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Pertumbuhan Kantong Semar (Nepenthes mirabilis (Lour.) Druce) Pada Berbagai Media Tanam Rina Budi Astuti; Sri Widodo Agung Suedy; Yulita Nurchayati; Nintya Setiari
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p06

Nepenthes mirabilis (Lour.) is a unique plant with a pitcher at the tip of the leaf. This species belongs to a rare category so needed to be preserved. One of the conservation efforts are the selection of the composition growing media for cultivation. This study aimed to found alternative planting media that can support the growth of Nepenthes other than sphagnum moss media. The research was conducted at the green house in Ngablak Village, Ngablak District, Magelang Regency from August to November 2020. This study used a Completely Randomised Design (CRD) with 4 treatments, sphagnum moss, roasted husk, roasted husk with cocopeat (3:1), sphagnum moss with cocopeat (3:1) given of 4 replications. Research parameter include, number of leaves, leaf length, leaf width, and plant height measured at 12 Weeks After Planting (WAP). Observation data were analysis by Analysist of Varians (ANOVA) and Duncan Multiple Range Test (DMRT) on a confidence level of 95%. The result showed that all treatment had a significant effect on the number of leaves, leaf length, leaf width, and plant height. The best planting medium was sphagnum moss with the time of appearance of the leaves (2,39 week), the highest number of leaves (11,25 sheet), and plant height (1,6 cm). the mixture media of roasted husk and cocopeat (3:1) produced growth that wsn’t different from sphagnum moss, so it was recommended to be a cheaper alternative media. Keyword: Nepenthes mirabilis, sphagnum moss, roasted husks, cocopeat

Pertumbuhan dan Rendemen Minyak Atsiri Tanaman Selasih (Ocimum basilicum L.) pada Naungan yang Berbeda Ryan Qony Dharmawan; Nintya Setiari; Sri Haryanti
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p11

Selasih merupakan tanaman penghasil minyak atsiri. Sintesis minyak atsiri dipengaruhi oleh faktor lingkungan seperti cahaya dan suhu. Pemberian naungan dapat mempengaruhi intensitas cahaya dan suhu yang diterima tanaman. Tujuan dari penelitian ini adalah untuk mengkaji pemberian naungan yang berbeda dalam mempengaruhi pertumbuhan dan rendemen minyak. Penelitian ini merupakan penelitian experimental menggunakan rancangan acak lengkap (RAL) dengan pola tunggal yaitu naungan dengan parameter pertumbuhan dan pola faktorial dengan faktor pertama yaitu naungan dan faktor kedua yaitu organ tanaman dengan parameter rendemen minyak atsiri. Penanaman dilakukan dengan penyemaian benih selama 2 minggu kemudian dipindahkan ke polybag yang berukuran 30 cm x 30 cm yang berisi media tanam berupa campuran tanah, cocopeat, sekam bakar, dan kompos dengan perbandingan 1:1:1:1 dan diberi pupuk kandang sebanyak 30 gr. Perlakuan naungan menggunakan naungan 0%, 25%, dan 50% untuk mengetahui pengaruh pertumbuhannya, sedangkan rendemen minyak diambil bagian daun, batang, dan bunga untuk mengetahui interaksi antara naungan dan rendemen minyak atsiri pada organnya. Data dianalisis menggunakan Analysis of Variance (ANOVA) dengan taraf kepercayaan 95%, dan jika ada beda nyata dilanjutkan dengan uji Duncan’s Multiple Range Test (DMRT). Parameter data yang diambil adalah jumlah daun, waktu inisiasi bunga, tinggi tanaman, jumlah cabang primer, luas daun, berat kering, berat basah, dan rendemen minyak atsiri (daun, batang, dan bunga). Hasil penelitian menunjukan terdapat pengaruh nyata pada parameter tinggi tanaman, luas daun, waktu inisiasi bunga, dan rendemen minyak atsiri. Rendemen minyak tertinggi pada bagian bunga tanaman dengan naungan 50%.

