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METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 316 Documents
Formulasi Serum Ekstrak Buah Malaka (Phyllanthus emblica) Sebagai Anti Aging Rosa Ichi Harum Pratiwi; ni luh arpiwi
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p12

Abstract

Serum merupakan produk cairan sedikit kental yang diaplikasikan pada kulit wajah. Penggunaan serum di kalangan wanita sudah banyak diminati bahkan menjadi salah satu perawatan rutin. Seiring berkembangnya zaman, kini banyak orang yang tidak bertanggungjawab dalam menjual produk serum. Kandungan serum diproduksi dengan bahan kimia yang tidak sesuai dosis atau pun menambahkan bahan aktif yang dapat memicu terjadinya masalah kulit. Beberapa masalah kulit yang timbul yaitu bintik-bintik hitam, garis-garis halus dan penuaan. Mengurangi dampak masalah kulit tersebut, maka dilakukan pembuatan serum berbasis “back to nature” yang mengutamakan bahan herbal sebagai salah satu alternatif formulasi serum yang aman. Salah satu bahan alternatif tersebut yaitu buah malaka (Phyllanthus emblica). Buah malaka dipercaya memiliki kandungan antioksidan yang tinggi sehingga dapat memperlambat penuaan dan dijadikan bahan serum untuk anti aging. Metode yang digunakan dalam penelitian yaitu dengan uji DPPH dan uji fitokimia. Hasil penelitian menunjukkan bahwa ekstrak buah malaka tergolong antioksidan kuat dengan nilai aktivitas antioksidan 143,557%, nilai IC50 sebesar 38,649 ppm, flavonoid sebesar 4,6123%, tanin sebesar 112,21%, vitamin C 27,0625%, aktivitas antioksidan serum F1 0,0008%, F2 0,0121% dan F3 0,0103%. Uji viskositas menunjukkan nilai 15.000 mPars, pH pada serum F1 yaitu 4,79; F2 5,00; F3 5,75. Uji homogenitas serum menunjukkan kehomogenan yang baik dan uji hedonik terhadap 3 sampel serum (F1, F2 dan F3) dengan 30 probandus. Hasil yang paling optimal yaitu serum F3 dengan konsentrasi 1,5 %.
Elusidasi Dan Daya Hambat Ekstrak Etanol Daun Sembung Delan (Sphaeranthus indicus L.) Terhadap Candida albicans ATCC 1023 Yulita Salma Lani; Ida Bagus Gde Darmayasa; Ni Made Susun Parwanayoni
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2021.v08.i02.p18

Abstract

The sembung delan (Sphaeranthus indicus L.) plant is one of the weeds in Indonesia which has great potential as an alternative antifungal. The purpose of this study was to determine the ability of sembung delan leaf extract to inhibit the fungus Candida albicans ATCC 1023, as a pathogenic species and cause of candidiasis, as well as chemical compounds contained in the extract. The research design used in this study was a completely randomized design (CRD) divided into 7 treatments and 4 repetitions with one treatment factor, namely the concentration of 1% (w/v), 2% (w/v), 3% (w/v), 4% (w/v), 5% (w/v). Positive control (Nystatin) and negative control (Solvent). Phytochemical tests were carried out and analysis of compound content using GC-MS. The results showed that sembung delan leaf extract was able to inhibit C. albicans ATCC 1023 with the greatest inhibition, namely at a concentration of 5% (w/v) with a diameter of 13.00 mm, a minimum concentration of 0.3% (w/v) with a diameter of 5.925 mm. Statistically significantly different (P?0.05) with a concentration of 0.1% (w / v), and 0.2% (w / v) with a diameter of 0.000 mm and an optimum concentration of 4% (w/v) with a diameter of 11.25 mm. Phytochemical test results of the crude extract of sembung delan contain saponins, phenols, steroids, terpenoids, alkaloids, and tannins. For GC-MS analysis, 7 active compounds were obtained with 6 of them known as anti-fungal compounds, namely Thymol; 1-Tetradecanamine, N, N-dimethyl-; Benzene, 2-methoxy-1,2,3-trimethyl-; 6-Octenal, 3,7-dimethyl-; Thymol and Benzyl chloride. The active compounds contained in sembung delan leaf extract have the opportunity to be used as herbal ingredients to control C. albicans ATCC 1023. Keywords: Minimum Inhibitory Concentration, phytochemicals, thin layer chromatography, gas chromatography mass spectrometry.
Indeks Ekologi dan Status Konservasi Jenis Burung (Avifauna) di Desa Gunaksa dan Beberapa Wilayah di Sekitarnya di Kabupaten Klungkung, Bali I Made Sara Wijana; I Made Saka Wijaya; M. Rheza Rizki Syahputra; Gde Oka Widiyavedanta; Egmont congdenjit; Gede Surya Indrawan; Abd. Rahman As-syakur; Putu Angga Wiradana
Metamorfosa: Journal of Biological Sciences Vol 8 No 2 (2021)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v08.i02.p07

