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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Profil Saraf Nitrergik Sekum Ayam Pedaging yang Diinfeksi Eimeria tenella (THE PROFILE OF NEORAL NITRERGIC IN THE COECUM OF BROILER INFECTED WITH Emeria tenella) Amelia Hana; Pudji Astuti; Yuda Heru Fibrianto; Sarmin .; Claude Mona Airin
Jurnal Veteriner Vol 16 No 4 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Neurotransmitter found in the enteric nervous system that plays an important role in a variety ofenteropathies, including inflammatory bowel disease. Alteration of nitrergic neurons has been reported to bedependent on the manner by which inflammation is caused. This study was performed to determine theprofile of neural nitrergic with Nicotinamide Adenine Dinucleotide Phosphate-diaphorase (NADPH-d)staining in the cecum of broiler infected by Eimeria tenella (E. tenella). Thirty six health free of coccidiosisbroiler one-day old chickens (DOC) used in this study. All of broiler adapted for 7 days with food and drinkgiven ad libitum then divided into three groups, each group consisted of 12 broilers. The 1st group wascontrol only given orally 1.0 ml of distilled water/ heads , while 2nd group was infected with a single dose of5x103 oocysts/ head orally, and 3rd group was infected with a single dose of 2x104 oocysts/ head orally. Onday 7 post infection, all of chickens were fasted for 12 hours then were euthanized and cecum was taken.Lesion score of cecum was assessed. Furthermore, tissue of the coecum was prepared for hisochemicalusing NADPH-d staining to determine morphology, and morhometric of nitrergic neurons. The resultshown that cecum lesion score of group I is 0, group II is +2, group III is +3. Neuron density in the cecum ofgroup I is 2794.96+4.65 neuron/cm2, group II is 4930.36+63.73 neuron/cm2 and group III is 7892.31+44.97 neuron/cm2. Neuron density of group II and III increased signifcantly (p<001) than group I. Nitrergic neurondesity of group III increased significantly (p<0.05) than that of group II. It was concluded that the infectionof E.tenella led to increase the number of neutrl mienteric nitrergic of the cecum. The higher lesion score ofcecum led to increase the number of mienteric nitrergic neuron.
Phytochemical Content and Protective Effect of Kleinhovia hospital Leaves Extract on Pancreatic Cytotoxicity in Hyperglycemic Rats Yuliana Yuliana; Sianny Herawati
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Diabetes mellitus is a wide problem nowadays. Many traditional plants are used to overcome thecomplication of this disease. One of them is paliasa (Kleinhovia hospital Linn) leaves. This study aimswere to investigate phytochemical contents and the protective effects of paliasa leaves extract on pancreaticcytotoxicitybased on microscopic lesion such as hemorrhagic score and necrotic appearances, in alloxaninducedhyperglycemic rats. Male Wistar rats (three months old) were divided into four groups consistedofseven rats each. Group I was diabetic control, Group II was diabetic groups, paliasa extract (300mg/kgBW) were given by sonde for a period of 14 days prior to alloxan injection (150 mg/kg intraperitoneal).Group III was diabetic rats given 600 mg/kg BW paliasa extract and group IV was diabetic rats given 900mg for 14 days. At the end of the study, rats were sacrificed. Tissue sample ofpancreaswas then processedfor slide preparation and was stain with hematoxylin eosin.Pancreas hemorrhagic score was divided intofour criteria, focal(score 1), multifocal (score 2), extensive (score 3), and difuse (most severe, score 4).Microscopic examination was done using binocular microscope, at Laboratory ofVeterinaryPathology,Disease Investigation Centre Denpasar, Bali. Data werethenanalyzed by using analysis ofvarians. Study showed that paliasa extract could lower hemorrhagic score on pancreas of diabetic rats,even though it was not significant compared to control group (p 0,205). Phytochemical analysis showedthat paliasa leaves extract contained alkaloid, terpenoid, and flavonoid. Necrotic appearances were variedfrom pycnosis, karyorheksis, karyolysis, and vacuolization. In conclusion, paliasa leave extract may haveprotective effect on pancreas cytotoxicity.
GAMBARAN DARAH DAN KALSIUM DOMBA LOKAL (Ovis aries) YANG DIIMPLANTASI BIFASIK KALSIUM FOSFAT 70/30 SCAFFOLD 3D BERPOROS Dwi Utari Rahmiati; Gunanti Gunanti; Eva Harlina; Kiagus Dahlan
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (121.823 KB) | DOI: 10.19087/jveteriner.2018.19.4.547

