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Uji Efektivitas Ekstrak Etanol Daun Mahoni (Swietenia mahagoni L.) Dalam Sediaan Losion Sebagai Repelan Terhadap Nyamuk Aedes aegypti Rosari, Anastasia Santaulina Putri; Setyaningrum, Endah; Pratami, Gina Dania; Widiastuti, Endang Linirin
JURNAL BIOS LOGOS Vol. 14 No. 3 (2024): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.v14i3.56344

Preventive efforts made to avoid mosquito bites generally use repellants containing synthetic chemicals in the form of N, N-diethyl-meta-toluamide (DEET) and if used for a long period of time can cause damage to the human body. It is known that mahogany plants (Swietenia mahagoni L.) contain secondary metabolite compounds that have the potential as insect repellants. This study aims to determine the effectiveness of ethanol extract of mahogany leaves (S. mahagoni L.) in lotion as a mosquito repellent for Ae. aegypti, Effective Concentration 50% (EC50), physical properties and irritating effects of mahogany leaf ethanol extract lotion. This research was conducted in January 2024 - March 2024 at the Botany Laboratory and Zoology Laboratory, FMIPA, Unila. The study used a completely randomized design (CRD) with 4 treatments and 6 repetitions consisting of 4 levels of test concentrations, namely 0%, 5%, 10%, and 15%. The results of this study showed that the lotion of ethanol extract of mahogany leaves at a concentration of 15% had a protective power as a repellant of 87.63%, the EC50 value of the extract lotion at a concentration of 9,60%. The lotion has good physical properties and does not cause irritation on the skin. In conclusion, ethanol extract of mahogany leaves has effectiveness as a repellent for Aedes aegypti mosquitoes.

Empowering coastal women in improving family economy in Margasari Utomo, Agus Budi; Rochana, Erna; Widiastuti, Endang Linirin; Hartoyo, Hartoyo; Riantini, Maya
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 9, No 1 (2025)
Publisher : Sangia Research Media and Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.9.1.33-40

This study aims to see the empowerment efforts of coastal women in improving the family economy in Margasari Village, Labuhan Maringgai District, East Lampung Regency. This study used a qualitative research type with an ethnographic approach. The focus of the study was on four Poklahsar in Margasari Village. Data collection techniques were carried out through observation, interviews, and documentation studies. Then, qualitative data analysis was carried out with the stages of data reduction, data presentation, and data collection. The results of the study are that the empowerment of coastal women in Margasari Village through Poklahsar is carried out by providing various training, workshops, mentoring and assistance, both capital and production support facilities. Although the empowerment carried out has been quite massive, the impact of increasing income for families is still not significant because the marketing of processed food products produced by Poklahsar has not been optimal.

Potensi Antikanker Pada Ekstrak Etanol Makroalga Caulerpa racemosa Menggunakan Metode Brine Shrimp Lethality Test (BSLT) Khasana, Devi Ardiyati; Widiastuti, Endang Linirin; Nurcahyani, Nuning; Setyawan, Agus; Juliasih, Ni Luh Gede Ratna
Journal of Tropical Marine Science Vol 8 No 1 (2025): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v8i1.5759

Caulerpa racemosa is one of the green macroalgae that is widely distributed in the coastal areas of Lampung Province and needs to be explored as a phytopharmaceutical ingredient because it is thought to have bioactive compounds. In this study, we also used taurine, which is known to prevent cell and tissue damage due to oxidative processes, to explore its potential as an anticancer. The initial stage of this research is phytochemical screening of 96% ethanol extract of Caulerpa racemosa and contains phenolic compounds . alkaloids, saponins, flavonoids, tannins, steroids and terpenoids. The toxicity test of Caulerpa racemosa ethanol extract and taurine used the BSLT method (Brine Shrimp Lethality Test). In this study, 7 concentrations were used, namely 0 ppm (control); 62.5; 125; 250; 500; 1000; and 2000 for both Caulerpa racemosa and taurine ethanol extracts with 5 repetitions. Meanwhile, the toxicity test results showed that the LC50 value of Caulerpa racemosa ethanol extract was 194.14 ppm and 170.69 ppm for taurine, this values are between 100 ppm - 1000 ppm of Caulerpa racemosa ethanol and taurine indicate that both material have potential anticancer activity with low toxicity.

