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Contact Name
ARI HAYATI
Contact Email
ari.hayati@unisma.ac.id
Phone
+62341- 551932
Journal Mail Official
biosaintropis@unisma.ac.id
Editorial Address
Tata Usaha FMIPA Unisma Gedung Usman bin Affan Kompleks Unisma Jl. MT Haryono 193 Malang 65144
Location
Kota malang,
Jawa timur
INDONESIA
Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)
ISSN : 23382805     EISSN : 24609455     DOI : https://doi.org/10.33474/e-jbst.v7i2.305
Core Subject : Agriculture, Social,
Jurnal ini mengkaji fenomena dan temuan penelitian di bidang biologi dan ilmu-ilmu dasar (sains) lainnya serta bidang studi di wilayah tropis. Jurnal ini ditujukan untuk menemukan solusi alternatif dalam perkembangan ilmu biologi demi kesejahteraan masyarakat Indonesia dan Dunia.
Articles 270 Documents
Kondisi Lingkungan dan Persepsi Masyarakat Perairan Tambak Garam di Dusun Ageng Desa Pinggir Papas, Sumenep Madura Rohmi Romdana; Ahmad Syauqi; Husain Latuconsina
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 1 (2020): Kondisi Habitat
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Salt is one of the strategic commodity products for human life today, not only used as consumption, salt is now widely used as material for industry, beauty products, agriculture and health. Determination of salt quality depends on the condition of the waters and environmental conditions that are made as a place of salt production. The purpose of this research is to determine the initial conditions of salt pond waters and community perceptions about the environmental conditions in Ageng hamlet of Pinggir Papas village, Sumenep Madura. The method used explorative quantitative descriptive and data collection techniques in this study using purposive sampling. Data retrieval is done at 3 stations (pond) with duplo tests. The results of the measurement of the average initial conditions of salt pond waters in the village of Ageng obtained temperatures between 31-34 ° C, turbidity of 5.80-7.91 NTU, conductivity 1985-1988 µS / cm, pH 7.2-7.5, COD 20.45-26.23 mg / L, 2-4 plastic particles, coliform 15-150 / 100 ml and for the value of perception found high values. The condition of the waters and the environment of salt ponds in the Ageng hamlet shows conditions that are classified as normal limits to mild contamination for raw materials for salt production. Keywords: salt production, water conditions and community perception ABSTRAK Garam meupakan salah satu produk komodatis yang strategis bagi kehidupan manusia saat ini, tidak hanya digunakan sebagi konsumsi, garam saat ini sudah banyak di gunakan sebagai bahan untuk industri, produk kecantikan, pertanian dan kesehatan. Penentu kualitas garam tergantung dari kondisi perairan dan kondisi lingkungan sekitar yang di jadikan sebagai tempat produksi garam. Tujuan penelitain ini yaitu untuk mengetahui kondisi awal perairan tambak garam dan persepsi masyarakat tentang kondisi lingkungan di dusun Ageng desa Pinggir Papas, Sumenep Madura. Metode yang diguankan pada penelitain yaitu metode deskriptif kuantitatif ekploratif dan teknik pengumpulan data pada penelitian ini menggunakan purposive sampling. Pengambilan data dilakukan pada 3 stasiun (Kolam) dengan ulangan Duplo. Hasil rata-rata pengukuran kondisi awal perairan tambak garam di dusun Ageng diperoleh suhu antara 31-34 °C, kekeruhan 5,80-7,91 NTU, Konduktivitas 1985-1988 µS/cm, pH 7,2-7,5, COD 20,45-26,23 mg/L, plastic 2-4 partikel, coliform 15-150/100 ml dan untuk nilai persepsi didapati nilai yang tinggi. Kondisi perairan dan lingkungan tambak garam di dusun Ageng menunjukan kondisi yang tergolong batas normal hingga tercemar ringan untuk bahan baku produksi garam. Kata Kunci: produksi garam, kondisi perairan dan persepsi masyarakat
