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Majalah Obat Tradisional
Published by Universitas Gadjah Mada
ISSN : 14105918     EISSN : 24069086     DOI : -
Core Subject : Health,
Health Professions Medicine & Pharmacology Public Health
raditional Medicine Journal (Majalah Obat Tradisional), or Trad. Med. J. (ISSN 1410-5918 (print) and ISSN 2406-9086 (online)), is an international scientific journal published by Faculty of Pharmacy, Universitas Gadjah Mada, three times annually. Collaborating with Indonesian Pharmacist Association, Daerah Istimewa Yogyakarta, and we dedicate our journal to researches and development in traditional medicine. The journal receives papers on research laboratory, field research, and case studies of traditional medicine and its constituent, covering research topics including raw materials, cultivation, phytochemical, pharmacological effects and toxicology, formulation, and biotechnology.
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Articles 564 Documents
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ISOLASI DAN IDENTIFIKASI SENYAWA ANTIBAKTERI DARI DAUN PETAI CINA (Leucaena leucocephala (Lam.) De Wit.) Ari Sartinah; Puji Astuti; Subagus Wahyuono
Majalah Obat Tradisional Vol 15, No 3 (2010)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (541.242 KB) | DOI: 10.22146/tradmedj.8138

Abstract

Plants are important natural resources widely explored for maintaining health and treatment of diseases. One of them is Leucaena leucocephala leaf which is used as traditional medicine. Indonesian has used this leaf to treat wound and inflammation. This study was conducted to isolate and identify antibacterial compounds from L. leucocephala leaves. Dry powder of this leaf was extracted using washbenzen by Soxhletation method followed by methanol extraction. Both extracts were tested for antibacterial activity against Staphylococcus aureus ATCC 25923 dan E. coli 25922 and analysed by TLC. Positive extract to S. aureus was further separated by vacuum liquid chromatography using different combination of washbenzene and ethyl acetate followed by analysis using TLC. Each fraction was tested for antibacterial activity and analysed by TLC. The positive fraction was further separated by TLC-preparative, assigned as p1a, p2a, p3a. The active compound (p2a) was tested for its purity using TLC with various mobile phase. Its structure was identified using combination data of UV-Vis and IR spectrophotometry, GC-MS and NMR.  Based on UV-Vis data, this compound showed the absence of chromophoric group.  IR data showed adsorption at 3409,4 cm-1 (OH), 2928,2 cm-1(CHaliphatic), 2854,3 cm-1(CHaliphatic), 1575,5 cm-1 (C=C), 1416,4 cm-1 (CH2), 1385,0 cm-1(CH3), 1258,3 cm-1 and 1082,3 cm-1 (C-O). GC-MS data showed molecule ion at m/z 482   (M + H+)and fragmented ion at  m/z 427   (M + H+). 1H-NMR (CDCl3) spectrum showed resonance at δ 0,8; 1,4; 1,6; 2,0; 2,3; 3,6; 4,2 and  5,4 pp, suggesting that this compound is lupeol. 
Prediction of Geraniol Bond Mode in Aspergillus niger Linalool Dehydratase – Isomerase Yelfi Anwar; Andrianopsyah Mas Jaya Putra; Elvina Dhiaul Iftitah; Partomuan Simanjuntak; Shirly Kumala
Majalah Obat Tradisional Vol 24, No 2 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (629.33 KB) | DOI: 10.22146/mot.45599

Abstract

Geraniol is a very valuable aroma chemical and has commonly been used in fragrances and aroma compound. Geraniol biotransformation by Aspergillus niger has been studied. The main bioconversion products obtained from geraniol and liquid culture of A. niger are linalool and alpha-terpineol. Linalool plays a major role in anti-inflammatory, antibacterial and antioxidant activities. This study aims to know the interaction of geraniol in Aspergillus niger enzyme with docking molecular. Comparative modeling of Aspergillus niger enzyme was conducted by means of one of the crystal structure of Linalool Dehydratase – Idomerase (LDI) as a template. The best model of this comparative modeling was then used in docking molecular to investigate geraniol binding mode inactive site enzyme of Aspergillus niger. Inactive site enzyme of Aspergillus niger, geraniol is located with hydrophobic and hydrogen bonds: Amino acid – the amino acids are Asn 105, Arg 96, Lys 112 inactive site - OH with hydrogen bond, Arg 97 inactive site – CH3 with hydrophobic bond and Leu54 inactive site – CH3 with the hydrophobic bond. The distances among pharmacophore respectively are 3,603 A, 6,768 A, and 7,345A. It has higher score (ΔGbind: -3.4 kcal/mol) compared to linalool (ΔGbind: -3.6 kcal/mol). Virtual tethering of linalool with LDI Aspergillus niger enzyme in amino acid Leu120 and Glu118 had been done. The pharmacophore is - OH and methyl C8 group. The distances among pharmacophore respectively are 5,835 Å, 2,52 Å, and 5,32 Å. Virtual tethering of LDI Aspergillus niger enzyme with geraniol has a higher score (ΔGbind: -3.4 kcal/mol) compared to linalool (ΔGbind: -3.6 kcal/mol). It shows that interaction between linalool and LDI Aspergillus niger enzyme is easier to occur than the interaction between geraniol and LDI Aspergillus niger enzyme, geraniol reaction to linalool that occurs is rearrangement reaction.
Metabolite Fingerprinting Eleutherine palmifolia (L.) Merr. Using UPLC-QTOF-MS/MS Roihatul Mutiah; Weka Sidha Bhagawan; Burhan Ma'arif; Jauhar Maknun Septaza Rahmandika
Majalah Obat Tradisional Vol 24, No 3 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.44883