Empowering coastal women in improving family economy in Margasari Utomo, Agus Budi; Rochana, Erna; Widiastuti, Endang Linirin; Hartoyo, Hartoyo; Riantini, Maya
Akuatikisle: Jurnal Akuakultur, Pesisir dan Pulau-Pulau Kecil Vol 9, No 1 (2025)
Publisher : Sangia Research Media and Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29239/j.akuatikisle.9.1.33-40

This study aims to see the empowerment efforts of coastal women in improving the family economy in Margasari Village, Labuhan Maringgai District, East Lampung Regency. This study used a qualitative research type with an ethnographic approach. The focus of the study was on four Poklahsar in Margasari Village. Data collection techniques were carried out through observation, interviews, and documentation studies. Then, qualitative data analysis was carried out with the stages of data reduction, data presentation, and data collection. The results of the study are that the empowerment of coastal women in Margasari Village through Poklahsar is carried out by providing various training, workshops, mentoring and assistance, both capital and production support facilities. Although the empowerment carried out has been quite massive, the impact of increasing income for families is still not significant because the marketing of processed food products produced by Poklahsar has not been optimal.

ANTIOXIDANT POTENTIAL OF BIOACTIVE COMPOUNDS IN ETHANOL EXTRACTS OF SEAGRASS AND MACROALGAE FROM LAMPUNG WATERS Bareta, Ainun Rohmawati; Widiastuti, Endang Linirin; Nurcahyani, Nuning; Roiska, Riris; Leni, Yusyam
Jurnal Perikanan Unram Vol 15 No 1 (2025): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v15i1.1418

The sea in Indonesia covers 75% of its total land area. According to data from the Ministry of Marine Affairs and Fisheries (2019), Indonesia has approximately 5.8 million km² of water area out of a total territory of 7.81 million km². The biodiversity in Indonesia's coastal areas is utilized by coastal communities in their daily lives. Coastal resources also have potential in the health sector, such as seagrass, macroalgae, and taurine, which can be used as raw materials for natural medicine. The purpose of this study is to analyze the antioxidant potential based on the IC50 value of taurine, ethanol extracts of the seagrasses Enhalus acoroides and Cymodocea rotundata, as well as the macroalgae Padina australis and Sargassum duplicatum. The method used is the antioxidant activity assay using DPPH (2,2-Diphenyl-1-picrylhydrazyl). The results of this study indicate that, after testing with DPPH, only the ethanol extract of Cymodocea rotundata had an IC50 value of < 200 µg/mL, specifically -652.95 µg/mL, whereas the IC50 values of taurine, the ethanol extract of Enhalus acoroides, and the ethanol extracts of the macroalgae Padina australis and Sargassum duplicatum were > 200 µg/mL, indicating no antioxidant activity. In conclusion, only the ethanol extract of Cymodocea rotundata has the potential to be a candidate for antioxidant raw material.

Microplastic Analysis at Sea Water and Sediment in the Mahitam Island Lampung Bay using FT-IR Nita Sari; Widiastuti, Endang Linirin; Pratami, Gina Dania; Tugiyono, Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.255

The amount of garbage that accumulates in the waters causes environmental damage which is getting worse. The breakdown of plastic waste into smaller particles that have been degraded is called microplastic. This study aims to analyze the number of particles, shapes, and types of microplastic polymers found in the water and sediments of Mahitam Island. The research location was determined by purposive random sampling with three stations based on plastic sources, namely Station I, Station II, and Station III, which had different characteristics from laboratory-based testing for seawater and sediment. Microplastic analysis on seawater samples was carried out by adding a solution of 70% ethanol, 30% H2O2, and 30% NaCl, and on sediment samples was carried out by adding a solution of FeSO4 (0.05 M), NaCl, and 30% H2O2. The content of the number of particles and the shape of microplastics were analyzed using a microscope, while the analysis of microplastic polymers using FT-IR. The first thing to do is to extract microplastics The forms of microplastic found are Fiber, Film, Fragments, and Pellets. The highest number of microplastic particles was the type of film for water samples at Station 1 and Station 2 for sediment samples. The types of polymers found in water and sediments on Mahitam Island are polyvinyl chloride (PVC), polyethylene (PE), polypropylene (PP), and polystyrene (PS) which come from plastic waste. Microplastics are known to originate from the use of plastic originating from tourist activities and originating from the sea currents of Mahitam Island.

