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Jurnal Veteriner

jvet
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Published by Universitas Udayana

ISSN : 14118327 EISSN : 24775665 DOI : https://doi.org/10.19087/jveteriner

Core Subject : Health,

Veterinary

Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki

Arjuna Subject : -

Articles 1,116 Documents

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Heart Rate of Reticulated Pythons (Malayopython reticulatus) from Bali based on Its Body Length (DETAK JANTUNG ULAR SANCA BATIK (Malayopython reticulatus) BERDASARKAN PANJANG TUBUH DI BALI) Steven Dwi Purbantoro; Puveanthan Nagappan Govendan; I Gusti Made Krisna Erawan; Zefanya Christiani; Erika Erika; Yedija Putra Kusuma Wardana Rumbay; Slamet Raharjo
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study was aimed to determine the correlation between heart rate and body length of reticulated python (Malayopython reticulatus) from the island of Bali. The study offers data of the M. reticulatus heart rate value to exotic animal veterinary practitioner. Fifteen clinically healthy snakes, consists of 9 females and 6 males were used in this study. Heart rate was measured twice a day during day and night using a fetal Doppler (BF-500 eBestman®, China) and body length using a measurement tape. Heart rate was analyzed with independent t-test to time collection and correlation to body length. Results show that heart rate mean value at day and night of the M. reticulatus from the island of Bali was each 65.68±14.093 bpm and 64.53±13.637 bpm. Heart rate value of wild-caught snakes is higher (67.90±15.290 bpm) than captivebred snakes (60.92±10.039 bpm). Total length (TL), snout-vent length (SVL), and snout-heart length (SHL) mean value of M. reticulatus from the island of Bali was each 168.6±90.67 cm, 149.53±82.77 cm, and 38.7±19.28 cm. Correlation between heart rate and total length has higher R-value (R = 0.802), and followed by snout-heart length (R = 0.800) and snout-vent length (R = 0.792). Statistically, it could be concluded that heart rate during day and night time shows no significant difference, heart rate value based on how it is kept is significantly different, and heart rate was negatively correlated to body length.

A Polymorphism of Insulin-Like Growth Factor Binding Protein 2 Gene Associated with Growth and Body Composition Traits in Kampong Chickens Ahmad Furqon; Asep Gunawan; Niken Ulupi; Tuti Suryati; Cece Sumantri
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Insuline-like growth factor binding protein 2 (IGFBP2) is one of the principal binding proteins that has biological functions involved in growth, development, and differentiation. Selection for rapid growth based on molecular Marker Assisted Selection is required to increase production performance. The present study was designed to analyze the associations of IGFBP2 gene polymorphisms with chicken growth and body composition traits. Kampong chicken, a native chicken in Indonesia, is slow-growing chicken. A total of 59 males were used in the current study. Growth and body composition were measured in 24 wk of age. Primers for intron 2 region were designed from genomic chicken sequence. A c.1032C>T SNP of the IGFBP2 gene intron 2 region was detected and PCR-RFLP method was then used to genotype Kampong chicken population. The result showed that IGFBP2 polymorphism was significantly associated with body, carcass, breast, breast muscle, pectoralis minor, leg, and wings weight in Kampong chicken population (P < 0.05). The research suggests that the IGFBP2 gene could be a candidate gene that affects growth and body composition traits in chicken.

Akumulasi Timah Hitam dalam Daging dan Tulang Ayam Kampung dan Ayam Negeri (LEAD ACCUMULATION IN MEAT AND BONES OF DOMESTIC AND BROILER CHICKEN) Djohan .; Charles Rangga Tabbu
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (204.747 KB)

