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Tugiyono Tugiyono
Industrial Engineering Undergraduate Program Muhammadiyah Technology College of Kebumen Pahlawan St. No.188 Mertokondo Kebumen Post code 54317
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Nursing Physics Public Health Social Sciences Other

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Plankton Community Structure as a Bioindicator of Water Quality in the Way Sekampung River- the Segment of Rulung Helok Tugiyono, Tugiyono; Ayuni, Ubaid Jan; Priyambodo, Priyambodo; Susanto, Gregorius Nugroho
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 2 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i2.293

Abstract

Planktons are microorganisms that live in aquatic habitats. Plankton is used as a quality bioindicator of a water body. Way Sekampung flows to several areas in Lampung Province. The existence of activities around the watershed such as settlements, agriculture, and industrial waste disposal is thought to affect the water quality of the Way Sekampung River. The purpose of this study was to determine the water quality of Way Sekampung River based on plankton community structure consisting of abundance index, diversity index, uniformity index, and dominance index. Water sample data were analyzed using chemical parameters namely pH, BOD, DO, Phosphate, Nitrate, and physical parameters namely temperature and Total Suspended Solid (TSS) which are used to determine the status of water quality standards. This research was conducted at the beginning of the dry season (April) and the beginning of the rainy season (October). Sampling used a survey method based on the Way Sekampung River segment. Sampling points were carried out before and after the Argoguruh Dam. The results showed that the water quality of Way Sekampung River at the beginning of the dry season (April) and the beginning of the rainy season (October) were moderately polluted based on the analysis of plankton community structure such as abundance index, diversity, uniformity, and dominance.
Differences in Soaking Time of Sea Urchin Gonad Extract (Diadema setosum) on Nile Tilapia (Oreochromis niloticus) Masculinization with a Dose of 4 mg/L : Perbedaan Lama Perendaman Ekstrak Gonad Bulu Babi (Diadema Setosum) Terhadap Maskulinisasi Ikan Nila (Oreochromis Niloticus) Dengan Dosis 4 Mg/L Sari, Mutiara Pradita; Susanto, Gregorius Nugroho; Widiastuti, Endang Linirin; Tugiyono, Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 2 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i2.310

Abstract

Nile tilapia (Oreochromis niloticus) is a fish that is in demand because of its high economic value and thick meat. Tilapia is easy to breed, uncontrolled spawning can occur so that growth is inhibited. The growth of male tilapia is faster than females at the same age. To increase the growth rate, sex reversal is carried out towards males through immersion in steroid hormones. Bioactive substances contained in sea urchins are steroid compounds. The purpose of this study is to determine the effect of the length of immersion in sea urchin gonad extract (Diadema setosum) on the formation of male phenotypes of tilapia larvae and tilapia survival. The method used is a completely randomized design with 4 treatments and each treatment with 3 replicates at a dose of 4 mg/L with soaking times of 0, 12, 18, and 24 hours. Data are analyzed using SPSS 16 software with analysis of variance (One-way ANOVA). The results show that different lengths of immersion in sea urchin steroid extract at a dose of 4 mg/L affect the formation of male individuals. A soaking time of 18 hours is quite effective in directing the sex of fish to males by 66%. Immersion time also affects the survival of tilapia larvae.
Water Quality Analysis Based On Diversity and Abundance of Macrozoobenthos In Way Semah River Waters of Pesawaran Irawan, Tazranisa Indy; Tugiyono, Tugiyono; Suratman Umar; Gregorius Nugroho Susanto
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.364

Abstract

River pollution is a major problem in the water quality crisis due to certain factors, including human activities around the river ecosystem which are strongly suspected to be the main cause of the problem. The purpose of this study was to determine the diversity and abundance of macrozoobenthos and the level of water pollution in the Way Semah Pesawaran River. The research was conducted using three indicators of water quality: biological indicators in the form of macrozoobenthos, physical indicators in the form of temperature and brightness, and chemical indicators in the form of pH, DO, and BOD. Sampling was carried out at 3 stations with two points, namely the right bank and left bank points at each station. The method of determining the location and data collection using the purposive sampling method. Based on the results of the analysis of abundance, diversity, uniformity and dominance of macrozoobenthos obtained the ecosystem of Way Semah River waters showed mild to moderate polluted conditions with a range of 1.21 - 1.87
Impact of Sewage Discharge on The Community Structure and Gonad Maturity of Fish in The Way Awi River Bandar Lampung Sa'diah, Annisa Nurul; Tugiyono, Tugiyono; Suratman Umar; Gregorius Nugroho Susanto
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 1 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i1.365

