Article Per Year (5 Year)
p-Index From 2020 - 2025
1.704
P-Index
This Author published in this journals
All Journal Indonesian Journal of Applied Sciences Majalah Kedokteran Bandung Jurnal Kesehatan Masyarakat Althea Medical Journal Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Journal of Mathematical and Fundamental Sciences Berkala Ilmu Kesehatan Kulit dan Kelamin Jurnal Farmasi Klinik Indonesia Indonesian Journal of Tropical and Infectious Disease Padjadjaran Journal of Dentistry Journal Of Vocational Health Studies Jurnal Kesehatan Prima Jurnal Pendidikan dan Pelayanan Kebidanan Indonesia (Indonesian Journal of Education and Midwifery Care) Majalah Kedokteran Andalas Jurnal Aisyah : Jurnal Ilmu Kesehatan Jurnal Integrasi Kesehatan dan Sains Jurnal Ilmiah JKA (Jurnal Kesehatan Aeromedika) Jurnal Ilmu Kebidanan dan Kesehatan Media Dermato-Venereologica Indonesiana
Oki Suwarsa
Departemen Ilmu Kesehatan Kulit Dan Kelamin Universitas Padjadjaran/ RS. Dr. Hasan Sadikin Bandung
Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Physics Public Health Veterinary

Published : 34 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 34 Documents
Search

 1   2   3   4 
PENGARUH MEDIA FILM TERHADAP SIKAP IBU PADA DETEKSI DINI KANKER SERVIKS Mulyati, Sri; Suwarsa, Oki; Desy Arya, Insi Farisa
Jurnal Kesehatan Masyarakat Vol 11, No 1 (2015): JURNAL KESEHATAN MASYARAKAT (KEMAS) JULI 2015
Publisher : Jurusan Ilmu Kesehatan Masyarakat Fakultas Ilmu Keolahragaan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v11i1.3401

Abstract

Salah satu cara yang praktis dan murah untuk mencegah terjadinya kanker serviks adalah melalui tes Inspeksi Visual Asam Asetat (IVA). Upaya untuk mempengaruhi sikap ibu pada tes IVA adalah pendidikan kesehatan melalui film. Tujuan penelitian ini adalah untuk mengetahui pengaruh pendidikan kesehatan melalui film terhadap sikap ibu pada tes IVA. Penelitian ini dilakukan pada tahun 2014. Rancangan penelitian ini adalah non randomized within group design. Pengambilan sampel dilakukan dengan teknik consecutive sampling pada 60 responden.  Analisis bivariat yang digunakan pada penelitian ini adalah  uji wilcoxon. Hasil penelitian menunjukkan terdapat pengaruh positif pendidikan kesehatan melalui film terhadap sikap ibu (P<0,05) dengan peningkatan median (rentang) skor sikap dari 44,23 (19,23-75) menjadi 78,85 (25-94,23). Kesimpulan penelitian ini adalah terdapat pengaruh positif pendidikan kesehatan melalui film terhadap sikap ibu. The Visual Inspection with Acetic Acid (VIA) test is a practical and cheaper way to prevent cervical cancer. One way to influence attitude in VIA test is health education based on the movie. The purpose of this study was to determine the effect of health education based on the movie on the attitude,of women in VIA test. The study conducted at 2014. The study design was nonrandomized within group design. The sampling technique was consecutive sampling technique on 60 respondents. The data analysis employed  bivariable analysis with wilcoxon test. This study shows there was a significant positive effect of the health education based on the movie on the mother’s attitude (P<0.05) with median and range attitude score increasing from 44.23 (19.23-75) to 78.85(25-94.23). This study concludes that there is significant positive effect of the health education based on the movie on attitude.
Quality of Life in Children with Atopic Dermatitis Wicaksana, Muhammad Akbar; Suwarsa, Oki; Dwiyatnaningrum, Fenny
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (841.296 KB) | DOI: 10.15850/amj.v4n3.652