Performa Instalasi Pengolah Air Limbah (IPAL) Sederhana Tambak Udang Vaname di Desa Ruguk, Kecamatan Ketapang, Kabupaten Lampung Selatan Lestari, Sepnina Like; Hasani, Qadar; Setyawan, Agus; Tugiyono, Tugiyono; Susanto, Gregorius Nugroho; Widiastuti, Endang Linirin; Arlanda, Revilarita; Khasanah, Nurul; Supono, Supono
Akuatiklestari Vol 8 No 2 (2025): Jurnal Akuatiklestari
Publisher : Program Studi Manajemen Sumberdaya Perairan, Fakultas Ilmu Kelautan dan Perikanan, Universitas Maritim Raja Ali Haji

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31629/akuatiklestari.v8i2.7112

Intensifikasi budidaya udang vaname (Litopenaeus vannamei) tidak hanya meningkatkan produksi udang tetapi juga berdampak pada penurunan kualitas air buangan dari tambak udang ke perairan pantai. Pengadaan instalasi pengolahan air limbah pada outlet mbah budidaya udang menjadikan salah satu pilihan untuk mereduksi bahan pencemar. Tujuan penelitian ini untuk mengevaluasi IPAL dalam mereduksi kandungan fosfat, nitrat, total ammonia nitrogen (TAN), total organik matter (TOM) di tambak udang vaname Desa Ruguk, Kecamatan Ketapang, Kabupaten Lampung Selatan. Sebanyak dua kolam digunakan sebagai IPAL (IPAL1 dan IPAL2) dengan luas kolam masing-masing 2.054 m2 dari 12 kolam tambak udang vaname. Keberadaan IPAL di tambak dievaluasi dengan pengukuran kualitas air di empat stasiun yaitu outlet, kolam IPAL 1, kolam IPAL 2, dan main outlet atau aliran Sungai sebanyak tiga kali (Sebelum panen, panen parsial, dan panen total). Parameter kimia yang diukur pada penelitian ini yaitu yaitu pH, nitrat, fosfat, total ammonia nitrogen (TAN), alkalinitas, oksigen terlarut. Serta, mengamati pertembuhan parameter biologi yaitu total Vibrio count (TVC). Hasil penelitian menunjukkan bahwa efektivitas IPAL sederhana tidak efisien menurunkan nitrat (9%), pH (3%), alkalinitas (12%). Sedangkan salinitas (31%), DO (41%), fosfat (55%), dan TAN (61%), termasuk cukup efisien. Kelimpahan bakteri Vibrio meningkat 95% menuju day of care DOC 134 (panen total).

Potensi Antikanker Pada Ekstrak Etanol Makroalga Caulerpa racemosa Menggunakan Metode Brine Shrimp Lethality Test (BSLT) Khasana, Devi Ardiyati; Widiastuti, Endang Linirin; Nurcahyani, Nuning; Setyawan, Agus; Juliasih, Ni Luh Gede Ratna
Journal of Tropical Marine Science Vol 8 No 1 (2025): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v8i1.5759

Caulerpa racemosa is one of the green macroalgae that is widely distributed in the coastal areas of Lampung Province and needs to be explored as a phytopharmaceutical ingredient because it is thought to have bioactive compounds. In this study, we also used taurine, which is known to prevent cell and tissue damage due to oxidative processes, to explore its potential as an anticancer. The initial stage of this research is phytochemical screening of 96% ethanol extract of Caulerpa racemosa and contains phenolic compounds . alkaloids, saponins, flavonoids, tannins, steroids and terpenoids. The toxicity test of Caulerpa racemosa ethanol extract and taurine used the BSLT method (Brine Shrimp Lethality Test). In this study, 7 concentrations were used, namely 0 ppm (control); 62.5; 125; 250; 500; 1000; and 2000 for both Caulerpa racemosa and taurine ethanol extracts with 5 repetitions. Meanwhile, the toxicity test results showed that the LC50 value of Caulerpa racemosa ethanol extract was 194.14 ppm and 170.69 ppm for taurine, this values are between 100 ppm - 1000 ppm of Caulerpa racemosa ethanol and taurine indicate that both material have potential anticancer activity with low toxicity.

