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Bioeksperimen: Jurnal Penelitian Biologi
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Pengaruh Pasta Tomat Terhadap Kolesterol Darah Mencit Nugraheni, Febriani Sarwendah Asri; Jailani, Jailani; Purwati, Sri
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9244

Abstract

This study aims to determine the effect of tomato paste on blood cholesterol in mice. It was an experimental study using a complete randomized study design consisting of four treatments P0, P1, P2, and P3 by giving tomato paste solution in a concentration of 0%, 20%, 40% and 80% and 6 replications. Data were analyzed using Anova one path and continued with BNT with a confidence level of 95%. The results showed that the tomato paste solution affected the blood cholesterol of mice due to Fhit (3.27)> F tab (3.10) and the treatment that gave the greatest effect was on giving the concentration of tomato paste solution by 80%.
Efek Penambahan Limbah Lokal Jerami Dan Sekam Padi Bagi Pertumbuhan Jamur Tiram Putih (Pleurotus ostreatus) Shobah, Afifah Nur; Oktavia, Swastika
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9233

Abstract

White oyster mushroom (Pleurotus ostreatus) is a group of microscopic fungi that are used as food.  P. ostreatus is cultivated in an artificial medium derived from sawdust and has been sterilized. However, the use of sawdust also has problems. Straw and husk of rice can be used as mushroom growing media because they contain organic ingredients such as cellulose, hemicellulose, and lignin. This study aimed to know the growth of white oyster mushroom (P. ostreatus) on straw and husk of rice as an artificial medium and to know the best composition of straw and husk of rice that can be got highly produced of white oyster mushroom (P. ostreatus). The methods of this research were experimental with ten treatments and included several stages including preparation of tools, materials and research sites, the stage of cultivation of P. ostreatus and data collection. This study used an experimental method with a completely randomized design consisting of 40 experimental units. The results obtained were the most optimal P. ostreatus mycelium growth in K1J2S1 treatment with a mean growth rate of 30,60 cm / 30 days, the highest wet weight was K3J1S0 which was 85,83 g while the highest dry weight was in K1J2S1 treatment that is equal to 8,71 g.
Aplikasi Pupuk Organik dari Campuran Limbah Cangkah Telur dan Vetsin dengan Penambahan Rendaman Kulit Bawang Merah terhadap Pertumbuhan Tanaman Cabai Merah Keriting (Capsicum annum L. var. Longum) Noviansyah, Bayu; Chalimah, Siti
Bioeksperimen: Jurnal Penelitian Biologi Vol 1, No 1: Maret 2015
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v1i1.316

Abstract

Limbah cangkang telur yang merupakan salah satu bahan pencemar dapat dimanfaatkan menjadi produk yang lebih bermanfaat salah satunya dalam pembuatan pupuk organik. Hal ini didasarkan pada komposisi cangkang telur yang memungkinkan untuk dikembangkan menjadi pupuk organik Tujuan penelitian ini adalah 1) mengetahui pengaruh pemberian pupuk organik campuran cangkang telur dan vetsin dengan penambahan rendaman kulit bawang merah terhadap pertumbuhan tanaman cabai merah keriting. 2) mengetahui pertumbuhan terbaik dari berbagai perlakuan konsentrasi pupuk pada pertumbuhan cabai merah keriting. Penelitian dilaksanakan di laboratorium Biologi FKIP UMS pada bulan Februari sampai April 2014. Metode yang digunakan dalam penelitian ini adalah Rancangan Acak Lengkap (RAL) dengan satu faktor yaitu konsentrasi pupuk organik dengan 4 perlakuan dan 6 ulangan. Parameter yang diukur adalah tinggi tanaman, jumlah daun, dan biomassa awal dan akhir. Hasil penelitian menunjukkan bahwa konsentrasi pupuk organik limbah cangkang telur, vetsin 5%, 7,5%, dan 10% dengan penambahan rendaman kulit bawang merah memberikan pengaruh baik terhadap pertumbuhan tanaman cabai merah keriting. Dilihat dari parameter tinggi tanaman dan jumlah daun menunjukkan pengaruh signifikan antar perlakuan sedangkan biomassa tanaman tidak menunjukkan perbedaan yang signifikan. Konsentrasi pupuk organik 7,5% memberikan pengaruh paling baik pada pertumbuhan tanaman cabai merah kriting dibanding dengan perlakuan yang lain dan kontrol.
Pengaruh Suhu Terhadap Siklus Hidup Lalat Buah (Drosophila melanogaster) Suharsono, Suharsono; Nuryadin, Egi
Bioeksperimen: Jurnal Penelitian Biologi Vol 5, No 2: September 2019
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v5i2.9240

