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All Journal International Journal of Educational Narratives Journal of Computer Science Advancements Journal of Moeslim Research Technik Journal of Selvicoltura Asean Research Psychologie, Orientation et Conseil Journal of Tecnologia Quantica Techno Agriculturae Studium of Research Research of Scientia Naturalis Journal of Humanities Research Sustainability
Zain Nizam
Universiti Malaysia Sarawak
Aerospace Engineering Agriculture, Biological Sciences & Forestry Humanities Automotive Engineering Chemistry Computer Science & IT Control & Systems Engineering Education Electrical & Electronics Engineering Engineering Physics Social Sciences Other

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CRISPR-CAS9 MEDIATED GENETIC ENHANCEMENT OF DROUGHT TOLERANCE IN MAIZE: A MOLECULAR AND FIELD VALIDATION STUDY Muhammad Hazmi; Zain Nizam; Nina Anis
Research of Scientia Naturalis Vol. 2 No. 3 (2025)
Publisher : Yayasan Adra Karima Hubbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70177/scientia.v2i3.2382

Abstract

Drought is a primary constraint on maize (Zea mays L.) productivity worldwide, threatening global food security. The development of climate-resilient crops is therefore a critical priority. This study aimed to enhance drought tolerance in maize by utilizing the CRISPR-Cas9 system to edit a key negative regulator gene involved in the drought stress response pathway. The research employed an Agrobacterium-mediated transformation method to introduce the CRISPR-Cas9 construct into maize embryos. Putative edited plants were rigorously screened using molecular techniques, including PCR and Sanger sequencing, to confirm successful gene modification. Validated T1 generation lines were then subjected to controlled drought stress conditions in greenhouse trials and subsequently evaluated in multi-location field trials. The edited maize lines exhibited significantly improved physiological and agronomic performance under water-deficit conditions, including enhanced photosynthetic efficiency, reduced leaf water loss, and a 15-20% increase in grain yield compared to non-edited wild-type controls. This study demonstrates the efficacy of CRISPR-Cas9 for developing drought-tolerant maize, offering a precise and rapid strategy for crop improvement to mitigate the impacts of climate change.
"Babad Tanah Jawi" as an Ecological Archive: A Philological Study of Ancient Javanese Manuscripts for Records of Climate and Calamity Wijaya Wijaya; Zain Nizam; Aiman Fariq
Journal of Humanities Research Sustainability Vol. 2 No. 5 (2025)
Publisher : Yayasan Adra Karima Hubbi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70177/jhrs.v2i5.2780

Abstract

Background. Environmental history in the Indonesian archipelago has often relied on geological data, colonial records, and contemporary environmental reports, leaving indigenous textual traditions underexamined as potential sources of ecological knowledge. Babad Tanah Jawi, a key corpus of ancient Javanese historiographical manuscripts, contains rich descriptions of natural events, celestial signs, and social responses to environmental disruptions, yet its value as an ecological archive has not been systematically explored. Purpose. This study aims to investigate how these manuscripts document patterns of climate variability, environmental calamities, and ecological transformations, and to assess their relevance for reconstructing long-term environmental change in Java. Method. A philological research design was employed to analyze selected manuscript variants through textual criticism, intertextual comparison, and thematic ecological coding. The analysis identified recurrent references to volcanic eruptions, prolonged droughts, exceptional rainfall, crop failures, and shifts in river courses, which align with known geological and climatological data. Results. The findings also highlight indigenous interpretive frameworks that connect environmental disturbances with moral, cosmic, and political meanings, revealing the epistemological foundations through which premodern Javanese societies understood ecological instability. Conclusion. The study concludes that Babad Tanah Jawi offers valuable supplementary evidence for reconstructing historical climate patterns while also enriching contemporary environmental humanities through its integration of cosmology, ecology, and social memory. These insights underscore the importance of indigenous manuscripts as cultural-ecological repositories that can broaden interdisciplinary approaches to climate history, disaster studies, and resilience research.  
Co-Authors Achmad Ridwan Aiman Fariq Bambang Winardi Busnawir Busnawir Chevy Herli Sumerli Daniyar Satybaldy Enda Wista Sinuraya Fatima Malik Haziq Idris Hazmi, Muhammad Liz Yanti Andriyani Muhammad Arif Abdul Hakim Nina Anis Nong Chai Nurul Huda Rashid Rahman Rina Farah Sara Hussain Siska Putri Belangi Siti Aisyah Sun Wei Syaifullah Rahim Wijaya Wijaya