Potensi Genitri (Elaeocarpus sphaericus Schum.) pada Leukosit dan Struktur Jaringan Paru-Paru Tikus Wistar Induksi Virus Influenza Cicilia Novi Primiani; Pujiati Pujiati; Mohammad Arfi Setiawan
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p02

Genitri (Elaeocarpus sphaericus Schum.) is an endemic plant of Indonesia that has not been widely cultivated and utilized. The use of genitri is usually as road shade, firewood, seeds for jewelry. The results showed that the diversity of chemical compounds genitri had potential for health. The aim of the study was to analyze the potential of genitri to leukocytes and lung tissue structure of wistar rats induced by influenza virus. The study used an experimental approach, with experimental animals in the form of 24 male wistar rats in 4 treatment groups, P0 negative control, P1 influenza virus induction (positive control), P2 influenza virus induction and given genitri leaf, and P3 influenza virus induction and given genitry seeds. Virus induction on day 0 and day 14. Leaves and seeds were given on the 3rd day. Surgery was performed on day 5 and day 33. The number of leukocytes were calculated using microhematocrit, and identified leukocytes and lung tissue structure staining with HE staining. The results showed that genitry seeds could increase the number of leukocytes up to 13,000 cells/µL, the size of the alveoli diameter was 31.48 m, the size of the alveolar septum was 12.02 m. Keywords: Antiviral, Elaeocarpus Sphaericus Schum, Genitri, Leukocytes, Pulmo

Comparison of Three DNA Isolation Methods of Aspergillus Niger Adyan Donastin; Maharani Pertiwi Koentjoro; Muhamad Taufik Hidayat; Endry Nugroho Prasetyo
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p07

Polymerase Chain Reaction (PCR) is a technique that is applied to detect and test the presence of genetic material from pathogenic fungi. This method was further developed because it has a high sensitivity compared to the culture method in detecting the presence of pathogenic fungi. To perform sensitive, specific and reliable PCR-based assays, the availability of pure DNA as well as an easy-to-perform DNA extraction protocol is essential. Currently, existing protocols for DNA extraction generally require specialized kits and with the addition of enzymes. Therefore, this study was conducted to compare the quantity and quality of Aspergillus niger DNA isolation with three different methods. The stages of the research carried out included pure A. niger culture and total DNA isolation using three methods, namely the protocol according to the Wizard® Genomic DNA Purification Kit (P1) instructions, Modified Wizard® Genomic DNA Purification Kit (P2) and Monarch Genomic DNA Purification Kit NEB (P3). The results of the evaluation of DNA isolation using a spectrophotometer at a wavelength of A260/280 nm showed a ratio of 1.4, 1.8, and 1.7 at P1 and P2, and P3 respectively. The quantity obtained ranged from 210 to 305 ng/µL. The total DNA results obtained were then used for PCR testing of ITS ribosomal DNA fragments using ITS1 and ITS4 primers. Electrophorosis observation of PCR results showed that all samples produced bands with a length of 500 bp. The results of DNA isolation in the P1 method did not show bands on the agarose gel, but could be seen in the PCR product. Methods P2 and P3 showed DNA that had good quality and quality. The P2 and P3 methods use a cell destruction technique with liquid nitrogen and a combination of addition of proteinase K. The obtained protocol is expected to provide fast and good method information in the isolation of total DNA from A. niger. Keyword: Aspergillus niger, total DNA isolation, ITS1, ITS4

INVESTIGASI BAHAN AKTIF PEGAGAN BERDASARKAN JEJARING DENGAN PROTEIN TARGET : STUDI PENCARIAN OBAT ALZHEIMER SECARA IN SILICO Moch Mustakim; Vina Fitria Wulandari; Nabilah Miftachul Khoiriyah; Azis Mawardi; Rossa Martha Wulandari; Thiya Fathiyatul Fauziyah; Nurul Jadid Mubarakati
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p12

INTISARI Penyakit Alzheimer merupakan penyakit neurodegeneratif yang mematikan peringkat 10 teratas berdasarkan WHO tahun 2019. Alzheimer ditandai dengan adanya penurunan aktifitas Choline Acetytransferase (ChAt) yakni zat yang berperan dalam pembentukan enzim asetikolin sebagai enzim. Salah satu bahan alam yang diketahui berperan dalam meningkatan daya ingat yaitu Pegagan. Untuk mengetahui jalur mekanisme senyawa aktif pegagan dalam pengobatan Alzheimer, penelitian ini menggunakan pendekatan in silico dengan menganalisis protein target dan senyawa aktif pegagan dalam suatu jejaring interaksi protein-senyawa. Penelitian ini menggunakan 12 senyawa uji dan molekul reseptor ChAt yang digunakan yaitu 5 TPG dan 4 KMD. Desain jejaring interaksi protein-senyawa dilakukan menggunakan sea target dan STRING database. Hasil interaksi lalu divisualisasikan di perangkat cytoscape. Hasil penelitian ini ditemukan terdapat 8 senyawa uji yang memiliki interaksi dengan reseptor ChAt sedangkan protein target yang berhubungan langsung dengan reseptor ChAt yaitu Tryptophan 5-hydroxylase 1 dan Zinc finger protein GLI. Jadi, senyawa aktif pegagan yang paling banyak berperan berdasarkan protein targetnya adalah senyawa Asiatic Acid, Brahmic Acid, -Humulene, -Caryophyllene, Bicyclogermacrene, Germacrene B, - Pinene, Caryophyllene. Kata kunci: penyakit Alzheimer, Choline Acetytransferase (ChAt), Pegagan, in silico, Jejaring interaksi

