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METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 316 Documents
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UJI AKTIVITAS EKSTRAK BIJI KAPUK RANDU (Ceiba pentandra Gaertn.) TERHADAP PERILAKU KAWIN TIKUS (Rattus norvegicus) JANTAN Dessy Sukma Wirastuti; Ngurah Intan Wiratmini; Sang Ketut Sudirga
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p02

Abstract

Kapuk randu (Ceiba pentandra Gaertn.) merupakan salah satu tanaman yang memiliki banyak manfaat untuk masyarakat. Tanaman ini dimanfaatkan sebagai penahan tanah dari erosi, tanaman peneduh, bahan pengisi kasur dan pakan ternak. Saat ini biji kapuk randu banyak dikembangkan sebagai bahan obat kontrasepsi herbal. Tujuan penelitian ini adalah untuk mengetahui aktivitas ekstrak biji kapuk randu (Ceiba pentandra Gaertn.) terhadap perilaku kawin tikus (Rattus norvegicus) jantan. Rancangan yang digunakan adalah Rancangan Acak Lengkap (RAL) dengan 4 kelompok perlakuan dan 8 ulangan yaitu P0 (aquades dan tween 80), P1 (0,07 mg/kg BB ekstrak biji kapuk randu), P2 (0,12 mg/kg BB ekstrak biji kapuk randu), P3(0,24 mg/kg BB ekstrak biji kapuk randu). Pemberian perlakuan ekstrak biji kapuk dilakukan selama 33 hari dengan pengamatan perilaku kawin dilakukan 3 hari sekali. Parameter yang diamati untuk menilai adanya perilaku kawin adalah jumlah kissing vagina, jumlah mount jumlah intromission. Hasil penelitian menunjukkan bahwa ekstrak biji kapuk randu memberikan pengaruh yang nyata terhadap perilaku kawin tikus jantan yang ditunjukkan dengan menurunnya jumlah kissing vagina, jumlah mount dan jumlah intromission tikus jantan kelompok perlakuan.
Kemampuan Jamur Akar Putih (Rigidoporus microporus (Swartz: Fr.) Van Ov.) dalam Mendegradasi Limbah Selulosa Dianty Putri; Nasril Nasir; Anthoni Agustien
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p16

Abstract

Cellulose is one of abundance natural biopolymer with limited usage. The common found waste cellulose are waste straw, sawdust, and pineapple pit. Rigidoporus microporus is a white root fungi groups that often attack the roots of plants that rot and die. This study was conducted to assess the ability of white root fungi (R. microporus) to degrade waste cellulose, according to cellulolitic activity index of potential R. microporus isolate. The medium used was Carboxy Methyl Cellulose (CMC) with dye Congo Red (0,1%). The results obtained that R. microporus has cellulotic index 3,23 which indicated as potential cellulose degrade.
Pengaruh Pemberian Pakan Beberapa Alga Makro (Ulva sp., Gracilaria sp., Halymenia sp.) Terhadap Pertumbuhan Abalon Haliotis squamata Desi Damayanti; Deny Suhernawan Yusup; Ibnu Rusdi
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p08

Abstract

Teknologi produksi benih dan pakan abalon Haliotis squamata telah berhasil dikembangankan oleh Balai Besar Penelitian dan Pengembangan Budidaya Laut, Gondol, sedangkan pakan untuk pendederan juvenil abalon masih memerlukan pengembangan lebih lanjut untuk memperoleh bahan pakan yang optimum untuk pertumbuhan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian pakan beberapa jenis alga makro segar (Ulva sp., Gracilaria sp., Halymenia sp.) yang diberikan dalam komposisi tunggal ataupun kombinasi (7 komposisi) terhadap pertumbuhan juvenil abalon H. squamata. Rancangan penelitian yang digunakan yaitu menggunakan Rancangan Acak Lengkap (RAL) 3 kali ulangan. Percobaan dilakukan dalam keranjang plastik ukuran 287 x 140 x 52 mm dengan kepadatan 25 ekor (berat badan 1,75 ± 0,08 g, panjang cangkang 21,96 ± 0,34 mm, lebar cangkang 13,37 ± 0,24 mm). Pengukuran abalon dilakukan setiap 2 minggu sekali, dan pemeliharaan abalon dilakukan selama 87 hari dengan pemberian pakan setiap 2 hari sekali. Hasil penelitian ini diketahui bahwa perlakuan dengan pemberian pakan kombinasi Ulva sp., Gracilaria sp., dan Halymenia sp. memberikan pertumbuhan yang paling baik pada berat badan (BB), panjang cangkang (PC), serta lebar cangkang (LC), berturut-turut yaitu 4.70±0.20 g, 30.61±0.44 mm, serta 18.94±0.26 mm, sedangkan perlakuan pakan Halymenia sp. yang terendah. Nilai rasio konversi pakan (FCR) terendah yaitu pada perlakuan Ulva sp. dan Halymenia sp. 13,55±1,06, dan tertinggi yaitu pada perlakuan Gracilaria sp. 23,89±2,63.
Karakter Morfologi dan Analisis Daerah Conserved Gen Elongation Factor 1? (EF1?) pada Lepidotrigona terminata Suprianto Suprianto; Manap Trianto; Nur Alam; Ni Gusti Ayu Galuh Candra Kirana
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p05