Abstract

Eleutherine palmifolia (L.) Merr. (E. palmifolia) is an Indonesian native plant that has the potential to be developed into phytopharmaca. The differences in growth locations are thought to cause variation in the content of metabolite compounds which affect differences in pharmacological activity. This study aims to determine the profile of metabolites E. palmifolia bulb from several regions in Indonesia. The samples were collected from six different locations, namely East Java, Central Java, West Java, East Borneo, Central Borneo, and South Borneo. Sample extraction was carried out using Ultrasonic Assisted Extraction (UAE) method with 96% ethanol. The analysis of the content of metabolites was carried out using UPLC-QTOF-MS/MS with a stationary phase column C18 (Okta Decyl Silica), mobile phase mixture of formic acid /water 0.1/99.9 (v/v), and formic acid/acetonitrile 0,1/99,9 (v/v). The results of the analysis were interpreted using software Masslynx and continued with chemometric analysis using the method Principle Component Analysis (PCA). The results showed that there were differences in the content of the metabolite compounds in E. palmifolia bulb originating from six different regions.
Ethanol Extract of Detam 1 Soybean Seed (Glycine Max L. Merr) for Chronic Kidney Disease Therapy by In Vitro Study Sijani Prahastuti; Meilinah Hidayat; Stella Tinia Hasiana; Wahyu Widowati; Annisa Amalia; Rismawati Laila Qodariah; Rizal Rizal; Satrio Haryo Benowo Wibowo; Hanna Sari Widya Kusuma
Majalah Obat Tradisional Vol 24, No 3 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.45053

Abstract

Chronic Kidney Disease (CKD) has increased incidence and prevalence in developing nations. In this in vitro study, we evaluated the cells proliferative effects, fibronectin (FN), transforming growth factor β (TGF-β1), and Reactive oxygen species (ROS) - level inhibition potential of ethanol extract of detam 1 soybean seed (EEDS) on glucose-induced kidney mesangial cells (SV40 MES 13). The cells proliferation assay used 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)2H-tetrazolium (MTS) assay. FN and TGF-β1 level were measured using ELISA assay kit and ROS level using flow cytometry. Level of   FN, TGF-β1 and ROS, on CKD cells model (5 mM, 10 mM glucose-induced mesangial cell) treated with EEDS 6.25 µg/mL were lower significantly compared to positive control,  EEDS improve cells viability and decrease FN, TGF-β1 and ROS level in glucose-induced kidney mesangial cells as CKD cells model.
The Influence of Channa Striata Extract Emulgel on Incision Wound Healing in White Rats Lucia Hendriati; Suryo Kuncorojakti; Teguh Widodo; Heny Kristi Meitasari; Wilis Prasasti
Majalah Obat Tradisional Vol 24, No 3 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.45080

Abstract

Snakehead fish (Channa striata) have high albumin content, a protein needed for cell development and the formation of new tissue. The aim of this study was to determine the influence of snakehead fish extract emulgel given topically on incision wounds in white rats. The parameters of wound healing consist of wound length, a number of neutrophils, macrophages, fibroblasts and density of collagen. The white rats divide into three groups of (n = 6), one group was given the emulgel base as the negative control, one group of povidone-iodine as the positive control, and one group of snakehead fish extract 10% emulgel. White rats were sacrificed on the third and seventh days for microscopic observations. The results showed that snakehead fish extract emulgel can accelerate incision wound healing: decrease wound length, increase the number of neutrophil and macrophages cells, increase the average number of fibroblasts and increase collagen density on white rats.
The Differences of Malondialdehyde Serum Level, Expression of Tumor Necrosis Factor Alpha and Vascular Endothelial Growth Factor, and the Area of Endometriotic Implants in Administration of Kebar Grass Extract (Biophytum petersianum) and Green Tea Extract (Camelia sinensis) to Mice Yuli Trisetiyono; Noor Pramono; Syarief Thaufik Hidayat; Widjiati Widjiati
Majalah Obat Tradisional Vol 24, No 3 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.45237

