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Bioeksperimen: Jurnal Penelitian Biologi
Published by Universitas Muhammadiyah Surakarta
ISSN : -     EISSN : -     DOI : -
Core Subject : Education, Agriculture,
Agriculture, Biological Sciences & Forestry Education
Arjuna Subject : -
Articles 484 Documents
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PERBANDINGAN KEMAMPUAN MIGRASI ADIPOSE-DERIVE STEM CELLS ASAL MANUSIA PADA BERBAGAI MEDIUM PERTUMBUHAN Rosadi, Imam; Karina, Karina; Rosliana, Iis; Widyastuti, Tias; Wahyuningsih, Komang A; Sobariah, Siti; Afini, Irsyah
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10432

Abstract

Cell migration is natural process that plays an important role in development of organisms. Stem cells are an important in the cells migration process due to repairing tissues or organs. Stem cells ability studies towards cell migration process have been developed and studied. In this study, adipose-derived stem cells (ADSCs) were used as cell models of stem cell migration which cultured on medium containing 10% fetal bovine serum (FBS), a combination of 1 0% FBS and L-ascorbic acid (LAA), 10% human platelet-rich plasma (PRP) and 0.1% FBS as a control. The technique that used to analyse cell migration process is scratch assay method. The results of this study indicate that ADSCs were able to migrate on medium containing 10% FBS, 10% FBS-LAA and PRP. On 24 h of observation, the scratched area of ADSCs were 0.70x (control), 0.13x (10% FBS), 0.04x (10% FBS-LAA), and 0.21x (10% PRP) compared to 0 h (1.00x). The width of scratches area in the control group at 24 h was significantly higher than the other treatment groups. This is suggested due to the lack of growth factor contained in the medium as a nutrition for the cells. Therefore, it can be concluded that ADSCs are able to migrate on various medium supplemented by FBS or human PRP.
ANALISIS KEDALAMAN LAUT PURBA BATU GAMPING GORONTALO BERDASARKAN KANDUNGAN FOSIL FORAMINIFERA BENTONIK Permana, Aang Panji; Eraku, Sunarty Suly
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10428

Abstract

The research location is in the form of highland morphology in the Gorontalo City. Research material in the form of limestone with research methods in the form of field surveys and micropaleontological analysis. The purpose of this research is to find out the bentonic foraminifera fossil species and to know paleobathymetry. The results of the analysis there are four fossil species namely Biloculinella depressa, Cornuspira foliacea, Pyrgo laevis and Rhabdammina discreta. Based on the content of bentonic foraminifera fossils, it can be determined that paleobathymetry is Middle Neritic - Outer Neritic (20-200 meters).
TREE COMMUNITY COMPOSITION AND STRUCTURE OF CACAO (THEOBROMA CACAO L.) BASED AGROFORESTRY IN WEST SUMATERA, INDONESIA Santhyami, Santhyami; Basukriadi, Adi; Abdulhadi, Rochadi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10433

Abstract

The aim of this study is to obtain basic descriptive account of structure and floristic composition of cacao based agroforestry in conserving tree species diversity in West Sumatera. An analysis of the composition and structure of three types of cacao based agroforestry system (AFS) [cacao - rubber (CR), cacao ? coconut (CC) and cacao multistrata (CM)] was conducted in April to June 2017. Data collection was done by using quadrat method of 20 x 20 meter plots with a total of 6 plots on each types of AFS. Herbarium specimens were collected and identified. Among all types of these AFS, CC was the most diverse floristically (H? = 1.02) with the highest total of species (10 species), followed by CM (9 species) and CR (7 species). CM has the highest tree density of 1108 trees per ha and the highest total of basal area (30.53 m2). We also did a comparison of the floristic composition of cacao based AFS with the nearest natural forest from previous study. There was a large decrease in the number of non-cocoa tree species from natural forest to AFS, from 139 to 6-9 species. The only native tree species found both in the forest and AFS is Pterospermum javanicum Jungh. It is concluded that three types of cocoa based AFS in West Sumatera were floristically different with natural forest.
TINGKAT KEANEKARAGAMAN HEWAN TROBLOBIONTS PADA EKOSISTEM GUA DI TAJUR BOGOR JAWA BARAT Marhento, Giry; Alamsyah, Mashudi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10429