Lead is a heavy metal polluting the environment, and its accumulation in animal or human bodies canhave neurotoxic and nephrotoxic effects on animals and human. Lead-contaminated chicken meat can bethe source of lead to human. Lead exposure to human can be assessed by measuring its concentration andaccumulation in chicken body parts and analyzing chicken consumption patterns. This study was conductedto measure lead concentrations in chicken body parts and to estimate lead exposure caused by consumptionof chicken body parts (breast, legs, wings) and tissues (meat, skin, cartilage, spongy bones). Samples wereextracted by using aqua regia digestible method with a mixture of HCl: HNO3 (3:1; v/v) and leadconcentrations were measured by the Atomic Absorption Spectrophotometry (AAS) method. The leadconcentrations in chicken tissues varied from< 0.01 to 1.81?g.g-1dry weight.The average concentrations oflead in chicken tissues were lower than the recommended safety level of lead in chicken meat (1.0?g.g-1),except for the breast cartilage (1.03?g.g-1). The lowet accumulation level 2.6 ?g g-1 was found in domesticchicken wings while the highest of 32.9 ?g g-1 was found in broiler chicken breast (total of meat, skin,cartilage). Based on the data of lead accumulation in chicken tissues, a polynomial equation describing theprobability (P) to be exposed to certain amount of lead in chicken tissues (A, in ?g) was determined as P =-(1 x 10-3)A2 + (6,4 x 10-2)A.

Enzyme Linked Immunosorbent Assay Test for Antibody of Classical Swine Fever Virus In Timor-Leste (UJI ENZYME LINKED IMMUNOSORBENT ASSAY TERHADAP ANTIBODI VIRUS CLASSICAL SWINE FEVER DI TIMOR-LESTE) Rui Daniel de Carvalho; I Nyoman Suartha; Nyoman Sadra Dharmawan; I Made Kardena
Jurnal Veteriner Vol 17 No 4 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.015 KB)

The objective of this study was to evaluate the implementation of Classical Swine Fever (CSF)vaccination on pigs in Timor-Leste. The study was conducted by analyzing the percentage of CSF antibodyin pigs sera that obtained from pigs in four districts which were located in the hills and coast of Timor-Leste. Evaluation was also carried out by observing the dominant factor that affecting the increase ofantibody titers in the sera. A total of 240 pigs sera were taken before and after vaccination and thenchecked for antibodies against of CSF virus by using PrioCheck CSFV Ab ELISA kits (Prionics Ag). Twohundred and forty serums obtained from non-vaccinated pigs and 240 other serum obtained from the samepigs, after being vaccinated with CSF vaccine. Time interval from the first and the second serum collectionwas at least 14 days post-vaccination. The results showed there was a significant difference (P<0.01) forthe presence of antibody in vaccinated pigs compared with the unvaccinated. A total of 75% serum fromvaccinated pigs was found positive for the antibody containing, while only 16.7% of serum from nonvaccinatedpigs was positive. The odd ratio analysis showed that the most influential factor for theincrease of antibody titer against CSF virus was vaccination status. among the other factors of age, sexand geographical study.

Deteksi, Isolasi, dan Identifikasi Avian influenza Subtipe H5N1 pada Unggas di Pulau Jawa, Indonesia Tahun 2016 (DETECTION, ISOLATION AND IDENTIFICATION OF H5N1 SUBTYPE AVIAN INFLUENZA IN POULTRY IN JAVA ISLAND, INDONESIA, 2016) Dyah Ayu Hewajuli; Ni Luh Putu Indi Dharmayanti; I Wayan Teguh Wibawan
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

H5N1 subtype Avian influenza has harmed for poultry in Indonesia currently. Since clade 2.3.2 Avian influenza has been found in waterfowl from 2012, mortality of waterfowl has been occured until now. Change of extreme weather condition with high rainfall and flood, small poultry that does not adjust stricted biosecurity and without vaccination program, and chain sale of poultry on traders can increase case and transmission of Avian influenza in poultry. The objective of study was to isolate and identify Avian influenza on poultry in Central Java, West Java, East Java and Banten in 2016. The study was conducted by using Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) method to identify H5N1 subtype Avian influenza on poultry. The study was also conducted by isolation of H5N1 subtype Avian influenza from poultry into 9-11 days of embrionated chicken eggs. The study result represented that a number of 24 pool samples were positive reacted on RT-PCR with matrix primer, a total of 15 pool samples was positive reacted on RT-PCR with H5 primer and as many as 11 pool samples were positive reacted on RT-PCR with N1 primer. Two isolates of H5N1 subtype Avian influenza were isolated samples collected from the Serang City and six isolates of H5N1 subtype Avian influenza from Avian influenza case on waterfowl in Lamongan District. EID50 titer isolates of Avian influenza virus subtype H5N1 were > 108. The conclusion of this study was H5N1 subtype Avian influenza detected on samples collected from cloacal, trachea swab and organs of poultry from Pekalongan District, Brebes District, Serang City, and Lamongan District in 2016. H5N1 subtype Avian influenza can be grown and isolated from samples collected of Serang City and Lamongan District in 2016. H5N1 subtype Avian influenza can be detected, isolated, and indentified in poultry in Java Island, Indonesia in 2016.

