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Juniarti .
Magister Sains Biomedis, Sekolah Pascasarjana, Universitas YARSI, Jakarta
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Journal : Makara Journal of Science

 1 
Effects of Methanolic Jatropha multifida L. Extract in Wound Healing Assessed by the Total Number of PMN Leukocytes and Fibroblasts Juniarti, Juniarti; Aryenti, Aryenti; Yuhernita, Yuhernita; Poerwaningsih, Ernie Ernie
Makara Journal of Science Vol. 16, No. 3
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The aim of this study was to evaluate the effects of methanol extract of Jatropha multifida leaves on the wound healing process and to investigate the wound healing activity based on reduced numbers of PMN (polymorpho nuclear) leukocytes and increased numbers of fibroblasts. Methanol extract of dried leaves of J. multifida was used in the wound healing activity studies. The study subjects were 36 white male Sprague Dawlay rats aged 2 months with 150-200 gram body weight. The subjects were divided into 4 groups and experimentally injured: Group I (negative control) underwent injury without subsequent treatment; group II (positive control) received topical treatment with Bethasone-N after injury; group III (solvent control) was treated with 70% methanol; group IV (treatment group) was treated with 10 mg methanol extract of J. multifida Each group consisted of 3 rats, which were decapitated on days 3, 6, and 13 after the start of treatment. Histological preparation was stained with hematoxyline-eosin (HE) and was continuously examined by counting the numbers of PMN leukocytes and fibroblasts as indicators of wound healing on days 3, 6, and 13 of treatment. The study showed lower numbers of PMN leukocytes in subjects treated with the extract of J. multifida as compared to the other groups. The numbers of fibroblasts were significantly higher on days 6 and 13 of treatment. The treatment of injuries with methanol extract of leaves from J. multifida provided better results compared to the other groups in our study.
KANDUNGAN SENYAWA KIMIA, UJI TOKSISITAS (Brine Shrimp Lethality Test) DAN ANTIOKSIDAN (1,1-diphenyl-2-pikrilhydrazyl) DARI EKSTRAK DAUN SAGA (Abrus precatorius L.) Juniarti, Juniarti; Osmeli, Delvi; Yuhernita, Yuhernita
Makara Journal of Science Vol. 13, No. 1
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Chemical Compound Gynecology, ToxinTest (Brine Shrimp Lethality Test) and Antioxidants (1,1-Diphenyl-2- Pikrilhydrazyl) from Saga Leaf Extract (Abrus Precatorius L.). Cytotoxicity effects and antioxidant activities of saga (Abrus precatorius L.) extract were studied by using Brine Shrimp Lethality Test and 1,1-diphenyl-2-pikrilhydrazyl assays. Saga was extracted with different solvents i.e n-hexane, ethyl acetate and methanol. Result showed that the methanolic extract of saga had been potential to be cytotoxic againts Brine Shrimp with LC50 value of 606.736 ppm. However, all the extract of saga did not have antioxidant activities.
ANALISIS SENYAWA METABOLIT SEKUNDER DARI EKSTRAK METANOL DAUN SURIAN YANG BERPOTENSI SEBAGAI ANTIOKSIDAN Yuhernita, Yuhernita; Juniarti, Juniarti
Makara Journal of Science Vol. 15, No. 1
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Secondary Metabolites Analysis of Methanol Extract of Surian (Toona sureni (Bl.) Merr) Leaf as Antioxidant Potential. The study of performed secondary metabolites from the methanol extract of Surian (Toona sureni (Bl.) Merr) leaves have been done by thin-layer chromatography (TLC) method. The result showed that methanol extract of Surian leaves consist of alkaloid, flavonoid, polyphenol and terpenoid. All of them positively have the ability to scavenge 2,2- diphenyl-1-picrylhydrazyl (DPPH). It has IC50 (4.80) are smaller than the ascorbat acid standard (IC50 = 9.23).
DESTILASI MINYAK ATSIRI DAUN SURIAN SEBAGAI KRIM PENCEGAH GIGITAN NYAMUK Aedes aegypty L. Juniarti, Juniarti; Yuhernita, Yuhernita; Endrini, Susi
Makara Journal of Science Vol. 15, No. 1
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Distillation of Essential Oil from Surian Leaf (Toona sureni (Bl.) Merr.) as Repellent Cream for Protection Aedes aegypty L. Bites. Isolation of volatile oil of leaf surian (Toona sureni (Bl.) Merr.) have been done by distillation aqueous vapour method. Distillation result had the chocolate colored and stinging aroma, and rendement was 0.23%. GC-MS analysis shown of some compound of terpenoid (naftalen derivated) which correspondence to mass spectrum. One of them is copaene (C15H24), m/z = 204. Cream based of Rajin et al. formula giving stable cream, white coloured and neutral pH. Volatile oil of surian leaf in cream based cause color and aroma cream were changed. Cream stability also was annoyed at oil of atsiri 10%.
Co-Authors Abdussalam, Moch Agustin, Rika Alivia Ahmad Aulia Jusuf Ainur Rahmah, Nunung Aryenti . Aryenti Aryenti Aryenti, Aryenti Delvi Osmeli Dinda, Salsabila Chyllia Erni Hernawati Purwaningsih, Erni Hernawati Ferlianti, Rika Hans -Joachim Freisleben Idris, Muthoharoh Indah Permata Yuda Indah Permata Yuda Indra Kusuma Indra Kusuma Indranita, Lusy Janah, Lulu Ah Lilian Batubara Moch Abdussalam Moch Abdussalam Mohamad Sadikin Nazwirman Nazwirman Nazwirman Nazwirman Nazwirman Nazwirman Nazwirman Nazwirman Nunung Ainur Rahmah Osmeli, Delvi Patrisha, Milaviza Permata Yuda, Indah Poerwaningsih, Ernie Ernie Restu Syamsul Hadi Susi Endrini Susmiarsih, Tripanjiasih Taufik Nasrullah, Taufik Yuda, Indah Permata Yuhemita . Yuhernita . Yuhernita Yuhernita Yuhernita, Yuhernita Yurika Sandra Zainal Zawir Simon