Abstract

Background: Atopic dermatitis (AD) is the most common chronic skin disease in children which caused significant morbidity and impaired quality of life (QoL). The main goal of AD therapy is to prevent flare -ups, prolong remission and increase QoL. Therefore, the study aimed to discover QoL in children with AD.Methods: The study was conducted at Pasundan Public Health Centre, Al Islam General Hospital and Kimia Farma Private Dermatology Clinic, from September to November 2015. This descriptive study used consecutive sampling with 24 outpatients who were admitted to the health facility and diagnosed as AD. A questionnaire on Infant Dermatitis Quality of Life for infants aged 0–4 years, and Children Dermatology Life Quality Index for children aged 5–16 years was used in this study to measure QoL.Results: Out of 24 patients, 9 patients aged 0–4 years had mean score of 4.44±4.36, and 15 patients aged 5–16 years had mean score of 5.80±3.95. Mean SCORAD Objective in patients aged 0–4 and 5–16 was 15.61±7.75 and 17.44±11.Conclusions: The QoL in children with AD vary among patients. Most of the patients have mild-moderate impairment in QoL. 
Relationship between Knowledge, Attitude and Practice of Student’s Personal Hygiene with Scabies Incidence in Pesantren Darul Fatwa, Jatinangor, West Java, Indonesia Purnama, Karinna Dwi; Sjambas, Dedi Rachmadi; Suwarsa, Oki
Althea Medical Journal Vol 4, No 3 (2017)
Publisher : Althea Medical Journal

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (621.223 KB) | DOI: 10.15850/amj.v4n3.1198

Abstract

Background: Scabies is a parasitic infection caused by Sarcoptesscabiei Hominis varian and Acarina order. Sarcoptes scabiei infects both male and female in any age groups, ethnics, and socio-economic levels. Transmission of scabies can pass through by a direct contact from skin-to-skin or indirect contact through sharing bed, clothes, and towels. Pesantren as an educational institution supplies facilities that are shared thus making students susceptible to scabies infection. This study was conducted to discoverrelationshipbetween knowledge, attitude, and practice of student’s personal hygiene with scabies incidence.Methods: An analytic observational study was conducted from October to November 2015 in Pesantren Darul Fatwa, Jatinangor. Study was conducted with cross-sectional design and total sampling for the sample size. Total of 37 students whoparticipated in this study were 22 malesand 15 females. This study was begun with a stand alone-questionnaire, fill out on knowledge, attitude, and practice of personal hygiene that was supervised by researcher, and physical examination for scabies diagnosis that was performed by doctors.Results: Based on history taking and physical examination, 15 out of 37 students were diagnosed with scabies positive. All of them were males, dominated by 13 year old students, 1st and 2nd Junior High School students. Statistical analysis with chi-square test showed that there were no relationship between knowledge, attitude and practice of student’s personal hygiene with  scabies incidence.Conclusions: There is no relationship between knowledge, attitude and practice of student’s personal hygiene with  scabies incidence in Pesantren
PENGARUH MEDIA FILM TERHADAP SIKAP IBU PADA DETEKSI DINI KANKER SERVIKS Mulyati, Sri; Suwarsa, Oki; Desy Arya, Insi Farisa
KEMAS: Jurnal Kesehatan Masyarakat Vol 11, No 1 (2015)
Publisher : Department of Public Health, Faculty of Sport Science, Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/kemas.v11i1.3401

Abstract

Salah satu cara yang praktis dan murah untuk mencegah terjadinya kanker serviks adalah melalui tes Inspeksi Visual Asam Asetat (IVA). Upaya untuk mempengaruhi sikap ibu pada tes IVA adalah pendidikan kesehatan melalui film. Tujuan penelitian ini adalah untuk mengetahui pengaruh pendidikan kesehatan melalui film terhadap sikap ibu pada tes IVA. Penelitian ini dilakukan pada tahun 2014. Rancangan penelitian ini adalah non randomized within group design. Pengambilan sampel dilakukan dengan teknik consecutive sampling pada 60 responden.  Analisis bivariat yang digunakan pada penelitian ini adalah  uji wilcoxon. Hasil penelitian menunjukkan terdapat pengaruh positif pendidikan kesehatan melalui film terhadap sikap ibu (P<0,05) dengan peningkatan median (rentang) skor sikap dari 44,23 (19,23-75) menjadi 78,85 (25-94,23). Kesimpulan penelitian ini adalah terdapat pengaruh positif pendidikan kesehatan melalui film terhadap sikap ibu. The Visual Inspection with Acetic Acid (VIA) test is a practical and cheaper way to prevent cervical cancer. One way to influence attitude in VIA test is health education based on the movie. The purpose of this study was to determine the effect of health education based on the movie on the attitude,of women in VIA test. The study conducted at 2014. The study design was nonrandomized within group design. The sampling technique was consecutive sampling technique on 60 respondents. The data analysis employed  bivariable analysis with wilcoxon test. This study shows there was a significant positive effect of the health education based on the movie on the mother’s attitude (P<0.05) with median and range attitude score increasing from 44.23 (19.23-75) to 78.85(25-94.23). This study concludes that there is significant positive effect of the health education based on the movie on attitude.
PERBANDINGAN KADAR INTERLEUKIN-17 SERUM PASIEN AKNE VULGARIS TIPE PAPULOPUSTULAR DENGAN KOMEDONAL Maulinda, Shinta; Hindritiani, Reti; Ruchiatan, Kartika; Suwarsa, Oki
Majalah Kedokteran Bandung Vol 48, No 3 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8.208 KB) | DOI: 10.15395/mkb.v48n3.846