Abstract

Water pollution refers to the introduction of living organisms, substances, or other components by human activities that can degrade water quality or cause environmental harm. Gonad maturity level indicates the stage of readiness of prospective broodstock for the spawning process, assessed through visual and histological methods. This study aimed to assess water quality in the Way Awi River based on fish community structure and to evaluate the impact of sewage discharge on fish gonad maturity levels in Bandar Lampung's Way Awi River. Conducted between November and December 2023, this research sampled five points along the Way Awi River. Chemical analysis included pH, BOD, COD, and DO, while physical parameters such as temperature and TSS (Total Suspended Solids) were also analyzed. The findings indicate that the Way Awi River is moderately polluted, with no significant impact on the community structure, although there is a noticeable decrease in fish numbers from upstream to downstream. This study underscores the importance of understanding the risks associated with continuous disposal of waste into rivers.
Analisis Keanekaragaman dan Kelimpahan Plankton di Sungai Way Awi dan Hubungannya dengan Kualitas Air Pertiwi, Tina; Tugiyono, Tugiyono; Nugroho Susanto, Gregorius
Environmental Science Journal (esjo) : Jurnal Ilmu Lingkungan 2024: Volume 3 Nomor 1 Desember 2024
Publisher : Universitas PGRI Palembang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31851/esjo.v2i2.16628

Abstract

Plankton serves as a bioindicator that can be used as a marker for water quality related to water saprobity index. Way Awi River is a river flowing from the Susunan Baru area, passing through the Tandjungkarang region, and reaching the Garuntang area. Liquid waste from households is often directly discharged into the river, without passing through a containment system such as a septic tank, resulting in river pollution. To understand this relationship, this research was conducted to determine the biological condition of the Way Awi River based on plankton community structure, including abundance index, diversity index, evenness index, and dominance index, as well as its correlation with water quality using Pearson correlation test. The research was conducted in the Way Awi River with sampling taken at five different stations from October to December 2023. Water samples were analyzed using physical parameters including water temperature and turbidity, while chemical parameters observed were pH, DO, BOD, and COD. Based on the analysis of plankton community structure, it was found that the water of Way Awi River is in a moderately polluted condition.
Analysis of Microplastic at Sea Water and Sediment in the Pasaran Island Bay Using FT-IR Pamungkas, Galuh Rara; Widiastuti, Endang Linirin; Pratami, Gina Dania; Tugiyono, Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 11 No. 2 (2024)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v11i2.378

Abstract

Marine pollution from ocean garbage, particularly plastic waste, has significant ecological impacts globally. Plastic debris dominates marine pollution and graadually breaks down into microplastics. This study analyzes microplastic particle count, shapes, and polymer types in water and sediiment around Pasaran Island. Research was conducted across four stations (Station I, II, III, and IV).  Microplastics in seawater samples were extracted using solutions of 70% ethanol, 30% H2O2, and 30% NaCl. Sediments were treated with FeSO4 (0.05 M), NaCl, and 30% H2O2. Particle counts and shapes were identified with a digital microscope and polymer types were determined via Fourier Transform Infrared Spectroscopy (FT-IR). Microplastic forms observed included fibers, fragments, film, and pellets, with fibers being the most abundant and pellets the least. Detected polymers included polyamide (PA), polyethylene (PE), polypropylene (PP), polyvinyl chloride (PVC), polystyrene (PS), and polytetrafluoroethylene (PTFE), originating from both local plasstic use and seawater flow around Pasaran Island.
Keanekaragaman Makrozoobenthos di Sungai Way Umpu Kabupaten Way Kanan Provinsi Lampung Arsitalia, Metari; Tugiyono, Tugiyono; Master, Jani; Susanto, G. Nugroho
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 2 (2024): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i2.11524

Abstract

Way Umpu River, located in Way Kanan Regency, Lampung Province, is widely used by the local community and the waste products are ultimately disposed of into the river. This can cause a decrease in river water quality which can cause changes in the balance of the ecosystem, both biotic and abiotic. One of the organisms that can be used as a bioindicator is macrozoobenthos. This research aims to determine the diversity of macrozoobenthos as a bioindicator of water pollution quality. The method used was a survey method and sampling using Ekmen Grab at 6 sampling stations. Samples were taken 3 times in the left, middle and right bank areas of the river. Apart from that, water analysis was carried out in the form of physical parameters (temperature and TSS) and chemical parameters (pH, DO, COD, BOD). The data was then analyzed using the Diversity Index. The research results found 14 types of macrozoobenthos consisting of 5 phylum, namely Arthopoda, Mollusca, Porifera, Ciliophora, and Annelida. From the results of the analysis of the macrozoobenthos diversity index at station IV, it has a value of 0.56 ind/m2, which states that the location is heavily polluted. Physical and chemical factors show a significant correlation with macrozoobenthos diversity.
Faktor-faktor yang Berhubungan dengan Keberadaan Escherichia Coli (E. Coli) pada Makanan dan Minuman Sihaloho, San Maulina; Tugiyono, Tugiyono; Suharmanto, Suharmanto; Apriliana, Ety; Pramesona, Bayu Anggileo
Journal of Language and Health Vol 6 No 1 (2025): Journal of Language and Health
Publisher : CV. Global Health Science Group