Analisis Viabilitas Spermatozoa Sapi Friesian Holstein (Bos taurus) Post Thawing Semen Beku Dengan Pengaruh Suhu dan Lama Waktu Thawing Berbeda Dewi Malinda; Hari Santoso; Husain Latuconsina
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 2 (2021): Memperjelas Fenomena
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

The principle of freezing semen is to maintain the survival of sperm in the long term (long term preservation). Thawing is the process of thawing frozen cattle semen before it is used in the artificial insemination process (IB). This thawing has a big influence on the state of sperm, especially on the integrity of sperm in semen, depending on the temperature and length of time. The choice of temperature and length of time thawing is good and right can prevent damage to spermatozoa. The purpose of this study was to analyze the effect of differences in temperature and the length of post thawing time on the viability of FH (Frisian Holstein) cow spermatozoa and to find the optimal temperature and length of thawing time in the IB process. The research method was experimental using RAK with 3 treatment groups and 2x replications. The research material used frozen semen of FH cattle from the Singosari Center for Artificial Insemination (BBIB). The observed variable was Viability with Eosin staining of 2%. Data analysis used the Factorial ANOVA test. The results of this study are the length of time thawing did not differ significantly namely P> 0.05, temperature 25 ° C 20 seconds: 66.30%, 25 ° C 30 seconds: 66.80%, 25 ° C 40 seconds: 63, 30%, 37 ° C 20 seconds: 65.00%, 37 ° C 30 seconds: 74.30%, 37 ° C 40 seconds: 69.00%, 40 ° C 20 seconds: 75.50%, 40 ° C 30 seconds: 65.50%, 40 ° C 40 seconds: 67.50%, the highest percentage of life (viability) of spermatozoa is at 40°C with a duration of 20 seconds with a value of 75.50%. Conclusions the higher temperature with the short thawing time can support the high viability. Keywords: Bos taurus, Frozen semen, Thawing, Spermatozoa Viability ABSTRAK Prinsip pembekuan semen adalah untuk mempertahankan kelangsungan hidup sperma dalam jangka waktu yang lama (long term preservation). Thawing adalah proses pencairan kembali semen beku sapi sebelum digunakan dalam proses inseminasi buatan (IB). Pencairan ini memiliki pengaruh besar pada keadaan sperma, terutama pada integritas sperma dalam semen, tergantung pada suhu dan lamanya waktu. Pemilihan suhu dan lama waktu thawing yang baik dan tepat dapat mencegah kerusakan spermatozoa. Tujuan penelitian untuk menganalisa pengaruh perbedaan suhu dan lama waktu post thawing terhadap viabilitas spermatozoa sapi FH (Frisian Holstein) dan mencari suhu dan lama waktu thawing yang optimal dalam proses IB. Metode penelitian dilakukan secara eksperimental menggunakan RAK dengan 3 perlakuan kelompok dan 2x ulangan. Bahan penelitian menggunakan semen beku sapi FH dari Balai Besar Inseminasi Buatan (BBIB) Singosari. Variabel yang diamati adalah Viabilitas dengan pewarnaan Eosin 2%. Analisis data mengguakan uji ANOVA Faktorial. Hasil penelitian diperoleh lama waktu thawing tidak berbeda secara signifikan yaitu P>0,05, suhu 25°C 20 detik : 66,30%, 25°C 30 detik : 66,80%, 25°C 40 detik : 63,30%, 37°C 20 detik :65,00%, 37°C 30 detik :74,30%, 37°C 40 detik : 69,00%, 40°C 20 detik : 75,50%, 40°C 30 detik : 65,50%, 40°C 40 detik : 67,50%, presentase hidup (Viabilitas) spermatozoa tertinggi terdapat pada suhu 40°C dengan lama waktu 20 detik dengan nilai 75,50%. Semakin tinggi suhu dengan waktu thawing yang singkat dapat mendukung tingginya viabilitas. Kata Kunci: Bos taurus, Semen beku, Thawing, Viabilitas Spermatozoa
Analisis Normalitas dan Abnormalitas Spermatozoa Sapi Friesian Holstein (Bos taurus) Tahap Post Thawing Motility pada Suhu dan Lama Waktu Berbeda Menggunakan Pengencer Andromed Sabilah Safitri; Hari Santoso; Husain Latuconsina
