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INDONESIA
JURNAL BIOLOGI INDONESIA
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : 08544425     EISSN : 2338834X     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 847 Documents
 81   82   83   84   85 
BARCODING DNA PADA KOMUNITAS KELELAWAR PEMAKAN BUAH DI INDONESIA Zein, Moch Syamsul; Fitriana, Yuli Sulistya
JURNAL BIOLOGI INDONESIA Vol 11, No 1 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i1.2156

Abstract

Family Pteropodidae known as fruit bats which has ecological services as seed dispersers, pollinator, and plays important role in the forest regeneration. Identification of bat species frequently encountered problems, i.e.,  criptic morphology and behaviour. Therefore it is necessary to confirm its identity using DNA barcodes. Cytochrome c oxidase subunit I (COI) mitochondrial DNA is representative of the protein coding mitochondrial DNA and has been used extensively as a means of animal species identification. This study evaluated 141 specimens consist of 42 species and 17 genera which were collected from Java, Nusa Tenggara, Sumatra, Kalimantan, Sulawesi, Maluku, and Papua. Phylogenetic analysis using the neighbor-joining method, in which the genetic distance matrix calculation by Kimura-2 parameter models are implemented on the pairwise distance calculation in the program MEGA version 6:05. The results of the analysis showed variation intraspecific ranging from 0 to 7.9% (0.9 ±0.014%) and there were 4 species with very high intraspecific average sequence divergence, i.e., Penthetor lucasi (3.2%), Thoopterus nigrescens (3.7%), and Chironax melanocephalus (8.7 %). Average interspecific of genetic distance  of fruit bats in Indonesia was 20% (1.3-26.1%). These results produce a phylogeny tree construction to form a clearly different cohesive cluster, except in the genus Dobsonia ( D. moluccensis, D. viridis, and D. crenulata), Cynopterus (C. brachyotis C. minutus, and C. luzoniensis), and Macroglossus (M. minimus and M. sobrinus ), because this did not correspond with currently recognized species boundaries based on morphology. Keywords: DNA barcoding, COI, Pteropodidae 
PEROKSIDASI LIPID OLEH PARASETAMOL DAN EKSTRAK AIR PANAS TEH HIJAU (CAMELLIA SISNENSIS ) PADA SEL KHAMIR CANDIDA TROPICALIS YANG DI SIMPAN PADA SUHU RENDAH Julistiono, Heddy
JURNAL BIOLOGI INDONESIA Vol 8, No 1 (2012): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v8i1.3070

Abstract

ABSTRACTLipid Peroxidation by Paracetamol and Hot Water Extract of Green Tea (Camellia sinensis ) in Yeast Candida tropicalis preserved in Low Temperature. The use of C. tropicalis cell as a tool to evaluate antioxidant property of green tea to protect oxidative stress caused by paracetamol in cell level was investigated in our laboratory. Immediate availability of cell culture will significantly reduce the time of cell preparation. Low temperature preservation of cell culture is one of methods to produce cell cultures. However, low temperature might affectphysiological state of the cell. In this study, effect of low temperature preservation (4 ºC, 10 days) on the oxidative response of yeast cell treated with paracetamol and hot water extract of green tea had been investigated. Cells incubation with paracetamol 0.3 % for 2 h caused oxidative stress in both fresh and preserved culture since the content of a marker of oxidativestress, peroxyd lipids increased significantly. Whereas, concentration of peroxidised lipids in preserved cultures was lower than that of fresh culture. Increasing of peroxydized lipids followed with decreasing of cell viability in fresh culture but not in preserved culture. Green tea withconcentration of 0.1 % decreased peroxyd lipids in fresh cultures treated with paracetamol but not in preserved cultures. Green tea with concentration of 0.2 % or 0.4 % in increased peroxyd lipids in fresh cultures treated with paracetamol but not in preserved cultures. The data indicated that green tea did not show anti- or pro-oxidative effect in cultures preserved in low temperature treated with paracetamol. However, induction of super oxide dismutase (SOD), an antioxidant defense enzyme, was not observed in cell preserved in low temperature. The study revealedthat low temperature might make the cell more resistant against prooxidative properties of paracetamol.Keywords: C. tropicalis, oxidative stress, low temperature, paracetamol, green tea
GEOLOGI BATUAN DASAR GUNUNG CIREMAI JAWA BARAT Samodra, Hanang
JURNAL BIOLOGI INDONESIA Vol 4, No 5 (2008): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v4i5.3213

