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All Journal Rekayasa: Jurnal Penerapan Teknologi dan Pembelajaran Indonesian Journal of Cancer Chemoprevention Jurnal Psikologi Indonesian Journal of Biotechnology Indonesian Journal of Chemical Science Knowledge Engineering and Data Science Jurnal Eurekamatika Pattimura Proceeding : Conference of Science and Technology International Journal of Cell and Biomedical Science Biosaintifika: Journal of Biology & Biology Education
Amalina, Nur Dina
Pharmacy Study Program, Faculty Of Mathematics And Natural Sciences, Semarang State University, Semarang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Energy Engineering Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Physics Social Sciences

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BUKTI ALTERNATIF INTERPOLASI KOMPLEKS RUANG LEBESGUE DENGAN EKSPONEN PEUBAH Dina Nur Amalina; Denny Ivanal Hakim
Pattimura Proceeding 2021: Prosiding KNM XX
Publisher : Pattimura University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1044.705 KB) | DOI: 10.30598/PattimuraSci.2021.KNMXX.61-66

Abstract

Pharmacokinetics Profile of Chitosan Nanoparticles in Chronic Lead-induced Toxicity Rats Model Marianti, Aditya; Amalina, Nur Dina; Mursiti, Sri; Sitompul, Faya Nuralda; Futri, Shafira Septiana; Negara, Legendra Gantar; Sholehah, Intan Kharyna; Asmorowati, Dian Sri; Astari, Putri Dyah
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 1 (2024): April 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v15i1.1857

Abstract

Chronic lead exposure induces ROS accumulation which causes physiological disorders. Chelation therapy has been widely used to overcome lead poisoning since it exerts only a few side effects. Nano chitosan prevents lead poisoning by inhibiting ROS. This study examined the pharmacokinetics of nano chitosan in chronic lead-induced toxicity animal models and the mechanism of action pathway using the bioinformatic approach, The area under the curve was estimated to be 12110.13 ± 7709.37 μg/mL hours using the pharmacokinetic model, and the Cmax was 82.34 ± 5.64 μg/mL. The Tmax and t½ calculations were 22.68 ± 11.67 and 80.47 ± 60.58 hours respectively. Chitosan nanoparticles regulated VEGFA, FGF2, and LGALS3 which plausibly played a substantial role in chronic lead exposure. However, chitosan is not suitable for oral administration due to its low gastrointestinal solubility. These characteristics make chitosan nanoparticles have the prospect of being developed as a supplement so that they can contribute to overcoming the negative impacts of chronic lead poisoning.
Therapeutic Potential of Secretome Hypoxia Mesenchymal Stem Cells: Downregulation of TNF-α and HIF-2α in Metabolic Syndrome-Induced Inflammation in Wistar Rats Dewi, Alisia Martha; Trisnasi, Setyo; Putra, Agung; Chodijah , Chodijah; Sarosa, Hadi; Mulyani, Sri Priyantini; Amalina, Nur Dina; Ibrahim, Sugeng
Biosaintifika: Journal of Biology & Biology Education Vol. 16 No. 2 (2024): August 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v16i2.6613

Abstract

Background: Metabolic syndrome (MetS) has become a global health challenge with several associated issues, such as obesity, insulin resistance, dyslipidemia, and hypertension. Important proteins such as Tumor Necrosis Factor-alpha (TNF-α) and Hypoxia Inducible Factor-2 alpha (HIF-2α) regulate the inflammatory process by inducing the expression of pro-inflammatory proteins. Secretome Hypoxia Mesenchymal Stem Cells (SH-MSCs) are immunomodulatory, anti-inflammatory, and angiogenic stimulators, which can regulate various inflammatory diseases, including MetS. Objective: This study aims to determine the effect of administering SH-MSCSs on the expression of the TNF-α and Hypoxia Inducible Factor (HIF)-2α genes in the male Wistar rat model with Metabolic Syndrome. Method: This research is an experimental study with a Post-test Only Control Group Design, using a total of 24 male Wistar rats divided into four groups: T1 (Healthy control), T2 (MetS + NaCl), T3 (MetS + administration of SH-MSCs dose 150 uL), and T4 (MetS + administration of SH-MSCs dose 300 uL). SH-MSCSs were administered intraperitoneally four times over 14 days. Adipose tissue TNF-α and HIF-2α gene expression were measured on day 15 using qRT-PCR. Results: TNF-α and HIF-2α gene expression was significantly lower in T3 and T4, compared with the MetS control group (T2). Conclusion: Administration of SH-MSCs was able to reduce the expression of the Tumor Necrosis Factor (TNF-α) and Hypoxia Inducible Factor (HIF)-2α genes in fatty tissue in the male Wistar rat model with Metabolic Syndrome.
Application of Bioinformatics Analysis to Identify Important Pathways and Hub Genes in Breast Cancer Affected by HER-2 Tjipta, Arya; Hermansyah, Dedy; Suzery, Meiny; Cahyono, Bambang; Amalina, Nur Dina
International Journal of Cell and Biomedical Science Vol 1 No 1 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i1.11