Abstract

The Gunaksa Village and several surrounding areas in Klungkung Regency, Bali have the potential to be developed as one of the international ecotourism destinations in Indonesia. This study aims to determine the diversity and status of bird species (avifauna) in the Gunaksa Village Area and several surrounding areas so that it is expected to be the basis for information on natural resource management in this area. The method used is the "Cruise method", which is tracing paths that can be used to represent various types of ecosystems in the research area. The results showed that 46 species of birds were obtained with the highest Relative Abundance Index (RAI) value obtained by the buffalo egret (Bubulcus ibis) of 9.50% (dominant). The ecological index of bird species shows a high category in the Diversity Index (H') (3.16), Richness Index (R) (7.15), and Evenness Index (E) (1.87). Species of the black-winged kite, Javan ermine, Christmas frigatebird, and Pied fantail are protected species based on the Regulation of the Minister of Environment and Forestry of the Republic of Indonesia Number p.106/menlhk/setjen/kum.1/12/2018, while only the frigatebird Christmas is included in the IUCN RedList with the Critical (CR) category. Conservation efforts and the participation of government agencies, academics, practitioners and the community are needed to increase the environmental awareness of the Gunaksa area and its surroundings for the sustainability of Avifauna in the area.
Deskripsi Perilaku dan Status Darah Burung Hantu Celepuk Jawa (Otus angelinae) dengan Pemberian Suplemen Serbuk Kunyit di Penangkaran Tyas Rini Saraswati; Enny Yusuf Wachidah Yuniwarti; Silvana Tana
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p01

Abstract

Studi perilaku dan analisis status darah celepuk jawa (Otus angelinae) dengan pemberian serbuk kunyit merupakan langkah awal dalam usaha penangkaran. Empat ekor Otus angelinae betina dan 4 ekor jantan dengan bobot 9 g. Otus angelinae, masing-masing dibagi dalam 4 kelompok, yaitu kelompok K0: Otus angelinae betina kontrol, K1: : Otus angelinae jantan kontrol, P0 : Otus angelinae betina yang diberi suplemen serbuk kunyit 108 mg/ekor/ hari yang dicampur dalam pakan, P1 : Otus angelinae jantan yang diberi suplemen serbuk kunyit 108 mg/ekor/ hari yang dicampur dalam pakan Diamati perilaku dan kondisi fisiologis dengan menganalisis komponen kimia darah yaitu kadar hemoglobin, jumlah eritrosit, jumlah leukosit, pH darah, deferensial leukosit meliputi jumlah sel neutrofil, eosinofil, basofil, limfosit dan monosit, kadar kolesterol LDL,HDL, trigliserida, glukosa darah, SGPT,SGOT yang diambil pada akhir perlakuan. Dilakukan analisis deskriptif terhadap perilaku dan kondisi fisilogis Otus angelinae. Hasil penelitian menunjukkan pemberian suplemen serbuk kunyit tidak mempengaruhi perilaku celepuk jawa. Perbedaan perilaku terjadi pada siang dan malam hari selama dalam kandang. Perilaku siang hari: banyak istirahat, tidak nafsu makan, sangat waspada terhadap gangguan, sedangkan perilaku pada malam hari sangat agresif, makan lebih aktif. Pemberian serbuk kunyit dapat mempertahankan status fsiologis normal Otus angelinae jantan maupun betina.
Komunitas dan Preferensi Habitat Gastropoda pada Kedalaman Berbeda di Zona Litoral Danau Singkarak Provinsi Sumatera Barat Yuli Wendri; Jabang Nurdin; Indra Junaidi Zakaria
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p11

