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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia
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INDONESIA
Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 19 Documents
 1   2 
Search results for , issue "Vol 15 No 1 (2014)" : 19 Documents clear
Aktivitas Ekstrak Daun Kelor Terhadap Sel-T Helper dan Sel-T Sitotoksik pada Mencit yang Diinfeksi Salmonella thypi (ACTIVITY OF AQUEOUS LEAF EXTRACT OF HORSERADISH TREE ON HELPER T- CELL AND CYTOTOXIC T- CELL IN MICE INFECTED WITH SALMONELLA THYPI) Akhmad Fathir; Muhaimin Rifa’i; Widodo .
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Typhoid fever is caused by Salmonella typhi infection, and it is a still problem in many developingcountries, including Indonesia. Typhoid fever occurs due to T cells, immune system, especially CD4+ andCD8+ T cells, are deficient. This condition can cause S. thypi infects human body cells . The study aim wasto evaluate profile CD4+ and CD8+ T cells in mice spleen (Mus musculus) infected with  S. thypi afterinducted with aqueous leaf extract of horseradish tree. An experimental laboratory studiy was conductedusing completely factorial randomized design. Mice were divided into two groups, ie non infection group(induced with aqueous leaf extract of horseradish tree, at dose 0 mg/kg BW, 14 mg/kg BW, 42 mg/kg BWand 84 mg/kg BW) and infection group, the Micewere infected with S. thypi (induced with aqueous leafextract of horseradish tree, at dose 0 mg/kg BW, 14 mg/kg BW, 42 mg/kg BW and 84 mg/kg BW). The resultshowed that aqueous leaf extract of horseradish tree increased the number CD4+  of and CD8+ T cells  in allgroups of mice in conclusion administration of aqueous leaf extract of horseradish tree at high dose havecauses immunosuppressive in immune system. Aqueous leaf extract of horseradish tree have aimmunostimulatory and immunosuppressive functions in CD4+ and CD8+ T cells.
Fourier Transform Infrared Sebagai Metode Alternatif Penetapan Tingkat Stres pada Sapi (FOURIER TRANSFORM INFRARED AS AN ALTERNATIVE TOOL FOR DETERMINING OF STRESS IN COW) Pudji Astuti; Claude Mona Airin; Slamet Widiyanto; Amelia Hana; Hera Maheshwari; Luthfiralda Sjahfirdi
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Stress in animal is a condition of nonspesific discomfort which cause of non-specific immune defects,failure of reproduction, and decreased of meat carcass until the death of  animals. To determine stress ofcattle,  it will be invented stress detector using a non-invasive method based on the spectroscopy FourierTransform Infrared (FTIR). Basically,  FTIR will detect component in compound of cathecolamine andcortisol as ketone (= O) and methyl (= CH 3). Furthermore, each group of components will be detected indifferent of absorbant and wavelength.   The results showed that average level of cortisol in female beefcattle durimg resting eriod was 38,48±21,53 ng/dL, on time of slaughtering were 116,88±112,59 ng/dL. Forbull, which were resting  20,42±9,25 ng/dL; when animal was slaughtered level of cortisol was  67,61±41,62ng/dL. Using FTIR, it was showed that compound of metil was absorbed well. Animals with udder stresscondition have been recorded on the wave lenght of 2777-3456 nm.   It has been concluded level of cortisolon cattle which were resting is significantly different from animal which were slaughtering P(< 0.05),where cortisol would increase drastically. Using Calibration of FTIR indicated resting animals only havefewer value of absorbance than animals which slaughtered.  FTIR is a very prospect method for makingstress indicator.
Profil Farmakokinetik Amikasin Pemberian Intravena Melalui Vena Sublingualis dan Coccygea pada Ular Sanca Batik (PHARMACOKINETIC PROFILE OF AMIKACIN ADMINISTERED INTRA VENOUSLY VIA SUBLINGUAL AND COCCYGEA VEINS IN BROGHAMMERUS RETICULATUS) Agustina Dwi Wijayanti; Slamet Rahardjo; Antasiswa Windraningtyas Rosetyadewi; Adi Tri Septyanto
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The research was conducted to find out the pharmacokinetic profile of amikacin in sanca batik snake(Broghammerus reticulatus) which is expected to be beneficial in the therapy management of reptiliaes.The eight of adult snakes with averages body weight of 2-14 kg were used and they were devided into twogroups (n=4). Amikacin (5 mg/kg bw) was given by sublingualis (anterior) or coccygea (posterior) venous ingroups, respectively. Blood samples were collected by intracardiac puncture to all snakes at minutes 1,5,10, 30, 60, 120, 240, 480, 960, 1440 (24 hours) and 2880 (48 hours) post administrations. Bloods werecollected using heparinized tubes and sentrifuged at 2500 G to obtain the plasma. The Plasma sampleswere stored at -200c. Plasma were firstly extracted with trichloroacetid acid solution 10% and then injectedinto High Performace Liquid Chromatography Shimadzu 6.1. The results of amikacin levels werestatistically lower on sublingualis vein administration as compared to that of coccygea vein administrationusing Student T-Test (P<0.05). The pharmacokinetic parameters were calculated with non compartementalmethod resulted for anterior application : Vd 3.6 L, clearance 0.066 mL/minute/kg, AUC 75.384 ug/mL.minute and for posterior application : Vd 0.78 L, T1/2 213.09 hours, clearance 0.427 mL/minute/kg,AUC 117.143,7 ug/mL/minute.
