cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota semarang,
Jawa tengah
INDONESIA
Journal of Biomedicine and Translational Research
Published by Universitas Diponegoro
ISSN : -     EISSN : 25032178     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Journal of Biomedicine and Translational Research (JBTR) is an open access, international peer-reviewed journal that considers articles on: clinical medicine, molecular medicine, tropical medicine, infectious diseases, cardiovascular medicine, molecular biology, genetics, immunology, microbiology, biochemistry, and pharmacotherapy with particular interest on the link between clinical and basic research called translational research.
Arjuna Subject : -
Articles 173 Documents
 14   15   16   17   18 
Successful Management of Post-Excisional Biopsy Dehisced Wound with 1% Framycetin Sulphate and Ozonated Oil Adjuvant: A Case Report Mawu, Ferra Olivia; Sondakh, O. Reymond L.; Tampi, Joan Alexandra; Christopher, Paulus Mario
Journal of Biomedicine and Translational Research Vol 11, No 1 (2025): April 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i1.25956

Abstract

Background: Wound dehiscence is wound edges separation due to disrupted wound healing. Wound dehiscence is a complication in 8% of dermatologic surgeries. In this case, secondary infection of the wound occurred, 1% framycetin sulphate tulle was then chosen to interfere microbial protein synthesis, combined with ozonated oil as adjuvant therapy. Ozone oxidizes bacterial phospholipids and lipoproteins, promotes local tissue metabolism, stimulates fibroblast proliferation, facilitates collagen fiber formation, and supports angiogenesis. This case report described a post-excisional biopsy dehisced wound that was treated with 1% framycetin sulphate tulle and ozonated oil, and this case is the first to report a successful management of post-excisional biopsy dehisced wound with ozonated oil as adjuvant. Case Presentation: An 11-year-old female was brought with a purulent wound on her head post-excisional biopsy. Examination of the parietal region showed a solitary ulcer, 1 cm in diameter, irregular edge, granulated tissue base, serous exudate, crusting, edema, and pus. Treatment was 0.9% NaCl compress, 1% framycetin sulphate tulle, and ozonated oil once weekly. Evaluation on day 21 showed ulcer size reduction and on day 28, ulcer turned into a scar, treatment was continued with mometasone 0.1% cream. Day 86 showed secondary cicatricial alopecia. Complications of a wound in hair-bearing area can occur, in this case, secondary cicatricial alopecia.Conclusion: This paper highlights the utilization of ozonated oil as an adjuvant therapy for a favorable outcome in wound healing.
Detection of Pathogenic Leptospira in Sputum of Leptospirosis Patient with Pulmonary Hemorrhage. Handayani, Farida Dwi; Novipuspitasari, Lisa; Ahmed, Ahmed; Safari, Dodi; Hidajat, Muhammad Choirul; Soebandrio, Amin; Gasem, Muhammad Hussein
Journal of Biomedicine and Translational Research Vol 11, No 2 (2025): August 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i2.28259

Abstract

Background: The pathogenic Leptospira species is the causative agent of leptospirosis, an endemic zoonotic disease in Indonesia. Misdiagnosis of the disease frequently occurs, as confirmatory diagnosis confined to highly specialized laboratories. As well, the pulmonary involvement of leptospirosis with hemoptysis is scarcely reported.Case Presentation: A 49 years-old male patient was admitted to the district hospital with acute febrile illness and a history of traveling to a malaria-endemic area in Borneo, Indonesia. Based on a chest X-ray result, the patient was clinically suspected to have pulmonary tuberculosis. However, the clinical manifestations of leptospirosis i.e. conjunctival suffusion, calf pain, and oliguria were present, and later hemoptysis was also reported. A clinical diagnosis of leptospirosis with pulmonary involvement was proposed. Immunochromatographic test (ICT)-rapid test for vivax/falciparum malaria and Ziehl-Neelsen (ZN) staining of sputum for tuberculosis results were both negative. Microscopic Agglutination Test (MAT), the IgM anti-Leptospira rapid test (lateral flow assay), and PCR amplification of both conventional and real-time (qPCR) were performed using various samples (serum, urine, and sputum). The MAT of acute single serum sample and rapid test were negative. Intriguingly, the PCR showed positive results in sputum and urine samples but not in the serum sample, highlighting the usefulness of leptospiral molecular detection to confirm further diagnosis.Conclusion: Molecular detection of pathogenic Leptospira in sputum samples can be considered for confirmatory diagnosis of leptospirosis patients with pulmonary hemorrhage. Likewise, the urine sample can be used as an option in the examination of severe leptospirosis.
Effect of Umbilical Cord Cell Isolate Administration on STRO-1 and CD 34 Expression of Dental Pulp Stem Cell Culture Nugraeni, Yuli; Rachmawati, Ranny; Az Zahra, Akyla Oktriza; Nugraha, Adelia Adzra
Journal of Biomedicine and Translational Research Vol 11, No 2 (2025): August 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i2.26269

