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Jurnal Veteriner
Published by Universitas Udayana
ISSN : 14118327     EISSN : 24775665     DOI : https://doi.org/10.19087/jveteriner
Core Subject : Health,
Veterinary
Jurnal Veteriner memuat naskah ilmiah dalam bidang kedokteran hewan. Naskah dapat berupa: hasil penelitian, artikel ulas balik (review), dan laporan kasus. Naskah harus asli (belum pernah dipublikasikan) dan ditulis menggunakan bahasa Indonesia atau bahasa Inggris. Naskah ilmiah yang telah diseminarkan dalam pertemuan ilmiah nasional dan internasional, hendaknya disertai dengan catatan kaki
Arjuna Subject : -
Articles 1,116 Documents
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Kafein dalam Medium Maturasi Meningkatkan Fertilisasi dan Menekan Frekuensi Polispermi Oosit Domba dengan Maturasi Diperpanjang (CAFFEINE SUPLEMENTATION IN MATURATION MEDIUM IMPROVE NORMAL FERTILIZATION AND REDUCED THE FREQUENCY OF POLYSPERMY IN SHEEP OOC Reski Adelia; Ni Wayan Kurniani Karja; Mohamad Agus Setiadi
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (110.394 KB) | DOI: 10.19087/jveteriner.2017.18.3.337

Abstract

The objective of this study were to determine kinetic of nuclear maturation and the efficacy of caffeine suplementation in maturation medium on fertilization rate of sheep oocytes in vitro. In the first experiment, oocytes were matured for 16 (M-16), 20 (M-20), 24 (M-24), 28 (M-28) h to assessed the kinetic of oocytes nuclear maturation. In the second experiment, oocytes were matured for 24 h (M-24) or 28 h (M-28) without (M-24 or M-28 groups) or with caffeine suplementation at 4 h before the end of maturation period of oocytes matured for 24 h (M24-Kaf-4) and 28 h (M28-Kaf-4), or 8 h (M28-Kaf-8) before the end of maturation period of oocytes matured for 28 h. Result of the first experiment, 27.6% of oocytes were in metaphase II (MII) at 16 h. The percentage of MII oocytes significantly increased after 20 h (44.8%) to 24 h (88.9%) of maturation period (P<0.05), but the increasing was not found when the maturation period was prolonged until 28 h (89.3%) (P>0.05). However the number of oocytes with two pronucleus (2PN) was higher in group M-24 compared than that of M-28 group (P<0,05) and incidence of polyspermy increased in oocytes of M-28 group (P<0,05). No significant diferences was found in the total of oocytes fertilized among the group except of group M28-Kaf-8 (P>0,05). When caffeine was suplemented at 4 h before the end of maturation period a significantly reduced the incidence of polyspermy and increased the number of oocytes with 2PN in oocytes of M-28 group (P<0.05). In conclusion, the kinetic of nuclear maturation in sheep oocytes showed there was a variation in time required by oocytes to reach MII phase and caffeine improve normal fertilization and reduced the frequency of polyspermy on oocytes when the maturation period prolonged. ABSTRAK Penelitian ini bertujuan untuk mengetahui kinetika maturasi inti dan efektivitas suplementasi kafein pada medium maturasi terhadap tingkat fertilisasi oosit domba secara in vitro. Penelitian tahap I oosit dimaturasi selama 16 (M-16), 20 (M-20), 24 (M-24), dan 28 (M-28) jam untuk mengevaluasi kinetika maturasi inti oosit. Penelitian tahap II, oosit dimaturasi selama 24 jam (M-24) dan 28 jam (M-28) tanpa kafein (kelompok M-24 dan M-28) atau dengan penambahan kafein pada empat jam sebelum akhir periode maturasi pada oosit yang dimaturasi selama 24 jam (M24-Kaf-4) dan 28 jam (M28-Kaf-4), atau delapan jam (M28-Kaf-8) sebelum akhir periode maturasi pada oosit yang dimaturasi selama 28 jam. Hasil penelitian tahap I menunjukkan, 27,6% oosit berada pada tahap metafase II (MII) pada jam ke-16. Persentasi oosit MII meningkat secara signifikan setelah jam ke-20 (44,8%) hingga jam ke-24 (88,9%) periode maturasi (P<0,05) akan tetapi tidak ditemukan adanya peningkatan ketika periode maturasi diperpanjang hingga 28 jam (89,3%) (P<0,05). Namun demikian, jumlah oosit dengan dua pronukleus (2PN) lebih banyak pada kelompok M-24 dibandingkan dengan kelompok M-28 (P<0,05) dan kejadian polispermi meningkat pada oosit kelompok M-28 (P<0,05). Tidak ditemukan adanya perbedaan yang signifikan pada tingkat fertilisasi total antar perlakuan kecuali pada kelompok M28-Kaf-8 (P<0,05). Ketika kafein ditambahkan pada empat jam sebelum akhir periode maturasi secara signifikan dapat menurunkan kejadian polispermi dan meningkatkan jumlah oosit 2PN pada kelompok M-28 (P<0,05). Dapat disimpulkan bahwa kinetika maturasi inti oosit domba menunjukkan ketidakseragaman waktu yang dibutuhkan oleh oosit untuk mencapai tahap MII dan kafein dapat meningkatkan fertilisasi normal dan menurunkan frekuensi polispermi pada oosit ketika periode maturasi diperpanjang
Prevalensi dan Pola Gen Extended Spectrum B-lactamase Bakteri Usus Sapi Perah dan Penduduk Sekitar Peternakan di Surabaya Triffit Imasari; Wiwiek Tyasningsih; Eddy Bagus Wasito; Kuntaman Kuntaman
Jurnal Veteriner Vol 19 No 3 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (126.766 KB)