Komposisi Floristik Vegetasi di Kawasan Hilir Sungai Unda, Kabupaten Klungkung, Bali I Made Sara Wijana; I Made Saka Wijaya; Abd. Rahman As-syakur; Gede Surya Indrawan; Putu Angga Wiradana; Komang Kartika Indi Swari; Dewa Ayu Intan Tirta Sari
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p03

The downstream area of Unda River Watershed located in Klungkung Regency has experienced succession due to the eruption of Mount Agung in 1963. The ecosystem in the downstream area of the Unda River also experienced anthropogenic succession due to human activities which affected the plant species that composing the vegetation. This study aims to observe the floristic composition of vegetation in the downstream area of the Unda River. The research was conducted by exploration method in three types of ecosystems: lowland forest, marshes, and beaches. The results show the floristic composition in the downstream of Unda River composed by 163 plant species, classified in 48 families and 128 genera. The major growth form is small herb (47 species), grass (35 species), and tree (31 species). Fabaceae is the plant family with the most abundant species, followed by Poaceae and Cyperaceae. Based on the research zones, the lowland forest has the highest number of plant species (120 species), while the beach zone has the lowest (82 species). The ecosystem stability in the downstream area of Unda River can be disturbed by the existence of invasive species, such as Eichhornia crassipes, Chromolaena odorata, Lantana camara, Ipomoea carnea, Vachellia farnesiana, and Typha angustifolia.

Uji Efektivitas Vitamin C sebagai Neuroprotektor pada Mencit (Mus musculus) yang Diinduksi Akrilamida Salsabillah Rahma; Ngurah Intan Wiratmini; Ni Wayan Sudatri
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p08

Acrylamide is a contaminant compound found in several types of food containing carbohydrates and processed food through frying or roasting at high temperatures. Acrylamide compounds could cause the formation of oxidative stress which could lead to neurotoxicity. This oxidative stress could be minimized by administering the antioxidant vitamin C. This study was conducted to determine the mice cerebral cortex histology, the mice cerebellum histology induced by acrylamide and vitamin C supplementation, and the most effective dose as a neuroprotector. This study used a completely randomized design (CRD) with 4 groups, namely K + (acrylamide 12.5 mg / kg BW); K- (NaCl 0.9%); P1 (vitamin C dose of 0.26 mg/animal /day + acrylamide dose of 12.5 mg/kg BW); and P2 ((vitamin C dose of 0.52 mg/animal/day + acrylamide dose of 12.5 mg / kg BW) and 6 repetitions so that the number of mice used was 24. The acrylamide and vitamin C solutions were administered orally, and acrylamide given for 20 days, while vitamin C was given 3 days before acrylamide induction until day 20. Mice were dissected at day 21, brain organs were collected for histological preparations by the paraffin method and Hematoxylin-Eosin staining. The qualitative data obtained were analyzed by One Way ANOVA and continued with the Duncan test. The parameters observed were necrosis in pyramid cells and glial cells. The results showed that vitamin C at dose of 0.52 mg/animal/day significantly repaired the damage in pyramid cells and glial cells induced by acrylamide.

IPerforma Larva Lalat Tentara Hitam (Hermetia illucens) sebagai Biokonversi Limbah Industri Pengolahan Carica Dieng (Vasconcellea pubescens) di Wonosobo Irma Fatmanintyas; Trisnowati Budi Ambarningrum; Atang Atang; Trisno Haryanto; Eko Setiyono
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p13