Abstract

Background: Dental Pulp Stem Cell (DPSC) is one of the ideal mesenchymal stem cells (MSCs) for the repair of damaged cells, as it has great proliferative potential and the ability to regenerate pulp-like complex dentins. However, DPSC has limitations in providing stem cell sources due to its small number. Therefore, it is induced with umbilical cord cell isolate (hUC-MSC) which is currently widely developed because of its advantages that are easy to obtain, have proliferative potential, and have anti-inflammatory effects. However, its influence and effectiveness on Dental Pulp Stem Cells is not widely known.Objective: To prove the effect of umbilical cord cell isolate on the expression of Stro-1 and CD 34 in Dental Pulp Stem Cell (DPSC) culture.Methods: This study is experimental laboratory with simple random sampling which is divided into 4 groups, namely 2 control groups and 2 groups of umbilical cord isolate administration which are observed for 12 hours and 24 hours. Observation of expression by immunohistochemical staining was then calculated using Qupath software in 5 fields of view.Results: The treatment group that was given umbilical cord cell isolate showed the highest increase in Stro-1 and CD 34 expression at 24-hour observation compared to the untreated group.Conclusion: There was an increase in the expression of Stro-1 and CD 34 in Dental Pulp Stem Cell cultures after being given umbilical cord cell isolate.
VEGF mRNA Expression in Epithelial Ovarian Cancer: Correlation with rs699947 Gene Variant Prameswari, Yuda Nabella; Suryandari, Dwi Anita; Sukmawati, Dewi; Yunaini, Luluk; Kodariah, Ria
Journal of Biomedicine and Translational Research Vol 11, No 2 (2025): August 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i2.26156

Abstract

Background: Angiogenesis is the formation of new blood vessels, is crucial for cancer growth and metastasis, including in epithelial ovarian cancer (EOC). Vascular Endothelial Growth Factor (VEGF) regulates angiogenesis, and its elevated mRNA expression is linked to poor prognosis in cancer. Genetic variations, such as the rs699947 polymorphism in the VEGF gene, can affect VEGF expression and contribute to cancer progression.Objective: The primary aim of this study is to examine the distribution of the VEGF rs699947 polymorphism and its correlation with VEGF mRNA expression levels in patients with low-grade and high-grade EOC at Dr. Cipto Mangunkusumo Hospital, Indonesia.Methods: This research is a cross-sectional analysis involving 65 normal female whole blood samples and a total of 80 ovarian cancer biopsy samples, including 15 ovarian cysts as expression calibrators, along with 36 low-grade and 29 high-grade EOC samples. The distribution of genotypes and alleles of the VEGF rs699947 polymorphism was assessed through ARMS PCR analysis, while VEGF mRNA expression was quantified using real-time qPCR.Results: Significant differences were observed in both genotype (p<0,01) and allele (p=0,000) distributions between the normal and cases group. The relative mRNA expression of VEGF was significantly elevated in both low-grade and high-grade EOC. Individuals with the homozygous VEGF rs699947 AA genotype exhibited the highest mRNA expression compared to other genotypes. In contrast, individuals carrying the CC genotype showed the lowest correlation with VEGF mRNA expression in both low-grade and high-grade EOC.Conclusion: This study shows that the A allele of VEGF rs699947 is correlated with increased VEGF mRNA expression in EOC patients, particularly in those with the AA genotype. Conversely, the C allele may offer a protective effect against EOC, as the CC genotype is linked to lower VEGF mRNA expression. Genetic screening for VEGF rs699947 could facilitate early detection and inform targeted therapeutic strategies.
The Association Between Intake of Saturated, Monounsaturated, and Polyunsaturated Fatty Acids with Bifidobacterium Abundance Among Obese Adults Without Metabolic Syndrome Rustanti, Ninik; Sinthika, Euodia; Lestari, Endang Sri
Journal of Biomedicine and Translational Research Vol 11, No 2 (2025): August 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i2.26630

