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INDONESIA
METAMORFOSA Journal of Biological Sciences
Published by Universitas Udayana
ISSN : 23025697     EISSN : 26558122     DOI : -
Core Subject : Science, Agriculture, Social,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology
METAMORFOSA is an electronic scientific journal published periodically by the Master of Biology Udayana University, which includes scientific works in the field of Biology.
Arjuna Subject : -
Articles 316 Documents
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POTENSI Bacillus sp. B3 SEBAGAI AGEN BIOKONTROL PENYAKIT LAYU BAKTERI YANG DISEBABKAN OLEH Ralstonia sp. PADA TANAMAN CABAI (Capsicum annuum L.) Diah Kharismawati Djereng; Retno Kawuri; Yan Ramona
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p16

Abstract

Tanaman cabai (Capsicum annuum L.) merupakan salah satu komoditas hortikultura yang banyak dibudidayakan oleh petani di Indonesia. Kebutuhan cabai setiap tahun mengalami peningkatan, sedangkan produksinya sangat rendah, salah satu penyebab menurunnya produksi cabai adalah adanya gangguan penyakit layu bakteri, yang disebabkan oleh Ralstonia sp. Untuk menanggulanginya, maka perlu dikembangkan metoda biokontrol yang lebih aman dan ramah lingkungan. Penelitian ini bertujuan untuk mengisolasi dan mengidentifikasi patogen Ralstonia sp. pada tanaman cabai, serta untuk mengetahui efektivitas kultur Bacillus sp. B3 pada percobaan skala rumah kaca dalam menghambat bakteri Ralstonia sp. pada tanaman cabai. Djereng dkk. (2016), melaporkan Bacillus sp. B3 yang diisolasi dari produk CustomBio mampu menghambat Ralstonia sp. secara in vitro. Isolat Ralstonia sp. diisolasi dengan Platting Method menggunakan media selektif Sucrosa Peptone Agar (SPA) kemudian dilakukan uji Postulat Koch dan bakteri yang didapat dilakukan Uji pewarnaan Gram, uji Gula-gula, uji katalase, uji sitrat, dan hasil disesuaikan dengan buku identifikasi bakteri dari Holt et. al. (1994). Rancangan penelitian yang digunakan pada uji skala rumah kaca adalah Rancangan Acak Kelompok (RAK) dengan parameter yang diukur meliputi lebar daun, panjang daun, panjang akar, tinggi batang, dan diameter batang. Data pengukuran dianalisis dengan Analysis of Varian (ANOVA), kemudian dilanjutkan dengan uji Tukey taraf signifikan 5%. Hasil penelitian menunjukkan bahwa bakteri Ralstonia sp. berhasil diisolasi dan diidentifikasi dari tanaman cabai dengan gejala layu bakteri. Isolat Bacillus sp. B3 mampu menghambat penyakit layu bakteri pada tanaman cabai dengan penurunan insiden infeksi sebesar ± 100% (100% survive), relatif terhadap kontrol (pot yang diinokulasi dengan patogen saja) pada percobaan skala rumah kaca selama 4 minggu.
DESAIN TAQMAN PROBE SECARA IN SILICO SEBAGAI PENDETEKSI MUTASI PADA KODON 516 GEN rpoB Mycobacterium tuberculosis UNTUK METODE REAL-TIME PCR Dek Pueteri Dewi Suryani; Putu Sanna Yustiantara; Sagung Chandra Yowani
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p04

Abstract

The aim of this research was to in silico design of TaqMan probe. Design of TaqMan probe were conducted using software Clone Manager Suite 6. As a template, the rpoB gene of M. tuberculosis H37Rv (accession number U12205.1) was used. The results of this research were 8 sequences such as, R516MV-2, R516MV-3, R516MV-4, R516MV-5, R516MV-7, R516MV-8, R516MV-11, R516MV-13. These sequences were met the criteria of TaqMan probe, such as length of nucleotide (23-28 nucleotide), Tm value (72ºC), %GC (50-58%), runs and repeats (?4 bases), dimer structure in accordance to the requirements and does not form hairpin structures. In addition, these sequences were met labeling criteria of TaqMan probe which are including the location of G bases and the number of G-C bases in sequences. Therefore, these sequences could be labeled by FAM (reporter) at 5' end and TAMRA (quencher) at 3' end. The conclusion of this research, the sequences were met the criteria of TaqMan probe. Therefore, it could be targeted to detect mutations at codon 516 with a change of aspartic acid into valine (GAC ? GTC) by using real-time PCR method.
KEBERADAAN BAKTERI PATOGEN Vibrio cholerae PADA BEBERAPA HASIL PERIKANAN YANG DIJUAL DI PASAR TRADISIONAL KOTA DENPASAR I Wayan Yogi Widyastana; Retno Kawuri; Anak Agung Gde Raka Dalem
Metamorfosa: Journal of Biological Sciences Vol 2 No 1 (2015)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