Estimation of Aflatoxin M1 Exposure through Consumption of Various Dairy Milk Products in Yogyakarta, Indonesia (ESTIMASI PAPARAN AFLATOKSIN M1 MELALUI KONSUMSI BERBAGAI PRODUK SUSU DI YOGYAKARTA, INDONESIA) Ika Sumantri; Fitri Purwanti; Nuryono Nuryono; Ali Agus
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

This study was conducted to investigate the occurrence of aflatoxin M1 (AFM1) in various milk products marketed in Yogyakarta Province (Indonesia) and to estimate the exposure of aflatoxin through contaminated milk consumption. Fresh milk (n=20), pasteurized milk (n=16), and recombined milk products (n=6) were sampled for AFM1 concentration testing by a competitive ELISA test using ELISA kit for AFM1 assay. A survey was conducted to interview consumers (n=88) on milk consumption habit (milk type and amount of consumption). ELISA assays showed 92.5% of samples werecontaminated with AFM1 in a range of 24-570 ng/L (average: 216 ng/L). The highest average AFM1 concentration was detected in pasteurized milk sample (244 ng/L), followed by fresh milk (219 ng/L), and the lowest was in recombined milk sample (131 ng/L). However, 100% of recombined milk samples had AFM1 concentration >50-500 ng/L. Thus, recombined milk product was most likely the main source of AFM1 intake due to its high daily consumption in all age groups. Based on AFM1 levels found in milk and consumption of corresponding milk sample, it was estimated that the overall AFM1 exposure ranges from 1.23 ng/kg body weight/day (in 6-15 year-old children) up to 5.26 ng/kg body weight/day (in 3-5 year-old children). In conclusion, this study revealed high occurrences of AFM1 dairy milk marketed in Yogyakarta. Although levels of AFM1 contamination were in Indonesian regulatory limit, high exposure of aflatoxin found in all age groups of consumer. Thus, this preliminary study provides evidence that AFM1 contaminated milk is a serious public health hazard in Indonesia.

Karakterisasi Lactobacillus spp. yang Diisolasi dari Susu Kambing Etawa untuk Pengembangan Probiotik (CHARACTERIZATION OF LACTOBACILLUS SPP., ISOLATED FROM MILK OF ETAWA GOATS FOR LOCAL PROBIOTIC DEVELOPMENT) Putu Rima Sintyadewi; Yan Ramona; I Nengah Sujaya
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (370.683 KB)

The objective of this research was to characterize Lactobacillus spp., isolated from milk of Etawagoats for local probiotic development. Total of 23 isolates Lactobacillus spp. were tested for resistance tolow pH conditions, high levels of natrium deoxy cholate, and modified gastric juice conditions. Besidesthat, those isolates were also tested to convert cholic acid (CA) into deoxycholic acid (DCA). Isolate thatshowed the most potential properties for local probiotic development was identified by 16S rDNA analysisusing following amplification of this sequence with primers of 27F and 520R). The results showed that 12isolates were found to be resistant to low pH conditions and to high level of NaDC (0.6 mM). Three of them(Lactobacillus spp. GMA46, Lactobacillus spp. GMA47 and Lactobacillus spp. GMA50) did not convertcholic acid into deoxy cholic acid, indicating that they are safe for human use. Lactobacillus spp. GMA46showed better performance in the gastric juice (a model of gastic and intestinal juice containing pepsin andpancreatin enzymes at pH 2, 3 and 4) simulation test. This GMA46 isolate was identified as L. caseiATCC 334 and L. paracasei subsp. tolerans strain NBRC 15906 with 100% similarity, in term of its 16srDNA nucleotide sequence. The results of this research indicate that Lactobacillus sp. GMA46 is anIndonesian potential probiotic strain, isolated from milk of etawa goats

Pola Pewarisan Kaki Rengket Secara Autosomal Resesif dan Koefisien Inbreeding pada Ayam Pelung di Cianjur (THE AUTOSOMAL RECESSIVE IN CROOPER TOES INHERRITANCE PATTERN AND INBREEDING COEFFICIENT IN PELUNG CHICKEN AT CIANJUR) Budi Setiadi Daryono; Miftahul Mushlih
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (184.026 KB)