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 2 (2021): Memperjelas Fenomena
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

The success of Artificial Insemination (ai) depends on the quality of cement that will be used for the technique of Artificial Insemination, and semen quality is seen from the normality and abnormality of spermatozoa.. From the results obtained in this study, namely Abnormality of spermatozoa of cattle FH for a long time thawing are not significantly different (P=0.05), with the value of the average percentage of abnormality high of 4.25 ± 0,354 obtained in the long time of 40 seconds, and the average value of the percentage of the best achieved of 1.75 ± 0,354 with the time of 20 seconds. Long-time thawing against the normality of spermatozoa of cattle FH not significantly different (P=0.05), it can be seen from the value of the average percentage of normality highest achieved 98.3 ± 0.354 with the time of 20 seconds. The percentage of normality of the lowest obtained value of the average percentage to 96.0 ± 0.707 with the time of 20 seconds. But the temperature obtained in the both Abnormality and Normality is significant (P=0.05). It can be seen the results of Abnormality high in get at Temperature of 25°C and Abnormality the lowest obtained at a Temperature of 37°C. For Normality the Highest obtained at a temperature of 37°C and the lowest obtained at a temperature of 25°C. Key words : Artificial Insemination (ai), Normality, Abnormality, Spermatozoa, Bovine FH ABSTRAK Keberhasilan Inseminasi Buatan (IB) tergantung dari kualitas semen yang akan digunakan untuk teknik Inseminasi Buatan, dan kualitas semen dilihat dari normalitas dan abnormalitas spermatozoa.. Dari hasil yang didapatkan dalam penelitian ini yaitu Abnormalitas spermatozoa sapi FH untuk lama waktu thawing tidak berbeda nyata (P˃0,05), dengan nilai rata rata presentase abnormalitas tertinggi 4,25 ± 0,354 didapatkan pada lama waktu 40 detik, dan rata rata nilai presentase terendah didapatkan 1,75 ± 0,354 dengan lama waktu 20 detik. Lama waktu thawing terhadap normalitas spermatozoa sapi FH tidak berbeda nyata (P˃0,05), hal tersebut dapat dilihat dari nilai rata rata presentase normalitas tertinggi didapatkan 98,3 ± 0,354 dengan lama waktu 20 detik. Presentase normalitas terendah didapatkan nilai rata rata presentase 96,0 ± 0,707 dengan lama waktu 20 detik. Tetapi suhu yang didapatkan pada keduanya pada Abnormalitas dan Normalitas berpengaruh nyata (P˂0,05). Hal tersebut bisa dilihat hasil Abnormalitas yang tinggi di dapatkan pada Suhu 25°C dan Abnormalitas terendah didapatkan pada Suhu 37°C. Untuk NormalitasTertinggi didapatkan pada suhu 37°C dan terendah didapatkan pada suhu 25°C. Kata Kunci : Inseminasi Buatan (IB), Normalitas, Abnormalitas, Spermatozoa, Sapi FH
Kajian Bioprospeksi Daun Gatal (Laportea decumana), Kemaduh (Dendrocnide stimulans (L.f) Chew) dan Bedor (Girardinia palmata Chew) di Desa Gubuk Klakah dan Desa Ngadas Kecamatan Poncokusumo Kabupaten Malang Ariski Vevi Liswandari; Nour Athiroh AS; Ratna Juniwati Lisminingsih
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 2 (2021): Memperjelas Fenomena
