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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Uji Aktivitas Pertumbuhan Enterobacter cloacae Selulolitik Aerob Rumen-1 Isolat Asal Limbah Cairan Rumen Sapi Peranakan Ongole (GROWTH ACTIVITY ASSAY OF CELLULOLYTIC BACTERIA ENTEROBACTER CLOACAE SAR 1 (CELLULOLYTIC AEROB RUMEN 1) ISOLATED FROM ONGOLE CRO Tri Nurhajati; Koesnoto Soepranianondo; Widya Paramita Lokapirnasari
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (103.273 KB)

Abstract

This study aimedto know the growth activityofcellulolytic bacteria EnterobactercloacaeSAR 1isolatedfrombovinerumen fluidwaste. Isolates that had been cultured were taken as much as 10 mL and thentransferred to100 mL growth medium in Erlenmeyer flask. Culturesuspensions were incubated in a shakerincubator (37°C, 120 rpm). Optical density was measured at ë 600 nm by taking as much as 1 mLsampling with interval of two hours for 24 hours (hour 0; 2; 4; 6; 10; 12; 14; 16; 18; 20; 22; 24). The firstsampling was done at 0thhour and continued until OD values †showed a clear decline. Optical density wasmeasured with a UV-Vis spectrophotometer at wave length ë 600 nm. Growth curve was obtained from theresult of absorbance measurement on the time. Optimum growth production of E.cloacae SAR 1 occurredat the 12thhoursof incubation, optimum temperature of 35°C and optimum pH 6.
Pemberian Gamal Tambahan dalam Ransum Meningkatkan Neraca Nitrogen dan Populasi Mikrob Proteolitik Rumen Sapi Bali (ENHANCEMENT PROVISION OF GLIRICIDIA SEPIUM IN DIET INCREASE NITROGEN BALANCE AND POPULATION OF RUMEN PROTEOLITIK MICROORGANISM OF BALI CATT Ni Nyoman Suryani; I Gede Mahardika; Sentana Putra; Nengah Sujaya
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.635 KB)

Abstract

This research aimed to study the effect of different forage composition in diet on nitrogen balance andmicrobial population of Bali cattle. Randomized Block Design consisted of four feed treatments with 3block of weight live as replicates were used in this study. Body weight of male bali cattle used rangedbetween 181-265 kg. These four treatments based on dry matter were: A (45% elephant grass + 0% ricestraw + 15% glyricidia + 10% calliandra + 30% concentrate); B (30% elephant grass +10% rice straw + 20%glyricidia + 10% calliandra+ 30% concentrate) ; C (15% elephant grass +20% rice straw + 25% glyricidia+10% calliandra + 30% concentrate) and D (0%elephant grass + 30% rice straw + 30% glyricidia + 10%calliandra+ 30% concentrate) . Variables measured were nitrogen balance and rumen microbial population.The collected data were analyzed by analysis of variance. The result showed that nitrogen intake in cattlefed with diet C was significantly (P<0.05) higher than these in other treatments and nitrogen retention(P<0.05) was significantly higher as compared to those fed with diet A. Amylolytic and cellulolytic bacterialpopulations were not significantly different (P>0.05) among all treatments, but the population of proteolyticbacteria was found the lowest (P<0.05) in cattle fed with diet A. It can be concluded that increasedglyricidia and rice straw in the diet could increased nitrogen intake, nitrogen retention and proteolyticbacterial population.
Detoksifikasi Mikotoksin Melalui Optimalisasi Fungsi Rumen dengan Pemberian Ragi (MYCOTOXIN DETOXIFICATION THROUGH OPTIMIZATION THE RUMEN FUNCTION BY YEAST) Dadik Pantaya; Komang Gede Wiryawan; Dwierra Evvyernie Amirroenas; Suryahadi .
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (198.305 KB)