Prevalence and Patterns of Pneumonia in Histopathology of Lungs from Qurban Cattle (Bos spp.) in Lampung, Indonesia (2019): A Laboratory-Based Survey Nursafitri, Umy; Sutyarso; Susanto, Gregorius Nurgroho; Widiastuti, Endang Linirin
ISEJ : Indonesian Science Education Journal Vol. 5 No. 1 (2024): January
Publisher : Yayasan Darussalam Bengkulu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62159/isej.v5i1.1752

Pneumonia in cattle compromises welfare and carcass value, yet abattoir-based histopathology evidence from Indonesia’s qurban context remains scarce. To estimate the prevalence of pneumonia and characterize lesion patterns in lungs from qurban cattle processed in Lampung Province (2019). A cross-sectional, laboratory study analyzed 20 bovine lungs collected opportunistically from four districts (Bandar Lampung, Lampung Timur, Pringsewu, Metro). Representative tissues were fixed in 10% neutral buffered formalin, paraffin-embedded, sectioned at 4–5 µm, and stained with hematoxylin–eosin; bright-field microscopy (≈40×–400×) classified lesions a priori as interstitial pneumonia, bronchopneumonia, or aspiration-consistent pneumonia, and annotated severity (mild/moderate), temporality (acute/chronic), and distribution (focal/multifocal). Analyses were descriptive. 13/20 (65%) lungs met histological criteria for pneumonia, while 7/20 were normal. Interstitial cases showed alveolar-septal thickening, congestion/hemorrhage, and alveolar edema; bronchopneumonia was airway-centered with peribronchiolar cuffing and suppurative/fibrinosuppurative exudates; aspiration-consistent lesions exhibited focal/multifocal cranioventral involvement with foreign particulate material and neutrophilic exudates, occasionally with multinucleated giant cells. Most lesions were mild–moderate and predominantly acute. The profile indicates a multifactorial aetiology in which upstream infectious processes and perimortem handling both contribute, underscoring the added value of histopathology over gross inspection alone. Findings support immediate refinement of slaughter procedures (restraint, incision sequence, head positioning) to reduce aspiration risk; routine integration of histopathology paired with culture/PCR into abattoir surveillance for actionable supplier feedback; and periodic, micrograph-based auditing and training to strengthen food safety, animal welfare, and biosecurity.

Pelatihan Pembuatan Minuman Herbal untuk Immune Booster Staff Kebun Raya Liwa Di Masa Pandemi Covid-19 Setyaningrum, Endah; Widiastuti, Endang Linirin; Arifiyanto, Achmad
Hippocampus: Jurnal Pengabdian Kepada Masyarakat Vol 1 No 2 (2022): December 2022
Publisher : PUSAT PENELITIAN DAN PENGABDIAN PADA MASYARAKAT (PPPM) POLITEKNIK NEGERI SAMBAS

The Sars-Cov2 virus, also known as the Covid-19 pandemic, has led in a rise in the usage of immune-boosting supplement items. This situation resulted in higher prices and a scarcity of stock on the market. This program seeks to motivate and educate UPT Liwa Botanical Garden (KRL) employees about the necessity of utilizing herbs to boost body ’s immune system during the Covid-19 outbreak. The activity's target audience is workers, who interact often with tourists from various locations. Twenty persons participated, including KRL officials, Lumbok Seminung Kehati Park Managers, and KRL apprentice students. This activity takes the form of providing content, question and answer sessions, demonstrations on how to make herbal beverages, and training assessment using pre and post-test questionnaires. The results of the training implementation revealed an increase in participants' motivation and understanding of the importance of herbal functions and the skills of making herbal drinks, as evidenced by their enthusiasm in the discussion both before and after the demonstration of making herbal drinks.

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