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37287/jlh.v6i1.5736

Abstract

Bakteri Escherichia coli (E. coli) merupakan salah satu indikator untuk menilai penerapan sanitasi pangan. Infeksi bakteri E. coli meningkat setiap tahunnya. Tujuan literature review ini adalah untuk mengetahui faktor-faktor yang berhubungan dengan keberadaan E. coli pada makanan dan minuman. Literature review ini dilakukan dengan menelusuri artikel menggunakan search engine seperti Google Scholar dan PubMed. Artikel yang digunakan adalah artikel 10 tahun terakhir dari tahun 2015-2025. Kata kunci yang digunakan adalah keberadaan bakteri E. coli, makanan dan minuman dan penjamah makanan. Peneliti mendapatkan 300 artikel, dan 8 artikel yang memenuhi syarat untuk dilakukan analisis. Peneliti melakukan analisis hasil dan melakukan interpretasi hasil penelitian. Hasil penelitian mendapatkan bahwa adanya bakteri E.coli dalam makanan dapat disebabkan banyak faktor antara lain, air bersih yang mengandung E.coli, penjamah makanan yang kurang higienis, alat yang digunakan, bahan makanan, atau cara pengolahan makanan. Faktor yang berhubungan dengan keberadaan bakteri E. coli meliputi jarak sumber pencemar, kondisi dan konstruksi sumur yang tidak memenuhi syarat, serta higiene penjamah makanan. Ketidakberhasilan dalam menjaga kebersihan dapat menyebabkan kontaminasi, sehingga E. coli menjadi indikator adanya pencemaran, terutama dalam air minum dan makanan.
Terathogenic Testing of Black Cumin (Nigella sativa L.) Extract on The Number of Fetuses, Length of Front and Back Extremities, and The Number of Malformation Fetus in Mice (Mus musculus L.) Fardhira, Zikra; Nurcahyani, Nuning; Agustrina, Rochmah; Tugiyono, Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 9 No. 1 (2022)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v9i1.218

Abstract

Pemakaian obat tradisional telah berkembang dengan baik dan saat ini sudah banyak digunakan sebagai obat alternatif untuk mengatasi masalah kesehatan. Obat tradisional akan memberikan khasiat yang baik apabila dikonsumsi dengan cara dan dosis yang tepat untuk tujuan pengobatan. Berdasarkan penelitian sebelumnya pemberian jintan hitam (Nigella sativa L) terhadap mencit betina menyebabkan terjadinya aktivitas antifertilisasi, antinociceptif, kontrasepsi postcoitus, dan antioksitosin yang diduga dapat melancarkan menstruasi. Tingkat keamanan konsumsi jintan hitam pada masa kehamilan terhadap perkembangan fetus, serta sistem reproduksi mencit betina yang menimbulkan malfomasi congenital pada anaknya belum banyak diteliti. Tujuan dari penelitian ini untuk mengetahui pengaruh ekstrak jintan hitam (Nigella sativa L) terhadap jumlah fetus mencit,ukuran panjang ekstremitas depan dan belakang pada fetus mencit, dan jumlah fetus malformasi pada  mencit. Penelitian ini menggunakan 20 ekor  mencit betina yang sudah bunting dan dibagi menjadi 4 kelompok yaitu kelompok K (kontrol) diberi 0,3 mL aquabides, kelompok perlakuan P1 dengan dosis 2,1 mg/g BB dalam 0,3 mL aquabides, kelompok perlakuan P2 dengan dosis 8,4 mg/g BB dalam 0,3 mL aquabides, dan kelompok perlakuan P3 dengan dosis 33,6 mg/g BB dalam 0,3 mL aquabides. Perlakuan diberikan pada hari ke 6  sampai ke 17 kebuntingan. Hasil menunjukkan bahwa pemberian ekstrak jintan hitam tidak memberikan pengaruh terhadap jumlah fetus yang dikandung dan tidak menurunkan  pertumbuhan ekstrimitas depan dan belakang fetus mencit karena tidak menunjukkan hasil yang signifikan antara kelompok kontrol (K) dan kelompok perlakuan (P1, P2, dan P3). Pemberian ekstrak jintan hitam (Nigella sativa L.) tidak meningkatkan jumlah fetus malformasi akan tetapi menyebabkan adanya fetus yang mati pada saat kelahiran dan mengalami malformasi pada beberapa fetus lainnya.     The use of traditional medicine has developed well and is now widely used as an alternative medicine to treat health problems. Traditional medicine will provide good efficacy if consumed in the right way and dosed for medicinal purposes. Based on previous research, giving black cumin (Nigella sativa L) to female mice caused infertility, antinociceptive, postcoitus contraception, and anti-oxytocin activities which were thought to be able to launch menstruation. The safety level of consumption of black cumin during pregnancy on fetal development, as well as the reproductive system of female mice that cause congenital malformations in their offspring, has not been widely studied. The purpose of this study was to determine the effect of black cumin extract (Nigella sativa L) on the number of mice fetuses, the length of the front and back extremities in mice fetuses, and the number of malformed fetuses in mice. This study used 20 pregnant female mice and was divided into 4 groups, namely group K (control) was given 0.3 mL aquabidest, treatment group P1 with a dose of 2.1 mg/g BW in 0.3 mL aquabidest, treatment group P2 with a dose of 8.4 mg/g BW in 0.3 mL aquabidest, and the P3 treatment group with a dose of 33.6 mg/g BW in 0.3 mL aquabidest. The treatment was given on the 6th to 17th day of pregnancy. The results showed that the administration of black cumin extract had no effect on the number of fetuses conceived and did not reduce the growth of the front and rear extremities of the mice fetuses because it did not show significant results between the control group (K) and the treatment group (P1, P2, and P3). The administration of black cumin extract (Nigella sativa L.) did not increase the number of malformed fetuses but caused fetal death at birth and malformations in several other fetuses.      
Microplastic Analysis at Sea Water and Sediment in the Mahitam Island Lampung Bay using FT-IR Nita Sari; Widiastuti, Endang Linirin; Pratami, Gina Dania; Tugiyono, Tugiyono
Jurnal Ilmiah Biologi Eksperimen dan Keanekaragaman Hayati (J-BEKH) Vol. 10 No. 1 (2023)
Publisher : Department of Biology Faculty of Mathematics and Natural Sciences Universitas Lampung in collaboration with The Indonesian Association of Biology (PBI) Lampung Branch.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jbekh.v10i1.255