Abstract

Patogenesis akne vulgaris (AV) bersifat multifaktorial dan faktor yang berperan penting adalah inflamasi yang terutama diinduksi oleh reaksi imunologis terhadap Propionibacterium acnes (P. acnes). Bakteri ini dapat ditemukan baik pada lesi inflamasi berupa papula dan pustula, maupun noninflamasi seperti komedo, dengan jumlah P. acnes lebih tinggi pada lesi inflamasi. Secara klinis komedo merupakan lesi noninflamasi, namun secara mikroskopis sudah terjadi inflamasi. P. acnes dapat menginduksi pelepasan sitokin proinflamasi antara lain IL-17. Tujuan penelitian ini adalah membandingkan kadar IL-17 serum antara pasien AV tipe papulopustular dan komedonal. Penelitian ini merupakan penelitian analitik observasional secara potong lintang dengan peserta penelitian masing-masing 12 pasien AV tipe papulopustular dan komedonal derajat dua atau lebih di Poliklinik Dermatologi Kosmetik Departemen Ilmu Kesehatan Kulit dan Kelamin Rumah Sakit Dr. Hasan Sadikin Bandung selama periode Oktober?November 2014. Pada kedua kelompok dilakukan pengambilan darah tepi untuk pengukuran kadar IL-17 serum dengan metode ELISA. Hasil penelitian menunjukkan kadar IL-17 serum rata-rata (SD) pada pasien AV tipe papulopustular adalah 0,65 (1,12) pg/mL, sedangkan pada tipe komedonal adalah 0,46 (0,42) pg/mL, perbedaan tersebut secara statistik tidak bermakna (p=1,000). Simpulan, kadar IL-17 serum pasien AV tipe papulopustular tidak berbeda dibanding dengan AV tipe komedonal menunjukkan bahwa kemungkinan pada lesi komedo sudah terjadi inflamasi. [MKB. 2016;48(3):160?3]Kata kunci: Akne vulgaris, IL-17 serum, tipe komedonal, tipe papulopustular Comparison of Interleukin-17 Serum Level between Papulopustular and Comedonal Types of Acne VulgarisThe pathogenesis of acne vulgaris (AV) is multifactorial and inflammation, which is primarily cause by induction of immunological response to Propionibacterium acnes (P. acnes), is suggested as the main important factor in AV development. These bacteria can be found in the form of papules and pustules in inflammatory lesions and as comedones in noninflammatory lesions, with a higher presentation seen in the inflammatory lesions. Clinically, comedo is a noninflammatory lesion; however, inflammation can be observed microscopically. P. acnes can release proinflammatory cytokines, including IL-17. The aim of this study was to compare the IL-17 serum level between papulopustular type and comedonal type of AV. This study was a cross sectional-analytic observational, conducted at the Cosmetic Dermatology Clinic, Dermatology and Venereology, Dr. Hasan Sadikin General Hospital Bandung during October?November 2014. The participants were AV patients with at least second degree papulopustular (n12) and comedonal types (n12). Blood samples from all subjects were collected and the IL-17 serum levels were measured using ELISA methods. This study showed that the mean (single deviation) IL-17 serum levels in papulopustular and comedonal type of AV were 0.65 (1.12) pg/mL and 0.46 (0.42) pg/mL, respectively, and the difference was not statistically significant (p:1.000). The IL-17 serum level in the papulopustular type is not different from that of the comedonal type of AV in this study, which indicates that the inflammatory events probably had occurred  in comedonal lesions. [MKB. 2016;48(3):160?3]Key words: Acne vulgaris, comedonal type, IL-17 serum level, papulopustular type
Kadar Interleukin-18 pada Kultur Limfosit Penderita Dermatitis Atopik yang Distimulasi Staphylococcal Enterotoxin B (SEB) Suwarsa, Oki; Sudigdoadi, -; Sutedja, Endang; Idjradinata, Ponpon
Majalah Kedokteran Bandung Vol 47, No 4 (2015)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (256.751 KB)