Abstract

The aim of this research is to study the biocompatibility biphasic calcium phosphate with alginate combination by evaluating blood and calcium concentration. This study used three male sheeps, age 1.5 year old with an average weight of 20 kg. Anaesthesia is using an intravenous anaesthetic combination of 10% ketamine HCl (2 mg / kg BW) and 10% xylaxine HCl (0.1 mg / kg BW). The implant material is grown on the right sheep tibia. As a control, the left sheep tibia was made of defects but not implanted. The incision orientation is the straight line of the tibial femoral joint at medial, 3.6 cm from caudal to cranial and perpendicular from that point 5.7 cm to the distal direction. The defect is made using a bone drill to make a hole according to the size of the implant (diameter 0.8 cm and 0.4 cm long). Blood parameters (erythrocytes, hemoglobin (Hb), hematocrit (Hct), total leukocytes and differentiation of leukocytes; neutrophils, eusinophils, basophils, lymphocytes and monocytes) and calcium evaluations were performed on days 0, 7, 30, 60 and 90 post implantation. Based on complete blood count no significant of blood parameters. Based on the calcium value, there are significant values on the 30th and the 90th. Blood calcium concentration were decreased from day 0 to 90.
Kemampuan Ekstrak Jamur Lingzhi dalam Menghambat a-Glucosidase dan Menurunkan Kadar Gula Darah pada Tikus Hiperglikemia (THE ABILITY OF LINGZHI MUSHROOM EXTRACT (GANODERMA LUCIDUM) IN INHIBITING a-GLUKOSIDASE AND ITS EFFECT ON HIPERGLYCEMIAOF RATS) Ratih Dwi Indriani; I Nyoman Suarsana; I Wayan Sudira
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Lingzhi mushrooms has been widely used by community empirically for the traditional medicine forlowering blood glucose levels. The purpose of this study was to determine the ability of the lingzhimushroom (Ganoderma lucidum) extract to inhibit á-glukosidase hyperglycemia in rat. Lingzhi mushroomethanol-extract were tested for their inhibition against á-glucosidase using spectrophotometry method. Atotal of 25 rats used in this study were divided into five groups. Four groups of rats (P1-P4) were given 2 mlof sucrose 80% orally, and one group of rats (P0) as a negative control. Ten minutes before giving sucrose,three groups (P2-P4) of rats were given the lingzhi mushroom extract of 1 ml orally at a dose of 10%, 20%,and 30% respectively, while the second groups (P1) as a positive control of hyperglycemia. After that in allgroups were examined the blood glucose levels at 0, 10, 30, 60, 90, and 120 minute after sucroseadministration. The results showed, lingzhi mushroom extract has the ability to inhibit á-glucosidasewith IC50 value of 162,01 ìgmL-1. The result of blood glucose test showed that administration of lingzhimushroom extract at a dose of 30% faster lowering blood glucose levels compared with a dose of 10% and20%.
Vaksin Kombinasi Newcastle Disease dengan Avian Influenza Memicu Imunitas Protektif pada Ayam Petelur terhadap Penyakit Tetelo dan Flu Burung (COMBINED NEWCASTLE DISEASE (ND) AND AVIAN INFLUENZA (AI) VACCINES INDUCE PROTECTIVE IMMUNE RESPONSE IN COMMERCIA Gusti Ayu Yuniati Kencana; I Nyoman Suartha; Ni Made Ayu Sintya Paramita; Arini Nur Handayani