Abstract

The amount of garbage that accumulates in the waters causes environmental damage which is getting worse. The breakdown of plastic waste into smaller particles that have been degraded is called microplastic. This study aims to analyze the number of particles, shapes, and types of microplastic polymers found in the water and sediments of Mahitam Island. The research location was determined by purposive random sampling with three stations based on plastic sources, namely Station I, Station II, and Station III, which had different characteristics from laboratory-based testing for seawater and sediment. Microplastic analysis on seawater samples was carried out by adding a solution of 70% ethanol, 30% H2O2, and 30% NaCl, and on sediment samples was carried out by adding a solution of FeSO4 (0.05 M), NaCl, and 30% H2O2. The content of the number of particles and the shape of microplastics were analyzed using a microscope, while the analysis of microplastic polymers using FT-IR. The first thing to do is to extract microplastics The forms of microplastic found are Fiber, Film, Fragments, and Pellets. The highest number of microplastic particles was the type of film for water samples at Station 1 and Station 2 for sediment samples. The types of polymers found in water and sediments on Mahitam Island are polyvinyl chloride (PVC), polyethylene (PE), polypropylene (PP), and polystyrene (PS) which come from plastic waste. Microplastics are known to originate from the use of plastic originating from tourist activities and originating from the sea currents of Mahitam Island.
Co-Authors Agus Setyawan Ainin Niswati, Ainin Arlanda, Revilarita Arsitalia, Metari Ayuni, Ubaid Jan Bayu Anggileo Pramesona Daely, Sofia Vao Afni Dermiyati, Dermiyati Elly Lestari Rustiati Endah Setyaningrum, Endah Ety Apriliana Fardhira, Zikra Farisi, Salman Gina Dania Pratami, Gina Dania Hasan, Moh. Dwi Kurniawan Hutauruk, Elisa Marcelina Ida Betanursanti Indarto Indarto Irawan, Tazranisa Indy Jani Master, Jani Kurnietin, Dewinta Kusuma Handayani, Kusuma Lestari, Sepnina Like Nababan, Yolanda Nazma, Anya Khalisza Putri Nita Sari Nugroho Susanto, Gregorius Nuning Nurcahyani Nurul Khasanah, Nurul Pamungkas, Galuh Rara Pertiwi, Tina Pratiwi, Ratih - Prayogo, Muhaimi Mughni Priyambodo Priyambodo Qadar Hasani Ramadhina, Melati Rizki Rochmah Agustrina Sa'diah, Annisa Nurul Salman Alfarisi Sari, Mutiara Pradita Sihaloho, San Maulina Siti Romelah, Siti Suharmanto Supono Supono Suratman Umar Susanto, G. Nugroho Susanto, Gregorius Nugroho Uswatun Chasanah Widiastuti, Endang Linirin