levels in Wistar rats.Methods: Thirty male Wistar rats were divided into five groups: the normal control group (Kn) received normal saline solution (+5% Tween 20) orally for 56 days, while the treatment groups (K1, K2, K3, and K4) were administered chlorpyrifos at a dose of 5 mg/kg body weight for 7 days (acute), 14 days (subacute), 28 days (subchronic), and 56 days (subchronic) orally. Serum albumin levels were measured using the dye-binding method with a spectrophotometer.Results: The measurement results indicate that the normal control group (Kn) had the highest serum albumin levels (4.326±0.519 g/dL). Serum albumin levels decreased in the groups treated with chlorpyrifos. The longer the chlorpyrifos exposure, the lower the serum albumin levels. The lowest serum albumin levels were found in group K4 with chlorpyrifos exposure for 56 days (2.826±0.358 g/dL). Statistical analysis using One-way ANOVA and Post Hoc LSD tests showed significant differences (p<0.05) between all treatment groups (K1, K2, K3, and K4) and the control group (Kn).Conclusion: This study shows that administering low-dose chlorpyrifos over a period of 7 to 56 days has a significant effect in reducing serum albumin levels in Wistar rats. The clinical implications of this decrease in serum albumin levels need to be considered in the context of exposure to organophosphate pesticide residues in humans.
Visceral Adiposity Index and Insulin Resistance in Diabetes Mellitus Type 2 Hendrianingtyas, Meita; Pradati, Subandhini Arika
Journal of Biomedicine and Translational Research Vol 10, No 3 (2024): December 2024
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v10i3.23505

Abstract

Background: Diabetes mellitus (DM) has been increasing worldwide. Obesity, especially central obesity, is a sign of increased visceral fat in various diseases, especially DM. Individuals with visceral fat compromise insulin resistance and metabolic disorders and develop diabetes. The gold standard for assessing insulin resistance is the hyperinsulinemic-euglycemic clamp (HEC). However, this method is invasive and expensive, so the homeostasis model assessment-insulin resistance (HOMA-IR) and the quantitative insulin sensitivity check index (QUICKI) are more straightforward, more practical, and invasive measurement methods. Visceral adiposity index (VAI) was developed as a new indicator of visceral adipose tissue dysfunction that can be used for CT examination as a marker of visceral adiposity.Objective: To determine the correlation between visceral adiposity index and insulin resistance in patients with type 2 diabetes mellitusMethods: The study was conducted as an analytical observational study using a cross-sectional design approach at the Diponegoro National Hospital Semarang from March to April 2022. Data analysis using the Pearson test (meaningful if p < 0.05)Results: From the 70 samples examined, there was a correlation between VAI and HOMA-IR (r=0,480; p=<0,001), VAI and QUICKI (r=-0,475; p=<0,001), and a strong correlation between HOMA-IR and QUICKI (r=-0,892; p=<0,001)Conclusion: There was a moderate positive correlation between VAI and HOMA-IR and a moderate negative correlation between VAI and QUICKI. Meanwhile, the results of the correlation test between HOMA-IR and QUICKI show that there is a robust negative correlation. 
Effects of Vitamin C Supplementation on Histology of Callus Diameter and Osteoblast Number in Male Wistar Rats With Complete Femur Bone Fracture Rambe, Syahrul Ramadan; Putra, Reza Mahruzza; Rahmadhany, Heru
Journal of Biomedicine and Translational Research Vol 11, No 1 (2025): April 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i1.25888

Abstract

Background: The role of nutritional factors, particularly vitamin C, in bone repair has been extensively studied. However, despite this research, the specific impact of vitamin C on bone fracture healing remains unclear. While some studies suggest that vitamin C supplementation may enhance bone repair, others report no significant benefits.Objective: This study aimed to investigate the effects of different doses of vitamin C on callus formation and osteoblast proliferation in a rat femur fracture model.Methods: A post-test-only control group design was employed in this study, involving 27 male Wistar rats that were randomly divided into three groups. The first and second groups received daily intramuscular injections of vitamin C at doses of 200 mg/kg body weight (BW) and 500 mg/kg BW, respectively, following femur bone fracture and fixation. The control group did not receive vitamin C and underwent no fixation. After 14 days, all rats were euthanized, and their femur bones were histologically examined for callus diameter and osteoblast count.Results: Vitamin C supplementation significantly increased the callus diameter in rats with complete femoral fractures. Both the 200 mg and 500 mg doses proved effective, demonstrating a clear dose-response relationship. Additionally, Vitamin C significantly elevated the number of osteoblasts, which play a crucial role in bone formation. However, there was no statistically significant difference in osteoblast count between the 200 mg and 500 mg doses.Conclusion: In conclusion, vitamin C supplementation has been shown to positively influence bone fracture healing in rats by promoting an increase in callus diameter and enhancing osteoblast proliferation. This study indicates that vitamin C could serve as a beneficial adjunct therapy for facilitating bone fracture healing, particularly by improving callus formation. Physicians should consider integrating vitamin C into treatment plans for patients with fractures, using doses similar to those applied in this study, adjusted appropriately for human use.
Relationship between MC4R rs17782313 Polymorphism and Body Mass Index and Appetite Regulation in Young Adults in Indonesia Sidarta, Erick; Kumala, Meilani
Journal of Biomedicine and Translational Research Vol 11, No 1 (2025): April 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i1.26018