Abstract

Fruit flies (Drosophila melanogaster) generally have four phases in their life cycle, namely eggs, larvae, pupae and imago. In general, Drosophila melanogaster experiences a life cycle of 8-11 days at optimal temperatures. At lower temperatures the time required to complete its life cycle is relatively longer and slower which is around 18-20 days. Whereas at higher temperatures adult flies that grow will be sterile. The development period of Drosophila melanogaster in its life cycle is influenced by several factors, namely ambient temperature, food availability, level of maintenance density and light intensity. This study aims to determine the effect of temperature on the life cycle of fruit flies (Drosophila melanogaster). The study will be analyzed using quantitative analysis using randomized block design (RBD) with six temperature difference treatments for each treatment and repeated 4 times so that there are 24 experiments. The results showed the development in the Drosophila melanogaster cycle starting from Egg - Larva (Instar I) - Larva (Instar II) - Larva (Instar III) - Pre Pupa - Pupa - Imago. The life cycle of Drosophila melanogaster has an average of 42.08 hours or 1.75 days up to 79.96 hours or 3.33 days in each treatment. And obtained a fast time during the life cycle of Drosophila melanogaster at a temperature of 30°C is 10.47 days and the longest at a temperature of 18°C is 18.35 days.
STUDI PENERAPAN BANK SAMPAH DALAM UPAYA PENGELOLAAN LINGKUNGAN HIDUP DI KOTA YOGYAKARTA Haryanti, Sri; Gravitiani, Evi; Wijaya, Mahendra
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10434

Abstract

Waste Bank program is an alternative in waste management by 3R (Reduce, Reuse, Recycle) approach. Yogyakarta is one of the cities that has implemented that program. This study was aimed to analyze the composition of the deposited waste to the banks and examine the implementation of waste banks in Yogyakarta city in environment management efforts. The study was conducted in October-December 2016, and  is a quantitative study that was conducted by measurement, interview and observation methods in five waste banks in Yogyakarta City. Data analysis was performed by means of quantitative descriptive in the form of tables and graphs. The results show that total weight of the deposited waste in a month is 3,062.1 kg, in average. The composition of the waste consists of: plastic 23.36%, paper 65.06%, glass 5% and metal 6.56%. The Waste Bank program in Yogyakarta City is able to assist the efforts of environment management, with a potentiton of 97%  waste reduction. After the existence of waste banks, in corresponds with their environment condition, 65 respondents (65%) stated it is clean, and 35 (35%) stated very clean. Respondents who stated that their houses are very clean and clean are 34% and 66%, respectively.
ISOLASI DAN KARAKTERISASI BAKTERI INDIGEN PENGURAI LEMAK PADA LIMBAH CAIR BATIK TULUNGAGUNG Fidiastuti, Hasminar Rachman; Lathifah, Anis Samroatul; Amin, Mohamad; Utomo, Yudhi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10430

Abstract

The native bacteria have a wide life habitat and have an important role as agents in the bioremediation process of waste. Batik as one of the products of Indonesian character, has become a contributor of liquid waste that can reduce water quality. Besides being rich in dyes, batik waste is also rich in fat content. The fat content in batik waste comes from the processing (coating the fabric with a layer of wax) and washing is done repeatedly. Fat has properties that are not soluble in water and is an organic material, which if high concentrations can reduce water quality. This study aims to isolate, identify, and measure the ability of indigenous bacteria to reduce fat content in liquid waste in vitro. The method used is experimental research. Isolation and culture using Busnall Hass media enriched with 1% olive oil, then proceed with a series of biochemical tests for the purpose of characterizing indigenous bacterial isolates. The results showed that Bacillus subtilis and Pseudomonas fluorescens have the potential to reduce the fat content of batik liquid waste.
MEDIA ALTERNATIF CAMPURAN DAUN PISANG KERING DAN KULIT JAGUNG UNTUK MENINGKATKAN PRODUKTIVITAS JAMUR MERANG (VOLVARIELLA VOLVACEA (BULL) SINGER.) DALAM KERANJANG Suparti, Suparti; Safitri, Wardani Ana
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10435

Abstract

The growth of mushroom requires nutrients such as cellulose, hemicellulose and lignin which can be obtained from rice straw. However, the availability of rice straw at certain times is difficult to obtain. Dried banana leaves and corn husk have the potential to be used as alternative growing media because they contain cellulose, hemicellulose and lignin for the growth of mushrooms. This research was conducted to determine the productivity of straw mushrooms on a mixture of dried banana leaf media and corn husk planted in a basked. The method used RAL (Completely Randomized Design) which consisted of one factor, namely a mixture of 500gr, 375gr, 250gr, 125gr, and 0gr corn shells with Ogr dried banana leaves, 125gr, 250gr, 375gr, and 500gr. Data were tested by one-way Anova analysis. The result showed that there was no effect of the mixture of dried banana leaves and corn husk on the productivity of straw mushrroms. Mushroom can grow on all treatments.
BIOMASSA DI ATAS TANAH DAN PENGHITUNGAN SIMPANAN KARBON HUTAN KALIBIRU KABUPATEN KULON PROGO Arsalan, Andy; Gravitiani, Evi; Irianto, Heru
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10426

