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Padjadjaran Journal of Dentistry

PJD
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Published by Universitas Padjadjaran

ISSN : 19790201 EISSN : 25496212 DOI : -

Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Public Health

Padjadjaran Journal of Dentistry or known as PJD, is an English-language scientific periodicals published by the Faculty of Dentistry Universitas Padjadjaran thrice a year on every March, July and November. The submission process of manuscript is open throughout the year. All submitted manuscripts will go through the double-blind peer review and editorial review before being granted with acceptance for publication. Designed as a medium of information and scientific knowledge, Padjadjaran Journal of Dentistry publishes numerous research articles, in the area of Oral Biology, Dental Material Science and Technology, Oral and Maxillofacial Surgery, Pedodontics, Dental Public Health and Community Dentistry, Conservative Dentistry, Periodontics, Prosthodontics, Orthodontics, Oral Medicine, Dental Radiology, as well as with their development through interdisciplinary and multidisciplinary approach. Initially published as Padjadjaran Journal of Dentistry (PJD) magazine ISSN 1979-0201(print) on 2007 in form of printed issues. In 2017, the magazine had its online published version under the same with ISSN 2549-6212 (online) in accordance with the policy of LIPI. Padjadjaran Journal of Dentistry has been using Open Journal System requiring all writers to register in advance before they are allowed to upload the manuscript they write online. Afterwards, the editors, peer reviewers, and writers can monitor the manuscript processing. Several other changes are informed in the Journal History.

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Search results for , issue "Vol 31, No 1 (2019): March 2019" : 12 Documents clear

Serum IL-6 levels of atopic patients with recurrent aphthous stomatitis (RAS) Nur'aeny, Nanan; Sufiawati, Irna; Suwarsa, Oki; Gurnida, Dida Akhmad
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Atopy is a genetic tendency of allergic condition with specific clinical manifestations such as atopic dermatitis (AD), allergic rhinitis (AR), and bronchial asthma (BA). Atopic individuals might experience other health problems that coincide with atopic manifestations, including the appearance of recurrent aphthous stomatitis (RAS) which is an oral mucosal disease that can also be influenced by allergies. This study was aimed to be the preliminary study regarding the level of IL-6 serum in atopic patients who were also having RAS. Methods: Thirty subjects were recruited and grouped into three groups with ten subjects each were group of atopic (dermatitis, allergic rhinitis, or bronchial asthma) with RAS, atopic without RAS, and control. Blood specimens were obtained with an approved institutional board review protocol. This research was cross-sectional research with consecutive sampling method conducted at Dr Hasan Sadikin Hospital Bandung. Results: There were significant differences in IL-6 log levels in all three groups (p < 0.001). The higher IL-6 levels in the group of atopic with RAS might be due to the influence of atopy because this condition can increase the acute inflammation of RAS. IL-6 is a pro-inflammatory marker found in the circulation of atopic and RAS patients. Conclusion: Serum IL-6 levels in the group of atopic patients with RAS is higher than in other groups. Keywords: Serum, IL-6, atopic, recurrent aphthous stomatitis

Differences in head size and shape during the growth of Deutero Malay children Primasari, Ameta; Angelia, Veronica; Agara, Dimas
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: The growth periods of 0-18 years old can be studied with anthropometry by identifying the head size and shape of the human head. Study on vertical, transverse, and horizontal cephalic indexes can understand differences and growth between sexes and age groups. This study was aimed to analyse the averages and differences in the head size and shape of Deutero Malay children in different age groups. Methods: This research was a descriptive analytic study with cross-sectional design. The sample of 192 Deutero Malay children consisted of boys and girls with an age range of 7-18 years old. Measurement of head height, length, and width was performed to calculate the differences of vertical, transverse, and horizontal cephalic index values by sex and age group, and the classification of the head shape based on the cephalic index values. Results: There was a difference of vertical, transverse, and horizontal by age group, with p = 0.000 (p < 0.05). The average of vertical, transverse, and horizontal cephalic index values was significantly higher in each age group. The average horizontal cephalic index was significantly higher in boys and girls. There was no significant difference in the head shape by the vertical and horizontal cephalic index in all age groups. The highest vertical cephalic index found was chamaecephalic (68%). The highest transverse cephalic index was tapeiocephalic (99%). The highest horizontal cephalic index was hyperbrachycephalic (80%). Conclusion: There is similar head shape of Deutero Malay children, although there may be increasing head size along with age. Keywords: Deutero Malay, head shape, cephalic index, transverse cephalic index, horizontal cephalic index, vertical cephalic index