Abstract

ABSTRACTThe Stone Geology Structure of Ciremai Mountain West Jawa. A Middle to LateMiocene rocks association around the Ceremai Volcano, namely the Cinambo, Cantayanand Bantarujeg Formations is a deep marine sediment that physiographically belongs tothe Bogor Zone. As a submarine fan system, this turbidite sediment has already progradedto the north. The provenance of sediments is a landmass that occupied the southern partof the deep basin. Shallowing of the basin during Late Tertiary is roled by Plioceneclaystone forming of the Subang, Kaliwangu and Citalang Formations. The claystonewas deposited in a middle neritic to fluviatile environment. During the early Quaternarytime the deep basin has totally become a landmass and the building of Ceremai Volcanowas started. The difference kind of rocks will influence chemical composition of soil,and the diversity of vegetation is possibly implied by this phenomenon.Key words: stone. structure, geology, ciremai
BIOMASA MANGROVE DAN BIOTA ASOSIASI DI KAWASAN PESISIR KOTA BONTANG Yonvitner, Yonvitner; Wahyudin, Yudi; Mujio, Mujio; Trihandoyo, Arif
JURNAL BIOLOGI INDONESIA Vol 15, No 1 (2019): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v15i1.3771

Abstract

ABSTRACTMangrove is an important buffer ecosystem in the coastal area which has a function as carbon absorption through biomass growth.  Others also as support any kind ecosystem particularly is fish's biodiversity.  This research that was conducted in Bontang coast found 4 species of mangrove that dominated by Rhizophora apiculate that biomass production average 3714 kg/ha, Rhizophora mucronata 2415 kg/ha, Sonneratia 407 kg/ ha, and Bruguiera 251 kg/ha. Mangrove ecosystem support to biomass production of the shell, shrimp, mollusc until 2408 kg/ha of total population found.  The research found that have a similar trend of mangrove growth and biomass related biota production.  The message of this research is important of mangrove conservation to support biota and another ecosystem in the coastal area.    Keywords:  Mangrove, Association, Bontang, 
MODEL EUTROFIKASI 2-DIMENSI BERLAPIS UNTUK OPTIMALISASI LOKASI ZONA BUDIDAYA IKAN KARAMBA JARING APUNG (KJA) DI WADUK JATILUHUR Harsono, Eko
JURNAL BIOLOGI INDONESIA Vol 12, No 2 (2016): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v12i2.2896

Abstract

ABSTRACTThe development of floating net aquaculture in Jatiluhur reservoir that reach up 21000 to 26 717 plot have been resulted in eutrophication that interfere of water treatmen raw water, tourism and water sports, so the reduction and redeployment zones floating net in the reservoir needs to be done. The purpose of this study looking for zone of floating net aquaculture in Jatiluhur reservoir. The zone is obtained from the optimization by maximizing the density plots floating net per segment in alternate zones with constraints status trophic allowed in the area of water treatment raw water in take, tourism and water sports in the downstream Jatiluhur reservoir through simulation of the chlorophyll-a concentration distribution from the 2 -dimensional layered eutrophication model for scenarios increase every 10 plots floating net in segments of alternative zones 1, 2, 3 in the downstream reservoirs and alternate zone 4 in the middle of the reservoir. The results of the eutrophication 2-dimensional layered model calculation been obtained segment length and width of 100 m. The optimization results show, the carriying capacity of water due to zoning plots Jatiluhur reservoir floating net per segment in the middle of the reservoir is greater than the downstream of the reservoir, and to get water Jatiluhur reservoir in oligotrophic status that does not interfere with other uses of the reservoir water, and to get water Jatiluhur reservoir in oligotrophic status that does not interfere with other uses of the reservoir water, the highest density of a plot floating net per segment is the alternative zone 4 of 10 plot floating net per segment or 4,420 plot floating net. For the the aquaculture fish in floating net in the area are currently recommended to be moved to alternative zone 4 which is located in the central part Jatiluhur reservoir.Keywords: floating net plot zone, Jatiluhur reservoir, optimalitation, 2-dimensional layered eutrophication
KELIMPAHAN DAN KERAGAMAN KAPANG PADA SAMPEL TANAH DI SEKITAR KAWASAN TAMAN NASIONAL GUNUNG CIREMAI, JAWA BARAT Ilyas, Muhammad
JURNAL BIOLOGI INDONESIA Vol 5, No 3 (2009): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v5i3.3180

