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All Journal Jurnal Pendidikan Matematika dan IPA Biologi Edukasi : Jurnal Ilmiah Pendidikan Biologi INDRA: Jurnal Pengabdian kepada Masyarakat Biofaal Journal Prosiding Konferensi Nasional PKM-CSR ACROPORA: Jurnal Ilmu Kelautan dan Perikanan Papua TROPICAL GENETICS Rumphius Pattimura Biological Journal Tropical Genetics Kalwedo Sains (KASA)
Moniharapon, Mechiavel
Department Of Biology, Faculty Of Mathematics And Natural Science, Universitas Pattimura
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences

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Journal : Biofaal Journal

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POTENSI KULIT BATANG Cinnamomum burmannii Bl. DALAM MENCEGAH INFERTILITAS; KAJIAN TERHADAP BERAT TESTIS DAN JUMLAH SPERMATOZOA MENCIT (Mus musculus) MODEL DIABETES MELLITUS TIPE-1 Moniharapon, Mechiavel; Ukratalo, Abdul M; Pattimura, Nadira; Samson, Efraim; Pangemanan, Victory O
Biofaal Journal Vol 4 No 2 (2023): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v4i2pp108-117

Abstract

In individuals with diabetes mellitus, the increase in Reactive Oxygen Species (ROS) can damage the mitochondrial membrane, leading to the loss of potential mitochondrial membrane function in inducing apoptosis of sperm cells. Cinnamomum burmannii is a type of spice that contains antioxidants, thus capable of neutralizing free radicals and reducing oxidative stress caused by diabetes mellitus. Flavonoids also have an effect on increasing spermatogenesis. The aim of this research is to examine the role of methanol extract from Cinnamomum burmannii bark in increasing testis weight and spermatozoa count in type-1 diabetes mellitus mice (Mus musculus). The study employs a Completely Randomized Design (CRD) with five treatments and three replications. Twenty mice are divided into groups: K- (control), K+ mice injected with STZ without medication), P1 (dose of 200 mg/kg BW), P2 (dose of 400 mg/kg BW), and P3 (dose of 800 mg/kg BW). Before administering the Cinnamomum burmannii bark extract, blood sugar levels are measured in the K+, P1, P2, and P3 groups. If blood sugar levels have increased (>128 mg/dL), the P1, P2, and P3 groups are given the Cinnamomum burmannii bark extract according to the predetermined dosage. The treatment is carried out for 14 days. On the last day, surgery is performed to extract the testes and observe the spermatozoa count. The observation data on testis weight and spermatozoa count will be analyzed using ANOVA with SPSS 24.0. The results indicate that the administration of methanol extract from Cinnamomum burmannii bark significantly affects the increase in testis weight and spermatozoa count in type-1 diabetes mellitus mice. The increase in testis weight and spermatozoa count is proportional to the dosage given
Efficacy Test of Ethanol Extract From Matoa (Pometia pinnata) Stem Bark on Aedes aegypti Larval Mortality As An Effort to Control Dengue Fever Moniharapon, Debby Dijola; Moniharapon, Mechiavel
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp1-9

Abstract

Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by the dengue virus and transmitted primarily through the bite of the Aedes aegypti mosquito, the disease's primary vector. Controlling the population of this mosquito species is considered a strategic measure in preventing the spread of DHF. One potential biological control agent is the matoa plant (Pometia pinnata), a member of the Sapindaceae family widely distributed in tropical regions. This plant contains secondary metabolites such as flavonoids, tannins, and saponins, which exhibit natural insecticidal activity. This study aims to evaluate the efficacy of ethanol extract derived from the bark of P. pinnata against A. aegypti larvae. The extraction process was conducted using ethanol as a solvent, and the resulting extract was tested against mosquito larvae at concentrations of 25 ppm, 50 ppm, 70 ppm, and 90 ppm. Larval mortality data were analysed using Analysis of Variance (ANOVA) via SPSS software version 24.00. The results demonstrated that the ethanol extract of P. pinnata bark had a significant dose-dependent effect on larval mortality. These findings suggest the potential of the extract as a natural active ingredient in the formulation of plant-based larvicides, contributing to environmentally friendly and sustainable dengue vector control strategies.
Expresion of Tumor Necrosis Factor Alfa (TNF-α) in The Pancreas of Diabetic Mice (Mus musculus) Following Adminstration of Catharantus roseus Extract Kaliky, Nunun Ainun Putri Sari Banun; Moniharapon, Mechiavel; Eddy, La
Biofaal Journal Vol 6 No 1 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i1pp10-19

Abstract

Catharanthus roseus is a plant that contains compounds such as alkaloids, flavonoids, saponins, and carotenoids, which act as antioxidants. This study aimed to investigate the effect of Catharanthus roseus extract on the reduction of TNF-α expression in the pancreas of diabetic mice (Mus musculus). In this study, mice with an average body weight of 30 g were induced with streptozotocin (0.1 ml/BB) to induce diabetes mellitus. Subsequently, the diabetic mice were administered Catharanthus roseus extract, using methanol and petroleum benzene solvents at a concentration of 5%, with a dose volume of 1.5 ml/BB, for 14 days. On the final day, the mice were sacrificed, and the pancreas organs were collected, followed by histological preparation using immunohistochemistry methods. The results indicated that administration of Catharanthus roseus extract with methanol and petroleum benzene solvents reduced TNF-α expression. The Catharanthus roseus extract with methanol solvent was more effective in lowering TNF-α expression.
JUMLAH SEL LEYDIG DAN SEL SERTOLI TIKUS GALUR SPARAGUE-DAWLEY TERPAPAR SOPI PASCA DITERAPI EKSTRAK ETANOL SIRIH CINA (Peperomia pellucida L.) Unitly, Adrien Jems Akiles; Killay, Amos; Moniharapon, Mechiavel; Eddy, La; Silahooy, Veince B; Huwae, Laury Marcia Ch; Moniharapon, Debby Dijola; Lakesubun, Bella Frida
Biofaal Journal Vol 5 No 1 (2024): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v5i1pp010-018