Abstract

The pathological pathway of endometriosis remains unclear and involves complex etiologies. Increased oxidative stress is understood to be related to this disease. Oxidative stress produces reactive oxygen species, causes inflammation that is characterized by recruiting lymphocytes and phagocyte activation, produces cytokines that induce oxidation enzyme, and supports epithelium growth. Oxidative stress conjointly will increase angiogenesis and promotes the proliferation of endometriosis tissue within the peritoneal cavity. Kebar grass and green tea contain high antioxidants, are expected to extend antioxidant defense leading to reduced oxidative stress, inflammation, angiogenesis, and reduced endometriosis tissue implants. The objective is to analyze the consequences of Kebar grass and green tea extract to MDA serum level, TNF-α, and VEGF expression, and the area of the endometriotic implants in the mice models.  The study was an experiment designed. It has been conducted within the Department of Obstetrics Gynecology, Faculty of Medicine Diponegoro University and Faculty of Veterinary Medicine Airlangga University. Twenty-one mice were divided into three groups, i.e., the first group of mouse models was given Kebar extract 3 mg/day; the second group was assigned green tea extract 1.1 mg/day; therefore the third group was a control group contains the untreated endometriosis mice. Each treatment was given for fourteen days. MDA serum level was measured by specto-photometric examination, the area of the endometriotic implants was measured by computer tracing technique, whereas TNF-α and VEGF expression of endometriotic implants were measured by IHC using Rammele Scale Index (ImmunoReactive Score). The MDA serum levels of the groups treated with Kebar grass extract and green tea extract were significantly lower than the control group (0.09±0.022 mmol, 0.07±0.019 mmol, and 0.30±0.062 mmol, respectively; p=0.001). TNF-α expression of the groups supplied with each treatment also lower than the control groups (2.43±1.521, 3.66±1.422, and 7.26±2.898, respectively; p=0.002). However, VEGF expression was not significantly different between Kebar grass extract group, green tea group, and the control (4.34±2.402, 4.57±1.998, 7.40±3.495, respectively; p=0.089). Finally, the area of the endometriotic implants of the mice models administered with all treatment was smaller than the control group (0.01±0.025 mm2, 8.76±18.776 mm2, and 34.80±13.079 mm2, respectively; p=0.003). Conclusion: Kebar grass extract, as well as green tea extract administration to endometriosis model mice, resulted in lower MDA serum level and TNF-α expression, smaller the area of endometriotic implants compared, but not resulted in a significant difference of VEGF expression.
A Review of Component and Pharmacology Activities of Black Garlic Novitaria Br Sembiring; Yoppi Iskandar
Majalah Obat Tradisional Vol 24, No 3 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.45277

Abstract

Black garlic is garlic fermented in high temperature and humidity, and the fermentation compound chemical in garlic changes to new chemicals, such as S-allyl cysteine (SAC), S-allylmercapto-cysteine, and arginine. The color changed to dark brown during the aging period because of the Maillard reaction, known as non-enzymatic browning reaction. Black garlic has 57.14% of fructose, 6.78% of glucose, and 7.62% of sucrose, which were mainly saccharides in black garlic to answer for its sweet taste. It is such a pharmacological activity which contains antioxidant, anti-cancer, hepatoprotective, can improve the immune system, reduce hyperglycemia, dyslipidemia, and anti-allergic. We summarize the current knowledge of a change in the components, bioactivity, production, and applications of black garlic, as well as the proposed future prospects on their possible applications as a functional pharmacy product.
Antibacterial Activity of Cuttlefish Sepia sp. (Cephalopoda,) Ink Extract Against Aeromonas hydrophila R Adharyan Islamy
Majalah Obat Tradisional Vol 24, No 3 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.45315

