Garuda - Garba Rujukan Digital

p-Index From 2021 - 2026

2.326

P-Index

This Author published in this journals

All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences The Journal of Experimental Life Sciences (JELS) Biotropika Biology, Medicine, & Natural Product Chemistry Molecular and Cellular Biomedical Sciences (MCBS) Makara Journal of Science Jurnal Kefarmasian Indonesia Annales Bogorienses

Rifa'i, Muhaimin

Unknown Affiliation

Author-ID : 1097221

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Dentistry Environmental Science Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

Published : 37 Documents Claim Missing Document

Claim Missing Document

Articles

1 2 3 4

EMSA Eritin Blocks Nuclear Factor-Kappa B in Diabetes Mellitus Mice Model Sihombing, Maic Audo Lin; Sumitro, Sutiman B.; Rifa'i, Muhaimin
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Graduate School, Universitas Brawijaya

Diabetes mellitus is one of the common metabolic disorders with increasing prevalence during recent years with hyperglycemia as its characteristic. DM has been shown to be a state of free radicals over production resulted from hyperglycemia that can activate cellular signaling pathways transcription of factor NF-κB which stimulates the production of several inflammatory mediators andl lead to chronic inflammation. Chronic inflammation is implicated in β-cell damage and function and promotes apoptosis. EMSA Eritin is a polyherbal consisting of soy bean extracts, coconut water extract and red rice extract that assumed to be antidiabetic and anti-inflammatory. This study will assess the effectiveness of EMSA Eritin against inflammation in diabetes mellitus by measuring levels of NF-κB produced by immunocompetent cells in DM mice model. Streptozotocin 100 mg.kg-1 BW is used to induce diabetes mellitus in mice. Oral administration of EMSA Eritin was given for 14 days with dose of 0.3125 mg.g-1 BW, 3.125 mg.g-1 BW and 31.25 mg.g-1 BW. Data were analyzed using One-way ANOVA (p<0.05) and Duncan test using SPSS 16.0 for Windows. The results showed that EMSA Eritin can be used as an alternative therapy for the treatment of DM. The level of NF-κB in diabetic mice significantly decreased when the mice received EMSA Eritin. Keywords: Diabetes Mellitus, EMSA Eritin, NF-κB, ROS

Anti-hyperglycemic and Immunomodulatory Activity of a Polyherbal Composed of Sesbania grandiflora, Salacca zalacca and Acalypha indica Zamroni, Ahmad; Zubaidah, Elok; Rifa'i, Muhaimin; Widjanarko, Simon Bambang
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2018.008.03.09

Diabetes has become a serious global public health problem due to its high prevalence and mortality. Unfortunately, current anti-diabetic drugs are having some limitations and adverse effects. Therefore, searching for a new anti-diabetic agent is an urgent challenge. In this research, we examined the effectiveness of a traditional anti-diabetic polyherbal composed of Sesbania grandiflora seeds, Salacca zalacca leaves and Acalypha indica roots (2:1:1). The study was aimed to explore the anti-hyperglycemic effect of the polyherbal in STZ-induced diabetic mice and to investigate the immunomodulatory activity involved in the process of controlling hyperglycemia. Our results showed that the polyherbal water extract (150 mg.kgBW-1) could suppress blood glucose elevation and preserve pancreatic islet of diabetic mice. Moreover, the polyherbal treatment could normalize the relative amount of activated CD4+CD62L- and CD8+CD62L- T cells. The polyherbal extract also stimulated the production of CD4+CD25+FoxP3+ regulatory T cells (Tregs) which is known to play an important role in diabetes control. In addition, polyherbal treatment also increased the relative amount of anti-inflammatory cytokines IL-10 and TGF-β. These results revealed that the polyherbal extract has an anti-hyperglycemic and immunomodulatory activity that may provide beneficial function in diabetes healing. Keywords: Antidiabetes, Immunomodulator, Polyherbal, Regulatory T cell.

Combination Moringa oleifera Extract and Ifalmin as Potential Formulation of Preventing Inflammation in Diabetic Mice Model Nilamsari, Ruri Vivian; Adharini, Wahyu Isnia; Lestari, Noviana Dwi; Tsuboi, Hideo; Rifa'i, Muhaimin
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2019.010.01.07