The purpose of this research was to find out the existence of Vibrio cholerae, bacteria that may cause cholera disease, in some fishery products in Denpasar traditional market, Bali. This research used samples taken from three different fisheries products: tuna fishes (Euthynnus affinis), shrimps (Penaeus indicus), and shellfish (Anodonta sp.). They were taken from three traditional markets in Denpasar City: Ketapian, Kumbasari, and Pidada Markets. All samples were cultured on Alkaline Peptone Water (APW) media, continued by Thiosulfate Citrate Bile Salt Sucrose (TCBS), and then Biochemical Test and Serology Test undertaken. The results of this study showed that two (7.4%) samples taken from Ketapian Market were proved to be positive containing pathogenic bacteria of V. cholerae; they were the shrimps with UA2 code and the shellfish with KA2 code. Meanwhile, there were no V. cholerae contaminations proven to exist in two other kind of products in other two traditional markets.
HISTOLOGI TESTIS TIKUS (Rattus norvegicus) JANTAN YANG DIBERI TEPUNG DAUN LAMTORO (Leucaena leucocephala Lamk. de Wit) HASIL PERENDAMAN A.A. Istri Mas Padmiswari; Ngurah Intan Wiratmini; I Wayan Kasa
Metamorfosa: Journal of Biological Sciences Vol 4 No 2 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i02.p07

Abstract

Lamtoro merupakan salah satu tanaman yang memiliki kandungan protein yang cukup tinggi yaitu berkisar antara 25-35%. Namun, pemanfaatan lamtoro menjadi terbatas karena mengandung zat antinutrisi seperti mimosin. Kandungan mimosin dapat diturunkan melalui beberapa metode salah satunya adalah melalui perendaman dalam air. Penelitian ini bertujuan untuk mengetahui pengaruh dari pemberian tepung daun lamoro hasil perendaman (TDLP) terhadap histologi testis tikus jantan. Rancangan percobaan yang digunakan adalah Rancangan Acak Lengkap (RAL) yang terdiri dari empat kelompok dengan masing-masing delapan ulangan. Perlakuan berupa pemberian tepung daun lamtoro hasil perendaman yang dicampur dengan pelet komersial dengan aras 100% pakan komersial (tanpa TDLP) sebagai kontrol (P0), 92,5% pakan komersial + 7,5% TDLP sebagai perlakuan 1 (P1), 85% pakan komersial + 15% TDLP sebagai perlakuan 2 (P2) dan 77,5% pakan komersial + 22,5% TDLP sebagai perlakuan 3 (P3). Perlakuan diberikan pada tikus jantan selama 30 hari. Variabel yang diamati pada penelitian ini adalah jumlah sel spermatogenik. Data hasil penelitian diolah menggunakan program statistik komputer (SPSS 16.0 for Windows) dengan menggunakan uji One Way Anova. Hasil penelitian menunjukkan bahwa penambahan TDLP dalam ransum tidak berpengaruh nyata (P>0,05) terhadap jumlah sel spermatogenik. Hal ini menunjukkan bahwa penambahan TDLP hingga aras 22,5% tidak menurunkan jumlah sel spermatogenik.
PENGARUH KONSENTRASI EKSTRAK KASAR KULIT BATANG KUSAMBI (Schleichera oleosa (Lour) Oken) TERHADAP PERTUMBUHAN IN VITRO BAKTERI E. coli Ni Made Susilawati; Yan Ramona; I Made Oka Adi Parwata
Metamorfosa: Journal of Biological Sciences Vol 3 No 2 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i02.p06

Abstract

The main objective of research was to investigate the effectiveness of Schleichera oleosa (Lour) Oken crude extract to inhibit the in vitro growth of E. coli. The crude extract of the plant and the E. coli used in this study were obtained by applying the method of maceration in methanol solution and the method of dilution and spread plate on nutrient agar (NA) medium, respectively. The in vitro bioassay of the extract on E. coli was conducted by applying the method of diffusion on NA medium. The results showed that the crude extract of Schleichera oleosa (Lour) Oken was found to be effective to inhibit the growth of E. coli in the in vitro tests. The diameter of inhibition zone was proportional with the extract concentration exposed to the E. coli.
PERUBAHAN ANATOMI STOMATA DAUN LILI TRUMPET (Lilium longiflorum) SETELAH PEMAPARAN RADIASI SINAR X Ni Kadek Dwipayani Lestari; Ida Ayu Astarini; I.G.M Oka Nurjaya
Metamorfosa: Journal of Biological Sciences Vol 1 No 1 (2012)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Lily (L. longiflorum) is one of 280 genus of liliaceae family. Variation on flower colour, growth habit, flowering period (shelf life) is very limited in Indonesia, so that plant breeding techniques are required to obtain a wide variety of plants. Physical mutation, radiation with X rays were employed in this study. The aim of this study is to find out the effect of X-ray exposure on lily’s leaves stomata anatomy. Lily seeds were grown in vitro and seedlings were exposed to X ray. This study employ CRD (completely randomized design) with four dose exposure (0 krad, 5 krad, 7,5 krad dan 10 krad) and 10 replicates each dose. Results show that to X ray causing reduction in stomata’s length, stomata amount, index, density of stomata that occurs randomly, compared to control.
POTENSI Salmonella typhi YANG DILEMAHKAN DENGAN SINAR ULTRAVIOLET SEBAGAI VAKSIN ALTERNATIF Andreas Putro Ragil Santoso; Retno Kawuri; I Nengah Kerta Besung
Metamorfosa: Journal of Biological Sciences Vol 3 No 1 (2016)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2016.v03.i01.p05