Lumba-Lumba Hidung Botol Laut Jawa Adalah Tursiops aduncus Berdasar Sekuen Gen NADH Dehidrogenase Subunit 6 (VERIFICATION BOTTLENOSE DOLPHINS FROM JAVA SEA IS TURSIOPS ADUNCUS BASED ON GENE SEQUENCES OF NADH DEHYDROGENASE SUBUNIT 6) Rini Widayanti; Yuda Heru Fibrianto; Woro Danur Wendo
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Bottlenose dolphins (Tursiops sp.) is one of the aquatic mammals widely spread in the marines ofIndonesia archipelago, especially the Java Sea. The taxonomy of the genus Tursiops is still  controversial.The purpose of this study was to examine the molecular basis of Tursiops sp of Java sea marine origin onthe basis of its NADH dehydrogenase gene subunit 6 (ND6) sequences. Samples of blood were collectedfrom five male bottle nose dolphins from captivity of PT. Wersut Seguni Indonesia. DNA was isolated,amplified by polymerase chain reaction (PCR), sequenced, and analyzed the data using the MEGA v. 5.1program. The results of PCR amplification was 868 base pairs (bp), DNA sequencing showed that 528nucleotides were ND6 gene, nucleotide at the position of 387 could be used to distinguish the bottle nosedolphins Java marine origin with T. aduncus.   Filogram using Neighbor joining method based on thenucleotide sequence of the gene ND6, showed that bottle nose dolphins Java marine origin belong to groupof T. aduncus.
Keutuhan Membran Spermatozoa Disekuensing Sentrifugasi Gradien Densitas Percoll Berpengencer Andromed dan CEP-2 yang Ditambahkan Kuning Telur (MEMBRANE INTACT OF SPERMATOZOA FOLLOWING SEXING USING PERCOLL DENSITY GRADIENT CENTRIFUGATION IN ANDROMED AND C Yudha Fika Diliyana; Trinil Susilawati; Sri Rahayu
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to observe the best extender in protecting the membrane of bovine spermatozoafollowing sexing by percoll density gradient centrifugation. Freshly collected semen were obtained fromBalai Besar Inseminasi Buatan Singosari-Malang. The semen were diluted in andromed and CaudalEpididymal Plasma-2 (CEP-2) added with 10% egg yolk extenders.The sperm membrane integrity wasobserved using Hypo-osmotic Swelling Test (HOST). Sperm capacitation and acrososome reaction wereassessed using Chlortetracycline Fluorescence Assay.The results showed that andromed and CEP-2 addedwith 10% egg yolk were able to retain the sperm membrane integrity, whereas sperm capacitation andacrosome reaction were kept low. Caudal Epididymal Plasma-2 (CEP-2) added with 10% egg yolk seemedto give better protection towards the sperm membrane intact in comparison to andromed extender.
Kerapuhan Sel Darah Merah Sapi Bali (THE FRAGILITY OF ERYTHROCYTES OF BALI CATTLE) Siswanto .; I Nyoman Sulabda; I Gede Soma
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

An experimental study was conducted in order to evaluate the   of erythrocyte on bali cattle atVeterinary Physiology Laboratory, Faculty of Veterinary Medicine, Udayana University, Denpasar, duringone month.  Fifty blood samples were taken from bali cattle which slaughtered at Pesanggaran abattoir,Denpasar. The erythrocyte fragility was determined by using method Red Cell Fragility Procedure (2001).Results showed that the fragility (initial haemolysis) on red blood cell of bali cattle was observed, atranged between 0.45% - 0.55% NaCl , while for total  haemolysis range between  0.30 - 0,35 % NaCl.