Abstract

Mycotoxins are toxic metabolites produced by some fungal species commonly found in food and feed,particularly in cereals. In intensive production systems, dairy cattle are commonly fed with cereal-richdiets and, consequently, are more exposed to micotoxins. Besides, such diet is often associated with ahigher risk of rumen acidosis which can also affect the performance and the helath of animal. In addition,the efficacy of microbial detoxification can be reduced during acidosis. For instance, some authors observeda decrease in the number of protozoa that are responsible for the degradation of some mycotoxins. Anotherconsequence of acidosis is the potential modification of ruminal absorption of mycotoxins, which until nowhas received scarce attention. Yeast Saccharomyces cerevisiae, probiotic additives have been shown toreduce the post-feeding drop in rumen pH and to increase the number of ruminal protozoa. This effect canbe positive in reducing the absorption and toxicity of mycotoxins in ruminantia.
Pertumbuhan dan Keseragaman Warna Bulu Ayam Persilangan Balik (BC2) Hasil Seleksi Genetik Persilangan Ayam Pelung dengan Ayam Pedaging (GROWTH AND PLUMAGE COLOR UNIFORMITY OF BACK CROSS (BC2)CHICKEN RESULTED FROM GENETICS SELECTION OF PELUNG CHICKEN Ayudha Bahana I. Perdamaian; Trijoko Trijoko; Budi Setiadi Daryono
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.397 KB) | DOI: 10.19087/jveteriner.2017.18.4.557

Abstract

Research aim to derive an excellent hybrid to be positioned as meat-type chicken. An excellent breed which possess uniform morphological character was archived by genetic selection through back-crossed mating shceme in focus on growth rate and plumage color uniformity. Day Old Chicken (DOC) resulted from parent stock broiler cobb 500 and Pelung chicken originated from Cianjur district, West java mating were intensively reared for seven week from hatch. Each chicken weighted every seven day and morphological character assessed at seven weeks old. Observe variable are heterocyst, coefficient Inbreeding (Fx), Inbreeding rate (F), plumage and shank characteristic proportion, and its frequency gene alteration through selection. After serial genetic selection, the BC2 chicken has 1129 g body weight and uniform morphological character. Overall body weight of BC3 chicken offspring from BC2 and F1 was deteriorated compared its broiler predecessor because of Inbreeding depression (Fx: 0.4375; F: 0.3125) and heterocyst decrement (H: -39.33) however, morphological appearance were highly resemble pelung chicken. Based on these finding, BC2 chicken was promised meat-type hybrid chicken which has fast growth rate and similar morphological character.
KUALITAS SEMEN SEGAR SAPI BALI PADA DUA KELOMPOK UMUR DAN PENENTUAN KONSENTRASI KRIOPROTEKTAN DALAM PENGENCER TRIS KUNING TELUR (Fresh Semen Quality Of Bali Bull In Two Group Of Age And Determination Of Cryoprotective Agent Concentration In Tris Eggyolk Extender) Anna Nabilla; Raden Iis Arifiantini; Bambang Purwantara
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (142.162 KB) | DOI: 10.19087/jveteriner.2018.19.2.242

Abstract

The aim of this study was to compare the quality of Bali bull fresh semen in productive group (below 10 years old) with over productive group and to evaluate the frozen semen quality of bali bull after cryopreservation in Tris egg yolk extender using Dimethyl formamide (DMF) and glycerol in three different concentrations (5%, 6%, and 7%). Semen was collected using artificial vagina. Semen then evaluated macro and microscopically. Macroscopic evaluation conducted visually and microscopic evaluation with the aid of Computer Assisted Sperm Analysis CASA. Immediatelly after evaluation, semen were divided into six aliquots and diluted with Tris egg yolk (TEY) glycerol 5% (TEYG5), TEY-glycerol 6% (TEYG6), TEY glycerol 7% (TEYG7), TEY-DMF 5% (TKTD5), TEY-DMF 6% (TEYD6) and TEY-DMF 7% (TKTD7). Results of experiment showed that there was no difference (P>0.05) among the all parameters, except for semen volume. Semen volume of productive group was higher than over productive age group. No difference was found in post thawing motility of the spermatozoa in TEY extender with 5% glycerol, and TEY with 5%, 6% and 7% DMF. The higher recovery rate of bali bull spermatozoa demonstrated by spermatozoa in TEY extender with glycerol 5% and DMF 6% (63.33±2.40 dan 68.67±2.33). It was concluded that bali bull semen quality did not differ between productive and over productive ages and post thawing semen quality of bali bull in TEY with 5-7% DMF or 5% glycerol was not also different.
Tampilan Produksi dan Efek Imunomodulasi Ayam Broiler yang Diberi Ransum Berbasis Wheat Pollard Terolah (PRODUCTION PERFORMANCE AND IMMUNOMODULATION EFFECTS ON BROILER GIVEN A PROCESSED WHEAT POLLARD BASED DIET) Bambang Sulistiyanto; Sri Kismiati; Cahya Setya Utama
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (81.759 KB) | DOI: 10.19087/jveteriner.2019.20.3.352