Effect of cassava (Manihot esculenta cranzt), rice (Oryza sativa L.), and potato (Solanum tuberosum) water extract to decrease pH phase fermentation of Streptococcus mutans ATCC 25175 Imelda, Reza Afri; Mariam, Marry Siti; Satari, Mieke Hemiawati
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Dental caries is the localised damage of the hard tooth tissue caused by acid production results of carbohydrate fermentation by acid-producing bacteria. Streptococcus mutans are one of the aetiological bacteria which fermented carbohydrate causes a decrease in the oral pH. Carbohydrate is generally consumed, included cassava, rice, and potato. The purpose of this study was to determine the mechanism of the water extract of rice, cassava, and potato in decreasing the pH of Streptococcus mutans culture to know their potential in causing caries and also determined decreasing differences between three water extracts. Methods: The experimental method was used in cultured Streptococcus mutans ATCC 25175 and tested against the treated rice, cassava, and potato water extract. Cultured bacteria were incubated for 48 hours in facultative anaerobes then measured the pH with a digital pH meter. The measurement result was statistically tested with the paired t-test and ANOVA. Results: The paired t-test (α = 0.05) showed that the p-value of rice, cassava, and potato water extract were 0.001, 0.001, and 0.018 respectively. ANOVA test with an α value of 0.05 showed the p-value of 0.000. Conclusion: There was a decrease in pH of cultured Streptococcus mutans after administration of the water extract of cassava, rice, and potato, and there was a difference between the pH decrease of the Streptococcus mutans between administration of water extract of rice, cassava, and potato. Keywords: Streptococcus mutans, Manihot esculentacrantz, Oryza sativa L., Solanum tuberosum, pH phase fermentation

Velocity and wall shear stress of 18% EDTA irrigation solution flow in the removal of Ca(OH)2 with computational fluid dynamic analysis Muryani, Anna; Dharsono, Hendra Dian Adhita; Zuleika, Zuleika; Moelyadi, Mochammad Agoes; Fatriadi, Fajar
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Ethylene Diamine Tetra Acetic Acid (EDTA) 18% irrigation solution is one of the chelating agents that able to remove calcium hydroxide (Ca(OH)2) from the root canal wall. Failure of root canal treatment can be caused by the presence of residual calcium hydroxide in the root canal wall, thus blocking the bond between the medication and root canal filling material with the root canal wall. This study was aimed to observe the EDTA 18% flow in removing the Ca(OH)2 using CFD analysis. Methods: This research was descriptive. Cleanliness of the root canal wall from Ca(OH)2 medicament using EDTA 18% irrigation was analysed using the CFD method with test specimens in the form of resin blocks made according to the characteristics of the root canal. The irrigation needle used was side-vented with a position of 3 mm from the apical tooth. Stage analysis of root canal geometry was performed using Computational Fluid Dynamics (CFD) analysis to observe the characteristics of irrigation solutions in root canals in 3D. Results: The streamlined characteristics of EDTA 18% irrigation solution showed a unique behaviour due to the features of the side-vent shaped irrigation needle. Irrigation flow in the crown area of the inlet (side-vented irrigation needle) showed low velocity so that the fluid flow when exiting the inlet was more towards the apical than the outlet (root canal orifice). Conclusion: Velocity and wall shear stress of EDTA 18% showed the results validation conformity between experimental and CFD, that the maximum velocity of EDTA 18% is 19 ms-1 and EDTA 18% wall shear stress is 1.56 KPa for calcium hydroxide removal observed from the CFD study. Keywords: Computational fluid dynamics, EDTA 18%, calcium hydroxide (Ca(OH)2), velocity, wall shear stress

Docetaxel hydrate induces apoptosis and suppresses tumorigenesis of oral Burkitt’s lymphoma cells (in vitro and in vivo studies) Supriatno, Supriatno
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Burkitt’s lymphoma (BL) is one of the tumours with high malignancy and rapid cell growth, derived from B-cell lymphoma. BL typically found in children at dengue-endemic and HIV-AIDS areas with low socioeconomic levels. This study was aimed to analyse the induction of apoptosis and the suppression of tumorigenesis of oral Burkitt’s lymphoma (Raji) cells using docetaxel hydrate in vitro and in vivo. Methods: In the present study, the pure experimental laboratory with post-test only control group design was carried out. Raji cell cultures were incubated with docetaxel hydrate by doses of 0, 1.25 x 10-2, 2.5 x 10-2, and 5.0 x 10-2 M; and IC50 carboplatin (3.1 x 10-6 M) as a positive control. Induction of apoptotic was analysed by double staining of acridine orange-ethidium bromide. Tumorigenesis assay was performed by inoculating Raji cells in nude mice flanks at 1 x 106 cells/mice. Tumour treatment was delivered by various doses of docetaxel hydrate peroral. Results: Apoptosis cells were significantly increased in Raji cells treated with docetaxel hydrate by doses of 2.5 x 10-2 and 5.0 x 10-2 M. The tumour volume in mice given doses of 2.5 x 10-2 and 5.0 x 10-2 M was markedly decreasing compared to control (dose of 0). Conclusion: Docetaxel hydrate has a high antitumour potency by inhibiting tumorigenesis and increasing apoptosis of Burkitt’s lymphoma cells. Keywords: Docetaxel hydrate, double staining, Burkitt’s lymphoma cell, apoptosis, tumorigenesis