Abstract

Above Ground Biomass (AGB) forest ecosystem is one of the fundamental parameters to describe each of its functions. Carbon in the forest ecosystem accumulates through absorption of CO2 in the atmosphere and is assimilated into biomass. The study of forest vegetation biomass is important for determining carbon storage in dominant tree components and calculating carbon cycles at regional and global levels. Kalibiru Forest carbon storage information is not yet available, so it is necessary to calculate carbon potential. This study aims to calculate aboveground biomass and estimate carbon stocks in the Kalibiru Forest. The study was conducted in Kalibiru Hamlet, Hargowilis Village, Kokap District, Kulon Progo Regency. The research conducted the indirect method (non-destructed) to calculate biomass and carbon by using the allometric equation. Base on the calculation, Kalibiru Forest has 5.086,52 tons of biomass or 175,40 tons per hectare in average. The potential for carbon storage in the Kalibiru Forest is 2.543,26 tons of carbon or 87,7 tons per hectare in average. This figure means that preserving 1 hectare of Kalibiru Forest can avoid the impact of atmospheric damage due to the greenhouse gas effect as many as 87,70 tons per year. Results of this study provide an insight for the surrounding community about the importance of the existence of the Kalibiru Forest as an absorber of CO2 gas in the atmosphere. As another benefit, it provides motivation for the surrounding community to continue in preserving the forest.
KUALITAS BIOPLASTIK DARI UMBI SINGKONG KARET DENGAN PENAMBAHAN KOMBINASI PLASTICIZER GLISEROL DENGAN SORBITOL DAN KITOSAN Asngad, Aminah; Marudin, Ervian Jan; Cahyo, Devi Setyaning
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10431

Abstract

High plastic demand has the potential to cause problems because plastic is a synthetic polymer made from petroleum that is difficult to decipher. Therefore, saving the current development of biodegradable plastics that can be decomposed by microorganisms naturally. Cassava rubber tubers are rarely used because they contain HCN toxin but have a carbohydrate content of 98.4674%, so it has the potential as a bio-plastic material. The purpose of this study was to determine the Bioplastic Quality of Cassava Rubber with the addition of a combination of Plasticizer Glycerol with Sorbitol and Chitosan. The study was conducted in the lab. Biology FKIP UMS, research methods used are experimental research methods. The study design used was completely randomized design with 2 treatment factors, namely: treatment factor 1 volume of glycerol (G), G 1 = 3ml; G 2 = 5ml; G 3 = 7ml. Treatment factors are 2 types of Plasticizer (P), P1 = Sorbitol and; P2 = Chitosan. The analysis used is descriptive qualitative used to test tensile resistance, elongation at break. Based on the results of the study showed the strongest spacer resistance, G1P1 which is 17.20N / mm², and the highest breaking length, G3P1 which is 24.00%. with sorbitol and chitosan.
VARIASI PERTUMBUHAN DAN PEMBUAHAN KLON JATI (TECTONA GRANDIS L.F.) UMUR 11 TAHUN Baskorowati, Liliana; Adinugraha, Hamdan Adma; Mashudi, Mashudi
Bioeksperimen: Jurnal Penelitian Biologi Vol 6, No 1: Maret 2020
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23917/bioeksperimen.v6i1.10427

Abstract

The development of teak clones (Tectona grandis L.f.) is currently being done by industry and farmers to increase productivity. The use of superior teak clones will determine the success of planting; therefore, the superior clones need to be developed. Knowledge of variations in growth and fruiting in the seed orchard of teak clones is important to determine the reproductive capacity of a plant. Therefore, study on variations in the growth and fruiting of teak clones was undertaken. The study was conducted at 11 years old of teak clones, located in Alas Ketu Wonogiri, Central Java. The teak plants measured were planted with a spacing of 2 x 6 m, using a randomized block design, with 100 clones; 3 ramet per clone and consists of 5 blocks as replications. The clones originated from Gunung Kidul, Madiun, Cepu, Wonogiri, Randublatung, Rembang, Muna, Matakidi, Kendari, Buton, and Thailand. All individual tress in the plots were examine for collecting data. Parameters were measured including height of trees, diameter breast height, flower and fruit production. The results showed that height and diameter growth varied significantly between clones, as well as between replications; while flower and fruit production did not show significant differences between clones and between replications. When the clones grouped in height class and diameter class, it showed that the production of flowers and fruits of teak clones were differs significantly. Moreover, the reproductive success of this teak clone seed orchard was very low at 2.15%, suggested due to very little rainfall during the flowering season which causes many flowers to fall out.