Abstract

An immunomodulator is a natural body defence mechanism due to the stimulation of objects/ environments, both specific and non-specific by means of cellular and humoraldefence. The objective of the study was to prove the immunomodulation ability of broiler chickens up to age 35 days that dietary fed various wheat pollard based rations. The design used in the study was a complete randomized design with six treatments and three replications. The treatment of broiler rats consisted of BR-IAJ (T0), control feed with wheat pollard base (T1), control feed with wheat pollard base plus probiotic (T2), wheat pollard based feed steamed (T3), fermented wheat pollard based feed 40% (T4 ) and fermented wheat pollard based feed 60% (T5). The rations used contained 20,5-22,5% protein with 2900-3100 Kcal metabolic energy. Lohman MB 202 Platinum from PT. Japfa Comfeed used in research with average day old chick (DOC) weight of 36,39 + 2,45 g. Parameters observed included body weight, feed intake, feed conversion ratio (FCR), relative weights of liver organ, thymus, lymph and bursa fabricius. The results showed that wheat pollard-based rations had a significant effect (p <0,05) on the relative weight of liver organ, body weight, FCR and ration consumption, but did not affect the relative weights of spleen organ, thymus and bursa fabricius. The mean relative weight of consecutive liver as follows: 2,47 g (T0), 2,83 g (T1), 3,40 g (T2), 2,99 g (T3), 3,15 g (T4) and 3,06 g (T5). The average body weight, consumption and FCR rations were as follows: 1415,24 g; 136,45 g/day; 2,03 (T0), 775,04 g; 125,19 g/day; 3,40 (T1), 813,17 g; 129,86 g / day; 3,36 (T2), 792,89 g; 128,14 g /day; 3,54 (T3), 892,91 g; 138,33 g /day; 3,27 (T4), 969,56 g; 155,52 g/day; 3,37 (T5). The conclusion of the study was that the ration with the addition of wheat pollard 60% was able to provide an increase in body weight gain and the best immunomodulation seen from the aspect of the proportion of immunomodulating organs to the growth of broiler chickens.
Impacts of Pig Management and Husbandry Farmers Towards Classical Swine Fever Transmission in West Timor Indonesia (DAMPAK MANAJEMEN DAN CARA BETERNAK BABI TERHADAP PENULARAN PENYAKIT CHOLERA BABI DI TIMOR BARAT) Petrus Malo Bulu; Ian Robertson; Maria Geong
Jurnal Veteriner Vol 16 No 1 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Classical swine fever (CSF) is a serious and highly infectious viral disease of domestic pigs and wildboar, which is caused by a single stranded RNA pestivirus. A cross sectional study was carried out onsmall-holder pig farmers in West Timor, in the province of East Nusa Tenggara, Indonesia. The objectiveof this study was to describe the management, husbandry and trading practices adopted by pig farmers inWest Timor. A questionnaire survey was administered to the owners of these pigs (n = 240) to gatherinformation from farmers in order to understand management and husbandry practices in the region. Theresults of the questionnaire highlighted the lack of implementation of biosecurity measures by smallholderfarms in West Timor, which has the potential to increase the risk of their pigs to CSF, as well as toother diseases.
Penentuan Waktu Optimal Kawin Berdasarkan Ultrasonografi Ovarium dengan Gejala Klinis Estrus pada Kambing Peranakan Etawa (DETERMINATION OF OPTIMAL MATING TIME BASED ON OVARY ULTRASONOGRAPHY WITH ESTROUS CLINICAL SYMPTOMS IN ETAWA CROSSBREED GOAT) Yudi Eka Satria; Tuty Laswardi Yusuf; Amrozi .
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to determine the optimal mating time in Etawa Crossbred Goat. This studyused 10 ewes, 2-3 years old, with normal estrous cycle. Initiation of estrous using Controlled Internal DrugRelease Implants (CIDR) intra vaginal and released after 12 days. The development of follicles in theovaries wasobserved using transrectal Ultrasonography (USG) every six hours until reach for 66 hoursafter CIDR removal from the vagina. Clinical signs of standing heat and vulva signs were also observedwhile under USG observation. The follicles were divided into three groups based on their diameter:small(<3 mm), medium (4-4.9 mm) and large (>5 mm) which were observed during theestrous phase. Ovulationwas predicted when large follicles (dominant follicles) havenot been seen anymore. The resulta showedthat the average follicles number in ovary after 36 hours CIDR removal were 0-1 (0.8±0.4 mm) small, 0-1(0.9±0.6 mm) medium and 1-2 (1.7±0.8 mm) large, respectively. The large follicles (7-8 mm) wereseen after36 hours CIDR removal or 24 hours after standing heat. Forty two hours after CIDR removal, the largefollicles werenot being seen anymore. It means, the ovulation were predicted in 36-42 hours after CIDRremoval or 24-30 hours after standing heat. The relationship between follicular development and clinical vulva signs (redness, swelling, mucus) revealed that the highest vulva signs (+++) wereseen in 36-42 hoursafter CIDR removal. It was concluded that the ovulation time occurred in 36-42 hours after CIDR removal,with the high intensity of vulva changes. Therefore the optimal mating time in PE 24-36 hours of standingheat or 36-42 hours after CIDR removal.
Cemaran Kapang pada Pakan Sapi dan Uji In Vitro Sirih terhadap Pertumbuhan Kapang Aspergillus flavus (MOLD CONTAMINATION IN CATTLE FEED AND IN VITRO ASSAY OF PIPER BETEL AGAINTS GROWTH OF MOLD CONTAMINANT ASPERGILLUS FLAVUS ) Riza Zainuddin Ahmad; Djaenudin Gholib
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (101.083 KB) | DOI: 10.19087/jveteriner.2017.18.3.453

