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I G. Made Krisna Erawan
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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
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Articles 20 Documents
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Search results for , issue "Vol 16 No 4 (2015)" : 20 Documents clear
Pelacakan Gen Env-TM Virus Penyakit Jembrana Galur Tabanan 1995 dengan Metode Nucleic Acid Sequence Based Amplificaton Asmarani Kusumawati; Atik Ratnawati; Ida Arlita Wulandari; Sri Hartati; Tri Untari
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Jembrana disease is an infectious disease in Bali cattle cause by a member of lentivirus calledjembrana disease virus (JDV). It causes an acute and severe disease syndrome with short incubationperiod. As the disease has spread to several areas in Indonesia, a simple and rapid detection method isrequired. The objective of this study to apply rapid diagnostic method for JDVTabanan 1995 strain basedon Nucleic Acid Sequence Based Amplification (NASBA) methods targeting env-tm gene. The steps of thisresearch consisted of viral RNA isolation from organ and blood of cattle experimentaly infected withJDVTabanan 1995 strain . RNA amplification was conducted by NASBA using waterbath. The NASBAproducts were then separated on 2 % agarose gel. Using this technique JDV positive result was obtainedfrom organ samples such as spleen, liver, lung, prefemoralis lymph node, prescapularis lymph node andblood generating a RNA fragment of 207 bp. In this study, diagnosis method for env tm of JDV Tabanan1995 strain can be conducted by isothermal amplification NASBA.
Diagnosis Infeksi Streptococcus suis serotipe-2 pada Babi Secara Serologi dengan Muramidase Released Protein (SEROLOGICALLY DIAGNOSE OF STREPTOCOCCUS SUIS SEROTYPE-2 INFECTION IN PIGS BASED ON MURAMIDASE RELEASED PROTEIN) Siti Isrina Oktavia Salasia; Clara Ajeng Artdita; Mitra Slipranata; Sidna Artanto
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Streptococcus suis is a bacterial pathogen causing disease of pigs that characterized by meningitis,bronchopneumonia, arthritis, pericarditis, polyserositis and septicaemia. S. suis especially serotype 2 caninfect human (zoonotic) with a special symptom of meningitis. The aim of this research was to detect S.suis infection based on muramidase released protein (MRP), as an important virulence marker of S. suis.S. suis serotype 2 strain P171 with phenotype of MRP+EF+ was used in this research. The MRP antigen wasextracted using lysozyme and separated by using sodium dodecyl sulphate-polyacrylamide gel electrophoresis(SDS-PAGE). Balb/c mice were imunized with 136 kDa MRP to produce antibody against MRP. Theantibody was evaluated by using enzyme linkage immunosorbent assay (ELISA). The results of the researchshowed that the antibody against MRP with molecular weight of 136 kDa could be produced on Balb/Cmice with the highest absorbance of 3,889 and could be used to detect field sera from infected pigs with200x dilution using ELISA antigen capture. Antibody against MRP could detect serologically of S. suisinfection in pigs in Papua with 50% seropositivy by using ELISA antigen capture and 40% by using dot blot.
Antigen Ekskretori-Sekretori Cacing Jantung (Dirofilaria immitis) Jantan dan Betina yang Berpotensi Sebagai Marka Diagnosis (EXCRETORY-SECRETORY ANTIGENS OF MALE AND FEMALE HEART WORMS (DIROFILARIA IMMITIS) WHICH POTENTIALLY AS A DIAGNOSTIC MARKER) I Gusti Made Krisna Erawan; Ida Tjahajati; Wisnu Nurcahyo; Widya Asmara
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Heart worm (Dirofilaria immitis) is the causative agent of a serious parasitic disease in dogs.Dirofilariasis is generally diagnosed by microfilariae examination and specific antigen testing. Microfilariaeexamination has low sensitivity due to occult infections. The available antigen test at this time is able todetect circulating antigens secreted by adult female worms only. The aim of the present study was toidentify male (MES) and female (FES) heart worms excretory-secretory antigens which have the potentialas a diagnostic targets. Identification of antigen was done by sodium dodecyl sulphate polyacrylamide gelelectrophoresis (SDS-PAGE) and Western Blotting analysis. The results of this study indicated that therewere differences between the MES and the FES profiles. The results showed 12 bands in MES (14–118kDa) and 18 bands in FES (10–205 kDa). Protein with a molecular weight of 59 kDa has the potential asdiagnostic markers of dirofilariasis.