Abstract

Background: Bifidobacterium is a key gut microbe that contributes to host metabolism, immunity, and intestinal integrity through SCFA production. Dietary fats are known to modulate gut microbiota, but evidence on the effects of specific fat types—SFA, MUFA, PUFA—on Bifidobacterium in obese adults without metabolic syndrome remains limited.Objectives: To examine the association between intake of dietary fat types and the abundance of Bifidobacterium among obese adults without metabolic syndrome.Methods: A cross-sectional study was conducted in Semarang, Indonesia, involving 60 obese adults (BMI ≥ 25 kg/m²) without metabolic syndrome. Dietary intake was assessed using a validated SQ-FFQ, and Bifidobacterium abundance was quantified using qPCR from fecal samples. Correlation and multivariate linear regression - adjusted for age, sex, and energy intake - were used to assess associations between variables.Results: Saturated fat intake was moderately and negatively correlated with Bifidobacterium levels (r = –0.464; p < 0.001), while total fat intake also showed a statistically significant, but weaker, negative correlation (r = –0.346; p = 0.007). PUFA intake showed a weak but statistically significant positive correlation (r = 0.269; p = 0.037), whereas MUFA intake was not significantly associated. Multivariate analysis identified SFA as an independent negative predictor of Bifidobacterium abundance.Conclusion: High intake of saturated fat is associated with decreased Bifidobacterium levels even in obese adults without metabolic syndrome, whereas PUFA may exert modest protective effects. These findings suggest that the type of dietary fat, rather than its quantity, plays a key role in modulating gut microbiota composition.
Sulforaphane Promotes Adipocyte Thermogenesis: Molecular Insights, Anti-Obesity Potential, and Future Perspective Widayanti, Iftitan Setya; Noer, Etika Ratna; Fulyani, Faizah
Journal of Biomedicine and Translational Research Vol 11, No 3 (2025): December 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i3.28102

Abstract

Over the past five decades, the increasing incidence of obesity has sparked considerable interest in nutraceuticals as promising natural alternatives for effective weight management and metabolic health improvement. Sulforaphane (SFN), an isothiocyanate abundant in cruciferous vegetables, has gained attention for its potential in obesity management, particularly by promoting the browning of white adipose tissue (WAT) and transforming it into energy-burning fat. While its potential is significant, the underlying molecular mechanisms are complex and require a comprehensive synthesis. Therefore, this review explores sulforaphane (SFN) as a potential nutraceutical alternative by examining the scientific evidence of its anti obesity effects, focusing on its ability to activate multiple signalling pathways, including Nuclear Factor Erythroid 2-related factor 2 (Nrf2), AMP-activated protein kinase (AMPK), and sirtuin 1 (SIRT1), as well as upregulation of uncoupling protein 1 (UCP1). These are the key pathways in mitochondrial biogenesis, lipid metabolism, and thermogenesis. Additionally, SFN can mitigate oxidative stress and modulate inflammatory responses, further contributing to improved metabolic function and energy expenditure. While much of the research on SFN has focused on its effects on mature cruciferous vegetables, current research increasingly directs attention to microgreens, which contain significantly higher concentrations of bioactive compounds, including SFN. This review highlights SFN’s molecular mechanisms underlying its role in the context of obesity, specifically its effects on WAT browning, metabolic regulation, and thermogenesis. We also explored the potential of microgreen-derived SFN as a promising nutraceutical for obesity intervention and metabolic regulation, highlighting the novel bioactive chemical and biological properties of these plants.
Exploring the Potential of Yoga as a Complementary Therapy for Polycystic Ovarian Syndrome (PCOS) Kumalasari, Anatalya Diah Ayu; Faiza, Agyta Hanifa; Husodho, Gabriela Valencia Putri; Kesoema, Tanti Ajoe
Journal of Biomedicine and Translational Research Vol 11, No 3 (2025): December 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i3.29516

