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Jurnal Ilmiah Biosaintropis (Bioscience-Tropic)
Published by Universitas Islam Malang
ISSN : 23382805     EISSN : 24609455     DOI : https://doi.org/10.33474/e-jbst.v7i2.305
Core Subject : Agriculture, Social,
Agriculture, Biological Sciences & Forestry Environmental Science
Jurnal ini mengkaji fenomena dan temuan penelitian di bidang biologi dan ilmu-ilmu dasar (sains) lainnya serta bidang studi di wilayah tropis. Jurnal ini ditujukan untuk menemukan solusi alternatif dalam perkembangan ilmu biologi demi kesejahteraan masyarakat Indonesia dan Dunia.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Bioteknologi
Articles 270 Documents
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Struktur Makroinvertebrata Bentos Sebagai Bioindikator Kualitas Air di Kawasan Wisata Coban Talun, Kota Batu - Jawa Timur Apria Ningsih; Husain Latuconsina; Hasan Zayadi
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Makroinvertebrata bentos merupakan hewan yang memiliki kepekaan terhadap perubahan lingkungan, sehingga dapat dijadikan sebagai bioindikator untuk menentukan kualitas suatu perairan. Tujuan penelitian ini adalah untuk menginventarisasi makroinvertebrata bentos dan mengetahui status perairan di Kawasan Wisata Coban Talun, Kota Batu-Jawa Timur. Penelitian ini dilaksanakan pada bulan Oktober 2019 hingga Januari 2020. Pengambilan sampel penelitian mengunakan metode Purposive sampling. Makroinvertebrata dikumpulkan dari tiga lokasi (stasiun satu dengan karakteristik daerah wisata dan penambangan pasir, stasiun dua daerah konversi bendungan dan aktivitas wisata, stasiun daerah wisata dan aktivitas merumput) dengan mengunakan Surber net dan hand net. Kualitas periaran berdasarkan struktur komunitas makroinvertebrata bentos dianalisis mengunakan indeks keanekaragaman, Family Biotic Index (FBI) dan Biological Monitoring Working Party-Average Score Per Taxon (BMWP-ASPT). Hasil penelitian mendapatkan 9 taksa dari ketiga stasiun yaitu Coleoptera, Diptera, Ephemeroptera, Mollusca, Odonata Plecoptera, Thricoptera ,Tricadida dan Rhyanchobdellida. Indek keanekaragaman dari ketiga stasiun memiliki nilai 2.4 – 2.58, yang menunjukan keanekragaman sedang dengan status perairan mengalami pencemaran ringan. Kualitas air dari semua stasiun menujukan cukup hingga cukup tercemar (berdasarkan FBI) dan kualiatas perairan tercemar ringan (berdasarkan BMPW-ASPT). Berdasarkan profil struktur komunitas makroinvertebrata bentos kualitas perairan pada kawasan wisata Coban Talun, memiliki kualitas tercemar ringan hingga sedang. Sehingga diperlukan penggelolaan daerah aliran sungai secara berkelanjuatan untuk menjaga kualitas perairan. Kata kunci: Makroinvertebrata bentos, Kualitas perairan, Coban talun, Batu- Jawa Timur Makroinvertebrata bentos merupakan hewan yang memiliki kepekaan terhadap perubahan lingkungan, sehingga dapat dijadikan sebagai bioindikator untuk menentukan kualitas suatu perairan. Tujuan penelitian ini adalah untuk menginventarisasi makroinvertebrata bentos dan mengetahui status perairan di Kawasan Wisata Coban Talun, Kota Batu-Jawa Timur. Penelitian ini dilaksanakan pada bulan Oktober 2019 hingga Januari 2020. Pengambilan sampel penelitian mengunakan metode Purposive sampling. Makroinvertebrata dikumpulkan dari tiga lokasi (stasiun satu dengan karakteristik daerah wisata dan penambangan pasir, stasiun dua daerah konversi bendungan dan aktivitas wisata, stasiun daerah wisata dan aktivitas merumput) dengan mengunakan Surber net dan hand net. Kualitas periaran berdasarkan struktur komunitas makroinvertebrata bentos dianalisis mengunakan indeks keanekaragaman, Family Biotic Index (FBI) dan Biological Monitoring Working Party-Average Score Per Taxon (BMWP-ASPT). Hasil penelitian mendapatkan 9 taksa dari ketiga stasiun yaitu Coleoptera, Diptera, Ephemeroptera, Mollusca, Odonata Plecoptera, Thricoptera ,Tricadida dan Rhyanchobdellida. Indek keanekaragaman dari ketiga stasiun memiliki nilai 2.4 – 2.58, yang menunjukan keanekragaman sedang dengan status perairan mengalami pencemaran ringan. Kualitas air dari semua stasiun menujukan cukup hingga cukup tercemar (berdasarkan FBI) dan kualiatas perairan tercemar ringan (berdasarkan BMPW-ASPT). Berdasarkan profil struktur komunitas makroinvertebrata bentos kualitas perairan pada kawasan wisata Coban Talun, memiliki kualitas tercemar ringan hingga sedang. Sehingga diperlukan penggelolaan daerah aliran sungai secara berkelanjuatan untuk menjaga kualitas perairan. Kata kunci: Makroinvertebrata bentos, Kualitas perairan, Coban talun, Batu- Jawa Timur
Uji Bakteri Coliform dan (Escherichia coli) pada Air Tercemar Dengan Penggunaan Susunan Filter Linda Purwanti; Ahmad Syauqi; Saimul Laili
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Clean water is a serious problem in Indonesia, the availability of clean water has shrunk drastically due to deteriorating environment and pollution. Drinking water that meets safety standards is increasingly scarce. More than 100 million people need safe water sources. Filter media is able to remove chemicals in water such as turbid, odor, oily, yellowish, rusty and muddy making it unfit for consumption. In addition, the purpose of the clarification results must also be considered whether to drink household needs or other needs. This study uses Lauryl Tryptose Broth (LTB) media and petrifilm media. Petrifilm results show that it is very easy to count the bacteria Colifrom and Escerichia coli using petrifilm media. From the analysis of calculations using the Paired Samples T-Test on the sample before filtering and after filtering it was obtained the Tcount value of 2.28 with a P value 0.015 which showed very successful or significant results between the samples before and after the filter. In this analysis have normally distributed data with a significant value of P = 0.015 (<0.05). In this case the Paired Samples T-Test was tested for normality (Shapiro-Wilk) and the results were obtained in the form of a P value of <.001 which would indicate that the data was normally distributed, indicated by a P value <0.05. The filter on this filter is very useful for filtering on Escerichia coli bacteria because this filter removes the bacteria, but Colifrom bacteria can still escape the use of the filter, because it depends on environmental conditions. Colifrom and Escerichia coli bacteria occur is seen from the relationship between sources of pollution such as, for example, such as septic tanks, distance of wells with waste, trash, and livestock pollution. The quality of drinking water is very close to the presence of Colifrom and Escerichia coli bacteria which are likely to have pathogenic bacteria that are harmful to health in the quality of well water. Water samples to be observed using well water using 2 treatments as many as 12 repetitions. Thus the total sample obtained as many as 24 water samples will be observed. While the test making of Lauryl Triptose Broth medium was observed by incubating 48 hours at 35oC + gas. Before making the test tube medium must be sterilized first. Subsequent tests were observed with a petrifilm medium to determine the presence or absence of Colifrom and Escherichia coli bacteria by looking at the color of the battery being sought. Keywords: Drinking Water, Coliform Bacteria and Escherichia coli
Ekstraksi Astaxanthin Kulit Udang (Litopenaeus vannamei) Pantai Gunung Kidul Menggunakan Pelarut Minyak Bunga Matahari dan Etanol Anik Prasetyaningsih; Graciela Carina Najoan; Abner Wisaksono; Djoko Rahardjo
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