Abstract

Contamination of mold in feed and Ingridients of feed is important because pathogenic and toxigenic mold will contaminate and cause mycotic and mycotoxicosis on livestock especially cattle. Information regarding the data is required in an attempt to controll of mold contaminant. Base on the previous study piper betel leaf (Piper betle) showed high activity as antimold. The aim of this study were to obtain data of mold contamination in cattle feed and ingredients of feed from the provinces of Banten, Lampung, Jakarta and West Java, and to test piper betel as an antimold herbal from traditional medicinal plants originated from Indonesia. Isolation and identification of fungi were conducted on the flour, glycerides, onggok, corn, peanut, coconut, coffee, concentrates, lamtoro, pineapple, rice, grass, palm, cassava, tofu lees, fish meal, bone meal from the provinces of Banten, Lampung, Jakarta and West Java. Isolation was done by plating the samples on agar medium, The mold have grown on media was identified. Feed that has been mixed with the extracts and powders plus mold inoculum was incubated. After 3=7 days incubation, colony forming unit (CFU) of the mixtures were counted. The results showed that the majority of feed contaminated with mold, but still below the threshold. The mold contamination in wheat flour, corn, concentrates and tofu lees exceeds from the threshold. Aspergillus sp, A. amstelodami, A. clavatus, A. Candidus, A. flavus, A. fumigatus, A. glaucus, A. niger, Cladosporium sp., Curvularia sp., Fusarium sp., Hyphomycetes sp., Mycelia sterilata, Mucor sp., Paecilomyces sp., Penicillium sp., and Rhizopus sp. Penicillium sp were most commonly found in the feed as much as 2.56 x 107 CFU. At a concentration of 10%. in vitro test showed that the piper betel leaf in powder form is more effective than extract form to inhibit the growth of A.flavus The conclusion of this study was flour, corn, concentrates and tofu lees contaminated by molds. Penicillium sp and 17 species of mold were the most frequently found compared to other fungi. Powders the best form of the piper betel as antimold. ABSTRAK Cemaran kapang pada pakan dan bahan penyusunnya adalah penting sebab kapang yang tergolong patogenik dan toksigenik dapat mencemari dan menyebabkan mikosis dan mikotoksikosis pada ternak sapi. Informasi mengenai kapang pencemar diperlukan dalam usaha pengendaliannya. Berdasarkan penelitian sebelumnya diketahui daun sirih (Piper betle) mempunyai aktivitas antikapang yang tinggi. Penelitian ini bertujuan untuk memperoleh data cemaran kapang pada pakan ternak sapi dan bahan penyusunnya dari propinsi Banten, Lampung, DKI Jakarta, dan Jawa Barat, serta menguji sirih sebagai obat herbal antikapang yang telah terpilih dari tanaman obat tradisional asli Indonesia. Isolasi dan identifikasi kapang telah dilakukan pada tepung, gliserida, onggok, jagung, kacang, kelapa, kopi, konsentrat, lamtoro, nenas, beras, rumput, sawit, singkong, ampas tahu, tepung ikan, tepung tulang