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Newcastle disease (ND) and Avian Influenza (AI) are infectious diseases and still endemic in Indonesia.Prevention of the disease is conducted by vaccination of birds as the source of the infection. The use ofcombined ND-AI vaccine is expected to be able to prevent both diseases simultaneously. This study aimwas to determine the potency of combined ND-AI vaccine in field condition. Field trial vaccination wasconducted in commercial layer chickens in Tabanan Bali, and the HI test was conducted at the Faculty ofVeterinary Medicine Udayana University, Denpasar. Field trial in commercial layer chickens showed thatthe average HI titer of ND sera from pre-vaccinated chickens was 22.7HI units and AI titer was 21.27 HIunits. The ND titers increased to 25.47 HI Unit, 27.0 HI units, and to 28.73 HI units, whereas AI titersincreased to 27.93 HI Unit, 28.53 HI units, and 28.47 HI units in two, three and four weeks post-vaccinationwith the ND-AI combined vaccine, respectively. Statistically, based on ND and AI antibody pre and postvaccination,it is indicated that the combined ND-AI vaccine was able to induce immune response higherthan the protective titer level (>24). Period of collecting the sera samples also affected the titer of NDVand AI antibodies (P<0.01). Therefore it is recommended that vaccination should be conducted at antibodytiter of < 4 HI Unit.
Hipotermia dan Waktu Pemulihannya dalam Anestesi Gas Isofluran dengan Induksi Ketamin-Xylazin pada Anjing (HYPOTHERMIA AND ITS RECOVERY IN GAS ISOFLURANE ANESTHESIA WITH KETAMINE-XYLAZINE INDUCTION ON DOGS) Gagak Donny Satria; Setyo Budhi; Dinni Nurdyanti
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The most common effect occurred during anaesthesia is the decrease of body temperature. Technologicaldevelopment has enabled the used the latest innovations in order to to increase the efficacy and the safetyof anaesthesia. The purpose of this study was to determine the effect of ketamine-xylazine injection onhypothermia and its recovery at dog which anesthetized with isoflurane. Ten healthy dogs were dividedinto two groups with each group consisted offive dogs. In Group A, dogs were given premedication (atropinesulfate 0.04 mg/kg) and then anaesthetized with isoflurane gas (4% for induction dose and 1% for themaintenance dose). In Group B dogs were given premedication atropine sulfate (0.04 mg/kg) and ketamineHCl induction solution (10 mg/kg) mixed with xylazine HCl (2 mg/kg), and anaesthetized with isofluranegas (maintenance dose of 1%). Adaptation period was conducted in one week. Body temperature wasmeasured before, during, and after the duration of anaesthesia. The data was analyzed statistically by arepeated Anova test. This study found that the mean body temperature of dogs in Group A decreased from37,88±0,51 oC to 34,64±0,95 oC over a period of anaesthesia, and the recovery time was over 40 minutespost-anaesthesia. In Group B, body temperature decreased from 38.06±0.42 oC to 34.96±1.23 oC, and therecovery time was 90 minutes. In conclusion, the use of ketamine-xylazine in isoflurane anaesthesiaprocedures on dogs, would need post-anaesthesia preparation procedure regarding with hypothermia andits recovery.
KONDISI NEONATUS ANAK BABI YANG DILAHIRKAN OLEH INDUK YANG DISUNTIK GONADOTROPIN SEBELUM PENGAWINAN Friska Mery Montolalu; Anita Esfandiari; Damiana Rita Ekastuti; Wasmen Manalu
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (94.373 KB) | DOI: 10.19087/jveteriner.2018.19.1.76