Limbah berupa kulit dan biji yang berasal dari industri pengolahan carica Dieng (Vasconcellea pubescens) dapat menjadi permasalahan lingkungan apabila tidak tertangani dengan baik . Larva BSF (Hermetia illucens) sebagai agen biokonversi diharapkan mampu menjadi solusi untuk permasalahan tersebut. Penelitian dilakukan secara eksperimental dengan menggunakan rancangan acak lengkap (RAL) dengan 6 perlakuan dan 4 ulangan meliputi perlakuan kontrol, P1 = 100% kulit, P2 = 75% kulit + 25% biji, P3 = 50% kulit + 50% biji, P4 = 25% kulit + 75% biji, dan P5 = 100% biji. Waktu pengamatan penelitian dilakukan selama 21 hari. Parameter utama yang diamati adalah nilai konsumsi pakan dan indeks pengurangan limbah (WRI), sedangkan parameter pendukung berupa biomassa larva, diameter kapsul kepala dan tingkat kelulusan hidup (survival rate). Data yang diperoleh dianalisis menggunakan ANOVA dengan tingkat kesalahan 5%, dan apabila perlakuan berpengaruh nyata, dilanjutkan dengan uji DMRT dengan tingkat kesalahan 5%. Hasil penelitian dengan jenis pakan yang bervariasi menunjukkan nilai konsumsi pakan tercerna oleh larva BSF berkisar antara 60,42% - 81,26%. Sedangkan untuk nilai indeks pengurangan limbah (WRI) berkisar antara 2,82% - 3,73%. Nilai reduksi limbah lebih dari 50% menunjukkan adanya efektivitas larva BSF dalam mendegradasi limbah organik. Dengan demikian penggunaan jenis pakan berupa pakan penggunaan larva BSF efektif dalam mereduksi limbah pengolahan carica Dieng (V. pubescens).

Pengaruh Lama Penyimpanan Dengan Pemberian Perasan Jeruk Nipis Terhadap Jumlah Angka Bakteri Pada Daging Ayam Kampung Nadya Treesna Wulansari; AA Istri Mas Padmiswari; Ni Wayan Sukma Antari
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p04

The study aims to identify effect of length of storage by giving lime juice on the number of bacterial in domestic chicken meat. Experiments with a completely randomized design model were used in this study. The results is indicated that there was an effect of length of storage by giving lime juice on the number of bacteria in domestic chicken meat. The content of organic acids, citric acid, essential oils and limonene contained in lime juice affects the total bacteria found in domestic chicken meat. Domestic chicken meat with lime juice which was stored at <40C for 12 days tended to have an increasing in the number of bacteria contaminants. However, the total number of bacterial plates in domestic chicken meat did not exceed the quality standard limit of the microbial contamination level issued by the Indonesian National Standardization Agency, which is 1 x 106 CFU / g. The number of bacteria in domestic chicken meat based on the length of the storage will tend to increase Keyword: length of storage, lime juice, bacteria, domestic chicken meat

POTENSI Gracilaria canaliculata SEBAGAI PENGHASIL BIOETANOL ASAL PANTAI MENGANTI KEBUMEN DAN PANTAI KARANG BOLONG CILACAP Rizal Berlian Novella; Dwi Sunu Widyartini; Romanus Edy Prabowo
Metamorfosa: Journal of Biological Sciences Vol 9 No 1 (2022)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2022.v09.i01.p09

Seaweed is a source of foreign exchange and a source of income for coastal communities. Besides being used as food, drink, and medicine, seaweed which is rich in cellulose is very useful to become bioethanol. Bioethanol is used as a raw material for the manufacture of ethanol derivatives. The research objective was to sea the biomass and bioethanol content in Gracilaria canaliculata seaweed from Karang Bolong Beach, Cilacap, and Menganti Beach, Kebumen. Analysis of bioethanol data was carried out using the T-test with the SPSS program to determine whether there were differences in the bioethanol content of Gracilaria canaliculata from Menganti Beach, and Karang Bolong Beach, while the environmental monitoring test with biomass used the PRIMER 7 program for the most influential environmental factors. The results of the study showed there is evidence of environmental factors at Menganti Kebumen Beach and Gracilaria canaliculata biomass, the most influential of which are nitrate. The results of the test to maintain environmental factors of Menganti with Gracilaria canaliculata seaweed biomass using the BIOENV Primer 7 program analysis showed a fixed value on the nitrate display with a value of 0.852 while in the waters of Karang Bolong the value was recorded at 0.79. The T-test results showed no significant difference in the bioethanol content of Gracilaria canaliculata from Menganti Beach, Kebumen, and Karang Bolong Beach, Cilacap. Gracilaria canaliculata from Menganti Beach, Kebumen, produced an average bioethanol content of 7.07%, while those from Karang Bolong Beach Cilacap produced an average bioethanol content of 7.21%.

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Journal Mail Official
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Kota denpasar,

Bali

INDONESIA