Abstract

The purpose of this study was to determine the level of diversity of Troglobionts animals, species composition, species dominance, and uniformity in the Cave of Tajur Bogor, West Java. The variables in this study consisted of survey methods by calculating diversity, dominance, uniformity, and species composition to find out what types of animals are in the ecosystem of the cave. Based on the results of research on animal species found there were 10 species of biota belonging to 3 transects with a total of 88 species of cavernous biota. The sample in this study used the 10x10 M line transect method three times in different places. Determination of line transect measurements in this study is based on differences in the environmental zone at the outermost point or cave line which is the starting point of line transect measurement. The results of statistical calculations are obtained as follows: diversity biota index (H ') in the range of 0.4168-0.9684, the index of species composition (P) ranges between 0.2386-0.3861, the uniformity index (E) range between 0,4304-1, and the dominance of type (C) ranges between 5,2118-5,2118. Based on the calculation of diversity, composition, uniformity, and dominance of the type of biota in the area of Tajur Bogor, West Java, at the low-moderate level. Overall the types of biota found in this study were 3 transects, generally producing different amounts during the study, the total biota species found in the cave consisted of 88 individuals from 14 families and 14 species while the highest composition of biota types in transect III where of the 34 species found throughout the transect, 33 were in the second transect and the lowest was in transect I with only 21 species.
PENGARUH PENANGANAN SAMPAH DENGAN SISTEM PENGOMPOSAN TERHADAP BEBAN TEMPAT PEMROSESAN AKHIR SAMPAH Yustikarini, Rahmawati Tri; Setyono, Prabang; Wiryanto, Wiryanto
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.5134

Abstract

Keterbatasan lahan TPA merupakam masalah penting di Kabupaten Magetan yang harus diselesaikan mengingat semakin sulitnya mendapatkan lahan yang baru. Dengan semakin bertambahnya jumlah penduduk maka timbulan sampah yang masuk TPA Milangasri pun semakin bertambah sehingga akan semakin memperpendek umur TPA. Dengan ditetapkannya Peraturan Daerah No. 1 Tahun 2016 tentang Pengolahan Sampah Organik dengan Sistem Pengomposan maka apabila bisa diterapkan secara optimal akan mengurangi jumlah sampah yang masuk ke TPA. Komposisi sampah Kota Magetan 52,59 % terdiri dari sampah organik yang terbagi ke dalam sampah organik basah 34,73 % dan organik kering 17,86 %. Dengan tingginya jumlah sampah organik maka akan memberi peluang untuk dapat dilakukan pengomposan. Pada penelitian ini saat tidak dilakukan pengomposan maka pada Tahun 2025 volume sampah terkompaksi atau beban landfill sebesar 129.030,57 m3 dan luas lahan TPA yang dibutuhkan sebesar 16.129 m2. Saat dilakukan pengomposan maka beban landfill berkurang menjadi 83.869.87 m3 dan luas lahan TPA yang dibutuhkan adalah 10.484 m2. Dengan demikian pengaruh sistem pengomposan bisa mengurangi beban TPA sehingga lahan yang dibutuhkan lebih sedikit dan dapat membuat umur TPA menjadi lebih lama.
STRUKTUR ANATOMIS OVARIUM DAN PERKEMBANGAN BUAH ADAS (Foeniculum vulgare Mill.) Adi, Bayu Nowo; Susanti, Siti
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8909