Abstract

Injections of sows with pregnant mare serum gonadotrophin (PMSG) and human chorionic gonadotrophin (hCG) prior to mating have been shown to improve endogenous secretions of pregnant hormones that improve and optimize the uterine environment and condition during pregnancy that eventually lead to give birth to superior piglets. This experiment was designed to study the effects of PMSG and hCG injections of sows prior to mating on prenatal growth as indicated by the conditions of the the neonate piglets. Thirty mature female Landrace pigs were divided into 2 groups i.e., 15 pigs were injected with PMSG and hCG prior to mating, and the other 15 pigs were injected with physiological NaCl solution as a control. Before mating, the estrus cycles of the experimental sows were synchronized by injecting prostaglandin two times with 14 days interval. The experimental pigs were injected with PMSG and hCG or 0.90% NaCl solution at the same time with the second prostaglandin injection and were further mated naturally. During pregnancy, the experimental sows were maintained with the normal management condition. The results showed that piglest born to sows injected with PMSG and hCG prior to mating had shorter and faster birth process with higher activities and survivals as compared to those born to control sows. Piglets born by sow that were injected with PMSG and hCG before mating had a more optimum birth weight accompanied by greater body length, limb height, and higher rear limb height. Piglest born to by sows that were injected with PMSG and hCG prior to mating showed superior conditions that would contribute to optimum and superior preweaning and postweaning growths until maturity.
Profil Elektrolit Serum Pedet Sapi yang Diinfeksi Escherichia coli K-99 dan Diberi Mikrokapsul Imunoglobulin-G Anti E. coli Arief Purwo Mihardi; Anita Esfandiari; Sus Derthi Widhyari; Sri Murtini
Jurnal Veteriner Vol 20 No 2 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (58.791 KB) | DOI: 10.19087/jveteriner.2019.20.2.158

Abstract

Escherichia coli (E. coli) K-99 infection cause acute diarrhea in calves which may reduce electrolyte, such as sodium and potassium. The experiment was conducted to study the sodium and potassium profiles in calves infected by E. coli and received immunoglobulin G anti E. coli K99 microcapsules. Sixteen calves, 4 – 6 days years old, were grouped into four groups, consists of four calves, i.e. negative control/NC group (the calves were not infected by E. coli K-99 and no treatments); positive control/PC group (the calves were infected by E. coli K-99 and no treatments); treatment 1/P1 group (the calves were infected by E. coli K-99 and received colostrum suspension of IgG anti E. coli K-99); and treatment 2/P2 group (the calves were infected by E. coli K-99 and received microcapsules of IgG anti E. coli K-99). Escherichia coli K-99 were infected to all calves except the NC group orally with the dosage of 5 × 1010 cfu/ml. Suspension and microcapsules of IgG anti E. coli K-99 for each calf were given orally with dosage of 1.76 gram IgG anti E. coli K-99 per day. Serum were collected from jugular vein at 0, 24, 48, 72, and 168 hours after E. coli K99 infection. The sodium and potassium concentrastion were analyzed using a spectrophotometer. Results of the experiment indicated that the concentration of sodium and potassium were not significantly different among groups. Sodium concentration of P2 group was relatively higher than PC or P1 and showed increased concentration 168 hours after infection. Potassium concentration of P2 were higher than the PC group, but lower than NC and P1 group. In conclusion, the administration of IgG anti E. coli K-99 microcapsules was not significantly change the sodium and potassium concentration, but may maintain the stability of sodium level in calves infected by E. coli K-99.
Keragaman Spesies dan Genetik Bakteri Staphylococcus pada Ikan Tuna dengan Analisis Sekuen 16s rRNA (SPECIES DIVERSITY AND GENETIC OF STAPHYLOCOCCUS BACTERIA IN TUNA FISH BY USING 16S rRNA SEQUENCE ANALYSIS) Putu Mei Purnama Dewi; I Nengah Kerta Besung; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.064 KB)

