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Journal : Jurnal Biologi Tropis

Marketing Strategy of Mushroom Baglog in UMKM Agro Jamur Lombok, Gunungsari Sub-District, West Lombok Fadli, Fadli; Jupri, Ahmad; Kuncoro, Zain Bagus; Rozi, Tapaul; Hidayati, Lilik
Jurnal Biologi Tropis Vol. 25 No. 2 (2025): April-Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i2.9416

Abstract

White oyster mushroom is one of the most widely cultivated mushrooms in Indonesia because it has a high economic value. Umkm Agro Mushroom Lombok is one of the oyster mushroom and mushroom baglog cultivation businesses in West Lombok district. Apart from oyster mushrooms, in Umkm, mushroom baglogs also have a fairly high economic value with the right marketing and marketing strategy. One of the analytical tools that can be used in designing a marketing strategy is SWOT analysis. This analysis is able to identify strengths, weaknesses, opportunities and threats. The method in this study uses descriptive methods with data sources that come from direct sources or primary data and come from other sources or secondary data. The results of the analysis using SWOT show that quality and service are the strengths of Agro Mushroom Lombok, while the lack of promotion and baglogs that are vulnerable to weather are weaknesses, the potential that can be utilized is customer loyalty and a fairly wide market reach. The threats faced are the emergence of new competitors and limited raw materials. The strategies that can be applied are by prioritizing product quality so that the level of customer loyalty increases, besides that it is necessary to intensify promotion through social media to increase market reach.
Analysis of Production Costs for White Oyster Mushroom (Pleurotus ostreatus) Cultivation at UMKM Agro Jamur Lombok, Kekeri Village, Gunungsari District, West Lombok Regency Fadli, Fadli; Jupri, Ahmad; Maelia, Wina; Rozi, Tapaul; Hidayati, Lilik
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.9436

Abstract

Indonesia with its tropical climate, boasts abundant agricultural potential, including on Lombok Island. UMKM Agro Jamur Lombok is a promising white oyster mushroom cultivation business in this region, driven by high market demand. This research aims to analyze the production cost structure and business feasibility of white oyster mushroom cultivation at UMKM Agro Jamur Lombok. The study employed a descriptive quantitative method, collecting data through observation, interviews, questionnaires, and literature reviews. Data analysis included calculations of production costs (fixed and variable), revenue, income, and business feasibility using the R/C ratio, B/C ratio, and Break-Even Point (BEP). The research results indicate that UMKM Agro Jamur Lombok's total production cost in 2024 was IDR 197,100,000, with a revenue of IDR 336,000,000, yielding an income of IDR 138,900,000. The feasibility analysis showed an R/C ratio of 1.7047 and a B/C ratio of 0.7047, indicating that the business is feasible and profitable. Although there was a decrease in production due to damaged grow bags, the R/C ratio (1.6536) and B/C ratio (0.6536) after the decline still demonstrate business viability. However, there is a discrepancy between the BEP selling price of grow bags (IDR 8,213/unit) and the actual selling price in the field (IDR 4,000/unit), which requires further review regarding cost efficiency or market dynamics.
White Blood Cell Differentiation Based on Toll-Like Receptor 4 (TLR4) Gene Polymorphism in Local Indonesian Chickens Maskur, Maskur; Jan, Rahma; Rozi, Tapaul; Kasip, L.; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 24 No. 1 (2024): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1.6518