Faktor-Faktor Risiko Mastitis Subklinis pada Kambing Peranakan Etawah di Kabupaten Sleman, Yogyakarta (RISK FACTORS OF SUBCLINICAL MASTITIS ON ETTAWA CROSSBRED GOAT IN SLEMAN REGENCY, YOGYAKARTA) Widodo Suwito; Widagdo Sri Nugroho; Bambang Sumiarto; Agnesia Endang Tri Hastuti Wahyuni
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

In Sleman, a regency in Yogyakarta special region, Etawah crossbred goats are excessively bred for thedairy produce called the goat’s milk. Subclinical mastitis is one of diseases which reduce the yield of goat’smilk. The aim of this study was to determine the risk factors that contribute to the subclinical mastitis onthe Etawah crossbred goats in Sleman. The 200 samples one of which contains 10 mL of goat’s milk weretaken from the udders of the Etawah crossbred goats from the eight goat farms in Sleman. The 200samples were analyzed for the subclinical mastitis using California Mastitis Test (CMT). The data of riskfactors were gathered through a questionnaire. The risk factors on Etawah crossbred goats in Sleman weredetermine with the use of bivariate analysis chi square (X)2, odds ratio (OR) and relative risk (RR). Thegoat’s milk which subclinical mastitis was isolation and identification of bacteria based on biochemicaltests. The risk factors that cause the subclinicall mastitis on Etawah crossbred goats in Sleman were (1)milk yield (X2=14.23; OR=6.52; RR=4.42), (2) age status of lactation (X2=1.60; OR=59.09; RR=17.94), (3)age of weaning (X2=26.06; OR=2.22; RR=1.91), and (4) Body Condition Score (BCS) (X2=13.89; OR=1.29;RR=1.22). Goat’s milk which subclinicall mastitis were isolated  Bacillus sp  (70%), Staphylococcus sp(33%), Pseudomonas sp (29%), Streptococcus sp (25%), Corynebacterium sp (12%), and E. coli (4%).
Residu Deltamethrin Terlacak pada Hati Ayam Pedaging yang Dipasarkan di Yogyakarta (DETECTION OF DELTAMETHRIN RESIDUE IN BROILER LIVER TRADED IN YOGYAKARTA) Gagak Donny Satria; Agustina Dwi Wijayanti; Puspa Wikan Sari; Dwi Cahyo Budi Setiawan; Nisa Hakimah; Annisa Rahmawati
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The consumer assurance to get safe, hygienic, whole, and halal food is an important concern. Analysisof chemical residue is one of some ways  to examine food safety. The objective of this research was to detectdeltamethrin residue in broiler liver sold in Yogyakarta, Indonesia. The isocratic HPLC method was usedin this research, using Shimadzu 6.1, 80% of Acetonitrile in Aquabides as mobile phase, with 1ml/minutesof flow rate. C-18 was used as column and the wavelength of UV-Vis detector was 270 nm. The result of theresearch was get from HPLC’s analyses. Deltamethrin’s peak area would be presented in retention time 8-12minutes with specific profile of curve. The result of the research showed that deltamethrin was positivelydetected in 13 broiler livers. It’s concluded that broiler livers that sold in Yogyakarta are detected to containdeltamethrin-contaminated.
Jumlah dan Ukuran Nodul Tumor Kolorektal Berbanding Lurus dengan Ekspresi Siklooksigenase-2 (THE CORRELATION OF THE NUMBER AND THE SIZE OF COLORECTAL TUMOR NODULE WITH CYCLOOXYGENASE-2 EXPRESSION) Risfah Yulianty; Riska Nufika; Sitarina Widyarini
Jurnal Veteriner Vol 15 No 1 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Study chemically induced colon cancer with 1,2-Dimethylhydrazine (DMH) has been widely used.DMH induction to rodentia as animal model have been similarity with colon cancer morphlogy of human.Cyclooxygenase-2 (COX-2) are important enzymes that affect carcinogenesis.COX-2can be induced byvarious stimuli, including inûammation, growth factors, and cytokines produced by tumor cells. The aim ofthis study is  to investigate the correlation between the expression  of COX-2 and the doses variation ofDMH, the number and the size of colorectal tumor nodule.Six male Wistar rats were randomly divided into 4 groups each group consists of six rats.  Group I is control group.  Group II, III, and IV were given 20, 40,and 60 mg/kg BW of DMH respectively.  DMH  was subcutaneously injected in the hip area, once a week for15 weeks.  All animals were sacrificed at week 26th, the colon were removed and fixed in 10% bufferformalin for macroscopic and microscopic examination.  The results of this study showed that there is acorrelation between various doses of DMH  (P<0.05) with number of tumor nodule but not with the size ofcolorectal tumor nodule (P>0.05). Microscopic examination by using HE staining shows that inductionwith doses variation ofDMHresultin different morphololical form of tumour tissue.  Induction of 20, 40,and 60 mg/kg BW DMH result in adenoma(100.00%),adenoma and adenocarcinoma (33.30% and 66.67%),and 100.00% adenocarcinoma respectively.  The happens of COX-2 also correlate with in this studythatexpression of COX-2 correlate with the number and size of colorectal tumor nodule (P<0.05). However,there is no correlation between COX-2 expressionand doses variation of DMH.  In conclusion, there is adose-dependent response in the number and the size of colorectal tumor nodule.

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