ABSTRACT As a maritime country with vast waters, Indonesia has many opportunities to utilize marine resources as a source of bioactive compounds that have the potential as active medicinal ingredients. One of the marine biotas that potentially contains the active compounds is the Vannamei shrimp's shell (Litopenaeus vannamei), which is commonly found as waste along the coast of Gunungkidul, Yogyakarta. The shrimp’s shell contains astaxanthin, a potential source of antioxidants for the health industry. The purpose of this study was to compare the astaxanthin extraction yield from L. vannamei shrimp shells using sunflower oil and 70% ethanol. The Astaxanthin extraction used sunflower oil and ethanol 70% as solvents and was done by maceration method, while the phytochemical test and Astaxanthin profiling used Thin Layer Chromatography and Spectrophotometer with Kelly and Harmon (1972) [5] calculations as well as pure Astaxanthin standards. The extraction yield of the 70% ethanol extraction was further processed by column chromatography using ether: ethanol (8: 2) as mobile phase. The highest Astaxanthin yield (220 mg / g of shrimp powder) was obtained from the extraction with sunflower oil compared to the 70% ethanol solvent, while the fractionation result with a chromatographic column from a crude extract of ethanol 70% showed high astaxanthin yield of 220.77 mg. / g fraction. The results of the fraction test on rat neutrophils, the best percentage reduction was at a concentration of 150 mg / g bw of rats.
Pengaruh Penambahan Tepung Biji Durian pada Pembuatan Bioplastik Cory Dian Alfarisi; Yulia Fitri; Detria Khaerun Nisa
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Plastic is a type of packaging that is often used in everyday life, however, in general, the plastic used is synthetic plastic which takes a very long time to decompose. Bioplastic is an environmentally friendly plastic film made from organic materials so that it can decompose naturally with the help of microorganisms. This study aims to determine the effects which have the potential as a supporting material in increasing bioplastic characteristics such as thickness, water absorption and biodegradation. The research method was to use experiment and analysis of varian. Durian seed flour is carried out through a process of sorting, washing, stripping, soaking, slicing, drying, packing and storing. Meanwhile, the method of making bioplastics is done by mixing tapioca flour, cornstarch, durian seed flour of adding various durian seed flour: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 grams, distilled water, acetic acid and glycerin. The analysis results obtained on the test of bioplastic characteristics, namely, thickness test ranging from 0.63 mm - 0.84 mm and water resistance test ranging from 71,43%-86,54% which water absorption test ranging from 13.46% - 28.57% and the biodegradability test ranging from 17.78% - 47 , 06%. Keywords: durian seeds, bioplastics, biodegradability, flour ABSTRAK Plastik adalah jenis pengemas yang sering dimanfaatkan dalam kehidupan sehari-hari, akan tetapi pada umumnya plastik yang digunakan merupakan plastik sintetik yang membutuhkan waktu yang sangat lama untuk terurai. Bioplastik merupakan film plastik yang ramah lingkungan yang terbuat dari bahan-bahan organik sehingga dapat terurai secara alami dengan bantuan mikroorganisme. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan tepung biji durian yang berpotensi sebagai bahan penunjang dalam meningkatkan karakteristik bioplastik seperti ketebalan, ketahanan terhadap air dan biodegradasi. Metode penelitian adalah menggunakan eksperimen dan sidik ragam data. Tepung biji durian dilakukan melalui proses penyortiran, pencucian, pengupasan, perendaman, pengirisan, pengeringan, penepungan dan penyimpanan. Sedangkan untuk metode pembuatan bioplastik dilakukan dengan mencampurkan tepung tapioka, tepung maizena, tepung biji durian dengan variasi: 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 gram, akuades, asam asetat dan gliserin. Hasil analisa yang didapat pada pengujian karakteristik bioplastik yaitu, uji ketebalan yang berkisar 0,63 mm – 0,84 mm, uji ketahanan terhadap air yang berkisar 71,43%-86,54% dengan penyerapan air berkisar 13,46 % - 28,57% dan uji biodegradibilitas yang berkisar 17,78% - 47,06%. Kata kunci: biji durian, bioplastik, biodegradibilitas, tepung.
Antidiabetes Serbuk Umbi Tanaman Yakon (Smallanthus sonchifolius) pada Kadar Gula Darah Tikus Putih (Rattus norvegicus) Thatit Nurmawati; Yeni Kartika Sari; Fina Ayu Ningtyas
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

