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All Journal HAYATI Journal of Biosciences Biotika: Jurnal Ilmiah Biologi AL KAUNIYAH Jurnal Sain Peternakan Indonesia Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan Surya Octagon Interdisciplinary Journal of Science and Technology Floribunda ASEAN Journal of Community Engagement Current Research on Biosciences and Biotechnology Makara Journal of Science
Dwiranti, Astari
Departemen Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Indonesia, Depok, 16424, Indonesia, (021) 7270163.
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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Journal : HAYATI Journal of Biosciences

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The Development of Gametophyte Sterilization Method for Liverworts Acrolejeunea fertilis (Reinw., Blume and Nees) Schiffn. In vitro Culture Mouleidi Dwi Putri; Windri Handayani; Astari Dwiranti; Andi Salamah; Niarsi Merry Hemelda; Afiatry Putrika
HAYATI Journal of Biosciences Vol. 28 No. 1 (2021): January 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.1.39

Abstract

In vitro culture gametophytes of leafy liverworts often have problems in their sterilization process. These problems due to the high level of contamination and the fragile structure of the gametophyte leafy liverworts. The structures can be easily to damage after exposure to disinfectant. This study aimed to observe the concentration and the exposure time of “Bayclin” commercial bleach to suppress contamination with the viability of Acrolejeunea fertilisgametophytic explants. This research was conducted using control and 6 combination treatments with “Bayclin” concentration (1.00%, 1.25%, and 1.50%) and exposure time 60 and 120 seconds, then accompanied by the addition of Tetracycline 2.5 mg/ml. The qualitative parameters observed were the explant color, the type and location of contamination, and the growth of explants. The quantitative parameters were the percentage of contamination, the percentage of growth, and the number of new branches. The results showed that “Bayclin” 1.25% and 1.50% with 60 seconds exposure time has the lowest percentage of contamination which is 70% until the 7th days after planting. The most common type of internal contamination from the explant is bacteria and fungi. However, the growth of the new branch still occurs in some explants even though it has been contaminated and browned.
The Development of In Vitro Culture Sterilization Method of Gametophyte Explant Lopholejeunea sp. Anna Widyastuti; Afiatry Putrika; Astari Dwiranti; Andi Salamah; Niarsi Merry Hemelda; Windri Handayani
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.110

Abstract

In vitro cultures of leafy liverworts are still facing significant challenges due to high-level of explant contamination. The sterilization process can easily damage the structure of liverwort after exposure to the disinfectant. This study was to determine the concentration and time exposure of commercial bleach as a disinfectant to suppress contamination using the gametophyte culture of Lopholejeunea sp. The experiment consisted of control and six treatment combinations of commercial bleach with concentration 0.5, 0.75, and 1% (v/v), and exposure time (60 and 90 seconds). The type and location of contamination, the color of the explants after sterilization, and response after 30 days were observed. The results showed that the 0.75% bleach with 60 and 90 seconds exposure time had a lower contamination until the 7th day of culture. The most common type of contamination is bacteria and fungi that arise from the explant. Despite the contamination, it did not inhibit shoot formation. Further studies still needed to determine the type of fungicides and antibiotics with the most potent concentration and exposure time should be tested to obtain an axenic and viable culture of liverworts Lopholejeunea sp.
Co-Authors ., Dadun Abinawanto Abinawanto Abinawanto Adra Amalia Nur Ahlina Alwin, Wanarani Amira, Qanita Hana Andayani, Gitalisa Andi Salamah Anna Widyastuti ANOM BOWOLAKSONO Arief Boediono Atria, Mega Bani, Anna Puspitasari Daniel, Hisar Deti Purwanti Fadhilah Fadilah Febrina Rahmayanti Fitriana, Anis Funahashi, Hiroaki Harini, Melinda Ida Ruwaida, Ida Irawati, Yunia Iriani, Nur Annisa Isnaenisa Rachma Khayrani, Apriliana Cahya Kitty, Yelina Lischer, Kenny Luh Karunia Wahyuni, Luh Karunia Luzanil, Sonny Tirta Made Susiyanti Matsurah, Qaishum Menaldi, Adhityawarman Menaldi, Sri Linuwih SW Mouleidi Dwi Putri Nadhifa Tazkia Ramadhani Niarsi Merry Hemelda Niarsi Merry Hemelda Niarsi Merry Hemelda Nidaan Khafian Prakoso, Nurul Muhammad Priscilia, Florentina Putrika, Afiatry Rajagukguk, Selly Setiati Ratna Yuniati Rico Kurniawan Sabar Pambudi Sahid, Muhammad Hidayat SAHLAN, MUHAMAD Sarah Tsabituddinillah Sari, Elvita Shela Kartika Wijaya Siska Apriliani Sri Rahayu, Amy Yayuk Sundari, Ayu Mulia Tri Rahayu Utomo, Doddy Irawan Setyo Wicaksono, Gunawan Windri Handayani Windri Handayani Windri Handayani Wulandari, Tri Aprilliana Yeni Dwi Lestari