Abstract

Tujuan penelitian ini untuk mengetahui jumlah sel Leydig dan sel Sertoli tikus terpapar sopi pasca diterapi ekstrak etanol sirih cina (Peperomia pellucida. L). Rancangan acak lengkap (RAL) digunakan dalam penelitian ini, dengan membagi 15 ekor tikus ke dalam 5 kelompok perlakuan yang diulang sebanyak 3 kali, yaitu kelompok 5.4 adalah 3 ekor tikus yang diberi sopi 5.4 ml/ekor/hari selama 14 hari (kontrol negatif), kelompok Vit. C 6.3 adalah 3 ekor tikus yang diberi sopi 5.4 ml/ekor/hari kemudian diberi Vitamin C 6.3 mg/ekor/hari selama 14 hari (kontrol positif), kelompok 0.71, 1.43 dan 2.86 adalah 3 ekor tikus yang diberi sopi 5.4ml/ekor/hari selama 14 hari kemudian kemudian masing-masing kelompok diberi ekstrak etanol sirih cina 0.71g/ekor/hari selama 14 hari, 1.43g/ekor/hari selama 14 hari dan 2.86g/ekor/hari selama 14 hari. Penelitian ini dilakukan di Laboratorium Zoologi dan Mikroteknik Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Pattimura Ambon mencakup persiapan hewan model, pemberian minuman sopi, ekstraksi sirih cina, pembuatan preparat histologis dan pengamatan. Hasil yang diperoleh dianalisis dengan Analysis of Variance (ANOVA) dan dilanjutkan dengan uji duncan pada taraf nyata a = 0.05 menggunakan perangkat lunak SAS dan dilanjutkan dengan uji beda nyata terkecil untuk mengetahui perbedaan perlakuan yang diberikan. Hasil penelitian menunjukan bahwa pemberian sopi 5.4 ml dapat menyebabkan penurunan jumlah sel Leydig dan sel Sertoli dan setelah diberi ekstrak etanol sirih cina, jumlah sel Leydig dan sel Sertoli mengalami peningkatan, dimana dosis ekstrak etanol sirih cina yang baik untuk sel Leydig adalah 2.86g dan untuk sel Sertoli adalah dosis 1.43g.
Phytotherapy for Diabetes: A Preclinical Study of Ethanol Extract of Winged Bean (Psophocarpus tetragonolobus L.) Leaves in Diabetic Rat (Rattus norvegicus) Models Moniharapon, Mechiavel; Tahapary, Patrcik Indurian; Kaihena, Martha; Eddy, La
Biofaal Journal Vol 6 No 2 (2025): Biofaal Journal
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/biofaal.v6i2pp125-140

Abstract

The long-term use of synthetic hypoglycemic agents is known to cause adverse side effects and induce drug resistance. Winged bean (Psophocarpus tetragonolobus L.), a traditional medicinal plant from Maluku, contains secondary metabolites such as flavonoids, saponins, and tannins, which are believed to have potential in lowering blood glucose levels. This study aimed to investigate the effect of ethanolic extract of winged bean leaves on blood glucose reduction and β-cell regeneration in the pancreas of white rats (Rattus norvegicus) as a diabetic model. Thirty rats weighing between 150–200 g were divided into five groups: Group I (negative control), Group II (positive control), and Groups III, IV, and V (diabetic rats treated with ethanolic extract of winged bean leaves at doses of 2.52 mg/g BW, 5.04 mg/g BW, and 7.56 mg/g BW, respectively). Streptozotocin (STZ) was administered to Groups II–V to induce diabetes. Rats with blood glucose levels exceeding 200 mg/dL were subsequently treated with the designated doses of the extract in Groups III–V. On the final day, blood glucose levels were measured, and pancreatic organs were harvested for histological analysis. The results demonstrated that the ethanolic extract of winged bean leaves at a dose of 7.56 mg/g BW effectively reduced blood glucose levels in diabetic rats.
Co-Authors Adrien Jems Akiles Unitly Amos Killay Baszary, Chomsa Dintasari Umi Efraim Samson, Efraim Fredy Pattipeilohy Heryanus Jesajas Kaliky, Nunun Ainun Putri Sari Banun Kiriweno, Grasia Welma La Eddy La Eddy Lakesubun, Bella Frida LAURY CHARA HUWAE Lesbatta, Kezia Josawel Lilian M Soukotta Lokollo, Richard R. Martha Kaihena Meigy N Mailoa Moniharapon, Debby D. Moniharapon, Debby Dijola Muskita, Edwin Nadira Pattimura Pangemanan, Victory O Pieter Kakisina Restu Tuharea Riandini, Rezki Amalia Rony Kunda Rony Marsyal Kunda Sahlan Hamid Saman, Risna Sanita Suriani Sikafir, Beatrix Belina Silahooy, Veince B Silahooy, Veince Benjamin Sillehu, Eka Safitri Sopacua, Gracia N. Tahapary, Patrcik Indurian Theopilus W Watuguly Trijunianto Moniharapon Tuasella, Frisca N Ukratalo, Abdul M Utami, Prasetyarti Veince Benyamin Silahooy, Veince Benyamin