The routine and long-term use of oral antidiabetic drugs in DM patients can make the public's perception worried about the side effects of the drug. In the Yakon (SS) plant tuber there is Fructooligosacaraide (FOS) which can reduce blood sugar levels. The research objective was to determine the effect of Yakon plant tubers on blood sugar levels in white rats with type 2 diabetes mellitus model. This type of research was experimental with a pre-post test design with control-group design. Independent variable, administration of Yakon plant tuber powder, dependent variable, blood glucose levels. The sample was divided into 2 groups (dose 1, dose 2) and a control group, each of 6 rats. The mice were given a high carbohydrate diet for 9 weeks. Treatment dose 1 (100 mg / kgBB) for 3 days. Measurement of blood sugar levels using Gluco-Dr. Data analysis used Paired Sample T-test, Independent Sample T-test, and One Way Anova. The results showed that the rats in the treatment group dose 1 experienced a decrease in the average blood sugar level of 122.83 mg / dl (p 0.002) (p 0.009). This shows that there is an effect of using Yakon plant tuber powder on blood sugar levels. The results of this study were the control group and the treatment group dose 1 (Sig. (2-tailed) = 0.000, α = 0.05). Meanwhile, the comparison results showed that there was a difference between the control group and the treatment group at dose 1, Based on the above research, Yakon tubers can be used to reduce blood sugar levels.
Studi Nilai Etnobotani dan Sosio-Ekologi Generasi Milenial Sumba Yuswa Istikomayanti; Zuni Mitasari
BIOSAINTROPIS (BIOSCIENCE-TROPIC) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (689.471 KB) | DOI: 10.33474/e-jbst.v7i1.394