Changes in soft tissue facial profile of class II skeletal malocclusion patients with retrognathic mandible treated with twin block appliance Lin, Ng Hui; Soemantri, Eky Setiawan Soeria; Gayatri, Gita
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: The soft tissue aspect in orthodontics treatment has gained attention in the last few years. The soft tissue profile is said to reflect the underlying skeletal profile, which causes a convex profile in patients with class II skeletal malocclusion. This research was aimed to determine the changes in the soft tissue facial profile of class II skeletal malocclusion patients with retrognathic mandible after twin block treatment. Methods: The type of research used in this study was retrospective descriptive research with paired t-test. The population was children aged 10-13 years old with class II skeletal malocclusion that were treated with twin block appliance in the Faculty of Dentistry Universitas Padjadjaran, Indonesia. The results of soft tissue changes before and after twin block treatment were compared. Results: There was an insignificant increase in soft tissue profile angle and Holdaway’s soft tissue angle after twin block treatment (p > 0.05). Whereas, Holdaway’s H-angle was decreasing and Merrifield’s Z-angle was increasing after twin block treatment, with statistically significant difference (p < 0.05). Conclusion: There was a decrease of H-angle, indicates a reduction in facial convexity and improvement of the facial profile after twin block treatment, but no difference in soft tissue profile angle and Holdaway’s soft tissue angle after twin block treatment. Keywords: Facial soft tissue profile, class II skeletal malocclusion, retrognathic mandible, twin block appliance

Diagnosis determination of chronic periapical abscess case using imaging radiography software and microbiological examination in female patients Prisinda, Diani; Malinda, Yuti; Lita, Yurika Ambar; Tjahajawati, Sri
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Diagnosis determination of chronic periapical abscess can be achieved by subjective, objective, and other examination such as radiographic and microbiological examination based on clinical finding and differences intensity at the periapical area with healthy bone suspected to be chronic abscess periapical. A microbiological examination performed to obtain the predominant bacteria that involved to determine an adequate medicament to succeed endodontic treatment. This study was aimed to compare intensity abscess lesion segmentation image reconstruction and surrounding bone for determine the diagnosis of periapical abscess more accurately and to determine the characteristic of bacteria colony from a periapical abscess tooth. Methods: The study sampling method was consecutive sampling (6 female) consisted of inclusion criteria patient with a periapical lesion in anterior maxillary that diagnosed based on clinical signs and symptoms. CBCT 3D scan of the maxillofacial region taken from of patients was measured image intensity periapical lesion and surrounding bone using semi-auto segmentation with ITK-SNAP 3.6.0. Samples from the root canals taken using three sequential sterile paper points. The samples were plated, and microorganisms were then isolated and identiﬁed by the colony characteristics. Results: There was a signiﬁcant difference between the mean content of lesion intensity of (n = 6,256.710 ± 81.930 and 636.022 ± 79.981 lesion boundary. The two-tailed p-value = 0.0050, this difference was considered to be very statistically significant. The mean of group lesion minus group surrounding bone equals to 163.715. 95% confidence interval of this difference from 75.542 to 251.888. Conclusions: Demineralisation of periapical bone can be determined by voxel intensity value in CBCT 3D radiograph using semi-auto segmentation ITK SNAP, could be recommended as a quantitative interpretation for detection periapical lesion and chronic periapical abscesses is a poly-microbial disease. Keywords: Chronic periapical abscess, endodontic, microbiology, radiograph CBCT

Differences of inter-canine distance on dental cast model, wax impression tracing, radiograph of dental impression tracing, and dental cast tracing methods of human bite mark Fidya, Fidya; Swastirani, Astika
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Faculty of Dentistry Universitas Padjadjaran