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Stingging nettle leaves are one of the plants that are usually found by the community, but most people do not know the benefits and uses and so gatel leaves are rarely used by people in the Village of Klakah Gubuk and Desa Ngadas. This study aims to determine the bioprospection and abundance of Stingging nettle leaves Laportea decumana), kemaduh (Dendrocnide stimulant (L.f) Chew) and bedor (Girardinia palmata Gaud) by Gubuk Klakah and Ngadas villages. This research was conducted in the Village of Klakah Gubuk and Ngadas Village, Poncokusumo District, Malang Regency in May-July 2020. This research uses descriptive methods which include: literature study, field observations, interviews using questionnaires, data analysis and observation documentation. The respondents taken were 95 respondents taken purposive sampling. The results of this study indicate that the response of the community of Gubuk Klakah village and Ngadas village is high regarding the knowledge of Stingging nettle leaves plants, but the response of the community in the village of Ngadas is high regarding the utilization of the bedor (Girardinia palmata Gaud) for customary needs and natural pesticides. Keywords: stingging nettle leaves, abundance, Gubuk Klakah Village, Ngadas Village, bioprospection ABSTRAK Daun gatal merupakan tumbuhan yang biasanya dijumpai oleh masyarakat, namun sebagian besar masyarakat tidak mengetahui manfaat dan kegunaannya sehingga daun gatal jarang digunakan oleh masyarakat di Desa Gubuk Klakah dan Desa Ngadas. Penelitian ini bertujuan untuk mengetahui bioprospeksi dan kelimpahan daun gatal (Laportea decumana), kemaduh (Dendrocnide stimulans (L.f) Chew) dan bedor (Girardinia palmata Gaud) oleh masyarakat desa Gubuk Klakah dan desa Ngadas. Penelitian ini dilaksanakan di Desa Gubuk Klakah dan Desa Ngadas Kecamatan Poncokusumo Kabupaten Malang pada bulan Mei- Juli 2020. Penelitian ini menggunakan metode deskriptif yang meliputi: studi pustaka, pengamatan langsung di lapangan, wawancara dengan menggunakan kuesioner, analisis data dan dokumentasi pengamatan. Responden yang diambil adalah 95 responden yang diambil dengan teknik purposive sampling. Hasil penelitian ini menunjukkan bahwa respon masyarakat desa Gubuk Klakah dan desa Ngadas tinggi mengenai pengetahuan deskripsi tumbuhan daun gatal, namun respon masyarakat di desa Ngadas tinggi mengenai pemanfaatan tentang tumbuhan bedor (Girardinia palmata Gaud) untuk keperluan adat dan pestisida alami. Kata kunci: daun gatal, kelimpahan, Desa Gubuk Klakah, Desa Ngadas, bioprospeksi
Prevalansi Malaria di Puskesmas Sanggeng Kabupaten Manokwari Periode November sampai Desember 2019 Deasy Erawati; Febriza Dwiranti; Rina Anita Mogea
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 2 (2021): Memperjelas Fenomena
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Malaria is a disease caused by parasitic infection, named Protozoa from the genus Plasmodium which is transmitted to humans by the bite of Anopheles mosquito. Manokwari Regency, which is located in West Papua Province, is a high malaria endemic area with Annual Parasite Incidence (API) 22.88 in 2018, this numbers is included in the High Case Incidence (HCI)> 5 category. The aim of this study is to analyze malaria cases in patients who treated at Sanggeng Public Health Center from November to December 2019 based on; the number of cases, patient characteristics (age group and gender) and type of Plasmodium. This research method is descriptive with a laboratory approach, namely microscopic examination of thin and thick blood preparations using a microscope. The results of the study of 730 patients, there were 35 malaria positive blood supplies. The highest prevalence of people with malaria were aged ≥ 15 years (51.42%). Most of the patients with malaria based on gender were women (51.43%) and the types of plasmodium found were Plasmodium falciparum (20%) and Plasmodium vivax (80%). Keywords: Plasmodium falciparum, Plasmodium vivax, Anopheles, malaria endemic ABSTRAK Malaria merupakan penyakit yang disebabkan infeksi parasit yaitu Protozoa dari genus Plasmodium yang ditular pada manusia oleh gigitan nyamuk Anopheles. Kabupaten Manokwari yang berada di wilayah Provinsi Papua Barat merupakan daerah endemis tinggi malaria dengan Annual Parasite Incidence (API) 22,88 tahun 2018, angka ini termasuk dalam kategori High Case Incidence (HCI) > 5. Tujuan penelitian ini yaitu menganalisis kasus malaria pada pasien yang berobat di Puskemas Sanggeng dari bulan November sampai Desember 2019 . Berdasarkan jumlah kasus, karakteristik pasien (berdasarkan kelompok umur dan jenis kelamin) dan jenis Plasmodium. Metode penelitian ini adalah deskriptif dengan pendekatan laboratorium yaitu pemeriksaan secara mikroskopik sediaan darah tipis dan sediaan darah tebal menggunakan mikroskop. Hasil penelitian dari 730 pasien terdapat 35 sedian darah positif malaria. Prevalensi usia yang kena malaria paling tinggi pada usia ≥ 15 tahun (51,42 %). Penderita malaria berdasarkan jenis kelamin terbanyak adalah perempuan (51,43 %) dan jenis plasmodium yang ditemukan adalah Plasmodium falciparum (20%) dan Plasmodium vivax (80%). Kata kunci: Plasmodium falciparum, Plasmodium vivax, Anopheles, endemis malaria
Potensi Ekstrak Daun Jamblang (Syzigium cumini L.) Sebagai Larvasida Untuk Pengendalian Larva Nyamuk Aedes aegypti Sofia Ery Rahayu; Agus Dharmawan; Vivi Ary Lindya Putri
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 2 (2021): Memperjelas Fenomena
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

The leaves of jamblang (Syzygium cumini L.) are known to contain active compounds including flavonoids, tannins, alkaloids, triterpenoids, monoterpenens, and essensial oil. The various concentrations of jamblang leaves extract are expected to affect the mortality of 3rd instar Aedes aegypti mosquitoes larvae. The purpose of this reseacrh was to identify the active compound of jamblang leaves and determine the effective concentration of LC50 of jamblang leaves methanol extract to affect the mortality of 3rd instar A. aegypti mosquitoes larvae. This research used parts of young leaves, mixed leaves (young leaves and old leaved), and old leaves with a concentration of 0 ppm, 250 ppm, 500 ppm, 750 ppm, 1000 ppm and three replications. Observations were made for 96 hours the mortality of 3rd instar A. aegypti mosquitoes larvae. The result showed that extract concentration became higher, the mortality of 3rd instar A. aegypti mosquitoes larvae will be higher too. The higher average mortality was found in the extract of old jamblang leaves with s concentration of 1000 ppm and the result of probit analysis showed that the LC50 value which effectively killed 3rd instar A. aegypti mosquitoes larvae was the old leaves extract with a concentration of 733 ppm. Key words: Aedes aegypti, larvicide, mortality, Syzygium cumini L. ABSTRAK Daun jamblang (Syzygium cumini L.) diketahui mengandung senyawa aktif antara lain flavonoid, tanin, alkaloid, triterpenoid, monoterpen, dan minyak atsiri. Macam konsentrasi ekstrak daun jamblang diharapkan berpengaruh terhadap mortalitas nyamuk Aedes aegypti instar III. Tujuan dari penelitian ini adalah untuk mengidentifikasi senyawa aktif daun jamblang dan menentukan konsentrasi efektif LC50 ekstrak metanol daun jamblang terhadap mortalitas larva nyamuk Aedes aegypti instar III.. Penelitian ini menggunakan bagian daun muda, daun campuran (daun muda dan daun tua), dan daun tua dengan konsentrasi 0 ppm, 250 ppm, 500 ppm, 750 ppm, 1000 ppm dan tiga kali ulangan. Pengamatan dilakukan selama 96 jam terhadap mortalitas larva A. aegypti instar III. Hasil penelitian menunjukkan bahwa semakin tinggi konsentrasi ekstrak, maka semakin tinggi pula mortalitas larva nyamuk A. aegypti instar III. Hasil rerata mortalitas tertinggi terjadi pada ekstrak daun jamblang tua dengan konsentrasi 1000 ppm dan hasil analisa probit menunjukkan nilai LC50 yang efektif membunuh larva nyamuk A. aegypti yaitu ekstrak daun tua dengan konsentrasi 733 ppm. Kata Kunci: Aedes aegypti, larvasida, mortalitas, Syzygium cumini L.