Abstract

ABSTRACTThe Abundance and Diversity of Fungi Inhabiting Soil Samples from Mount Ciremai NationalPark, West Java. A study on isolation and identification of mould inhabiting soil samples hasbeen conducted. The objective of the study was to isolate and identify mould inhabiting soilsamples from Mount Ciremai National Park, West Java. The mould isolation was based onsample dillution method with Rose Bengal Cloramphenicol Agar mould isolation media. Theabundance of mould counted by measuring the average Colony Forming Unit (CFU)/ml of allmould colonies which growth on isolation media by Total Plate Count (TPC) method. Thediversity of isolated mould was identified based on morphological character. The result showedthat the average of mould colonies between 8,5x103-11,3x104 CFU/ml. The result of mouldidentification showed that nine mould generas are Aspergillus (6 species), Chaetomium (2species), Eupenicillium, Gliocladium, Mucor, Penicillium (6 species), Rhizopus, Trichoderma(2 species), and two groups of unidentified sterile mould.Key words: abundance, diversity, mould, soil samples, Mount Ciremai National Park
BENTHIC MOLLUSCS COMMUNITIES IN THE INTERTIDAL COAST OF TANIMBAR ISLANDS, WEST SOUTHEAST MOLLUCAS Heryanto, Heryanto
JURNAL BIOLOGI INDONESIA Vol 14, No 1 (2018): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v14i1.3665

Abstract

ABSTRACTThis paper presents the results of a survey on intertidal molluscs in 3 habitats (Seagrass bed, coral reef, and sandflat) in Tanimbar Islands of West South-east Moluccas, Indonesia. Six relatively large and two small islands were attained to perform 125 plots of 34 stations. Molluscs? diversity in each habitat were calculated using Shannon-Wiener formulation whereas Mann-Whitney formulation for comparing between habitats. Multivariate analyses for clustering of PAST 2.17 was utilized to further the analyses. A number of 101 species gastropods (26 families) and 51 species of bivalves (19 families) were recorded. Seagrass bed was inhabited by 100 species, followed by coral reef by 85 and sandflat by 44 species. Shannon-Wiener indexes for molluscs? diversity range in seagrass was 0.39 to 1.29, in coral was 0.27 to 1.14 and in sandflat was 0.52 to 0.99. Clustering analyses for stations revealed that three stations in seagrass were the most dissimilar, followed by one station in coral due to habitat. Clustering analyses for species found four dissimilar groups of molluscs, whereas a group has many consimilar members.Keywords: Seagrass, coral, sandflat, diversity, clustering
PENANDA GENETIK TARSIUS (TARSIUS SPP.) DENGAN MENGGUNAKAN GEN CYTOCHROME OXIDASE I (COI) DNA MITOKONDRIA (MTDNA) MELALUI METODE SEKUENSING Kuswandi, Paramita Cahyaningrum; ,, Wirdateti; Wulandari, Sri Wijayanti
JURNAL BIOLOGI INDONESIA Vol 11, No 2 (2015): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v11i2.2202

Abstract

ABSTRACTTarsier (Tarsius spp.) are the smallest primates in the world. Currently there are 10 species, of which 9 species are distributed in Indonesia and 8 species of them are endemic to Sulawesi. Morphologically  the Sulawesi spesies are almost similar. This research is aimed to identify the use of Cytochrome Oxidase I (COI) gene as a genetic marker on Tarsius spp. for conservation purposes. Sixteen individuals consisted of 10 Tarsius bancanus, 4 Tarsius sp., 1 Tarsius wallacei , and 1 Tarsius sangirensis were collected from various places and analysed using COI gene. The results showed  there were 238 different sites of nucleotides and 159 sites of amino acids from the total amount of 838 bp. The genetic distance by Kimura-2 parameter showed the highest value was 26% while the lowest was 0%. The average genetic distance was 11,5%. Phylogenetic tree constructed by Neighbour-Joining method based on nucleotides sequence showed that the COI gene could be used as a genetic marker to differentiate among Tarsius spp. but could not be used as a clear marker for tarsiers in Sulawesi. Based on the analysis, there is a high value of genetic variation among Tarsius spp. with much lower genetic variation in Western Tarsier population compared to Eastern Tarsier. Keyword: Genetic marker, tarsier, COI, DNA mitochondria, conservation 
KAJIAN PAKAN BERSUMBER ENERGI TINGGI PADA PEMBENTUKKAN MONYET OBES Oktarina, Ria; Mansjoer, Sri Supraptini; Astuti, Dewi Apri; Suparto, Irma Herawati; Sajuthi, Dondin
JURNAL BIOLOGI INDONESIA Vol 6, No 3 (2010): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v6i3.3145