Abstract

Ethnic and natural environments coexist together. However, along with the development of modernization to various corners of the region also makes challenges for indigenous peoples to remain in the future. The purpose of this study is to describe ethnobotany values in the Sumba millennial generation and to examine the socio-ecological aspects as a new perspective in ethnobotany studies. Sumba millennials are students of the Tribhuwana Tunggadewi University who are regional children who still live with a cultural environment, especially the Sumba area, East Nusa Tenggara. Through questionnaires, open questions and in-depth interviews with key respondents, it can be assessed the extent to which cultural values exist in the Sumba millennial generation who have received education and modern life. Socio-ecological studies are important to be carried out as a perspective in making various policy directions. Particularly in the direction of education, selection, and determination of customary values and how efforts to conserve them become co-existent ethnicities and environments. The results obtained by most of the millennial generation still hold customary values in their daily behavior (48%). This aspect is reflected in the use of traditional medicine, akar kayu/Linno, Psidium guajava, Annona muricata, Zingiber officinale, Curcuma zedoaria, Swietenia mahagoni, Piper betle, Cocos nucifera, Persea americana, Chromolaena odorata, Muntingia calabura, Ocimum basilicum, Coffea Sp., Anredera cordifolia, Phaleria macrocarpa. Skills in weaving, natural coloring, eating betel-nut, and positive perceptions (48%) of the preservation of its culture in the future.
Pengaruh Ekstrak Daun Kopi Robusta (Coffea canephora Pierre ex A. Froehner) terhadap Kualitas Spermatozoa Mencit (Mus musculus L.) Yang Terpapar Asap Rokok. I Komang Alit Saputra; Ni Gusti Ayu Manik Ermayanti; A.A. S. A. Sukmaningsih
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