Abstract

Adas (Foeniculum vulgare Mill.) merupakan tanaman yang digunakan secara luas sebagai tanaman obat dan rempah terutama bagian buah. Pemahaman mengenai struktur anatomis ovarium dan perkembangannya menjadi buah dapat dijadikan sebagai dasar studi untuk pengembangan metode pemanfaatan buah. Penelitian ini dilakukan untuk mempelajari struktur anatomis ovarium dan perkembangan buah adas. Sampel ovarium dan buah adas dalam 9 fase umur difiksasi dengan larutan FAA. Preparat dibuat dengan menggunakan metode parafin dengan pewarnaan tunggal menggunakan safranin 1% dan diamati di bawah mikroskop cahaya menggunakan OptiLab. Analisis data dilakukan dengan analisis kualitatif dan deskriptif. Ovarium bunga adas memiliki dua daun buah yang terdiri atas lapisan jaringan epidermis dan lapisan jaringan parenkim dengan bentuk sel membulat dan ruang antar sel yang sempit. Terdapat lima berkas pembuluh dan vittae pada bagian tengah lapisan parenkim. Masing-masing daun buah terdapat satu kantung embrio dan akan berkembang menjadi satu merikarpium. Eksokarpium berkembang dari epidermis ovarium dan 2-3 lapis sel parenkim di bawahnya, mesokarpium berkembang dari sel parenkim pada bagian tengah, serta endokarpium berkembang dari sel parenkim yang berbatasan dengan ruang ovulum. Eksokarpium dan endokarpium tidak mengalami perkembangan dan menipis di akhir perkembangan buah. Berkas pembuluh dan vittae masih dapat diamati di akhir fase. Berkas pembuluh bermigrasi ke sudut-sudut buah adas dan membentuk rigi.
Pengaruh Tipe Media Pertumbuhan Rhodobacter sp Terhadap Produksi Bakteriorhodopsin Lestari, Eni
Bioeksperimen: Jurnal Penelitian Biologi Vol 7, No 1: Maret 2021
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v7i1.12371

Abstract

Rhodobacter sp merupakan bakteri ungu non sulfur. Bakteri ini menghasilkan pigmen dalam  pertumbuhan secara fotoautotrof. Salah satu pigmen yang dihasilkan adalah Rhodopsin. Rhodopsin merupkan salah satu pigmen penangkap cahaya bagi bakteri ini. Penelitian ini bertujuan untuk melihat intensitas warna pigmen bakteriorhodopsin secara spektrofotometri yang dihasilkan bakteri ini dalam 2 tipe medium yang berbeda, dengan waktu inkubasi selama. 4 hari menggunakan lampu pijar dengan lux 2000. Tipe medium pertumbuhan yang diuji adalah Sea water medium dan Sea water complete medium.  Metode penelitian ada uji kualitatif intensitas warna pigmen bakteriorhodopsin berdasarkan nilai absorbansi pada panjang gelombang 530 nm menggunakan spektrofotometer UV-Vis. Hasil dari penelitian ini adalah nilai absorbansi  rata-rata pada pigmen bakteriorhodopsin yang dihasilkan dari 2 tipe medium pertumbuhan yakni pada Sea water medium didapatkan  nilai absorbansi bakteriorhodopsin adalah 0,067, sedangkan pada Sea water complete medium  didapatkan nilai absorbansi bakteriorhodopsin adalah 0,211. Kesimpulan pada penelitian adalah terdapat perbedaan produksi pigmen Rhodobacter dengan 2 tipe medium pertumbuhan serta medium yang paling baik menghasilkan pigmen bakteriorhodopsin adalah Sea Water Complete Medium.
ANALISIS SENYAWA INHIBITOR ENZIM KATEPSIN KULIT IKAN PATIN TERHADAP PENUNDAAN KEMUNDURAN MUTU CUMI-CUMI Kusuma, Fitriana Dian; Susanti, R.; Anggraini, Septiana; Arlinda, Dyken Dwi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8500