Abstract

ABSTRACTHigh Energy Diet to Develop Obese Animal Model in Cynomolgus Monkeys (Macacafascicularis). Obesity is a primary predisposition for diseases such as metabolic syndrome(insulin resistance, blood lipid abnormality, and hypertension), type 2 diabetes, andcardiovascular disease. The effort to overcome obesity is needed by understanding thedevelopment of obesity. Therefore, it is urgent to carry out preclinical trials by using an obeseanimal model however it is still limited. The objective of this research was to study the effect ofhigh energy diet with animal fat and soluble carbohydrate to produce obese cynomolgusmonkeys (Macaca fascicularis). Animals used in this study were 15 adult males dividedequally into three treatment groups and were given diets for 4 months. The 3 groups were 1)receiving diet A consists of beef tallow without egg yolk; 2) receiving diet B consists of beeftallow and egg yolk, (energy 4,207 cal/g, fat 19.62%, and starch 60.34% in both first two diet) 3)receiving diet C consists of monkey chow as control (energy 4,330 cal/g, fat 5.55%, and starch51.38%). Measurement were taken every four weeks for body weight, crown rump length, hipdiameter, abdominal skin thickness, body mass index (BMI), nutrient consumption anddigestibility. Results showed that animals received diet B had significant increase in bodyweight and BMI at week 4 and 8. Hip diameter and abdominal skin thickness were significantstarting at week 4 in animals receiving diet with egg yolk (p<0.05) compared to the other twogroups. Protein consumption and digestibility in group with diet A and B was lower (p<0.05)compared to control animals. However, digestibility for dry matter, fat, starch and energy wereabout 90%, meaning that the rations were considered adequate in developing obese monkey.The diet formula containing tallow and egg yolk increased body weight based on BMI criteriaabove 25 kg/m2.Key words: obesity, cynomolgus, egg yolk, body mass index, digestibility
PEMANFATAN EKSTRAK JAMUR TIRAM (PLEUROTUS SPP.) PADA PENYIMPANAN DAGING AYAM PADA SUHU RUANG (26OC) Saskiawan, Iwan; Sukarminah, Een; Lanti, Indira; Marta, Herlina; Nabila, Putri
JURNAL BIOLOGI INDONESIA Vol 13, No 2 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i2.3402

Abstract

ABSTRACTRecently, food preservation especially for meat is becoming one of the subjects of food technology that is still developed. Organic acids is commonly used for food preservative. Meat is easily destroyed mainly by Staphylococcus aureus and Escherichia coli. Oyster Mushroom (Pleurotus spp.), well known as an edible mushrooms is one of the food resources that have antimicrobial activity. The application of oyster mushroom extracts in chicken meat is done using extract concentrations of 0%, 12.5%, 18.75%, 25%, 31.25% and 37.5% where 12,5% is the MIC to inhibit E. coli and S. aureus. The results showed that the application of brown oyster mushroom extracts with concentration of 31.25% can mantain freshness of chicken meat for 12 hours of storage at room temperature (26oC) with the total number of microbes of 8.6 x 105 cfu/g, the total number of E. coli of 0.3 x 101 cfu/g, the total number of S. aureus of 7.7 x 101 cfu/g. It was appropriate with Indonesian National Standard ( SNI 3924:2009). It required the condition such as no decomposition, pH 5.53, color organoleptic value of 3.42 (fair to good) and texture organoleptic value of 3.53 (fair to good).Keywords : oyster mushroom extract, antimicrobial, chicken meat preserving

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