Abstract

Salmonella typhi is a Gram-negative intracellular bacterium and causes typhoid fever in humans. The success rate of Berma Vivotif Ty21a vaccine in Indonesia is only 33-66%, while in other countries have been reached up to 100%. The research was conducted in order to determine the potency of local isolate bacteria to stimulate the immune response and the impact of different exposure frequencies on the immune response and the different immune response time when administered by UV-inactivated vaccine.. The results showed that the antibody titer of local isolates irradiated by UV light 10x was 88.76 ± 33.06 IU/mL at week 4 with the lowest antibody titer values about 11.15 ± 9.18 IU/mL was found in the negative control.
ANALISIS KANDUNGAN FENOLIK EKSTRAK DAUN JATI (Tectona grandis L.) DENGAN WAKTU DEKOMPOSISI YANG BERBEDA Ni Putu Adriani Astiti
Metamorfosa: Journal of Biological Sciences Vol 4 No 1 (2017)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2017.v04.i01.p18

Abstract

Teak (Tectona grandis L.) is a plant with strong wood and is resistant to pests and diseases. In addition, its leaf extract is widely used by public as an additive in foods both as a preservative and as an alternative food coloring. The objective of this research was to determine phenolic compounds of teak leaves extracted with three different decomposition times (one, two and three weeks of decomposition). Extraction was conducted by using maceration method in which water was used as solvent. Identification of Phenolics was done by using Gas Chromatography. The results showed that the compounds of vanilic acid, salicylic acid, ferulic acid, cumaric acid, gallic acid, benzoic acid and cafeic acid were identified in the analysis. The concentration and composition of phenolic compounds in the extract was determined by decomposition.
Mekanisme Kerja Antibiotik Ceftriaxone, Clarithromycin, dan Senyawa Aktif Quercetin pada Streptococcus pneumoniae dengan Metoda Docking In Silico Irma Damayanti; Yan Ramona; Sentot Joko Raharjo
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p01

Abstract

Pneumolysin is a protein that plays an important role in cell death and bacterial colonization of Streptococcus pneumoniae. Ceftriaxone, clarithromycin, dan quercetin has been proven (in vivo and in vitro) to prevent the action of pneumolysin. The purpose of this study was to identify interactions between ceftriaxone, clarithromycin, and quercetin in inhibiting the pneumolysin activity through in silico. The 3D structure of pneumolysin was downloaded via RCSB PDB (PDB ID:PQQA), while 3D structure of ceftriaxone (ID:5479530), clarithromycin (ID:84029), and quercetin (ID:5280343) were downloaded via PubChem. Protein preparations were done with Discovery Studio, while ligands were prepared with OpenBabel. The docking process used HEX 8.0.0 and visualization analysis with Discovery Studio. Pneumolysin-ceftriaxone interaction produces 4 hydrogen bonds, 5 hydrophobic bonds, 1 electrostatic bond, and 2 unfavorable residue bonds with a bond energy value -329.98kJ/mol. Pneumolysin-clarithromycin interaction produces 3 hydrogen bonds, 1 electrostatic bond, and 3 unfavorable residue bonds with a bond energy value -311.12kJ/mol. Pneumolysin-quercetin interaction produces 2 hydrogen bonds, 4 hydrophobic bonds, and 2 electrostatic bonds with a bond energy value -246.52kJ/mol. Keyword: pneumolysin, ceftriaxone, clarithromycin, quercetin, in silico.
Analisis Kandungan Klorofil-A dan Kualitas Air Waduk Ciwaka Kota Serang Banten Sakhirotul Lail; Aditya Rahman KN; Rida Oktorida Khastini
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p15

Abstract

The research was conducted to determine the quality of the waters of the Ciwaka Reservoir, Serang City, Banten, based on the content of chlorophyll-a and the presence of phytoplankton. The relationship between the chlorophyll-a content and the presence of phytoplankton with the physical-chemical parameters of the waters was also being analyzed. Thus the results from the study were used as content for learning media education research. This research was conducted from February-July 2021, in Ciwaka Reservoir, Serang City, Banten. A sampling of water at three stations was carried out using the purposive sampling method. Chlorophyll-a analysis was carried out using spectrophotometric methods. The results of chlorophyll-a analyze in each station ranged from 7,458 g/L – 34,352 g/L. Ciwaka Reservoir is included in the category of waters that are eutrophic (polluted) due to high nutrient levels. The water quality of Ciwaka Reservoir is still in good condition according to the designation of class 2 water quality standards in PP No. 82 of 2001. The content of chlorophyll-a and the presence of phytoplankton (abundance, diversity, and dominance) only has a weakness with the brightness (r = 0.000). Phytoplankton diversity was strongly correlated with temperature (r = 0.994). Phytoplankton dominance was strongly related to DO (r = 0.998) and BOD (r = 0.998). Keyword: Eutrophic, Chlorophyll-a, Ciwaka Reservoir

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