Abstract

Staphylococcus aureus (S. aureus) mempunyai peranan penting pada patogenesis dermatitis atopik (DA). Peran S. aureus tersebut tidak hanya sebagai pencetus DA, tetapi juga menyebabkan inflamasi kronik. Peran tersebut berhubungan dengan dihasilkannya protein antara lain toksin poten oleh S. aureus, yaitu Staphylococcal enterotoxin B (SEB). Interleukin-18 (IL-18) merupakan regulator penting dari produksi sitokin Th-1 yaitu interferon-γ (IFN-γ).  Tujuan penelitian ini adalah mengetahui kadar IL-18 pada kultur limfosit pasien DA yang distimulus dengan SEB. Penelitian ini dilakukan pada 20 orang penderita DA (7 laki-laki dan 13 perempuan) dan 20 orang sehat (9 laki-laki dan 11 perempuan) di Rumah Sakit Dr. Hasan Sadikin Bandung, merupakan penelitian eksperimental secara in vitro pada kultur ilmfosit yang distimulus dengan SEB di Laboratorium Penelitian dan Pengujian Terpadu Universitas Gadjah Mada. Terjadi peningkatan kadar IL-18 rata-rata pada kultur limfosit antara sebelum dipapar dan setelah dipapar SEB, baik pada kelompok DA maupun kelompok kontrol. Setelah dilakukan uji statistik perbandingan antara kadar IL-18 rata-rata sebelum dan sesudah dipapar SEB antara kelompok DA dan kontrol, didapatkan hasil kadar IL-18 kelompok DA lebih tinggi bermakna dibanding  dengan kelompok kontrol (p<0,05) sehingga dapat disimpulkan kadar IL-18 meningkat tinggi pada kelompok DA yang dipapar SEB. [MKB. 2015;47(4):249–54]Kata kunci: Dermatitis atopik, interleukin-18 (IL-18), Staphylococcus enterotoxin BInterleukin-18 Levels in Lymphocytes Cultures from Atopic Dermatitis Patients Stimulated by Staphylococcal Enterotoxin BAbstractStaphylococcus aureus (S. aureus) has an important role in the pathogenesis of atopic dermatitis (AD). S. aureus acts as a triggering factor for AD and also causes chronic inflammation. These roles of S. aureus are related to various proteins such as Staphylococcal enterotoxin B (SEB) as a potent toxin. Interleukin-18 (IL-18) is an important regulator of cytokine production of Th-1, which is interferon-γ (IFN-γ). The aim of this study was to reveal the levels of IL-18 in cultured lymphocytes from AD patients exposed by SEB. This study was conducted on 20 people with DA (7 men and 13 women) and 20 healthy volunteers (9 men and 11 women) in dr. Hasan Sadikin General Hospital. The in vitro experimental study on cultured lympocytes exposed with SEB was performed at the Integrated Research and Testing Laboratory of Gadjah Mada University. The average levels of IL-18 in cultured lymphocytes before and after being exposed to SEB increased both in AD group and control group. After the statistical tests was performed on the ratio of the average levels of IL-18 before and after being exposed to SEB between AD and control groups, it was shown that the levels of IL-18 AD group was significantly higher than the control group (p <0.05). Therefore, it can be concluded that the levels of IL-18 increased higher in AD group exposed by SEB. [MKB. 2015;47(4):249–54]Key words: Atopic dermatitis , interleukin-18, Staphylococcal enterotoxin BDOI: 10.15395/mkb.v47n4.620
Keberhasilan Terapi Kombinasi Menggunakan Metotreksat Injeksi dan Siklosporin pada Psoriasis Pustulosa Generalisata Suwarsa, Oki; Devi Nursjamsi, Nadilla Carissa; Pangastuti, Miranti; Sutedja, Endang; Dharmadji, Hartati Purbo
Majalah Kedokteran Bandung Vol 49, No 3 (2017)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1846.617 KB) | DOI: 10.15395/mkb.v49n3.1123