Aktivasi dan Tingkat Perkembangan Embrio Partenogenetik Mencit Setelah Dipapar Calcimycin dan Ionomicyn (ACTIVATION AND DEVELOPMENT RATE OF MICE PARTHENOGENETIC EMBRYOS EXPOSURED IN CALCIMYCIN AND IONOMICYN) Wilmientje Marlene Nalley; Thomas Mata Hine
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of study was to find out the best concentration and exposure time of calcimycin andionomycin in order to produce parthenogenetic embryos. Female Swiss Webster mice were fisrtlyprimed with Pregnant Mare’s Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin (hCG)with an interval of 48 hours. Sixteen hours after injection of hCG oocyte was collected by Dulbecco’sPhosphate Buffer Saline (dPBS) as a flushing medium. To separate the eggs from cumulus cells wereused hyaluronidase enzyme. The good quality oocytes were incubated in activation medium that isionomycin or calcimycin with a concentration of 3, 6, or 9 ìM and exposure time 1, 4, or 7 minutes. Toyield diploid embryos were used 5 ?g/ml cytochlasin B for four hours at 37°C, 5% CO2. Activatedoocytes characterized by the formation of pronuclei washed three times in Potassium SimplexOptimization Medium (KSOM) and subsequently cultured in the same medium until blastocyst stage.The results showed that oocytes activated at calcimycin, the best results was presented at concentration6 ?M and exposure time four minutes, i.e. activation rate reached 96%, cleavage rate 82% and blastocystrate 28%. On the other hand, oocytes activated in ionomycin, the best results was presented atconcentration 3 ?M and exposure time four minutes, i.e. activation rate reached 82%, cleavage rate64% and blastocyst rate of 4%. It was concluded that the best concentration and exposure timecalcimycin on mice oocytes were 6 ?M for four minutes, whereas ionomycin were 3 ?M for four minutes.
Pengembangan Media Padat untuk Menumbuhkan Mycobacterium bovis (DEVELOPMENT OF SOLID MEDIUM FOR MYCOBACTERIUM BOVIS CULTIVATION) Mazdani Ulfah Daulay; Mirnawati Sudarwanto; Widagdo Sri Nugroho; Etih Sudarnika
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Mycobacterial culture provides definitive diagnosis of tuberculosis (TB), but commercially readyto-use culture media for Mycobacterium bovis are rarely available. The aims of this study were todevelop and to evaluate the ability of M. Bovis to grow in Modified Ogawa Agar (MOA) in comparisonwith the available culture media, such as Löwenstein Jensen (LJ) and Modified Ogawa (MO). Eachmedia were inculation with 0.1 ml suspension of 105 CFU/mL M. bovis and M. phlei in PhosphateBuffer Saline (PBS) and each media was replicated in five tubes. Mycobacterium phlei grew in everymedium since day 4. M. bovis grew in media LJ and MO since day 17, but failed to grow in mediumMOA. The recovery rate of M. phlei in LJ and MOA were significantly different. The ability of MOA tocultivate M. phlei was different from LJ. Colonies of M. phlei in MOA were easier to be harvested, muchsimpler to prepare, and more feasible than medium LJ. The recovery rate of M. bovis in media LJ andMO were not significantly different, but medium MO were much simpler to prepare and more feasiblethan medium LJ. Media MOA were able to cultivate M. phlei, but proven unable to cultivate M. bovisin this research.