In Indonesia, the prevalence of diabetes mellitus hits 6.2%, making Indonesia one of the top ten diabetes mellitus countries. Efforts to prevent and treat people with diabetes in Indonesia are required to minimize that as well. One is through treatment with local herbal products such as Moringa oleifera (MO) and Toman fish extract (Channa micropeltes), called Ifalmin. The aim of this research is to investigate the potential role of a combination of Extract Moringa oleifera and Ifalmin to reduce inflammation in diabetes conditions. Diabetic mice were done by Streptozotocin (STZ) induction with a single-dose 145 mg.kg-1.Then, diabetic mice were given an oral treatment of combination MO extract and Ifalmin for 14 days. In this experiment combinations of MO extract and Ifalmin are divide into 3 dose, There are: dose 1 (800 mg.kg-1 : 800 mg.kg-1), dose 2 (650 mg.kg-1 : 650 mg.kg-1), and dose 3 (800 mg.kg-1 : 650 mg.kg-1). Immune cells originate from the spleen are stained by immunofluorescence antibodies and analyzed by flow cytometry with BD Cellquest ProTM software. The results showed an increase of expression pro-inflammatory cytokines IL-1β and IL-6 in diabetic mice compared to normal control. Only dose 1 and dose 2 has shown the capability to reduce the expression of IL-1β in diabetic mice. But, the combination of MO and Ifalmin has an antagonist effect on the expression of IL-6. The inhibitory mechanism can be assumed by the action of antioxidant compounds (Flavonoids and Alkaloids) in MO and Albumin compound in Ifalmin. Those combination act as exogenous antioxidant that help endogenous inside the body. A combination of MO extract and Ifalmin with a certain dosage was able to decrease proinflammatory cytokines IL-1β on the cells involved in innate immunity.

The Role of Moringa oleifera- Ifalmin® Formulation in Regulation of B220+IgM+ and B220+IgG+ in Diabetic Mice Adharini, Wahyu Isnia; Nilamsari, Ruri Vivian; Lestari, Noviana Dwi; Widodo, Nashi; Rifa'i, Muhaimin
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Graduate School, Universitas Brawijaya

It has been known that the immunoglobulin levels were altered in diabetes mellitus (DM) conditions. This study aimed to evaluate the levels of immunoglobulins in DM mice after the administration of Moringa oleifera-Ifalmin® formulation (MI). Streptozotocin, at a dose of 145 mg.kg-1, was injected intraperitoneally to experimental mice to obtain diabetic mice. The groups were divided into normal mice, diabetic mice without treatment, diabetic mice with metformin treatment (307.5 mg.kg-1 BW), and diabetic mice with MI treatment at dose 1 (M:I= 800 mg.kg-1 BW: 800 mg.kg-1 BW), dose 2 (M:I= 615 mg.kg-1 BW: 615 mg.kg-1 BW), and dose 3 (M:I= 800 mg.kg-1 BW: 615 mg.kg-1 BW). Mice were orally treated by MI for 14 days. Subsequently, the levels of immunoglobulin IgM and IgG were evaluated using flow cytometry analysis. IgM and IgG levels were significantly lower in the DM group than the normal group. These results indicated that DM altered immunoglobulin levels. MI treatment for 14 days significantly increased the number of IgM and IgG at the level equivalent to the normal group and significantly different as compared to the DM group. Based on the results, MI can be used as an immunomodulatory agent in humoral immunity through the precise regulation of IgM and IgG.

The Effectiveness of Combination of Momordica charantia Extract and Averrhoa bilimbi on Nf-Kb Activation in Mice (Mus musculus) Balb/C Hyperglycemia Models Sampurna, Intan Sartika Risky; Rifa'i, Muhaimin; Rahayu, Sri
The Journal of Experimental Life Science Vol. 10 No. 1 (2020)
Publisher : Graduate School, Universitas Brawijaya

Hyperglycemia is a medical condition when the blood glucose levels increase exceeds the reasonable limit. Hyperglycemia is a typical sign of diabetes mellitus (DM). Indonesia is the sixth-ranked country in the world after China, India, United States, Brazil, and Mexico. Treatment with herbs is currently being developed. Pare (Momordica charantia) and Belimbing wuluh (Averrhoa bilimbi) are plants that found around us. Some studies state that each of these plants can be anti-diabetic. The hyperglycemia can cause an immune system disorder characterized by pancreatic β cell death involving IL-1β, kappa B (NF)-κB nuclear factor, and Fas. The ability of NF-kB activation will affect the number of cytokines expressed by T cells, namely TNF-α, and IFN-γ. The purpose of this study is to determine the effect of NF-kB activation on blood glucose levels in hyperglycemia mice. The results showed that the positive control treatment showed an increase in the number of NF-kB activations in CD4 and CD8 cells. EPBW (combination of Averrhoa bilimbi extract and Momordica charantia) administration results at a dose of 10 mg.kg-1 BW showed a reduction in the amount of activated NF-kB as a substitute for the reduction. In addition, that dose can reduce blood sugar levels in mice hyperglycemia model.

Effects of Bitter Melon (Momordica charantia L.) and Starfruit (Averrhoa bilimbi L.) on Proinflammatory Cytokines Produced by Hyperglycemic Mice Model Wardani, Bella Pradina Novinda; Rahayu, Sri; Rifa'i, Muhaimin
The Journal of Experimental Life Science Vol. 10 No. 2 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.02.03