Abstract

Background: Obesity is an increasing public health problem, including among young adults in Indonesia. Genetic, environmental, and behavioral factors contribute to obesity. One of the genetic variants associated with obesity is the MC4R rs17782313 polymorphism, which plays a role in appetite regulation and energy balance.Objective: This study aims to determine the relationship between the MC4R rs17782313 polymorphism and Body Mass Index (BMI) in young adults in Indonesia and to evaluate its effect on feelings of hunger, satiety and eating satisfaction.Method: We conducted a cross-sectional study involving 152 young adults at Universitas Tarumanagara. Genotyping was done using the real-time PCR method with the KASP™ system. To evaluate hunger, satiety, and eating satisfaction, we used the Visual Analog Scale (VAS; 0–100 mm, where lower scores indicate intense sensations of hunger, satiety, and satisfaction) before and after meals and calculated the difference scores. We analyzed the data using R software.Results: The results showed no significant relationship between the MC4R rs17782313 polymorphism and BMI (p = 0.7). However, there was a significant difference in hunger scores between the TT and TC genotypes (p = 0.024), where individuals with the TT genotype experienced a greater reduction in hunger after eating than individuals with the TC genotype. In addition, we also found a significant difference in BMI based on gender in our young adult population (p = 0.0032).Conclusion: This study found that MC4R rs17782313 was not significantly associated with BMI in this population. However, the variant may influence appetite regulation, as individuals with the TT genotype showed a smaller reduction in hunger after eating. A significant BMI difference between sexes was also observed; these findings suggest a role for MC4R in eating behavior, warranting further investigation in larger, diverse populations.
Effect of Moringa Oleifera on Tumor Necrosis Factor-Alpha and Extracellular Matrix Trabecular Meshwork of Glaucoma Model Wistar Rats Maharani, Maharani; Astuti, Ratih Kusuma; Rahmi, Fifin Luthfia; Prihatningtias, Riski; Istiadi, Hermawan; Wildan, Arief
Journal of Biomedicine and Translational Research Vol 11, No 1 (2025): April 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i1.24127

Abstract

Background: Elevated intraocular pressure (IOP) induces inflammation in the trabecular meshwork (TM) through increases in tumor necrosis factor-alpha (TNF-α) expressions by activating nuclear factor kappa B (NF-κB). Mechanical injury to the TM, such as increased IOP, can cause extracellular matrix (ECM) remodeling, impacting the permanent outflow of aqueous humor. Moringa oleifera (MO) leaf extract is an anti- inflammatory agent that inhibits NF-κB activation and has been studied to have antioxidant effects.Objective: to analyze the effects of MO leaf extract on TNF- α expression and the thickness of the trabecular meshwork ECM in a Wistar rat glaucoma model. Methods: Fourteen male Wistar rats were divided into two groups (n=7): the glaucoma group received oral MO leaf extract at 300 mg/kg body weight for four weeks, and the control group. TNF-α expressions were measured by immunohistochemical stain, and the thickness of the ECM was examined with hematoxylin eosin staining. P-values of less than 0.05 were considered to represent statistical significance.Results: The mean of TNF-α expressions in the treatment group was 4.29 ± 0.76, and the control group was 5.29 ± 0.49. There was a significant difference in TNF-α expressions between the two groups (p=0.015). The mean thickness of the ECM TM experimental group and control group were 155.65 ± 28.72μm and 218.63 ± 40.85μm respectively. The extracellular matrix TM experimental group was statistically significantly thinner than the control group (p=0.006).Conclusion: MO could protect TM from damage caused by high IOP by suppressed TNF- α expression and ECM thickness in the TM Wistar glaucoma model. 
Genetic Counselling Empowers Parents of Children with Intellectual Disabilities : A Fragile X Syndrome Perspective Amir, Mentari; Aulia, Siti Farhanah; Ranakusuma, Octaviani Indrasari; Faradz, Sultana MH
Journal of Biomedicine and Translational Research Vol 11, No 1 (2025): April 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i1.25938

Abstract

Background: Intellectual disabilities (ID) profoundly affect individuals and their families, leading to financial strain, emotional distress, and limited access to healthcare and education. Fragile X Syndrome (FXS), the most common inherited cause of ID, remains largely underdiagnosed in Indonesia due to limited awareness and resources. Objective: This study aimed to assess the impact of genetic counselling on parental empowerment. Methods: This pre-experimental study employed a before and after counselling. A total of 238 parents of children with ID from four special schools in Jakarta participated. Data were collected using the Genetic Counselling Outcome Scale-24 (GCOS-24), which assesses empowerment. Purposive sampling was applied, and data were analysed using paired t-tests and one-way ANOVA. Results: The mean GCOS-24 score increased significantly from 106.79 (SD = 16.36) before counselling to 125.11 (SD = 15.42) after counselling (p < 0.001). Only 27.3% of parents were aware of genetic disorders, reflecting their limited baseline knowledge. A one-way ANOVA analysis showed a statistically significant difference in GCOS-24 score improvements based on parental education level (F=4.035, p=0.008) with parents with primary school education showing the greatest improvement compared to those with high school (p < 0.05). Conclusions: Genetic counselling significantly enhanced parent empowerment, as evidenced by increased GCOS-24 scores. These findings emphasize the importance of expanding genetic counselling services and educational initiatives in Indonesia to improve awareness and support for families managing ID.

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