Deteksi Bovine Herpesvirus-1 Secara Immunohistokimia pada Membran Korioallantois Telur Ayam Berembrio (IMMUNOHISTOCHEMISTRY DETECTION OF BOVINE HERPESVIRUS-1 IN CORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYONATED EGG) Yuli Purwandari Kristianingrum; Charles Rangga Tabbu; Bambang Sutrisno; Sitarina Widyarini; Kurniasih .; Tri Untari; Asmarani Kusumawati
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Infectious Bovine Rhinotracheitis (IBR) is caused by Bovine Herpes virus-1 in the cattle. The clinicalsigns demonstrate depression, anorexia, swelling of the vulva, redness of the vestibule, pustule and ulceron the vaginal mucosal. Based on previous research, IBR virus from the nasal swab could be grown inchorio-allantoic membrane of embryonated chicken eggs. This study aim was to confirm whether IBR virusin cattle could be grown in embryonated chicken eggs as a substitute for cell culture. A total of five nasalswab samples from the cows that were positive for IBR infection (diagnosed by Polymerase Chain Reactionand cell culture) were inoculated on the chorio-allantois membrane of embryonated chicken eggs.Observation of lesions performed at 3-5 days after inoculation. Re-inoculation (passage) was done threetimes. Pock characteristic lesions were observed on the corioallantoic membrane with the size of 5-7 mm,rounded shape, opaque edge, with necrosis in the central area. Furthermore, pock lesions were processedfor hematoxylin and eosin staining and immuno-histochemistry. The result of hematoxylin and eosinstaining showed that the formation of intranuclear inclusion bodies and vacuolization of the epithelial cellof membrane was observed. Immuno-histochemistry staining showed positive reaction for antibodiesagainst BHV-1 in the epithelial cells membrane. In conclusion, embryonated chicken eggs could be usedas a medium for detection of IBR.
Perbaikan Fenotipe Pertumbuhan Anak Babi Lokal Melalui Penyuntikan Gonadotropin Sebelum Induk Dikawinkan (IMPROVEMENT OF GROWTH PHENOTYPE OF LOCAL PIGLET BY GONADOTROPHIN INJECTION OF SOW PRIOR TO MATING) 1Debby Jacqueline Jochebed Rayer; Muladno .; Hera Maheshwari; Wasmen Manalu
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

An experiment was designed to study the growth phenotypes of piglets born to sows injected withpregnant mare serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG) prior to mating inorder to improve endogenous secretions of pregnant hormones during pregnancy. The experimental sowsused in this study were 10 local breed sows with body weight ranges of 30-40 kg. Before mating, estrouscycles of the experimental sows were synchronized by injecting 3.75 mg prostaglandin twice with 14 daysinterval. The experimental sows were then divided into two groups, each consisted of 5 sows. The firstgroup was injected with 200 IU PMSG and 100 IU hCG per sow at the same time with the secondprostaglandin injection (day 15th), while the second group was not injected with PMSG and hCG but it wasinjected with NaCl 0.95% as a control. After showing estrous behavior, the experimental sows were mixedwith selected boars for natural mating. The pregnant sows were maintained until farrowing and weaning. Variable measured were body weights and body lengths and leg heights of the piglets at birth andweaning. The results showed that injection of the sows with PMSG and hCG prior to the mating, increasedbirth weight by 76.92% and total birth weight of live piglets per sow by 265.6% as compared to control.Piglets born to sows injected with PMSG and hCG prior to mating had higher survival rate with adramatically decreased mortality and a higher pre-weaning growth rate that finally increased total weightof weaned pigs per sows dramatically by 107.44% (increased 2 times) as compared to control. It is concludedthat the growth phenotypes of local piglets could be improved by injecting the sows with gonadotropinbefore mating.