Abstract

Human epidermal growth factor receptor 2 (HER-2) is used as a marker for the diagnosis and prognosis of breast cancer. However, the molecular mechanisms involving HER2 in breast cancer require further study. Herein, we used the bioinformatics approaches to identify important pathways and hub genes in breast cancer affected by HER-2. The results showed that HER-2 is highly expressed in ovarian cancer and is closely related to the overall survival and progression-free survival of breast cancer. A total of 3014 downregulated genes and 4121 upregulated genes were identified under Gene Expression Omnibus (GEO) database with the GEO2R tool. Among them, the top 10 hub genes including CCNB1, KIF11, BUB1B, TOP2A, ASPM, MAD2L1, BUB1, RRM2, EGFR, and FN1 demonstrated by connectivity degree in the protein-protein interaction (PPI) network were screened out. In Kaplan–Meier plotter survival analysis, the overexpression of CCNB1, EGFR, MAD2L1, ASPM, and RRM2 were shown to be associated with an unfavourable prognosis in HER-2 positive breast cancer patients. In conclusion, we have identified important signalling pathways involving HER-2 that affect breast cancer. These findings could provide new insights outlining mechanisms involving HER-2 gene expression in breast cancer and provides a rationale for the novel treatment of breast cancer.
The Potential of Moringa Leaf Extract to Prevent Aging Targeted Cellular Senescence Sri Andriyani; Rujitoningtyas, Karimatussholikah; Wigati, Hilmi U.; Amalina, Nur Dina
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.21

Abstract

High exposure to ultraviolet light and chemicals causes cell senescence due to the overproduction of reactive oxygen species (ROS). Cell senescence is known as one of the causes of degenerative diseases. Reducing ROS levels using antioxidant compounds can overcome cell senescence. This study aimed to evaluate the potential of Moringa leaf extract (EDK) as an anti-aging agent and predict the molecular mechanisms underlying its activity. EDK active compounds were identified through qualitative phytochemical screening, a cytotoxic assay was evaluate using MTT assay, ROS level calculate under DCFDA flow cytometry assay, and cell senescence analyze using SA-β-galactosidase assay. The molecular mechanism that plays an important role in senescence was analyzed through bioinformatics studies and molecular docking in silico to investigate the interaction strength of the active compound EDK with the target protein. The results showed that the EDK ethanol extract contained flavonoid, phenolic, alkaloid, saponin, and steroid compounds. This extract did not give any cytotoxic effect on NIH3T3 cells, as evidenced by the IC50 value of 420μg/mL. EDK concentrations of ¼IC50 (105μg/mL) and ½IC50 (210μg/mL) significantly reduced ROS level in dose-dependent manner in the NIH3T3 cell population with high oxidative stress induced by Doxorubicin 10nM (Dox). The percentage of senescence cells also decreased due to EDK 210μg/mL administration up to 47.60% compared to the positive aging control Dox 10nM (78.90%). Bioinformatics studies found that p21 and TP53 proteins were most directly affected by EDK active compounds in the senescence pathway. The quercetin compound in EDK has the most robust interaction strength against p21 and TP53 proteins compared to ligands. In conclusion, it can be concluded that the active compound EDK can suppress intracellular ROS levels and compete with p21 and TP53 proteins in preventing cell senescence.
The Effect of Hypoxic Mesenchymal Cell Secretome Administration on VEGF Levels In Type 1 Diabetes Rats Model Jumena, Finanda; Amalina, Nur Dina; Dewi Antari, Arini
International Journal of Cell and Biomedical Science Vol 1 No 3 (2022)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v1i3.22