Abstract

Psoriasis pustulosa generalisata (PPG) adalah bentuk khusus dari psoriasis yang bersifat akut dan berat. Etiologi yang belum diketahui secara pasti menyebabkan pengobatan PPG masih menjadi suatu tantangan. Terapi kombinasi dapat diberikan pada PPG yang berat maupun yang tidak memberikan respons terhadap terapi sistemik tunggal, selain itu terapi kombinasi juga dapat meningkatkan efektivitas dari obat tunggal. Dilaporkan satu kasus PPG pada seorang wanita yang diterapi dengan menggunakan kombinasi metotreksat (MTX)  injeksi dan siklosporin. Dari anamnesis dan pemeriksaan fisis didapatkan pustula berkelompok pada dasar makula eritem yang bergabung membentuk lake of pus, disertai gejala sistemik demam dan leukositosis. Keluhan tersebut sudah berulang sejak 13 tahun yang lalu. Pasien diberikan terapi kombinasi injeksi MTX dan siklosporin. Pustula pada pasien menghilang setelah tujuh hari pemberian obat. Terapi kombinasi dengan MTX injeksi dan siklosporin ini baru pertama kali dilakukan 19 Mei–26 Mei 2017 di Departemen Ilmu Kesehatan Kulit dan Kelamin Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Umum Pusat Dr. Hasan Sadikin Bandung dan memberikan hasil yang baik. [MKB. 2017;49(3):208–12]Kata kunci: Metotreksat, metotreksat injeksi, psoriasis pustulosa generalisata, siklosporin Succesful Treatment of Generalized Pustular Psoriasis with a Combination of Methotrexate Injection and CyclosporineGeneralized pustular psoriasis (GPP) is a special form of acute and severe psoriasis. The definite etiology of GPP remains a challenge. Combination therapy may be given to severe GPP or GPP that does not respond to a single systemic therapy sincecombination therapy may also improve the effectiveness of a monotherapy drug. A GPP case was reported in a woman treated with a combination of methotrexate (MTX) and cyclosporine. From anamnesis and physical examination it was revealed that there were clustered  pustules on the base of erythematous macules that form a lake of pus accompanied by systemic symptoms of fever and leukocytosis. This was a recurrent complaint since 13 years ago. Patients were given combination therapy of MTX injection and cyclosporine. Pustules disappeared after seven days of drug administration. This case was the first combination therapy with MTX injection and cyclosporine used during the period of 19 May to 26 May 2017 at the Department of Dermatology and Venereology Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital Bandung, resulting in good results. [MKB. 2017;49(3):208–12]Key words: Cyclosporine, generalized pustular psoriasis, methotrexate, methotrexate injection
Efikasi Terapi Kombinasi Salep Kalsipotriol 0,005% dan Klobetasol Propionat 0,05% Dibanding Klobetasol Propionat 0,05% pada Psoriasis Vulgaris Indriyani, Dian; Sutedja, Endang; Suwarsa, Oki
Majalah Kedokteran Bandung Vol 42, No 1
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Psoriasis adalah penyakit eritropapuloskuamosa kronik residif. Obat yang paling sering digunakan untuk terapi penyakit ini adalah kortikosteroid topikal, tetapi penggunaan jangka panjang dapat menyebabkan berbagai efek samping. Kalsipotriol merupakan obat topikal selain kortikosteroid yang memiliki efek samping yang lebih ringan. Tujuan penelitian ini adalah untuk membandingkan skor psoriasis area and severity index (PASI) sebelum dan sesudah pengobatan dengan kombinasi salep kalsipotriol 0,005% dengan salep klobetasol propionat 0,05% dan monoterapi salep klobetasol propionat 0,05% pada penderita psoriasis vulgaris. Skor psoriasis area severity index (PASI), digunakan untuk mengukur berat ringannya psoriasis dan mengevaluasi perbaikan lesi. Desain penelitian adalah uji klinik dengan rancangan acak secara double blind. Uji Mann-Whitney dan Wilcoxon digunakan untuk menganalisis data skor PASI sebelum dan sesudah pengobatan. Penelitian dilakukan di poliklinik I.K. Kulit danKelamin RS. Hasan Sadikin Bandung dari bulan November 2007- Januari 2008 dengan subjek penelitian sebanyak 44 orang yang dibagi dalam dua kelompok. Derajat keparahan penyakit dinilai dengan skor PASI yang dinilai sebelum, satu minggu, dan dua minggu setelah pengobatan. Kesimpulan: Penggunaan terapi kombinasi salep kalsipotriol 0,005% dan klobetasol propionat 0,05% selama dua minggu memiliki efikasi yang lebih baik dibandingkan monoterapi salep klobetasol propionat 0,05%, secara statistik sangat bermakna (p<0,001).Efficacy Combination Therapy of 0.005% Calcipotriol and 0.05% Clobetasol Propionat Compared to 0.05% Clobetasol Propionat Ointment in Psoriasis VulgarisPsoriasis is a chronic relapsing erythropapulosquamous skin disease. Topical corticosteroid is the most common treatment for psoriasis, but it can cause many side effect on longterm used. Calcipotriol ointment is the other topical treatment for psoriasis and has minimal side effect. The study was a double blind randomized clinical trial, compared psoriasis area and severity index score before and after treatment combination therapy of 0.005% calcipotriol ointment 0.05% and clobetasol propionat ointment with 0.05% clobetasol propionat alone. Psoriasis area severity index (PASI) score was used to evaluate severity degree and imprvoment of psoriasis. Mann-Whitney and Wilcoxon test were used for statistical analysis of PASI score before and after treatment. Study was conducted at Dermatology and Venereology outpatient Department of Hasan Sadikin Hospital Bandung period November 2007 to January 2008 with fourty four psoriasis vulgaris patients were enrolled in this study, divided into two groups. The assesment of the severity of skin lesions psoriasis area and severity index was performed at baseline, after one week and two weeks treatment. Conclusion: the use of daily 0.005% calcipotriol ointment combined with 0.05% clobetasol propionate. DOI: http://dx.doi.org/10.15395/mkb.v42n1.4
Imunitas Bawaan pada Kusta: Hubungan Ekspresi Kaspase-1 dengan Interleukin-18 pada Lesi Kulit Pasien Kusta Suwarsa, Oki; Gunawan, Hendra; Achdiat, Pati Aji
Majalah Kedokteran Bandung Vol 48, No 3 (2016)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v48n3.849