Introduction: Bite marks are unique to each, differing between one person and another. Several methods to analyse bite mark have been developed during certain periods. However, a standard method to analyse and evaluate the bite mark anatomic variations has not been developed. The objective of this research was to identify the differences of the inter-canine distance on dental cast model, wax impression tracing, radiograph of dental impression tracing, and dental cast tracing methods of the human bite mark. Methods: The subject of the research was as much as 30 consecutive dental cast model, wax impression tracing, radiograph of dental impression tracing, and dental cast tracing for both maxilla and mandible. Measurements performed on each group were conducted in the inter-canine areas using a Vernier calliper. The gold standard that used as a comparator was a dental cast model group on both maxilla and mandible. Comparison of the measurement results was carried out and analysed using a one-way ANOVA test and Tukey LSD test. Results: The average sizes of maxilla and mandible were 37.93 mm and 31.70 mm for dental cast model; 37.93 mm and 32.83 for wax impression tracing; 38.20 mm and 31.70 mm for radiograph dental impression tracing; and 36.65 mm and 30.76 mm of dental cast tracing. The significance value of the maxilla was p = 0.008 (p < 0.05), and the significance value of mandible was p = 0.000 (p < 0.05). Conclusion: The inter-canine distance of radiograph tracing group is found to be most similar with dental cast group as gold standard both in the maxilla and the mandible. Keywords: Bite mark, dental cast, wax impression, radiograph, tracing

β-Carotene gingival mucoadhesive patch on Cx-43 and 8-Oxo-dg expression to prevent micronucleus formation due to panoramic radiography exposure Shantiningsih, Rurie Ratna; Suwaldi, Suwaldi; Astuti, Indwiani; Mudjosemedi, Munakhir
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: β-carotene as a lipid-soluble antioxidant substance, expected to prevents the oxidative reaction and to improve the junctional communication detected by Cx-43. Micronucleus is known as one of the early markers of DNA damage which might be related to carcinogenesis mechanism that increased by panoramic radiography radiation exposure. The objective of this research was to analyse the effect of the β-carotene gingival mucoadhesive patch on Cx-43 expression and 8-oxo-dG to prevent micronucleus formation at the gingival mucosa of New Zealand rabbit due to panoramic radiography radiation exposure. Methods: β-carotene gingival mucoadhesive patch was applied to gingival mucosa of the experimental rabbits. In Group I, the rabbits without patch was a control group. Group II has applied the patch before radiography exposures, Group III during the radiography exposures, and Group IV after radiography exposures. Micronucleus samples were taken from the rabbit’s swabbed gingival mucosa, then stained using modified Feulgen-Rossenbeck. The 8-oxo-dG and Cx-43 expressions were detected using immunohistochemistry technique. The results were then analysed using two-way ANOVA. Results: The increasing number of micronucleus in Group I had a different mean (p < 0.05) with Group III and IV. The expression of 8-oxo-dG and Cx-43 had significant differences (p < 0.05) between Group I and III. This results indicated that β-carotene gingival mucoadhesive patch prevents the increasing number of micronucleus especially when it applied before and during panoramic radiography radiation exposure by the increasing Cx-43 and decreasing 8-oxo-dG expression. Conclusion: The β-carotene gingival mucoadhesive patch can be utilised to prevent the panoramic radiography radiation exposure’s effect. Keywords: β-carotene, gingival-mucoadhesive patch, micronucleus, radiation exposure

Effectiveness of Sargassum sp. ethanolic extract on traumatic ulcers healing in the labial mucosa of Wistar strain (Rattus norvegicus) Arwidasari, Annisya Ristie; Cevanti, Twi Agnita; Soewondo, Isidora Karsini
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Universitas Padjadjaran

Introduction: Oral ulcer lesions are one of the most common lesions in the oral cavity. The prevalence of traumatic ulcers is quite high compared to other oral lesions. Brown algae, Sargassum sp. is one of the algae contains flavonoids, vitamin A, vitamin C, iron, and calcium, which play a role in wound healing and has economic value. This study was aimed to determine the effectiveness of Sargassum sp. ethanolic extract on traumatic ulcers healing in the labial mucosa of Wistar strain (Rattus norvegicus). Methods: 28 Wistar strain (Rattus norvegicus) weighed 150-200 grams. The samples were divided into 4 groups (K1, K2, P1, P2); each group consisted of 7 mice. K1 group was administered with Hydroxy Propyl Methyl Cellulose (HPMC), K2 was administered with 0.2% hyaluronic acid gel, P1 was administered with 50% Sargassum sp. extract gel, and P2 was administered with 75% Sargassum sp. extract gel. In each group, the ulcer was made on the mice labial mucosa; then the ulcer diameter was measured and observed on day 1 and day 7. Results: The mean diameter ± standard deviation of traumatic ulcer calculations were K1 (1.09 ± 0.40), K2 (1.81 ± 0.67), P1 (1.39 ± 0.43) and P2 (2.16 ± 0.49) respectively. One way ANOVA test results showed significant differences in K1, K2, P1, and P2 groups. Conclusion: Ethanol extract of Sargassum sp. is effective in accelerating the traumatic ulcers healing at the concentration of 75%.Keywords: Traumatic ulcer, Sargassum sp., wound healing.

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