Keanekaragaman Belalang (Orthoptera: Acrididae) padaEkosistem Sawah di Desa Banyuasin Kecamatan Riau Silip Kabupaten bangka Riko Irwanto; Tissya Milly Gusnia
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 2 (2021): Memperjelas Fenomena
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

The paddy field ecosystem is a place for interactions between plants and animals, especially between rice plants and grasshoppers. Grasshoppers act as eaters of rice plants, of course, become a threat to agriculture so that they can reduce agricultural productivity. This study aims to determine the diversity of Orthoptera: Acrididae in the rice fields of Banyuasin Village, Riau Silip District, Bangka Regency and the habitat conditions of Orthoptera: Acrididae in the rice fields of Banyuasin Village, Riau Silip District, Bangka Regency. This research is a descriptive quantitative research using purposive sampling method using insect net and handsorting techniques. Measurement of environmental factors is carried out by taking data on soil temperature, soil moisture, and light intensity. The research data were analyzed using the Shannon diversity index and the evenness index. Based on observations, there were 6 types of Acrididae family found in the rice field ecosystem, namely 16 individuals of Valanga nigricornis, 12 of Xenocatantops humilis, 11 of Phlaeoba fumosa, 13 Traulia azureipennis, 9 Oxya hyla and 3 Trimerotropis pallidipenni. The analysis showed that the diversity index of grasshoppers was 1.70 which was classified as moderate criteria and the evenness index was 0.41 which was classified as moderate. The locust habitat that has been obtained has optimal soil temperatures ranging from 35oC-45oC, with 50% soil moisture. Keywords: Acrididae, diversity, Ecosystem, Orthoptera, Rice Fields ABSTRAK Ekosistem sawah merupakan tempat terjadinya interaksi antara tumbuhan dan hewan khususnya antara tumbuhan padi dan Belalang. Belalang bertindak sebagai pemakan tumbuhan padi tentunya menjadi ancaman bagi pertanian sehingga dapat menurunkan produktivitas pertanian. Penelitian ini bertujuan untuk mengetahui keanekaragaman Orthoptera:Acrididae di ekosistem sawah Desa Banyuasin Kecamatan Riau Silip Kabupaten Bangka dan kondisi habitat Orthoptera:Acrididae di ekosistem sawah Desa Banyuasin Kecamatan Riau Silip Kabupaten Bangka. Penelitian ini berupa deskriptif kuantitatif dengan menggunakan metode purposive sampling dengan menggunakan tehnik insect net dan handsorting. Pengukuran faktor lingkungan dilakukan dengan mengambil data suhu tanah, kelembaban tanah, dan intensitas cahaya. Data hasil penelitian dianalisis dengan menggunakan indeks keanekaragaman Shanon, dan indeks kemerataan. Berdasarkan hasil pengamatan, didapatkan sebanyak 6 jenis family Acrididae yang terdapat pada ekosistem sawah, yaitu Valanga nigricornis sebanyak 16 individu, Xenocatantops humilis berjumlah 12 individu, Phlaeoba fumosa berjumlah 11 individu, Traulia azureipennis 13 individu, Oxya hyla berjumlah 9 individu dan Trimerotropis pallidipennis berjumlah 3 individu. Hasil analisis menunjukkan bahwa indeks keanekaragaman belalang sebesar 1,70 tergolong kriteria sedang dan indeks kemerataan sebesar 0,41 tergolong sedang. Habitat belalang yang telah didapatkan ini memiliki suhu tanah yang optimal mulai dari 35oC-45oC, dengan kelembaban tanah 50%. Kata kunci: Acrididae, Ekosistem, keanekaragaman, Orthoptera, Sawah
Perbandingan Kuantitas dan Kualitas DNA Bacillus sp. antara Heat Treatment dan Filter berbasis Kit Durrotun Ni'mah; Muhammad Zainul Fadli; Rio Risandiansyah