Pelung Chicken is classified as dual-purpose chicken (meat and singer). Physical abnormalities affectthe selling price. One of the common physical abnormalities is crooked toes. The aims of this study were todeterminate the crooked toes inheritance pattern and the value of inbreeding coefficient (IC) in Pelungchicken. The method that used in this study is descriptive-explorative and samples were taken usingpurposive sampling analysis. The research was conducted at Cibeber, Cianjur district, West Java.inbreeding coeficient was determined using F = “ (1/2)n+1 (1+Fc) formula and inheritance pattern wasdetermined by the pedigree resulted and chi-square test goodness of fit test. The results show that aCreeper abnormality is inherited as autosomal recessive with the IC value reaches 0.6. The highest valueof IC shows highest risk probability of crooked toes in pelung chicken.

Karakteristik Gen Sitokrom C Oksidase Sub Unit I (CO1) Lebah Liar Apis cerena (Hymenoptera: apidae) Asal Pulau Hoga Sulawesi Tenggara Suriana Suriana; Jamili Jamili; Parakkasi Parakkasi
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

The study was conducted to assess the caracteristic of cytochrome C oxidase subunit I (COI) gene on wild honey bee Apis cerena, and detection of barcode sites from these gene. A total fifteen individual A. cerena were collected from Hoga Island, Southeast Sulawesi. Genomic DNA was extracted from torax, then amplified by PCR method and than sequenced. Sequencing result characterized their nucleotide and amino acid content. The results showed that 595 nucleotides at the 5' end of COI gene of A. cerena very conserved at the most of the sites. Nucleotide dominated by thymine and adenine bases (± 70%). There are 25 barcoding sites for A. cerena. There are two of these sites are diagnostics for A. cerena from of the Hoga Island. From of 595 basepairs nucleotide were prediction encode 198 amino acid, and only eleven percent of the these are varied between species. Amino acid 91th (treonine) and 103th (asparagin) were diagnostics amino acid for A. cerena from Hoga Island, Southeast Sulawesi. Molecular phylogeny reconstructed based on both nucleotide and amino acid sequence placing apis cerena from Hoga Island, Souteast Sulawesi is closely related to Apis cerena from Genbank source.

An Amino Acids on Bali Cattle and Wagyu Beef Based on Different Function of Muscle (ASAM-ASAM AMINO SAPI BALI DAN DAGING SAPI WAGYU BERDASARKAN FUNGSI OTOT YANG BERBEDA) I Nengah Kerta Besung; Rasdianah Rasdianah; I Wayan Suardana; Ni Ketut Suwiti
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Beef is an essential source of protein and several functional compounds that are very important for human. The quality of beef depends on both genetic and environmental factors like feed, age, sex, and others. This research aimed to determine the composition of amino acids both Bali and Wagyu beef on the different activity of muscle, i.e. active and passive. As many as 5 g of each sample was used in this study. The active beef samples were presented by Biceps femoris, and passive beef samples were presented by Longissimus dorsi. High-Performance Liquid Chromatography (HPLC) method was used in order to an identification of amino acids according to the standard procedure. Results of the study showed that the essential amino acids content both bali cattle and wagyu were higher than non-essential, and amino acids content originated from active muscle was higher than passive muscle. Methionine, phenylalanine, and serine on bali beef cattle were lower than wagyu beef. Overall, the content of amino acids essential was lower than non-essential. In conclusion, there is no significant difference of amino acids content both bali cattle and wagyu beef, but the function of muscle (active or passive) were known contribute to the difference of amino acids content.

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I G. Made Krisna Erawan

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Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

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Editorial Address Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

Location Kota denpasar, Bali INDONESIA

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Contact Name
I G. Made Krisna Erawan

Contact Email
krisnaerawan@unud.ac.id

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Journal Mail Official
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Editorial Address
Animal Hospital, Faculty of Veterinary Medecine Building, Udayana University, 2nd Floor, Jalan Raya Sesetan, Gang Markisa No 6, Banjar Gaduh, Sesetan, Denpasar, Bali, Indonesia

Location
Kota denpasar,

Bali

INDONESIA