Abstract

Daya simpan cumi-cumi dipengaruhi oleh aktivitas enzim katepsin yang menyebabkan kemunduran mutu cumi-cumi pada hari ke tiga setelah fase post rigor. Aktivitas inhibitor katepsin alami dari ikan patin berpotensi menghambat kemunduran mutu ikan jenis lain. Penelitian ini bertujuan untuk menganalisis senyawa inhibitor enzim katepsin dari kulit ikan patin terhadap kemunduran mutu cumi-cumi. Penelitian didesain dengan rancangan acak dengan pola dua faktorial. Faktor pertama adalah konsentrasi inhibitor enzim katepsin yang diekstrak dari kulit ikan patin, dengan empat variasi yaitu 1:0; 1:1; 2:3; dan 3:2. Faktor kedua adalah lama rendaman/inkubasi cumi-cumi pada fase post rigor selama 3, 7, dan 9 hari dalam ekstrak inhibitor. Setelah direndam, aktivitas enzim katepsin pada cumi-cumi diukur dengan metode spektrofotometri. Setelah didapatkan absorbansi maka aktivitas enzim dan persentase penghambatannya dihitung. Hasil uji Two Way Annova menunjukan bahwa konsentrasi berbeda nyata dengan peresentase penghambatan sedangkan lama penyimpanan tidak berbeda nyata dengan taraf kepercayaan 0,05. Konsentrasi kemudian diuji lanjut dengan Duncan dan konsentrasi yang paling efektif untuk menghambat aktivitas enzim adalah konsentrasi 3:2 dengan persentase penghambatan 57%. 
Efektivitas Pupuk Cair Pseudomonas fluorescens Agensia Pengendali Hayati Terhadap Penyakit Mosaik Tanaman Kakao Probowati, Wiwit; Nugraheni, Ika Afifah; Aryani, Titin
Bioeksperimen: Jurnal Penelitian Biologi Vol 7, No 1: Maret 2021
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v7i1.10245

Abstract

Cocoa is a strategic commodity for plantation in Indonesia. However, pest and disease infections are inevitably common constrains for the cocoa cultivators. Cocoa mosaic disease is one of disease which is cause cocoa trees suffering. Pseudomonas fluorescens are obligate aerobic bacteria that are very valuable for agricultural technology. The bacteria also protects plants from pathogens infection by producing secondary metabolites that kill bacteria and other fungi. The bacteria can used as safe and environmentally friendly biological control agents. This study aims to determine P. fluorescens liquid fertilizer formulation and its effectiveness in suppressing mosaic disease in cocoa plants. The research method was carried out by making a liquid formula from P. fluorescens and then applying it to cocoa plants that were attacked by mosaic disease. Bacterian isolates grown in conch broth medium were incubated for 3 days. Then the fertilizer formula was applied to mosaic symptomatic cocoa plants with fertilizer concentrations of 25%, 50% and 75% compared to spraying using chemical insecticides. The results showed that the liquid formula concentration of 75% can suppress the most effective mosaic symptoms and the optimal growth of cocoa plants for 7 treatments.
UJI AKTIVITAS ANTI BAKTERI EKSTRAK ETANOLIK UMBI AKAR BATU (Coccinia grandis L.Voight) TERHADAP BAKTERI SALMONELLA SP Rukmana, Rizal Maarif; Nugroho, Rahmat Budi; Wisnumurti, Dwi Admani
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 2: September 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i2.8390

Abstract

AbstractSalmonella sp are bacteria that often infect human and animal. This type of bacteria can cause Salmonellosis in human. Previous research has been carried out on secondary metabolites of plants as medicinal ingredients. One of the plants that has potential as a medicine is stone Akar Batu (Coccinia grandis L Voight). This study aims at examining the antibacterial activities of ethanolic extract of stone Akar Batu on Salmonella sp. The extraction method was carried out by maceration and the solvent used was ethanol 96%. Antibacterial activity was conducted using diffusion method. Identification of compound groups of extracts was done by reacting extracts with various chemical reagents. The results showed that stone of Akar Batu powder had a moisture content of 5.99% and a yield of 1.76%. The identification results of ethanolic extract of stone Akar Batu chemical group indicated that the extract contained flavonoids, alkaloids, saponins and tannins. The results of antibacterial activity test have revealed that ethanolic extract of stone Akar Batu can inhibit Salmonella sp.

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