Abstract

Since identified in 1980s, the multiple drug resistant organisms such as Extended Spectrum Beta Lactamases (ESBL) producing bacteria is increasing. These bacteria Enterobacteriaceae strain are mostly resistant against third and also fouth generation cephalosporin. ESBL-producing bacteria are identified in both of human, environment and also in animal. There are three main ESBL genes that are commonly found namely SHV, TEM and CTX-M. The aims of this study were to explore the prevalence and pattern of ESBL gene among dairy cows and people around the farm. The faecal samples were collected from dairy cows and people around the farm, cultured on MacConkey agar supplemented with cefotaxim 1 mg/L, incubated at 37oC for 24 hours. Then the growing colony were tested for ESBL producer by Double Disk Synergy Test (DDST), then followed by Polymerase Chain Reaction (PCR) for ESBL gene. Total sampling technique with inclusion and exclusion criteria, Total 49 samples were collected, consisting of 25 dairy cows faeces and 24 people faeces. Among these, were identified 18 samples (72%) positive in dairy cows and 19 samples (79.1%) positive results in the people around the dairy farm. The ESBL gene, SHV, TEM, CTX-M were identified dairy cows were zero for SHV, TEM (12%), CTX-M (72%) while in people around the farm SHV (25%), TEM (16.7%), CTX-M (66.7%). There were significant different (p < 0.05) between dairy cows and people around the farm, of SHV ESBL gene and not different (p>0.05) of TEM and CTX-M ESBL gene respectively. The ESBL genes have spread among dairy cows and people around the farm.
Pola Kedatangan Serangga pada Jasad Hewan Sebagai Indikator dalam Kegiatan Forensik (INSECT ARRIVAL PATTERN ON CARRION AS AN INDICATOR OF FORENSIC ACTIVITIES) Supriyono Supriyono; Susi Soviana; Upik Kesumawati Hadi
Jurnal Veteriner Vol 20 No 3 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (303.916 KB) | DOI: 10.19087/jveteriner.2019.20.3.418