Abstract

Polycystic ovarian syndrome (PCOS) is an endocrine and metabolic disorder that is most commonly experienced by women. This condition can reduce the quality of life because it can affect physical, psychological, emotional, social, and financial. The first-line and safest therapy for PCOS is lifestyle changes. One of them is doing physical activity. Yoga as one of the low-intensity physical activities has the potential as a complementary therapy for PCOS. This study implements a systematic review with qualitative analysis method. A literature search was done using the search engines PubMed, Science Direct, and Proquest by setting inclusion criteria and exclusion criteria. Yoga is a sport that can be done by everyone because it does not require fitness and flexibility at a certain level. Some of the mechanisms of yoga as complementary therapy for PCOS are the optimization of the autonomic nervous system, regulation of the endocrine system, reduction of stress levels, and balancing of neural hormones. Previous research has shown that yoga can improve various aspects in PCOS women such as anthropometric parameters, menstrual cycle, ovarian morphology, biochemical markers, hormones, cardiovascular, and psychiatric conditions. Yoga can improve various aspects including anthropometric parameters, menstrual cycle, ovarian morphology, biochemical markers, hormones, cardiovascular, and psychiatric conditions.
Association Between Friedman Tongue Position and Respiratory Disturbance Index in Patients with Obstructive Sleep Apnea Herlina, Sari Nafa; Yunika, Kanti; Suryawati, Herlina
Journal of Biomedicine and Translational Research Vol 11, No 3 (2025): December 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i3.27117

Abstract

Background: Obstructive sleep apnea (OSA) is characterized by repeated episodes of complete or partial upper airway obstruction during sleep. This sleep disorder is often ignored or undiagnosed because it is often underdiagnosed due to non-specific symptoms, even though it can cause health problems and interfere with the quality of life. The gold standard for diagnosing OSA is polysomnography (PSG), but PSG is not available in all healthcare facilities. The Friedman Tongue Position (FTP) examination is a simple method for estimating upper airway obstruction and is expected to serve as a screening tool for OSA risk.Objective: To determine the association between Friedman Tongue Position and the Respiratory Disturbance Index (RDI) in obstructive sleep apnea. To determine the association between body mass index, age, and sex with the respiratory disturbance index in obstructive sleep apnea.Methods: The study was conducted from August to October 2024 at Dr. Kariadi Central General Hospital Semarang, with a total sample of 44 adults (>18 years), comprising 22 OSA and 22 non-OSA subjects. This was a cross-sectional study involving patients who had undergone PSG. The RDI values were obtained from PSG results. FTP was assessed through tongue examination. Data were analyzed using the Chi-square test.Results: Chi-square analysis revealed significant associations between BMI (p = 0.013) and FTP (p < 0.001) and RDI. Meanwhile, Fisher’s Exact test for age (p=0.697) and Chi-square test for sex (p=0.203) indicated no significant association with RDI.Conclusion: FTP and BMI showed significant associations with RDI, where higher FTP grades and obesity increased the likelihood of OSA. In contrast, age and sex were not significantly associated with RDI, indicating that they do not independently influence respiratory disturbance severity.
Combination Effect of Slow Interval Training and Lemongrass Ethanol Extract (Cymbopogon citratus) on Body Weight and Fat Mass in Male Wistar Rats (Rattus norvegicus Sp) Obesity Model Siahaan, Sulyaprilawati Battri; Machrina, Yetty; Widyawati, Tri; Rusdiana, Rusdiana; Savira, Maya
Journal of Biomedicine and Translational Research Vol 11, No 3 (2025): December 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i3.28284

Abstract

Background: Obesity, a global health crisis affecting over 890 million adults, requiring effective and safe management strategies. Lifestyle-based interventions, such as physical exercise and herbal supplementation, provide promising non-invasive alternatives. Slow Interval Training (SIT) improves fat metabolism with low injury risk, while lemongrass (Cymbopogon citratus) contains bioactive compounds with anti-obesity potential.Objective: This study investigates the combined effects of SIT and lemongrass ethanol extract on body weight and fat mass in a high-fat diet (HFD)-induced obese male Wistar rat model.Methods: Thirty-five male Wistar rats were divided into five groups: healthy control, obese control, SIT only, lemongrass extract only, and SIT + lemongrass. Obesity was induced through a high-fat diet before intervention. Body weight was measured weekly, and fat mass was analyzed after eight weeks of treatment.Results: Obese controls showed significant weight gain (p<0.001). The lemongrass-only group showed a 12.7% weight reduction, while the combination group achieved the lowest final weight (200 g) and the lowest fat mass (2.39 g, p=0.048). Significant intergroup differences appeared from week 1 and persisted until week 13 (p<0.05). The combination group maintained stable weight after intervention, outperforming SIT or lemongrass alone.Conclusion: The combination of SIT and lemongrass ethanol extract significantly reduces body weight and fat mass in obese rats, demonstrating synergistic efficacy. Lemongrass inhibits fat absorption and enhances metabolic regulation, while SIT promotes fat oxidation. This dual approach offers a viable, non-invasive strategy for obesity management, warranting further clinical exploration.
Low sEng Level in Preeclampsia with MFTHFR Gene Polymorphism Suggesting a Protective Factor Pramono, M. Besari Adi; Faradz, Sultana MH; Suhartono, Suhartono; Susanto, Hardhono; Rachmawati, Banundari; Pramono, Noor
Journal of Biomedicine and Translational Research Vol 11, No 3 (2025): December 2025
Publisher : Faculty of Medicine, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jbtr.v11i3.29375