Abstract

Respons imun terhadap Mycobacterium leprae (M. leprae) terdiri atas respons imun bawaan dan didapat. Salah satu enzim yang berperan pada imunitas bawaan adalah kaspase-1 dengan fungsi memecah sitokin proinflamasi prointerleukin (IL)-18 menjadi IL-18 aktif. IL-18 bersama IL-12 secara sinergis akan merangsang produksi interferon (IFN-γ) sehingga timbul respons imun seluler yang bersifat protektif. Tujuan penelitian ini adalah menganalisis korelasi positif ekspresi kaspase-1 dengan IL-18 pada lesi kulit pasien kusta. Penelitian dilaksanakan selama bulan Oktober–Desember 2014 di Poliklinik Morbus Hansen Departemen Ilmu Kesehatan Kulit dan Kelamin serta Laboratorium Imunohistokimia Departemen Patologi Anatomi Fakultas Kedokteran Universitas Padjadjaran/Rumah Sakit Dr. Hasan Sadikin Bandung. Penelitian ini merupakan penelitian klinis analitik observasional dengan rancangan potong lintang. Subjek penelitian berjumlah 19 pasien kusta yang didapatkan melalui consecutive sampling. Terhadap subjek penelitian dilakukan anamnesis, pemeriksaan fisis, pemeriksaan bakteriologis, dan biopsi plong. Pada jaringan hasil biopsi lesi kulit dilakukan pemeriksaan imunohistokimia ekspresi kaspase-1 dan IL-18 yang dinilai dengan histoscore, kemudian dilakukan analisis korelasi menggunakan uji Rank-Spearman. Pada hasil penelitian ini didapatkan nilai ekspresi histoscore kaspase-1 pada lesi kulit pasien kusta berturut-turut tipe tuberkuloid (TT)=12,00; borderline tuberculoid (BT)=8,33; mid-borderline (BB)=8,00; borderline lepromatous (BL)=4,00; dan lepromatous (LL)=10,67. Nilai histoscore ekspresi IL-18 pada lesi kulit pasien kusta berturut-turut pada tipe TT=9,33; BT=6,50; BB=4,60; BL=4,00; dan LL=9,33. Berdasarkan hasil analisis statistik uji Rank-Spearman pada derajat kepercayaan 95% didapatkan korelasi positif yang bermakna antara ekspresi histoscore kaspase-1 dan IL-18 (rs=0,618; p=0,005). Simpulan penelitian ini adalah terdapat korelasi positif antara ekspresi kaspase-1 dan IL-18 pada lesi kulit pasien kusta. Semakin tinggi ekspresi kaspase-1 pada lesi kulit pasien kusta maka semakin tinggi ekspresi IL-18. [MKB. 2016;48(3):181–6]Kata kunci: Imunitas bawaan, interleukin-18, kaspase-1, kustaInnate Immunity in Leprosy: Correlation between Caspase-1 and Interleukin-18 Expression in Leprosy Patient’s Skin Lesion Immune response against M. leprae contains innate and adaptive immunity. Caspase-1 is one of the enzymes which have a role in innate immunity to activate pro-IL-18 into its active form. IL-18 and IL-12 sinergically enhance the production of IFN-γ which triggers the protective cellular immunity. The aim of this study was to analyze the positive correlation between the expression of caspase-1 and IL-18 in leprosy patients’ skin lesions. The study was conducted from October tol December 2014 at the Dermatology and Venereology, and Immunohistochemistry Laboratory, Pathology Anatomy Department, Universitas Padjadjaran/Dr. Hasan Sadikin General Hospital Bandung. This was a clinical observational analytical study with cross-sectional design. Subjects were 19 leprosy patients recruited through consecutive sampling. History taking, physical examination, bacteriological examination, and punch biopsy were performed on