Abstract

This research was conducted in Lake Singkarak from February to March 2016, using purposive sampling method in three locations namely Tanjung Mutiara, Malalo and Paninggahan, at a depth of 0.5 m, 1 m and 1.5 m in each location. Samples were collected using 30x30 cm2 surber net. The result showed four gastropod species from family of Thiaridae, namely Melanoides granifera, Melanoides tuberculata, Brotia costula and Thiara scabra. Average population density of gastropod in Lake Singkarak was 399.75 ind / m2. The population density at depths ranging from 271.11 to 568.89 ind/m2, the highest population density is showed at a depth of 1.5 m and the lowest at a depth of 0.5 m. Melanoides tuberculata shows highest density at all depths. M. granifera prefers habitats with a depth of 1.5 m, M. tuberculata prefers a depth of 1 m, T. scabra prefers a depth up to 1.5 m and B. Costula prefer habitat with a depth of 0.5 m. Condition of Lake Singkarak shows as favorable and suitable habitat for gastropod’s life.
Karakteristik Dan Viabilitas Serbuk Sari 38 Ragam Tanaman Kamboja (Plumeria spp.) Di Bali I Komang Alit Adi Sanjaya; Eniek Kriswiyanti; Anak Agung Ketut Darmadi
Metamorfosa: Journal of Biological Sciences Vol 7 No 1 (2020)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2020.v07.i01.p06

Abstract

Penelitian ini bertujuan untuk mengetahui karakteristik dan viabilitas serbuk sari ragam tanaman kamboja (Plumeria spp.) di Bali. Pengamatan karakteristik serbuk sari dengan menggunakan metode asetolisis, sedangkan pengamatan viabilitas serbuk sari dengan metode warna 1 % anilin blue. Pengukuran dilakukan mengunakan mikrometri. Hasil pengamatan terhadap 38 ragam tanaman kamboja menunjukkan adanya variasi panjang aksis polar antara 36,48 ± 4,91 – 57,87 ± 3,89 µm, diameter bidang ekuatorial antara 30,18 ± 4,64 – 55,24 ± 5,52 µm, indeks P/E antara 0,85 ± 0,10 – 1,42 ± 0,13 serta viabilitas serbuk sari sebesar 6,67 – 42,31%. Presentase viabilitas serbuk sari tertinggi dijumpai pada ragam P. alba L. 'Snow White' sebesar 42,31 sedangkan terendah dijumpai pada ragam P. rubra L. 'Red Ajanta'. Berdasarkan indeks P/E serbuk sari tanaman kamboja dapat digolongkan kedalam kelompok subpheroidal hingga prolat. Berdasarkan panjang aksis polar serbuk sari tanaman kamboja dapat digolongkan kedalam kelompok media hingga magna.
Penurunan Kualitas Sperma Tikus (Rattus novergivus) yang Diinjeksi Vitamin C Dosis Tinggi dalam Jangka Waktu Lama Ni Wayan Sudatri; Dwi Ariani Yulihastuti; Ni Made Suartini
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p02

Abstract

Benefits of white vitamin C to increace the immune system is already exis. However, the use of high doses of vitamin C and in the long term can cause side effects.. The objective of this study was to determine the side effects of high-dose injections of vitamin C in the long term on sperm quality of rats. The design used in this study is completely randomized design (CRD) with long treatment injections of high doses of vitamin C are different. ie: P0 (control), P1 (injected 30 days), P2 (injected 50 days ), P3 (injected 70 days) and P4 (injected 90 days). Parameters measured were sperm quality (motility, viability, morphology, number) ,. The results showed that the sperm quality of rats (Rattus novergivus). Sperm quality of male rats (Rattus norvegivus L.) were injected vitamin C in high doses for long periods of time between the control and treatment groups showed significant differences (P = 0.000). The quality of sperm of mice injected high doses of white vitamin C tend to decrease sperm quality indicated by percentage of motility, viability, and morphology of spermatozoa although the concentration of spermatozoa tends to increase. Keywords: white vitamin C, rat, sperm quality
IDENTIFIKASI DAN PREVALENSI ENDOPARASIT PADA KAMBING DI KOTA PADANG, SUMATERA BARAT Marta Yufa; Mairawita Mairawita; Henny Herwina
Metamorfosa: Journal of Biological Sciences Vol 5 No 1 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i01.p14