Abstract

Type 1 diabetes mellitus (T1DM) is an autoimmune disease characterised by the cessation of insulin production due to pancreatic β-cell damage resulting in an increase in blood glucose. This study aims to analyse the effect of hypoxic secretome MSCs on the angiogenesis process through the observation of VEGF levels in T1DM rats model. The twenty animal model were randomly assigned to four groups: control T1DM, T1DM with HS-MSCs 0.5 mL intraperitoneal treatment (T1), and TIDM with hypoxic secretom mesenchymal stem cells (HS-MSCs) 1 mL intraperitoneal treatment (T2). The T1DM rats model was induced by a single intraperitoneal (IP) injection of freshly prepared streptozotocin (STZ) at a dose of 65 mg/kg of body weight. The VEGF levels was analyses under ELISA assay. The results showed that VEGF levels of T1 (68.86±4.78) and T2 (53.83±10.86) groups were significantly upregulated in treatment of HS-MSCs. Taken together, HS-MSCs potentially reduce glucose levels on T1DM through VEGF up-regulation.
Clitoria ternatea Flower Extract Inhibits Matrix Mettaloproteinase-3 leading to the promotion of α-SMA Gene Expression in UVB-Induced Rat Model Muna, Nugraheni Itsnal; Amalina, Nur Dina; Husain, Sofian Azalia
International Journal of Cell and Biomedical Science Vol 2 No 5 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i5.33

Abstract

This study aimed to determine the effect of administration of Clitorea ternatea flower extract (C. ternatea) in a gel on the expression of MMP-3 and α-SMA in the skin of rat models induced by UVB light exposure. This research design is experimental research with a post-test control group design. This study was divided into four treatment groups: a healthy group, a UVB-negative control, a 5% C. ternatea gel treatment group (T1), and a 10% C. ternatea gel treatment group (T2). Negative control, T1, and T2 control groups were given UVB treatment with 302 nm energy of 160 mJ/cm2 for 5 days. In the treatment groups T1 and T2 were given the gel of C. ternatea from the 6th day until the 14th day. On day 15, all treatment groups were terminated, and the expression of MMP-3 and α-SMA genes was analyzed using qRT-PCR. In the 10% C. ternatea gel treatment group, there was a decrease in MMP-3 gene expression (0.73±0.26) and an increase in α-SMA gene expression (16.4±8.3) compared to the UVB control group which had gene expression. MMP-3 (3.24+1.05) and α-SMA gene expression (0.1±0.0). The administration of C. ternatea gel resulted in a reduction in the expression of the MMP-3 gene and an increase in the expression of the α-SMA gene. Overall, C. ternatea has the potential to prevent UVB-induced hyperpigmentation.
Anti-inflammation effect of Apium graveolens Extract against lead-acetate-induced brain injury in rats Sulistyo, Sona; Sarosa, Hadi; Sumarawati, Titiek; Putra, Agung; Chodidjah, Chodidjah; Amalina, Nur Dina; Ibrahim, Sugeng
International Journal of Cell and Biomedical Science Vol 2 No 6 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i6.41

Abstract

Purpose: The current study investigated the protective potential of Apium graveolens extract (APE) against lead-induced brain injury in rats by exploring anti-inflammatory and antiapoptotic mechanism. Methods: Twenty male Wistar rats were randomly allocated into four groups (n=5). The control group was orally administrated with distillate water. The second group received lead acetate 200mg/kg body weight orally for 14 days, the third group were orally administered lead acetate 200 mg lead acetate/kg body weight and vitamin E 50IU/kg body weight for 14 days. The fourth group was administrated with leas acetate like second group and APE 300mg/kg body weight for 14 days. The TNF-a levels and caspase-3 expression was analyses under ELISA and flow cytometry assay, respectively. Results: The phytochemical analysis of APE indicated the presence of alkaloids, flavonoids, tannins, saponins, and steroids. Leads acetate increased the serum levels of TNF-α and caspase-3 expression, as well as altering the brain tissue architecture. Conclusion: In conclusion, the presence of APE inhibited the lead acetate toxicity by inhibition of TNF-α proinflammation protein and caspase-3 proapoptosis protein.
The Effect of Celery Extract on Caspase-3 and TNF-α Gene Expression in Lead Poisoning-Induced Renal Injury in Rats Purwaningsih, Hesti; Sumarawati, Titiek; Chodidjah, Chodidjah; Putra, Agung; Priyantini, Sri; Fasitasari, Minidian; Ibrahim, Sugeng; Amalina, Nur Dina
International Journal of Cell and Biomedical Science Vol 2 No 6 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i6.42