This study aims to determine the effect of given robusta coffee (Coffea canephora Pierre ex A. Froehner) leaf extract on the spermatozoa quality of mice (Mus musculus L.) exposed to cigarette smoke. The experiment was using a completely randomized design (CRD) with five treatments and each consisted of six mice as replications. Each treatment was K- (without exposure to cigarette smoke), K+ (exposure to cigarette smoke + 0.5% Na-CMC), P1 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 60 mg/kgbw), P2 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 120 mg/kgbw) and P3 (exposure to cigarette smoke + Robusta coffee leaf extract at a dose of 180 mg/kgbw). On the 36th day, mice were taken down by anesthesia and followed by surgery to collect spermatozoa. The parameters for this observation were the concentration, motility, viability, morphology, and integrity of the spermatozoa membrane. The data obtained were analyzed statistically with One Way Anova and further tested with DMRT. The results showed that the treatment of robusta coffee leaf exstract in mice exposed to cigarette smoke had a significant effect (P<0,05) on the quality of spermatozoa.
Potensi Murraya keonigii sebagai Herbisida Alami Lussana Rossita Dewi; Praptining Rahayu
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Various kinds of agricultural products are needed to support organic farms to produce superior quality products, one of it was natural herbicides. Some plants have the potential to become natural herbicides by producing chemical compounds, namely allelopathy which can inhibit the growth of surrounding plants and Murraya keonigii is one of it. This study aims to see the potential of M. keonigii to become a natural herbicide. Grass seeds were given M keonigii’s extract at a dose of 0 gr / l (control / M0), 50 gr / lt (M1), 60 gr / lt (M2), and 70 gr / lt (M3). The results showed that the aromatic or allelopathic compounds in M ​​keonigii affected the germination. Germination was slower at doses of 60 gr / l (M2) and 70 gr / lt (M3) compared to controls (M0) and 50 gr / lt (M1). Radicle length did not increase rapidly in M2 and M3 treatment. Germination power of grass seeds in control and M0 had a value of 100% or all germinated, while M1 and M2 had 67% germination. The higher the dose of M keonigii extract, the slower the process of grass seed germination.
Distribusi dan Frekuensi Alel Golongan Darah Sistem ABO dan Rhesus pada Penduduk Pulau Gili Ketapang Probolinggo Kirana Eka Rezki; Rike Oktarianti; Hidayat Teguh Wiyono; Purwatiningsih Purwatiningsih
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

Sistem penggolongan darah pada manusia yang banyak dikenal adalah sistem ABO dan rhesus. Tujuan penelitian ini adalah Mengetahui distribusi golongan darah sistem ABO dan rhesus serta frekuensi alel pada populasi penduduk pulau Gili Ketapang, kabupaten Probolinggo. Pengambilan sampel dilakukan secara acak dengan cara dilakukan pengundian jumlah sampel yang diambil sebanyak 384 jiwa. Identifikasi golongan darah sistem ABO pada penelitian ini dilakukan dengan metode slide dengan prinsip antigen (aglutinogen) yang direaksikan dengan antibodi (aglutinin). Hasil penelitian menunjukkan distribusi golongan darah sistem ABO dan Rhesus O+ (46,61%), B+ (24,22%), A+ (23,18%), dan AB+ (5,99%). Frekuensi alel IA(0,16), frekuensi alel IB (0,16), frekuensi alel i (0,68), dan frekuensi alel rhesus positif (Rh+) adalah 1
Pengaruh Perbedaan Konsentrasi Emulgator terhadap Karakterisasi Fisik Sediaan Krim Ekstrak Rosella (Hibiscus sabdariffa) Ike Widyaningrum; Sasi Purwanti
Jurnal Ilmiah Biosaintropis (Bioscience -Tropic) Vol 7 No 1 (2021): Agustus 2021
Publisher : Fakultas Matematika & Ilmu Pengetahuan Alam - Universitas Islam Malang

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Abstract

The formulation and physical characterization test of rosella extract cream preparations have been carried out using various types of non-ionic emulsifier concentration ratios (combination of tween 80 and span 80). Comparison of the concentrations of tween 80 and span 80 used in this study were 2%, 3%, and 4%. The aim of this study included organoleptic test, homogeneity test, pH test, viscosity test, dispersion test, and emulsion type test. The result obtained from the physical characterization test were the organoleptic tests for the three formulas showed the same organoleptic results, namely red color, characteristic smell of rosella, and semisolid consistency. Homogeinity test shows that the formula is homogenous. The pH test showed that F1 4,29; F2 4,6; F3 5. Viscosity test showed that F1 432,4 mPas; F2 434,3 mPas; 435,3 mPas. The dispersion test was carried out without using a load of 50 g, 100 g, and 200 g. the results shown form the spreadability test of three formulas were were F1 5 cm; 5,5 cm; and 6 cm; F2 6 cm; 6,35 cm; 7,25; and 7,5 cm. F3 6,25 cm; 7,5 cm; 7,75 cm; 8 cm. The emulsion type indicated by the three formulas is oil in water. Based on these results, it can be seen that the emulsifier concentration has an effect on PH, viscosity, and spreadibility. The higher the concentration of emulsifier used, the higher pH value, viscosity, and spreadibility.

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