Abstract

Decomposition stage of carrion will attract various species of insects to come. Some species of insect will attract on carrion in the early stage of decomposition, but some of them in the late stage of death. The purpose of this research were to observe and analyze the distinctive features of insect succession on carrion that could predict the time of death. Two carrions were placed in indoor and outdoor. Insect collection and observation was done three times a day i.e, morning, afternoon and evening. Adult flying insects were collected by sweeping net, whereareas immature insect with manual. The result showed that decomposition of the carrion indoor were faster than the corrion outdoor. In outdoor there were found orders of Diptera (i.e Muscidae, Calliphoridae, Sarcophagidae,Tachinidae), Coleoptera (Chrysomelidae, Staphylinidae, Scarabeidae, Silphidae), Hymenoptera (Formicidae), Hemiptera, Blataria and Orthoptera (Grillidae). However, in indoors there were found the order of Diptera (Muscidae, Calliphoridae, Sarcophagidae, Tachinidae), Coleoptera (Chrysomelidae, Staphylinidae, Scarabeidae, Silphidae), Hymenoptera (Formicidae), Hemiptera, Aranea, and Lepidoptera. Decomposition stage of carrion indoor faster than outdoor. In the early stage to the decay stage, insects that came on carrion outdoor and indoor were Diptera (Calliphoridae, Tachinidae, Muscidae, and Sarcophagidae. On the post decay and skeletonization stage the insect that come were Coleoptera (Staphylinidae, Chrysomelidae, Scarabeidae, and Silphidae ). Hymenoptera (Formicidae) came from early stage to skeletal stage.
Perlindungan Protein Menggunakan Tanin dan Saponin Terhadap Daya Fermentasi Rumen dan Sintesis Protein Mikrob (PROTECTION OFPROTEINUSINGTANNINS AND SAPONINS OF RUMEN DIGESTIBILITYAND MICROBESSYNTHESISPROTEIN) Ana Shofi Ani; Retno Iswarin Pujaningsih; Widiyanto .
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (108.859 KB)

Abstract

The objective of this experiment was to examine protection of protein using tannins and saponins toimprove rumen digestibility and microb-mediated protein synthesis in vitro. Rumen fluids used as inoculumwas collected from a composite of two female adult fistulatedongole cattle weighed of ±300 kg with theage of five years old. The experimental design used in this study was a completely randomized design withsix treatments and three replication of each treatment. The six treatments consisted of T0: Proteinconcentrates without protection, T1: protein concentrates protected with 1.2% saponin, T2: proteinconcentrates protected with 0.5% tannin and 0.9% saponin , T3: protein concentrates protected with 1.0%tannin and 0.6% saponin, T4: protein concentrates protected with 1.5 % tannin and 0.3% saponin and T5:protein concentrates protected with 2.0% tannins. The result showed that treatment with tannin, saponinand their combination had a significantly affect (P<0,05) on the level of ammonia (NH3), the total volatilefatty acids (VFA), and total protein. Protection of proteins with combination of 1,0% tannin and 0.6%saponin resulted in best effect on feed protein as shown by its NH3 concentration, total VFA and totalprotein. This indicates the level of protection of feed protein can improve rumen digestibility and microbesmediatedprotein synthesis, as showed in the concentration of N-NH3, total VFA and total protein.
Pengimbuhan Vitamin-E dalam Ransum Kaya Asam Lemak Tidak Jenuh Terhadap Profil Darah Induk Domba Laktasi§ (VITAMIN-E SUPPLEMENTATION ON RICH POLY-UNSATURATED FATTY ACID RATION TO BLOOD PROFILE OF LACTATION EWES) Dilla Mareistia Fassah; Lilis Khotijah
Jurnal Veteriner Vol 17 No 3 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.641 KB)

Abstract

Sunflower oil is a source of rich poly-unsaturated fatty acid (PUFA) mainly Linoleic acid. PUFA on theewes ration was very useful due to its function as the precursors of prostaglandin. It plays an importantrole in the health and reproduction regulation. Early lactation was the critical period where the bodymetabolism is on an increasing stage. However, high level of PUFA on ewe’s lactation ration was also verysusceptible to induce lipid peroxidation which can cause health problems in the affected animals. Thesupplementation of vitamin E as antioxidant is needed to prevent the PUFA from oxidation damage. Theaim of this study was to evaluate the effect of vitamin E supplementation in the rich PUFA diet on theblood profile of lactating ewes. Fifteen ewes on lactation period were used in completely randomizednested design. The level of vitamin E supplementation was 30 IU, 60 IU and 90 IU then the blood profileevaluation was carried out on 28 and 56 days of lactation. The measured variables were haemoglobin,packed cell volume (PCV), erythrocytes, leukocytes, mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC). The number of erythrocytesof ewes was 5.53-9.85 x 106/mm3, the MCV value was 32.19-46.20 fl and the MCH value was 10.91-17.27pg on day-28. The results showed that Vitamin E supplementation has significant effect on the number oferythrocytes (p<0,05), and the high significant effect on MCV as well as MCH values(P<0.01) of lactationewes blood. The Vitamin E supplementation until 90 IU was able to maintain the ewe’s blood profile onthe lactation periods.
Respons Imun Mencit yang Diimunisasi dengan Cysticercus cellulosae (IMMUNE RESPONSE TO TAENIA SOLIUM CYSTICERCOSIS IN MICE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.859 KB)