all subjects. Caspase-1 and IL-18 expressions were examined and measured with histoscore. Correlation analysis was performed using Rank-Spearman test. The histoscore for the expression of caspase-1 in leprosy patients’ skin lesions was as follows: TT type=12.00; BT=8.33; BB=8,00; BL=10.00; and LL=10.67. The histoscore for the IL-18 expression in leprosy patients’ skin lesions were TT type=9.33; BT=4.60; BL=4.00; and LL=9.33. Rank-Spearman analysis with desired confidence level of 95% showed a statistically significant positive correlation between the histoscore of caspase-1 expression and IL-18 expression.(rs=0.618, p=0.005). In conclusion, this study demonstrates a positive corelation between caspase-1 expression and IL-18 expression in leprosy skin lesions. An increase of caspase-1 expression indicates an increase of IL-18 expression. [MKB. 2016;48(3):181–6]Key words: Caspase-1, interleukin-18, innate immunity, leprosy
Methicillin-Resistant Staphylococcus aureus pada Penderita Dermatitis Atopik dan Sensitivitasnya terhadap Mupirosin Dibandingkan dengan Gentamisin Istasaputri M., Keni; Sutedja, Endang; Suwarsa, Oki; Sudigdoadi, Sunarjati
Majalah Kedokteran Bandung Vol 45, No 1 (2013)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) mulai dijumpai pada penderita dermatitis atopik (DA) derajat sedang hingga berat dan bersifat multiresisten terhadap antibiotik topikal. Gentamisin telah digunakan secara luas, sedangkan mupirosin merupakan pilihan utama eradikasi MRSA. Penelitian ini bertujuan untuk mengetahui kolonisasi MRSA pada penderita DA dan sensitivitasnya terhadap mupirosin dibandingkan dengan gentamisin di Poliklinik Departemen Ilmu Kesehatan Kulit dan Kelamin Rumah Sakit Umum Pusat Dr. Hasan Sadikin Bandung. Survei potong lintang analitik dilakukan selama 19 September–31 Oktober 2011 terhadap 22 penderita DA derajat sedang hingga berat sesuai dengan Scoring Atopic Dermatitis objektif dan 22 individu sehat. Seluruh subjek dilakukan pemeriksaan apusan kulit untuk deteksi MRSA dan uji sensitivitas terhadap mupirosin dan gentamisin. Pada kedua kelompok masing-masing didapatkan 10 laki-laki dan 12 perempuan. Pada kelompok penderita DA didapatkan DA derajat sedang (10/22) dan berat (12/22), dengan lesi akut (14/22) dan kronik (8/22). Pertumbuhan S. aureus didapatkan pada 17/22 penderita DA dengan sensitivitas terhadap mupirosin 14/17 dan gentamisin 11/17 penderita. Pada individu sehat tidak didapatkan pertumbuhan koloni S. aureus. Galur MRSA ditemukan pada 3/17 penderita DA dan dianggap berbeda secara bermakna bila dibandingkan dengan individu sehat (p<0,01), galur ini bersifat sensitif terhadap mupirosin pada 2/3 penderita dan seluruhnya resisten terhadap gentamisin. Simpulan, kolonisasi MRSA perlu dicurigai pada penderita DA derajat sedang hingga berat, sehingga perlu pengobatan tambahan antibiotik topikal mupirosin. [MKB. 2013;45(1):36–44]Kata kunci: Dermatitis atopik, gentamisin, MRSA, mupirosinMethicillin-Resistant Staphylococcus aureus in Atopic Dermatitis Patients and Its Sensitivity to Mupirocin Compared to GentamycinMethicillin-resistant Staphylococcus aureus (MRSA) is found in moderate to severe atopic dermatitis (AD) and is multiresistant against topical antibiotic. Gentamycin is widely