Antibiotic Sensitivity Pattern of Staphylococcus aureus and Escherichia coli Isolated from Bovine Fresh Milk (POLA SENSITIVITAS ANTIBIOTIK TERHADAP STAPHYLOCOCCUS AUREUS DAN ESCHERICHIA COLI YANG DIISOLASI DARI SUSU SAPI SEGAR) Lucia Ratna Winata Muslimin; Firzan Nainu; Rochmat Himawan
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aims of this study were to determine the sensitivity of S.aureus and E. coli isolated fromfresh milk against against several antibiotics and to determine the safety of the milk for humancomsumsion. Milk was collected from milking diary cow and was used for the bacterial isolation. E.coli were were identified using Total Plate Count (TPC), Gram staining, their growth on Endo Agarand Eosin MethyleneBlue Agar, Biochemical analysis including glucose, lactose, sucrose,maltose, andsorbitol would be followed by Sorbitol Mac Conkey Agar Test for the identification of E.coliO157:H7.The isolation and identification of S.aureus were performed using Gram stain, TPC, growth on BairdParker Agar and Mannitol Salt Agar. S. aureus and S. epidermidis were differentiated by coagulaseand catalase tests. The antibiotic sensitivity tests for both S. aureus and E.coli were carried out usingthe following antibiotics: ampicillin, bacitracin, vancomycin, cefoperazone, ceftriaxone, cefotamine,cefuroxime, cefepime, cefazoline, ceftazidime, chloramphenicol, tetracycline, doxycycline, amikacin,kanamycin, neomycin, ertapenem, meropenem, imipenem, erythromycin, gentamycin, nalidixic acid,ciprofloxacin, levofloxacine, norfloxacine, ofloxacin, and novobiocin. Fresh milk obtained from thefarm was positive for S.aureus and E.coli and resistant to most antibiotics tested. The best antibioticsfor S. aureus were imipenem (54.1 mm), ampicillin (42.3 mm), cefazolin (41.6 mm), doxycycline (41.15mm), and for E.coli were Imipenem (30.1 mm), ertapenem (29.5 mm), and meropenem (25.35 mm). Thebovine fresh milk examined was contaminated by S.aureus and E.coli and to some extent, were alsoresistant to most antibiotics tested.
Residu Zeranol dalam Daging Sapi yang Diimpor dari Australia dan Selandia Baru Melalui Pelabuhan Tanjung Priok (ZERANOL RESIDUE IN BEEF MEAT IMPORTED FROM AUSTRALIA AND NEW ZEALAND THROUGH THE PORT OF TANJUNG PRIOK) Siti Khadijah; Hadri Latif; Agatha Winny Sanjaya
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Zeranol is one of the synthetic growth hormone produced from mycotoxin that could affect humanhealth. The objective of this study was to determine zeranol residue in beef meat imported from Australiaand New Zealand using Enzyme-Linked Immunosorbent Assay (ELISA). The results showed that zeranolresidue was detected in 5 of 59 meat samples (8,5 %) of Australia and 1 of 59 meat samples (1,7 %) of NewZealand, with mean concentration of 0,644±0,157 ppb and 0.680±0.00 ppb, respectively. There were nosignificant differences in the concentration of zeranol residue between the meat from both countries(p>0,005). In addition the concentration of zeranol residue was below the National Standardization Agencyof Indonesia Maximum Residue Limit (MRL) which is 2 ppb.
Aktivitas Antibakteri Ekstrak Etanol dan Destilat Jahe dan Sirih terhadap Mycoplasma gallisepticum dan Escherichia coli (ANTIBACTERIAL ACTIVITY OF ETHANOL EXTRACT AND DESTILAT OF GINGERS AND PIPER BETLES AGAINST MYCOPLASMA GALLISEPTICUM AND ESCHERICHIA CO Min Rahminiwati; Aulia Andi Mustika; Agung Zaim; Lina Novianti Sutardi
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Betle and ginger juice extracts have been reported to have antibacterial activity against E. coliand M. gallisepticum. However, fractionation analysis showed the have differences in their antibacterialpotency which appeared to be associated with the nature of the solvent polarity. This study wasconducted to obtain information about antibacterial activity of Piper betles and gingers extract againstE. coli and M. gallisepticum. Three types of betle, P. betle, P. betle var nigra, P. crocatum, wereexamined for their antibacterial activity using disc method and three types of ginger, Zingiber officinale,Z. officinale Linn var rubrum, and Z. majus rumph which were extracted by soxhletation using ethanol30%, 60% and 90% as well as distestillation for three and five hours. Piper betle and P. crocatumconsistently have antibacterial effect against E. coli whereas Z. officinale consistently has antibacterialeffect against M. gallisepticum, either extracted by distillation or soxhletation. Piper betle is potentialyield of distillate and extract that has the highest antibacterial activity against E. coli, and M.gallisepticum with inhibitory zone were 9.76 mm and 22 mm respectively.

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