Abstract

Cephalopods ink has shown potential antiretroviral activity. The ink extracts of cuttlefish showed an antibacterial effect. This study aims to investigate the antibacterial activity of the methanolic extract of the ink of cuttlefish Sepia sp. against Aeromonas hydrophila. A. hydrophila are opportunists and associated with aquatic fish and shrimp disease. The shade-dried ink sample from approximately 30g ink sacs obtained from 15 animals was immersed separately in methanol (1:3 w/v) solvents for overnight. The experiment in this study used the dried extract of cuttlefish ink. The isolate of A. hydrophila was originated from Jepara Brackishwater Aquaculture Center. The average yield percentage of cuttlefish ink extract obtained was 4.86%. The results of the MIC test in Table V show that the highest average absorbance value obtained was at a concentration of 50 ppm, which was equal to 1.716 nm, and the lowest absorbance was obtained at a treatment dose of 300 ppm at 0.841 nm, while the Mc Farland tube was 0.933 nm. The results of antibacterial test on Table II showed antibacterial activity of cuttlefish ink extract at negative concentration control showed diameter zone of 5 ± 1.2 mm, at positive control showed diameter zone of 31 ± 1.2 mm, at 250 ppm result 19 ± 0.9 mm, at 300 ppm result 22 ± 1.4 mm, at 350 ppm result 31 ± 1.2 mm.
Assessment of Self-Nanoemulsifying Drug Delivery System (SNEDDS) of Ethyl Acetate Fraction from Mangosteen (Garcinia mangostana L.,) Peels to Eschericia coli, Pseudomonas aeroginosa, and Proteus mirabilis Liza Pratiwi
Majalah Obat Tradisional Vol 24, No 3 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.45409

Abstract

Mangosteen peels have antibacterial activity. SNEDDS has many advantages in developing a drug delivery system to increase the penetration of active compounds. The purpose of this study was to compare the effectiveness of the antibacterial SNEDDS of ethyl acetate fraction from mangosteen peels and ethyl acetate fraction of mangosteen peels without SNEDDS preparation as an antibacterial against Eschericia coli, Pseudomonas aeroginosa, and Proteus mirabilis, that cause diabetic ulcers. This research began with maceration. The thick ethanol extracts were continued and fractionation was carried out with ethyl acetate solvents then was formulated into SNEDDS. The measurement of the antibacterial activity with the bacterial growth inhibition parameters of SNEDDS preparations extracted from ethyl acetate fraction of mangosteen peels was compared with ethyl acetate fraction of mangosteen peels without SNEDDS preparation. Data were analyzed using independent sample T-Test. The results showed the SNEDDS preparation of ethyl acetate fraction from mangosteen peel had activity against both types of bacteria causing diabetic ulcers, but it had no activity against Proteus mirabilis. The results of statistical analysis showed that there were significant differences in the activity of SNEDDS ethyl acetate fraction of mangosteen peels and ethyl acetate fraction of mangosteen peels without SNEDDS in Eschericia coli and Pseudomonas aeruginosa.
Hepatoprotective Effect of Citrus Sinensis Peel Extract Against Isoniazid and Rifampicin-induced Liver Injury in Wistar Rats Edward Kosasih; Linda Chiuman; I Nyoman Ehrich Lister; Edy Fachrial
Majalah Obat Tradisional Vol 24, No 3 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.45762

Abstract

Tuberculosis (TB) is one of the leading causes of death in developing countries. One of the problems in controlling TB disease is that most anti-tuberculosis drugs are hepatotoxic. Citrus sinensis peel extract is the rich source of secondary metabolites with high potential effectiveness as an antioxidant. In the present study, we evaluate the hepatoprotective effect of Citrus sinensis peel ethanolic extract (CSPEE) on isoniazid and rifampicin-induced liver injury in Wistar rats. Twenty five adult male Wistar rats were divided into 5 groups of 5 each : control,  (INH+RIF) (50 mg/kg bw once a day for 14 days), (INH+RIF) + various dose of CSPEE (300, 450, 600 mg/kg bw).  CSPEE was given orally once a day for 14 days followed by administration of INH + RIF suspension. The measurement of serum ALT and AST were carried out on the 15th day. Histopathologic examination of the liver was also performed. The Serum ALT and AST of the rats that induced with INH + RIF were increased significantly (P<0.001) compare to those of control groups, and the histopathologic slides showed steatosis, vacuolation and necrosis of hepatic cells. The serum ALT and AST in groups treated with CSPEE were not significantly different (p>0.05) with those of control groups. The serum ALT and AST and histopathological examination of the liver of the group that administered 600 mg/kg CSPEE were closest to normal rats. Citrus sinensis peel extract exhibits hepatoprotective effect on liver injury induced with INH + RIF in Wistar rats.

Page 37 of 57 | Total Record : 564

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