Abstract

Tuna industry belongs to high economic value fish of marine fisheries commodity. Bacteria found intuna might have impact on consumer health. The purpose of this research was to determine the geneticvariation of Staphylococcus in Kedonganan fish market, Kuta, Badung, Bali by sequence analysis of 16SrRNA. Staphylococcus bacteria was isolated from feces samples of 30 tuna. After identification with Gramstaining, Staphylococcus colonies were transferred to the medium chelex 10% for DNA extraction, 16SrRNA gene amplification by polymerase chain reaction (PCR), and electrophoresis. PCR results weresequenced, and the sequence obtained was edited using MEGA 5 and then BLAST was applied to confirmthe species. Genetic variation was determined by analysis of polymorphic sites with MEGA 5. The identifiedbacteria were Staphylococcus sciuri and Staphylococcus haemolyticus. Genetic distance of two isolates S.sciuri are close. The Other species that were identified were Enterococcus faecalis and Macrococcus caseolyticus.Conclusion on this research is Staphylococcus bacteria and two species S.sciuri have many variation.
Profil Progesteron Air Susu dan Tingkat Kebuntingan Sapi Perah Pascasinkronisasi Estrus Menggunakan Prostaglandin F2Alfa atau Progesteron-CIDR (MILK PROGESTERONE PROFILE AND PREGNANCY RATE ON DAIRY CATTLE AFTER ESTROUS SYNCHRONIZATION WITH PROSTAGLANDIN F Novi Suprihatin; Ligaya ITA Tumbelaka; Mohamad Agus Setiadi
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.524 KB)

Abstract

Research on estrus synchronization, progesterone profiles of post-synchronization and early pregnancyhas been conducted using 16 Holstein Frisian dairy cows. Treated cows were divided into 2 groups. Cowsin group I were synchronized with double injection of prostaglandin F2á (PGF2á, Lutalyse®) 25 mg / head at11 days apart, then were inseminated twice at 72 hours and 96 hours after the second injection of PGF2á(FTAI = fixed timed insemination). Group II were synchronized with progesterone implant-CIDR® for 11days. At the time of progesterone implant withdrawal the animals were injected with PGF2á at 25 mg /head then inseminated twice at 48 hours and 72 hours. Milk samples were collected on the day before, atthe treatment day and at day 1st, 3rd, 5th, 7th following the first insemination in order to determine theprofile of progesterone after synchronization, while for early pregnancy examinations, sampling of milkwere collected at day 21th, 24th and 27th after the first insemination. The milk samples were analyzed byRadioimmunoassay (RIA) method. Rectal palpation to confirm pregnancy was undertaken at day 60thafter the first insemination. The results showed a marked decrease in milk progesterone (nmol / l) at thefirst insemination (H0) in both group PGF2á and CIDR® (0.84; 0.49 vs. 0.92; 0.32), which indicated theoccurrence of estrus. Gradually increased of milk progesterone level (0.52; 0.68; 1.17; 1.69, respectively)started from day 1st, 3rd, 5th, 7th was seen in animals PGF2á group, whereas in the CIDR® group the milkprogesterone level was found fluctuate (0.21; 0.39; 0.33; 1.61). However, at day 7th the concentration ofprogesterone in both groups was significantly increased which indicated functional activity of the corpusluteum. Meanwhile the progesterone concentrations (nmol/l) of pregnant cows at day 21st, 24th and 27th ingroup PGF2á were 3.63; 3.51; 1.58 and in CIDR® group were 2.50; 2,79; 4.35, respectively. In non-pregnantcows, the progesterone concentrations (nmol/l) were lower (0.63; 0.42; 1.41 vs. 0.20; 0.27; 1.33), than thoseof pregnant cows. The results of rectal palpation after 60 days of the first Artificial Insemination (AI)confirmed that 5 cows with higher milk progesterone concentrations at day H21, H24, H27 from the firstinsemination were pregnant, with the possibilities at 62.5% in each group. It is concluded that estroussynchronization using either PGF2á or CIDR® in lactating dairy cows will give the same response and thiscould be detected using the milk progesterone profiles. Measurement of milk progesterone concentrationsby RIA began at day 21 of the first AI was effective for early pregnancy diagnosis.

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