Abstract

Cysticercosis is a zoonotic parasitic disease which is still problem in Indonesia. The purpose of thisstudy was to investigate immune responses of mice that had been immunized using Taenia solium larval(Cysticercus cellulosae) antigens originated from infected pigs. Three kinds of the C.cellulosaeantigens,secretory and whole antigens were used to immunize three different groups Balb/c mice consisted of 15animals. The serum samples before and after immunization were tested with ELISA test. The resultsshowed that the third antigens induced highly significant titre (P<0,01)compared to unimmunized animals.However no significant different (P>0,05) were found when the third antigens were compared. It wasconcluded that immunization with the three kinds of C. cellulosae antigens can cause immunity in mice.
Avian Influenza Virus H5N1 Remained Exist in Gastrointestinal Tracts of House Flies 24 Hours Post-infection) (VIRUS FLU BURUNG H5N1 TETAP BERADA DALAM SALURAN PENCERNAAN LALAT RUMAH 24 JAM PASCAINFEKSI) Tisna Tyasasmaya; Hastari Wuryastuty; Wasito Wasito; Kai Sievert
Jurnal Veteriner Vol 17 No 2 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

House flies (Musca domestica L.) are one of the major pests found in poultry farms resulting in not onlyannoyance and indirect damage to the poultry production but also transmitting many infectious organismsmechanically and biologically. A highly pathogenic avian influenza virus H5N1 (HPAIV H5N1) collectedfrom field house flies in Java Island, have fully succeeded identified and isolated in 2008. The AIV H5N1isolates were further used in the present study to determine the presence and persistence of the AIV H5N1in the laboratory infected house flies. One hundred house flies from a free AIV poultry farm in Yogyakarta,Indonesia were used in this study. The collected house flies were fasted for 12 hours then divided equallyin control and treated groups. The treated group was allowed to imbibe Dulbecco’s modified eagle medium(DMEM) containing AIV H5N1 while the control group imbibed medium without virus for one hour. Theflies from each group were collected at 12 and 24 hours post-exposure, respectively. All flies were immobilizedat 4°C, immersed in absolute ethanol for a few seconds and dissected under the stereomicroscope to collectthe gastrointestinal (GI) tracts. Based on the RT-PCR results, it is concluded that AIV H5N1 remainedexist in GI tracts of house flies for at least 24 hours post-exposure.
Gambaran Patologi Infeksi Trypanosoma evansi pada Mencit Pascapengobatan dengan Esktrak Ethanol Daun Kipahit (Tithonia diversifolia) (PATHOLOGIC FEATURES OF TRYPANOSOMA EVANSI INFECTION AFTER BEING TREATED WITH ETHANOLIC EXTRACT OF KIPAHIT LEAVES Sutiastuti Wahyuwardani; April H Wardhana; Didik T Subekti; Dharwin Siswantoro
Jurnal Veteriner Vol 19 No 1 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (241.195 KB) | DOI: 10.19087/jveteriner.2018.19.1.11

Abstract

Trypanosoma evansi is a causative agent of Surra disease, which can cause death on infected animals. Surra disease treatment using commercial drugs are expensive, so that it is necessary to look for an alternative drug based on herbal medicine. The present study was carried out to investigate the pathological feature of infected mice after being treated with ethanolic extract of kipahit leaves (Tithonia diversifolia). Forty two male mice weighted 25-30 g of DDY strain were used and divided into seven groups Each group consisted of six mice as replication i.e. uninfected mice as a negative control (P 0), mice infected 104/0.3 mL T. evansi intra peritoneally as a positive control (P 1), uninfected mice treated orally by ethanol extract of kipahit 25 mg/kg BW(P2), infected mice treated orally by ethanol extract of kipahit 25 mg/kg BW then gave booster (P2) on day 6 post infection – double doses (P3), infected mice treated orally by ethanol extract of kipahit 25 mg/kg BW – single dose (P4), infected mice treated by commercial drug – single dose (P5) and infected mice treated by commercial drug – double doses (P6). Anatomy pathological lessions such as splenomegaly, hepatomegaly and pailness of kidney could be identified in mice organs. The histopathologycal lessions such as edema, hemorhhage, infiltration of inflamatory cells and trypanosome parasites in blood vessels could found in liver, kidney, lung, cardiac, spleen tissues of mice, edema also vasculitis found in cerebrum. It was concluded that intraperitoneal administration kipahit ethanol extract at dose 25 mg/BW might potentially reduce the number of T. evansi in the bloodstream of infected animals, but could not prevent and recover from T. evansi inducing organs damage.
Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Daging Ayam pada Pasar Tradisional di Kota Bogor (ANTIBIOTIC RESISTANCE IN ESCHERICHIA COLI ISOLATED FROM CHICKEN MEAT OF TRADITIONAL MARKETS IN THE CITY OF BOGOR) Connie Januari; Mirnawati Bachrum Sudarwanto; Trioso Purnawarman
Jurnal Veteriner Vol 20 No 1 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (151.682 KB) | DOI: 10.19087/jveteriner.2019.20.1.125