Hyperglycemia is a condition of excessive blood glucose in blood plasma caused by damage to the pancreatic beta-cell structure that causes impaired insulin secretion. This study aimed to investigate the combination of Bitter Melon and Star Fruit ethanol extract (SBME) on pro-inflammatory cytokines production in hyperglycemic albino mice models. This study was treated in 25 female albino mice weighing 25-30 and 9 weeks old. STZ was given in a dose of 145 mg.kg-1 BW intraperitoneally. Hyperglycemic mice were given orally with SBME ethanol extract at doses 10, 40, and 160 mg.kg-1 BW for two weeks. After treatment, pro-inflammatory molecules were analyzed by flow cytometry from the splenic cell. This study showed that SBME treatment can reduce the production of pro-inflammatory cytokines especially IL-1b, but increases IL-6 in hyperglycemic mice. After treatment with SBME at a dose of 40, the IL-1b pro-inflammation molecule decreased significantly (p>0,05). It reached a normal physiological level, but the dose has not been able to reduce the IL-6 pro-inflammation molecule significantly. The conclusion of this study was Bitter Melon and Star Fruit ethanol extract (SBME) with a treatment of 40 mg.kg-1 BW can suppress IL-1b pro-inflammatory cytokines in hyperglycemic mice models, but has not been able to reduce the expression of IL-6 pro-inflammatory cytokines. Suggesting this medicinal herb might be a useful strategy for future therapeutic interventions in degenerative diseases or diseases involving cell activation, but a study of doses is needed.Keywords: Averrhoa bilimbi L., Hyperglycemia, Inflammation, Momordica charantia L.

The Study of Combination Ethanol Extract of Averrhoa bilimbi L. and Momordica charantia L. on CD4+CD25+TGF-β+ Spleenocytes of Hyperglycemia Mice Amrulloh, Harits; Novinda, Bella; Sartika, Intan; Widyarti, Sri; Rifa'i, Muhaimin
The Journal of Experimental Life Science Vol. 10 No. 3 (2020)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2020.010.03.05

Diabetes mellitus is one of the four priority non-infectious diseases in the world. Plant-based medicine is an alternative treatment with few side effects. Star fruit (Averrhoa bilimbi L.) and bitter melon (Momordica charantia L.) are plants that have anti-hyperglycemic activity. Hyperglycemia produces Reactive Oxygen Species (ROS) that make the β-cells of the pancreas necrosis that decreasing insulin synthesis. The anti-inflammatory activity appears based on the relative levels of CD4+ and CD25+, which are TGF-β-producing regulatory T cells where TGF-β is a mediator that acts as an immunosuppressant. TGF-β would induce CD4 + T lymphocytes into T reg. The purpose of this study is to determine the profile of TGF-β on CD4+ and CD25+ spleenocytes on hyperglycemia mice after ABMC (Averrhoa bilimbi Momordica charantia mix) treatment. Mice were divided into 5 groups, non-diabetic (N), hyperglycemia (H), hyperglycemia with extract doses of 10 mg.kg-1 BW (P1), 40 mg.kg-1 BW (P2), and 160 mg.kg-1 BW (P3). Diabetic mice were obtained after a single injection dose of 145 mg.kg-1 BWÂ streptozotocin (STZ). The result showed that ABMC can reduce blood sugar levels faster and able reduce the number of CD4+TGF-β+ cells in hyperglycemia mice. Keywords: Averrhoa bilimbi L., CD4+TGF-β+, CD25+TGF-β+, hyperglycemia, Momordica charantia L.

Co-Authors Ade MC Rohie, Ade MC Adharini, Wahyu Isnia Aisyah Zahroh Aden Al Faizah, Belinda Nabiila Al-Faizah, Belinda Nabiila Amrulloh, Harits Andi Rizki A, Pradana Andi Rizki Adi Pradana, Andi Rizki Adi Ardiansyah, Esha Aris Soewondo Askandar Tjokroprawiro Atho'illah, Mochammad Fitri Atho’illah, Mochammad Fitri Dia Faroka Dinia Rizqi Dwijayanti Djanggan Sargowo Djati, Mohammad Sasmito Elok Zubaidah Erin Kurnianingtyas Estri Laras Arumingtyas Fadlilah, Dawama Nur Farida Rachmawati, Farida Fatinah, Arik Arubil Indriya Rachmawati Izati, Rahmi Izzah, Fathiyah Nurul Kavitarna, Septhyanti Aprilia Khodijah, Riska Amalia Khoirin Maghfiroh Lestari, Noviana Dwi Maic Audo Lin Sihombing Moch Sasmito Djati Mutya Farsely Nashi Widodo Nilamsari, Ruri Vivian Novinda, Bella Sa'adah, Nur Alfi Maghfirotus Sakti, Muhammad Wisam Wira Sakti, Sefihara Paramitha Sampurna, Intan Sartika Risky Sari, Fikriya Novita Sartika, Intan Shinta Oktya Wardhani, Shinta Oktya Shofiyah, Aya SIMON BAMBANG WIDJANARKO Siti Nur Arifah, Siti Nur SRI RAHAYU Sri Widyarti Susanti, Winda Karina Sutiman B. Sumitro Totowiputro, Dicky Kurniawan Tsuboi, Hideo Wahyuningsih, Nadia Wardani, Bella Pradina Novinda Widekdo, Dwi purno Wisnu Barlianto YOGA DWI JATMIKO Yuyun Ika Christina Zamroni, Ahmad

Title

Found 37 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 37 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 37 Documents
Search