Abstract

Lepidotrigona terminata is one of the stingless bees of the Apidae family. Morphological characteristics of L. terminata species are very rare due to their genetic characteristics. The elongation factor 1? gene has been studied in the growth and development of both morphology and molecular systematics. This study aims to describe the morphological character of L. terminata from Temanggung Regency, Central Java with a purposive sampling method and analyze the conserved gene elongation factor 1? area to explain genetic variation based on the proportion of DNA bases analyzed in silico and computational methods using a database in GenBank with number access DQ813123.1, DQ813125.1 and DQ813124.1. The results of the analysis explained that the morphological character of L. terminata is very difficult to distinguish from several other Lepidotrigona groups and it is suspected that the genetic trait of the elongation factor 1? gene has an influence on the morphological character of L. terminata. The proportion of DNA bases of the target gene has a length of about 682 bp to 780 bp with the highest proportion of bases found in Adenine (A) around 216 bp (31.67%) to 251 bp (32.18%), the least number of bases found in Guanin (G) around 121 bp (17.74%) to 140 bp (17.95%) and has twelve conserved gene regions. The data obtained from the research provide basic information to study the morphological characteristics and conserved regions of the elongation factor 1? gene in L. terminata. keywords: L. terminata, morphology, elongation factor 1?, in silico, computational methods
Konservasi Air Sebagai Program Green Hotel Pada Hotel Melati Di Kawasan Pariwisata Sanur; Bali Stephanie Felitania Lestari; A.A.G. Raka Dalem; I K Sundra
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p14

Abstract

Sanur merupakan satu dari lima Sustainable Toursim Observatory (STO) dari UNWTO yang terdapat di seluruh Indonesia. Sebagai kawasan pariwisata tertua di Bali dan juga satu-satunya kawasan pariwisata di Kota Denpasar, Kawasan Pariwisata Sanur (KPS) tidak dapat terhindar dari berbagai permasalahan. Hotel melati yang terdapat pada kawasan ini juga menghadapi permasalahan yang sama, salah satunya adalah permasalahan air. Menanggapi hal tersebut, Kementrian Pariwisata Republik Indonesia menghimbau seluruh hotel untuk mengembangkan pariwisata yang ramah lingkungan melalui audit lingkungan yang berdasar pada prinsip-prinsip Green Hotel. Hal tersebut dilakukan agar hotel melakukan berbagai perbaikan untuk mewujudkan pariwisata berkelanjutan. Penelitian ini dilakukan untuk mengetahui kinerja implementasi Green Hotel aspek konservasi air pada manajemen hotel melati di KPS. Pengambilan data dilakukan pada bulan Agustus hingga Desember 2018 melalui observasi, wawancara, penyebaran kuesioner, studi literatur, dan pemeriksaan dokumen. Data dianalisis secara deskriptif kualitatif dan kuantitatif. Hasil penelitian menunjukan bahwa penerapan konservasi air di KPS hanya dilakukan dengan baik oleh 23,077% hotel, sementara itu sebanyak 76,923% belum menerapkannya dengan baik.
Cover & ToC Admin Metamorfosa
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

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Monitoring Proviral DNA Of Jembrana Disease Virus In Bali Cattle Using PCR Ni Putu Eka Krisnayanti; Made Pharmawati; Inna Narayani; Ni Luh Putu Agustini
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p03

Abstract

The development of biotechnology in molecular field make it possible to detect the presence of a disease earlier, faster and more accurately. One of the technique in biotechnology which has been highly developed and often be used in biological and medical research is PCR method. This molecular technique allows to accurately identify the DNA level in presence of a particular disease. This study aims to do monitoring the presence of proviral DNA Jembrana Disease Virus in bali cattle from all regencies in Bali. The blood samples of bali cattle is taken from all regencies in Bali in 2017 as many 170 samples and in 2018 from Buleleng Regency there are 101 samples and form Karangasem Regency 152 samples. DNA is extracted by QIAamp DNA blood mini kit and samples from 2017 is made to 34 pool samples, whereas samples from 2018 is made to 20 pool samples (Buleleng Regency) and 30 pool samples (Karangasem Regency). PCR is conducted by JDV 1 primer and JDV 3 primer in total reaction 25 µL with 35 cycles. PCR is also conducted on positive control and negative control. The result indicate that 34 pool samples of bali cattle from 2017 or 20 pool samples and 30 pool samples from 2018 is not indicate the presence of proviral DNA Jembrana Disease Virus. Keyword: PCR, proviral DNA, Jembrana Disease Virus
Induksi Perkecambahan In Vitro Jeruk Hasil Fusi Protoplas Pada Medium Dengan Penambahan Asam Giberelat A A Ayu Laksmi Damarnegari; Ida Ayu Astarini; Made Ria Defiani; Mia Kosmiatin
Metamorfosa: Journal of Biological Sciences Vol 7 No 2 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i02.p10