dari provinsi Banten, Lampung, DKI Jakarta, dan Jawa Barat. Isolasi dilakukan dengan membiakkan sampel pada media agar, Kapang yang sudah tumbuh pada media diidentifikasi. Pakan yang telah dicampur dengan ektrak dan serbuk ditambahkan inokulum kapang, kemudian diinkubasi. Setelah 3-7 hari diinkubasi, dihitung colony forming unit (CFU) yang berkembang. Hasil penelitian menunjukkan bahwa sebagian besar pakan tercemar kapang, tetapi levelnya masih berada di bawah batas ambang. Cemaran kapang pada tepung, jagung, konsentrat, dan ampas tahu melebihi batas ambang. Kapang-kapang tersebut adalah Aspergillus sp, A. amstelodami, A. clavatus, A. candidus, A. flavus, A. fumigatus, A. glaucus, A. niger, Cladosporium sp., Curvularia sp., Fusarium sp., Hyphomycetes sp., Miselia sterilata, Mucor sp., Paecilomyces sp., Penicillium sp., dan Rhizopus sp. Kapang Penicillium sp adalah yang paling banyak ditemukan pada pakan yakni sebanyak 2,56.107 CFU. Uji in vitro menunjukkan bahwa daun sirih dalam bentuk serbuk lebih efektif dibandingkan bentuk ekstrak untuk menghambat pertumbuhan A. flavus pada konsentrasi 10%. Simpulan penelitian ini adalah tepung, jagung, konsentrat dan ampas tahu tercemar oleh kapang. Ditemukan 17 jenis kapang pencemar pakan dan kapang Penicillium sp yang paling banyak jumlahnya. Antikapang sirih yang terbaik adalah dalam bentuk serbuk.
Protein Rekombinan Outer Membrane Protein-31 dengan Superoxide Dismutase pada Pichia Pastoris Berpotensi Sebagai Kandidat Vaksin Brucellosis Arizah Kusumawati; Sri Kartika Wijaya; Ulfatul Husnaa; Yana Rubiyana; Adi Santoso
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (186.759 KB)

Abstract

Brucellosis is zoonotic disease caused by Brucella infections transmitted to human either direct contact with animals or consume their products. This becomes problematic in the world especially in endemic countries of Brucella and development countries. Brucella melitensis is one of pathogenic species from genus Brucella. Combining immunogenic side both the outer membrane protein 31 (OMP31) derived from B. melitensis and superoxide dismutase (SOD) derived from B. abortus has the potential to be developed as a candidate for recombinant vaccine. In this study, the expression protein of fusion OMP31-SOD inserted in pPIC9K plasmid with extra His-tag sequence, intends to be developed as a recombinant protein vaccine. Recombinant plasmids pPIC9K+OMP31-SOD were transformed into Pichia pastoris KM71 using electroporation method. Selection of transformants was as performed using geneticin® concentrations of 0.25 mg/mL – 4 mg/L Selected transformants were grown to express the OMP31-SOD recombinant protein. Protein expression was confirmed using both of dot-blot method and electrophoresis with Coomassie Brilliant Blue (CBB) staining. The OMP31-SOD recombinant protein was successfully expressed extracellular by Pichia pastoris KM71 clones 3, 4, and 6 with a molecular weight approximately 40 KDa.

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