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 2 (2021): Memperjelas Fenomena
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Diagnosis of bacterial infection can be done quickly with simplified DNA isolation methods, such as Heat Treatment, which is a simple and inexpensive DNA isolation method, but detection and quantification levels of Bacillus sp. still unknown. This study aims to compare the quantity and quality of DNA isolates from Bacillus sp. using Heat Treatment etraction method and Filter Based isolation method. This research used in vitro experiment method. Heat Treatment is compared to Filter Based Kit according to Limit of Detection (LoD) and Limit of Quantification (LoQ). Data analyzed with ANOVA and Honesty Significant Difference (HSD). DNA yield obtained by Heat Treatment at the highest concentration was 5 µg/ml, whereas Filter Based Kit was 3.67 µg/ml. LoD of the Heat treatment and Filter Based Kit were 1.61 and 5.72 µg/ml, while LoQ of the Heat treatment and Filter Based Kit were 4.87 and 17.34 µg/ml. The number of bacteria that exceeded the LoD and LoQ of Heat Treatment was 101 and 104 CFU/ml, whereas the LoD and LoQ of Filter Based Kit was >104 CFU/ml. The quantitiy of Bacillus sp. DNA with Heat Treatment method is better than Filter Based Kit. The quality of Bacillus sp. DNA both the Heat Treatment and Filter Based Kit methods are not good. Keywords: DNA isolation, Bacillus sp., heat treatment, Filter Based Kit ABSTRAK Penegakkan diagnosis infeksi bakteri dapat dilakukan secara cepat dengan metode isolasi DNA yang telah disederhanakan, seperti Heat Treatment yang merupakan metode ekstraksi DNA sederhana dan ekonomis, namun tingkat deteksi dan kuantifikasi terhadap Bacillus sp. masih belum diketahui. Penelitian ini bertujuan membandingkan kuantitas dan kualitas DNA dari Bacillus sp. dengan menggunakan metode ekstraksi Heat Treatment dan metode isolasi Filter Based Kit. Penelitian ini menggunakan metode penelitian eksperimen secara in vitro. Heat Treatment dibandingkan dengan Filter Based Kit berdasarkan Limit of Detection (LoD) and Limit of Quantification (LoQ). Analisa data menggunakan uji ANOVA serta uji Honesty Significant Diference (HSD). Yield DNA yang didapatkan Heat Treatment pada konsentrasi terbesar adalah 5 µg/ml, sedangkan dengan Filter Based Kit adalah 3.67 µg/ml. Nilai LoD Heat treatment dan Filter Based Kit adalah 1.61 µg/ml dan 5.72 µg/ml, sedangkan nilai LoQ Heat treatment dan Filter Based Kit adalah 4.87 µg/ml dan 17.34 µg/ml. Jumlah bakteri yang melebihi nilai LoD dan LoQ Heat treatment adalah 101 CFU/ml dan 104 CFU/ml, sementara LoD dan LoQ Filter Based Kit adalah >104 CFU/ml. Kuantitas DNA Bacillus sp. dengan metode Heat Treatment lebih baik dibandingkan dengan Filter Based Kit. Kualitas DNA Bacillus sp. baik dengan metode Heat Treatment maupun Filter Based Kit tidak baik. Kata kunci: isolasi DNA, Bacillus sp., heat treatment, Filter Based Kit
Efek Penambahan Bungkil Kedelai pada Pakan terhadap Pertambahan Berat Kelinci (Oryctolagus cuniculus) Agus Dharmawan; Abdul Gofur; Dania Merit Novitasari
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 6 No 2 (2021): Memperjelas Fenomena