Abstract

Plant breeding is an effort to develop plants by manipulating the genetic system of plants to get new superior cultivars. Plant breeding often produces incompatible seeds, so a rescue process is needed to reduce the risk of embryo death. The addition of gibberellic acid could break the seed dormancy for seed that hard to germinate. The purpose of this study was to find out the best concentration of gibberellic acid for germination of citrus globular embryos from protoplast fusion. Research was conducted at the Biology and Cell Biology Research Group In Vitro Culture Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development (BB BIOGEN), Agricultural Research and Development Agency, Ministry of Agriculture, Bogor. The study used Completely Randomized Design (CRD) with concentration 0, 1, 2 and 3 ppm of gibberellic acid as treatment, each consists of 7 replicates. This study used globular embryos measuring ± 3 mm of inbred FS 29 and FS 89 from protoplast fusion between Siam Simadu and Mandarin Satsuma citrus. Observations were made on the percentage of germinated embryos, time of germination, the number of the complete and incomplete shoot (abnormal, only part of the roots or buds that developed). Data were analyzed using Crosstab analysis test with IBM SPSS Statistics 22 software. Result showed that addition of gibberellic acid induce faster germination time and increase seedling percentage. The best germination medium for citrus FS 29 was VMW with 2 ppm and 3 ppm gibberellic acid with the seedling percentage around 85,7% in 7 days, while the best germination medium for citrus FS 89 was VMW with 1 ppm gibberellic acid with 100% seedling embryos in 8,5 days. VMW medium added with 1 ppm, 2 ppm and 3 ppm gibberellic acid for citrus FS29 and VMW medium added with 2 ppm and 3 ppm for citrus FS89 were good to get the complete sprouts with a succes rate of 100%. Keyword: Citrus sp. gibberellic acid, incompatible, in vitro, embryo germination
UJI EFEKTIVITAS EKSTRAK ASETON DAUN KAYU MANIS (Cinnamomum burmanni Blume) TERHADAP JAMUR FUSARIUM SOLANI PENYEBAB PENYAKIT BUSUK BATANG PADA BUAH NAGA (HYLOCEREUS SP.) SECARA IN VITRO Anak Agung Ketut Darmadi; I Ketut Ginantra; Martin Joni
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p13

Abstract

This research heads for examining the effectiveness of acetone extract of cinnamon leaf  (Cinnamomum burmanni Blume) to the growth of Fusarium soloni fungus. Cinnamon leaf which was used in this research grows in Bedugul village Tabanan regency Bali province. The extract effectiveness experiment of cinnamon leaf to the fungus experiment  was carried out by well difusion method in Biopesticide Laboratory of Agriculture Faculty Udayana University, by using the complete random plan consists of 6 treatments and 4 times refrains. The concentration treatment of acetone  extract of cinnamon leaf obviously (P<0.05) can impede the growth of colony, biomass and establishment of experiment fungus spore i.e. Fusarium soloni in accordance with in-vitro by PDA and PDB media. Extract minimum blocked energy of cinnamon leaf in experiment fungus i.e. 0,5%. The extract of cinnamon leaf obviously can impedethe growth of colony, spore establishment and biomass establishment of experiment fungus. In extract concentration treatment 0.5% obviously can impede the colony growth of experiment fungus, spore establishment and fungus biomass establishment i.e. each of them in amount of  17,3%, 41,45% and 7,94% if they are compared by the control. The higher extract concentration then the blocked energy is the bigger.
EFFECT OF ENVIRONMENTAL DEGRADATION ON ANIMAL DIVERSITY IN BALI, INDONESSIA I Wayan Kasa
Metamorfosa: Journal of Biological Sciences Vol 2 No 2 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Bali is a small beautiful tropical island of Indonesia archipelago, lies between the continent of Asia and Australia, as well as the Indian and Pacific Ocean. As a tropical archipelago, many kinds of biodiversity can be found. The island of Bali in particular, there are typical animal diversity that could not be investigated beyond such island, such as, Bali cattle, Bali dog, Bali starling and others. As time goes on, the existance of such biodiversity decreases in both body weight and population number. Both global warming/climate change and land use change are the main factors affecting such phenomenon. This study has been conducted by employing field observation as well as literature study. It was found that, the quality of purebred Bali cattle species decreases genetically that could be notified of smaller bodysize for both male and female. Land use change of agriculture activity to the hotels, house of living, roads and other infrastructures are the main factors for Bali cattle existence. For typical famous bird of Balistarling, the problem is because of deforestation which cause natural habitat loss, due to land use change for agricultural activity and house building by local people. In case of Bali dog, the mad dog of rabies is just introduce and spreading over Bali island, whichis formerly the island of Bali has been recognised as free zone area of the rabies. As consequence, suffering dogs must be eliminated by  mass killing cause decrease total number of such poor dog. Overall, it could be concluded that environmental degradations of land use change, deforestation and desease are the main causes of biodiversity decreasing number of the Bali cattle, Bali white starling and Bali dog respectively, beside global warming/climate change natural disaster. Key words: Environment, Bali cattle, Bali starling, Bali dog, Bali island.

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