Abstract

Background To determine the effect of Apium graveolens L (Celery) extract in the TNF-α and caspase-3 gene expression on the lead poisoning-induced renal injury rats’ model. Methods This study is experimental research with post test only control group design. Twenty male Wistar rats were randomly allocated into four groups (n=5). The control group was orally administrated with distillate water. The second group received lead acetate 200mg/kg body weight orally for 14 days, the third group were orally administered lead acetate 200 mg lead acetate/kg body weight and vitamin E 50IU/kg body weight for 14 days. The fourth group was administrated with leas acetate like second group and celery extract 300mg/kg body weight for 14 days. The TNF-a and caspase-3 gene expression was analyses under qRT-PCR. Results The phytochemical analysis of APE indicated the presence of alkaloids, flavonoids, tannins, saponins, and steroids. Leads acetate increased the TNF-α (3.87±0.09) and caspase-3 (7.95±0.23) gene expression. The celery extract was significantly decrease in the TNF-α (3.13±0.34) and caspase-3 (2.48±1.23) gene expression. Conclusion: In conclusion, the presence of celery extract inhibited the renal injury-induced lead acetate toxicity by inhibition of TNF-α proinflammation protein and caspase-3 proapoptosis protein
Inhibitory Effects of Petai Peel Extract Gel on Tyrosinase and TRP1 Gene Expression in UVB-Exposed Mouse Skin Hutabarat, Nenny Lynda Caroline; Subchan, Prastyowati; Putra, Agung; Amalina, Nur Dina; Sitompul, Faya Nuralda
International Journal of Cell and Biomedical Science Vol 2 No 6 (2023)
Publisher : Stem Cell and Cancer Research (SCCR)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59278/cbs.v2i6.43

Abstract

Background: UVB irradiation can induce the formation of Reactive Oxygen Species (ROS), which causes the activation of melanin synthesis through the activation of tyrosinase and tyrosinase-related protein-1 (TRP1). Secondary metabolites in stink bean peel extract inhibit ROS production due to exposure to UVB rays. This study aims to determine the effect of administering stink bean peel extract gel on the expression of the tyrosinase and TRp1 genes in mouse skin tissue exposed to UVB. Method: The research design was a posttest-only control group with a completely randomized design method. The samples studied were 24 mice exposed to UVB light with a wavelength of 302 nm and an energy of 390mJ/cm2/day 3 times a week for 2 weeks. This research was carried out in four groups: the healthy group, the negative control group, treatment 1 (T1) with 10% stink bean peel extract gel, and treatment 2 (T2) with 20% stink bean peel extract gel. Tyrosinase and TRP1 gene expression were analyzed using qRT-PCR. Results: qRT-PCR analysis showed that there was a significant decrease in tyrosinase and TRP1 gene expression between groups T1 (tyrosinase 3,19±2,12 and TRP1 4,96±3,42) and T2 (tyrosinase 0,65±0,44 and TRP1 2,22±1,18) compared to negative control (tyrosinase 17,92±3,77 and TRP1 35,91±4,52). Conclusion: The administration of stink bean peel extract gel has shown promising results in reducing the expression of tyrosinase and TRP1 genes in hyperpigmentation mice exposed to UVB light. This suggests that stink bean peel extract could be a safe and effective therapeutic approach for preventing UVB-induced hyperpigmentation.
Co-Authors Achmad Fauzan, Achmad Aditya Marianti Agung Putra Agung Putra Amalia, Najla Amansyah, Fajar Astari, Putri Dyah Bambang Cahyono Bayu Tirta Dirja Chodidjah Chodidjah Chodijah , Chodijah Damar Nurwahyu Bima Dedy Hermansyah Denny Ivanal Hakim Dewi Antari, Arini Dewi, Alisia Martha Dian Sri Asmorowati Edy Meiyanto Encum Sumiaty, Encum Endang Susilaningsih Futri, Shafira Septiana Hadi Sarosa Harjito - Husain, Sofian Azalia Hutabarat, Nenny Lynda Caroline Ibrahim, Sugeng Ika Putri Nurhayati Jumena, Finanda Kurniasari, Laviona Tiara Kusumo, M. Hidayat Budi Marpaung, Adelia Septianingrum Masta, Al Azhary Meiny Suzery Minidian Fasitasari Muhammad Abdan Shadiqi Muna, Nugraheni Itsnal Murbangun Nuswowati Nanik Wijayati Negara, Legendra Gantar Pangesti Drawina Purwaningsih, Hesti Rujitoningtyas, Karimatussholikah S Mursiti Safitri, Nadiya Nur Salbiyah, Siti Sholehah, Intan Kharyna Sitompul, Faya Nuralda Sri Andriyani Sri Kadarwati Sri Priyantini Sri Priyantini Mulyani Subchan, Prastyowati Sulistyo, Sona Taufiqurrachman Nasihun Titiek Sumarawati Tjipta, Arya Trisnasi, Setyo Wigati, Hilmi U. Yan Wisnu Prajoko