Abstract

Antibiotic use in farm is spread widely to treat of poultry disease including therapy, supportive or preventive use and as afeed additive to improve chicken performance. The negative effects of antibiotic use can increase the level of bacterial resistance to antibiotics. This study aimed to investigate on antibiotic resistance in Escherichia coli isolated from chicken meat that were sold in Traditional Market of Bogor City. A total of 175 samples of chicken meat were taken by purposive sampling method, out of 175 found 50 positive samples of E. coli. The samples were subjected to E. coli examination and the isolated E. coli were tested for the antibiotic resistance using eight antibiotics, i.e., amoxicillin, cefotaxime, colistin, nalidixid acid, streptomycin, erythromycin, oxytetracillin, and tetracycline. The study was conducted by using the disk diffusion method on Muller-Hinton agar according to the Clinical and Laboratory Standards Institute guidelines. The study showed E. coli isolated from chicken meat were resistance towards amoxicilin (90%), colistin (94%), nalidixid acid (86%), streptomycin (98%), erythromycin (98%), oxytetracillin (84%), tetracycline (86%), and cefotaxime antibiotics (12%). The proportion of multidrugresistant was 99%. The higher of multidrug-resistant indicated the E. coli would be a threat to public and environmental health.
Behavior of Bali Starling at Bali Barat National Park and Nusa Penida Island (PERILAKU JALAK BALI DI TAMAN NASIONAL BALI BARAT DAN PULAU NUSA PENIDA) Sudaryanto .; Tjut Sugandawaty Djohan; Satyawan Pudyatmoko; Jusup Subagja
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (111.758 KB)

Abstract

Jalak Bali (Leucopsar rothschildi) sejak tahun 1966 dimasukan oleh International Union forConservation of Nature (IUCN) Red List of Threatened Species dan Convention on International Trade inEndangered Species of Wild Fauna and Flora (CITES). Jalak Bali dikelompokan sebagai satwa terancampunah dengan kategori kritis (Critically Endangered) dan di Indonesia telah dilindungi sejak tahun 1970.Tujuan penelitian ini adalah untuk mengetahui perilaku Jalak Bali di Taman Nasional Bali Barat danPulau Nusa Penida untuk konservasi burung tersebut. Metode yang digunakan adalah scan samplingdengan instataneous sampling. Perilaku Jalak Bali di Taman Nasional Bali Barat terdiri dari terbang17%, makan buah 3%, makan serangga 1%, menelisik bulu 15%, menegakkan jambul 6%, bobbing 7%,berkicau 40%, minum 0,5%, dan berjalan 10%. Sementara di Nusa Penida pada musim hujan terdiriterbang 13%, makan buah 19%, makan serangga 4%, menelisik bulu 7%, menegakkan jambul 7%, bobbing8%, berkicau 11%, minum 1%, 1% mandi, berjalan 16% , bersarang 2%, mengeram 9% dan mengasuh anak3%, dan di Nusa Penida pada musim kemarau terdiri terbang 11%, makan buah 9%, makan serangga21%, menelisik bulu 7%, menegakkan jambul 6%, bobbing 7%, berkicau 9%, minum 2%, mandi 1%, berjalan18% , dan bersarang 8%. Jalak Bali berkembang biak di Nusa Penida pada musim hujan dan musimkemarau, tetapi di Taman Nasional Bali Barat pada musim hujan.

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