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Animal feed can be obtained through the use of waste as a component of feed ingredients to meet nutritional needs, which is by using soybean meal in feed ingredients. The study aims to determine the effect of adding soybean meal to feed on rabbit weight gain as well as the composition of feed which is effective and efficient. The experimental research was arranged in a Completely Randomized Design with four treatments namely, control (P0) in the form of Pap Milk (SP), 8% soybean meal (P1), 10% soybean meal (P2), and 12% soybean meal (P3) with four replications. Data analysis using One Way Anova with Post Hoc Duncan Test. The results showed the differences in weight gain of rabbits in the four treatments of adding soybean meal to the feed. The result of statistical analysis of rabbit weight gain at the control treatment (P0), soybean meal 8% (P1), and soybean meal 10% (P2) were not significantly different. The treatment of 12% soybean meal (P3) showed the lowest weight gain. The treatment of 10% soybean meal (P2) showed the highest weight gain of rabbits with the lowest feed conversion value. So that the treatment of 10% soybean meal (P2) is an effective and efficient feed formula to increase the weight of rabbits. Keywords: Soybean Meal, rabbit, rabbit weight gain ABSTRAK Pakan ternak didapatkan melalui pemanfaatan limbah sebagai komponen bahan pakan untuk memenuhi nutrisi yang dibutuhkan, salah satunya dengan memanfaatan bungkil kedelai sebagai bahan pakan. Penelitian bertujuan untuk mengetahui efek penambahan bungkil kedelai pada pakan terhadap pertambahan berat kelinci serta formula pakan yang efektif dan efisien. Penelitian eksperimental disusun secara Rancangan Acak Lengkap dengan empat perlakuan yaitu, kontrol (P0) berupa Susu Pap (SP), bungkil kedelai 8% (P1), bungkil kedelai 10% (P2) dan bungkil kedelai 12% (P3) dengan empat ulangan. Data dianalisis menggunakan One Way Anova dengan Uji Lanjut Post Hoc. Hasil penelitian menunjukkan terdapat perbedaan pertambahan berat kelinci pada empat perlakuan penambahan bungkil kedelai pada pakan. Hasil analisis statistik pertambahan berat kelinci pada perlakuan kontrol (P0), bungkil kedelai 8% (P1), dan bungkil kedelai 10% (P2) tidak berbeda nyata. Perlakuan P3 menunjukkan pertambahan berat kelinci terendah. Perlakuan bungkil kedelai 10% (P2) menunjukkan pertambahan berat kelinci tertinggi dengan nilai konversi pakan terendah. Sehingga perlakuan bungkil kedelai 10% (P2) merupakan formula pakan yang efektif dan efisien untuk menaikkan berat kelinci. Kata kunci: Bungkil kedelai, kelinci, pertambahan berat kelinci
Uji Toksisitas Subkronik 28 Hari Kombinasi Ekstrak Metanolik Benalu Teh dan Benalu Mangga Terhadap Profil Protein pada Tikus Wistar (Rattus norvegicus) Betina Umu Intan Kinasih; Nour Athiroh Abdoes Sjakoer; Nurul Jadid Mubarakati
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

There are 3 indicators for indicating a protein breakdown in the blood through a total protein, albumin, and globulin stability. A compound capable of acting as antioxidants is needed to stabilize the protein's condition. This research is aimed at identifying the toxic effects of extract’s combination from tea parasite and mango parasite on the conditions of blood’s protein by giving EMBTBM constantly for 28 days. The data analysis used ANOVA one-way by SPSS version 17.0. As for the animal test used was a female Wistar rat of 20 with four treatments are control, treatment 1 with a dose of 250mg/Kg body weight (BW). Treatment 2 with a dose of 500mg/Kg body weight (BW) and treatment 3 with a dose of 1000mg/Kg body weight (BW). The result of this research shows that there is no difference between treatment and control over the results of a total protein and albumin. But, at the result of globulin, there is a difference between treatment and control. Therefore, EMBTBM is safe for profile protein and does not cause toxicity because protein is stable and not over-reducing. Keywords:Subchronic,Total Proteins, Albumine, Globuline, Toxicity