Abstract

Background: Preeclampsia is one of the most serious complications of pregnancy and the leading cause of maternal and fetal mortality. Various studies have shown that Glutathione Peroxidase (GPx) deficiency and increased Soluble endoglin (sEng) level are consistently associated with the incidence of preeclampsia. Several studies also show the role of MTHFR A1298C and C677T gene polymorphisms in preeclampsia.Objective: This study investigated association between blood GPx, sEng levels, MTHFR A1298C and C677T gene polymorphisms in Preeclampsia.Methods: This analytic observational case-control study was conducted on 70 cases of preeclampsia and 70 controls. Blood GPx and sEng levels were measured using Enzyme Linked Immunosorbent Assay (ELISA). MTHFR A1298C and C677T gene polymorphism was genotyped using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The data obtained were analyzed using the Mann-Whitney U test, chi-square test, and independent T-test.Results: There were no significant differences in GPx levels or MTHFR A1298C/C677T genotype distribution between groups. sEng levels were significantly higher in the preeclampsia group than controls (p=0.001). ROC analysis identified a cut-off of 7.75 ng/mL. Among preeclampsia patients, those with the MTHFR 1298AC/CC genotypes had lower sEng levels than wildtype (p=0.027), suggesting a potential protective effect. No association was found for C677T.Conclusion: We found no significant difference in GPx level, MTHFR A1298C and C677T gene polymorphism between preeclampsia and control group. Soluble endoglin (sEng) level in the preeclampsia group (mean: 11.0±5.22) were significantly different (p=0.001) compared to the control group (mean: 8.1±5.31). Increased level of sEng is associated with incidence of preeclampsia. A key finding in this study is the significantly lower sEng levels observed in preeclampsia patients carrying the MTHFR 1298AC and 1298CC alleles compared to the control group (p=0.027). This indicates a protective factor where in preeclampsia with MTHFR gene alleles 1298AC and 1298CC sEng levels are lower compared to wildtype.

Page 17 of 18 | Total Record : 173

 14   15   16   17   18 

Filter by Year

2015 2025


Reset
Filter By Issues
All Issue Vol 11, No 3 (2025): December 2025 Vol 11, No 2 (2025): August 2025 Vol 11, No 1 (2025): April 2025 Vol 10, No 3 (2024): December 2024 Vol 10, No 2 (2024): August 2024 Vol 10, No 1 (2024): April 2024 Vol 9, No 3 (2023): December 2023 Vol 9, No 2 (2023): August 2023 Vol 9, No 1 (2023): April 2023 Vol 8, No 3 (2022): December 2022 Vol 8, No 2 (2022): August 2022 Vol 8, No 1 (2022): April 2022 Vol 7, No 3 (2021): December 2021 Vol 7, No 2 (2021): August 2021 Vol 7, No 1 (2021): April 2021 Vol 6, No 3 (2020): December2020 Vol 6, No 2 (2020): Augusts 2020 Vol 6, No 1 (2020): April 2020 Vol 5, No 2 (2019): December 2019 Vol 5, No 1 (2019): July 2019 Vol 4, No 2 (2018): December 2018 Vol 4, No 1 (2018): July 2018 Vol 3, No 2 (2017): December 2017 Vol 3, No 1 (2017): July 2017 Vol 2, No 2 (2016): December 2016 Vol 2, No 1 (2016): July 2016 Vol 1, No 2 (2015): December 2015 Vol 1, No 1 (2015): April 2015 More Issue