used while mupirocin is the first line therapy to eliminate MRSA. This research is intended to observe the colonization of MSRA case in AD patients and its sensitivity to mupirocin compared to gentamycin in Dermato-venereology Clinic Dr. Hasan Sadikin General Hospital Bandung. An analytical cross sectional survey was intended from 19th September–31st October 2011 in 22 moderate to severe AD patients based on objective Scoring Atopic Dermatitis and 22 healthy persons. All subjects went through skin-scraping, MSRA was detected and sensitivity test was done for mupirocin and gentamycin. The subjects consisted of 10 boys and 12 girls in both group. Based on severity, 10/22 of AD subjects were in moderate AD and 12/22 were severe AD, 14/22 of AD subjects presented with acute lesions and 8/22 subjects had chronic lesions. In the group of AD patients, 17/22 growth of S. aureus were found with sensitivity against mupirocin 14/17 and gentamycin 11/17. In healthy individual group, no  S. aureus growth was found. Methicillin-resistant Staphylococcus aureus strain in AD patients was observed on 3/17 patients, and this was declared significantly different compared to healthy individuals (p <0.01). The isolates in 2/3 patients were sensitive to mupirocin and all patients were resistant to gentamycin. In conclusion, the patients of moderate to severe AD are prone to MRSA colonization and therefore requires mupirocin topical antibiotic treatment.  [MKB. 2013;45(1):35–43]Key words: Atopic dermatitis, gentamycin, MRSA, mupirocin DOI: http://dx.doi.org/10.15395/mkb.v45n1.203
Co-Authors - Sudigdoadi, - Achadiyani Achmad Yudha Pranata, Achmad Yudha Achmad, Fariz Andrew Citrawan Dany Hilmanto Dedi Rachmadi Sjambas Devi Nursjamsi, Nadilla Carissa Dian Indriyani Dida Akhmad Gurnida Dida Akhmad Gurnida Dimas Erlangga Effendi, Rendy Ariezal Endang Sutedja Endang Sutedja Endang Sutedja Endang Sutedja Endang Sutedja Erda Avriyanti Erfina Rohana Sormin Eva Sri Rahayu Farid Husin Fatima Aulia Khairani Fenny Dwiyatnaningrum Fenny Dwiyatnaningrum, Fenny Feriza, Vina Firman Fuad Wirakusumah Gaga Irawan Nugraha Hadi Susiarno Hadi Susiarno Hartati Purbo Dharmadji Hartati Purbo Dharmadji Hartati Purbo Dharmadji, Hartati Purbo Hartati Purbo Dharmaji Hartati Purbo Dharmaji, Hartati Purbo Hendra Gunawan Insi Farisa Desy Arya Intan Karlina Irna Sufiawati Jayawarsa, A.A. Ketut Karinna Dwi Purnama Keni Istasaputri M. Lasiyati Yuswo Yani Maryam Nissa Hazari Maulinda, Shinta Metha Solihati Rayuna Miranti Pangastuti Muhammad Akbar Wicaksana Nanan Nur'aeny Nizami Hamada Nur Atik Nur'aeny, Nanan Nurwinda Saputri Pangastuti, Miranti Pati Aji Achdiat Ponpon Idjradinata Pratiwi, Auria Ekanti Purnama, Karinna Dwi Puteri Nabilah Maharani Raden Mohamad Rendy Ariezal Effendi Raden Tina Dewi Judistiani Rasmia Rowawi Reiva Farah Dwiyana Reti Hindritiani Ronny Lesmana Ruchiatan, Kartika Saputri, Nurwinda Siti Nurjanah Sjambas, Dedi Rachmadi Sri Mulyati Sudigdo Adi Sudigdo Adi Sunarjati Sudigdoadi Sunaryati S. Adi Susantina Prodjosoewojo Susiarno, Hadi Syawalika Ulya Isneny Tenny Putri Tiara Rica Dayani Uni Gamayani Vita Murniati Tarawan Vita Murniati Tarawan Vita Murniati Tarawan Wicaksana, Muhammad Akbar Yogi Faldian Yuli Kurniawati Yuni Susanti Pratiwi