Abstract

Kambing merupakan hewan ternak yang banyak dikonsumsi oleh masyrakat di Kota Padang. Pertumbuhan populasi kambing belum sebanding dengan angka permintaan yang terus meningkat, hal ini disebabkan umumnya kambing ini dipelihara secara tradisional dalam skala kepemilikan kecil sehingga pemeliharaannya kurang diperhatikan dan dapat menyebabkan kambing mudah terserang oleh parasit. Penelitian ini bertujuan untuk mengetahui jenis-jenis parasit pada kambing di Kota Padang serta mengetahui prevalensi masing-masing jenis. Penelitian ini dilakukan sejak bulan April hingga Mei 2016 di lima lokasi di Kota Padang dan Laboratorium Taksonomi Hewan Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Andalas. Sampel feses dikoleksi dari 25 ekor kambing, pemeriksaan sampel feses dilakukan dengan metode sentrifugasi-flotasi. Hasil penelitian didapatkan dua jenis endoparasit yaitu: Trichostrongylus sp. (nilai prevalensi 64%) dan jenis tidak teridentifikasi (Unidentified sp.) dari famili Taeniidae (nilai prevalensi 16%).
Histopatologi serta Aktivitas Hati Kelinci Lokal (Lepus sp.) yang Diberi Ransum Tepung Daun Kaliandra (Calliandra calothyrsus Meissn.) dan Kulit Nanas (Ananas comosus L.) I Gede Wiranatha; Iriani Setyawati; Ngurah Intan Wiratmini
Metamorfosa: Journal of Biological Sciences Vol 6 No 2 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i02.p07

Abstract

The aimed of this study was to determine the effect of feed containing various concentrations of Calliandra calothyrsus Meissn. leaf meal mixed with pineapple peels and concentrate feed on histopathology and the activity of liver of local rabbit (Lepus sp). This study used a Post-Test Only Control Group Design, using 24 local male rabbits divided into 4 groups of feed treatment namely P0 = concentrate feed as control; P1 = mixture of 15% of calliandra leaf meal (CLM), 30% of pineapple peels (PP) and 55% of commercial feed (CF); P2 = 30% of CLM, 30% of PP and 40% of CF; and P3 = 45% of CLM, 30% of PP and 25% of CF). The treatments were given for 60 days and used 6 replicates for each group. The data were analyzed statistically by SPSS program version 22. The research results showed the increase of calliandra leaf meal levels in concentrated feed which were added 30% of pineapple peels still caused liver damage namely fatty degeneration, necrosis and congestion. It increased the ALT level, but did not affect the AST level in local rabbit serum.
Effect of Heat Exposure on Percentage of Dead Sperm and Pyriform Cell in Male New Zealand White (NZW) Rabbit I Wayan Kasa
Metamorfosa: Journal of Biological Sciences Vol 5 No 2 (2018)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2018.v05.i02.p17

Abstract

Experiment has been done on the effect of heat exposure on the percentage of dead sperm and pyriform cell in male NZW rabbit. The bucks were exposed to 34°C for 8 hours on either 1 or 5 days. It was found that scrotal temperature (ScT) increased very rapidly during the first hour of exposure by 4°C in group 1 (1x8h). In group 2 (5x8h of exposure) ScT differed significantly between days (P < 0.05). The bigest response was on day 1 (36.6°C) and followed a progressive reduction, day by day, in an apparent acclimation pattern. At different times of exposure, ScT was observed to rise rapidly during the first hour (by 14 percent or 4.4°C). In the 1x8h treatment dead sperm achieved a maximum level of 14.5 ± 4.7 percent in the first week after hotroom treatment. In week 2, the percentage dead sperm started to decline. When rabbits were exposed to 5x8h, this group was more severely affected than the 1x8h. In week 1, the percentage dead sperm in the 5x8h group was 2.1 fold greater than in the 1x8h group (31.0 vs 14.5 percent). Recovery in the 5x8h group took longer than in the 1x8h group, and the percentage dead sperm finally returned to normal levels only after 7 weeks, compared to 5 weeks after 1x8h treatment.Between weeks, the incidence of pyriforms did differ significantly (P < 0.05). In the 1x8h treatment, the percentage pyriforms increased in the first week after hotroom exposure by 3.8 percent. In week 2, the percentage pyriform started to decline and continued to do so gradually until week 6. In the 5x8h treatment, the number of pyriforms was higher than it was in 1x8h treatment. In week 1, for example, the percentage pyriform was 16 percent higher than in 1x8h treatment group. Hence, the mean pyriform cell count was 1.6 times higher in the 5x8h compared to the 1x8h group.