Abstract

Toll-like Receptors (TLRs) genes are a group of genes that transcribe TLRs proteins which act as receptors on the surface of phagocytic cells to recognize molecular patterns of pathogenic microbial components. This research aims to identify the variability of the TLR4 gene and its relationship with blood profiles in local Indonesian chickens. The samples used to identify TLR4 gene diversity in local Indonesian chickens were 135 samples. Research methods include DNA extraction, PCR amplification, RFLP analysis, and genotype determination. The genotyping results in the research were associated with a complete blood profile so that a genotype was obtained that could be used as a marker for resistance traits based on the blood profile. The results of this study showed that the TLR4 gene was polymorphic with three genotype types and an allele frequency of more than 0.01 in each KUB, Sensi, and Broiler CP 707 chicken. The frequency of the GG genotype is the highest compared to the AG and AA genotypes. White blood cell concentration values showed variations in the three genotypes of TLR4 gene and were still within normal standards, although statistically the differences were not significant.
Genetic Polymorphism of g.3768T>C loci of NRAMP1 gene in Kampung and KUB Chickens Maskur, Maskur; Muhsinin, Muhammad; Rozi, Tapaul; Jan, Rahma; Kasip, L.
Jurnal Biologi Tropis Vol. 24 No. 2 (2024): April - Juni
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i2.6682

Abstract

Natural Resistance-Associated Macrophage Protein 1 (NRAMP1) is a gene that regulates the body's resistance to illness. The genetic diversity of Kampung and KUB chicken population in Lombok island West Nusa Tenggara province was investigated based on g.3768T>C loci of NRAMP1 gene. Experimental animal consisted of 105 KUB and 67 Kampung chickens. The NRAMP1 gene polymorphisms in both population were identified using PCR-RFLP method. Genetic parameters of g.3768T>C loci were calculated using PopGene32. In this study, the locus g.3768T>C of the NRAMP1gene found three genotypes (TT, CT, CC) with the frequency of the C allele was higher than the T allele, respectively 0.709 and 0.210 in Kampung chicken, and 0.738 and 0.262 in KUB chicken. The mean value of observed heterozygosity (Ho) and expected heterozygosity (He) of g.3768T>C loci in both populations revealed low to medium genetic diversity, with the values 0.222 and 0.400, respectively. The mean value of observed heterozygosity is lower than expected, indicating that the population is heading towards a heterozygosity deficit and proving that the two populations are not in Hardy-Weinberg equilibrium. The mean PIC value of the NRAMP1 gene (g.3768T>C) in both chicken population was 0.319 and classified as fairly informative (0.50 > PIC > 0.25). Meanwhile, the value of the genetic differentiation index (Fst) for Kampung and KUB chicken population were 0.385 and 0.433, indicating that there is differentiation between two chicken population at the observed loci.
Body Weight and Morphometric Characteristics of KUB Chicken at 60 Days of Age Al Farizi, Muhammad Salman; Jan, Rahma; Maskur, Maskur; Rozi, Tapaul; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 24 No. 3 (2024): July - September
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i3.7319

Abstract

Kampung Unggul Balitnak Chicken (KUB) is a highly favored Indonesian native chicken breed among breeders because of its rapid development rate and ability to thrive in many situations. This study aimed to investigate KUB chickens' growth performance and morphometric parameters at 60 days of age, a crucial phase for determining their suitability as commercial broilers. Using an intensive rearing system, 100 KUB chickens were reared for 60 days in group cages. Body weight measurements were recorded weekly, and morphometric traits were measured after the study period. The study's findings indicated that the mean body weight of KUB chickens at 60 days of age was 710.53±16.76 grams, suggesting a favorable growth rate. In addition, the morphometric analysis revealed favorable physical characteristics, including an average chest length of 100.55±3.70 mm, chest width of 47.47±3.12 mm, wing length of 165.93±4.34 mm, back length of 164.9±4.26 mm, back height of 205.51±3.99 mm, shank length of 63.23±3.69 mm, and shank circumference of 35.76±1.48 mm. The results indicate that KUB chickens exhibit desirable body weight and morphometric traits at 60 days old, proving their suitability in commercial broiler farming. To summarize, this study offers significant information regarding the growth performance and physical characteristics of KUB chickens during a critical phase of their development. The findings highlight the breed's capacity to serve as a practical choice for commercial broiler production, enhancing the variety and long-term viability of the poultry sector in Indonesia.
Genetic Diversity of Bali Cattle Base on Two Microsatellite Loci - INRA032 and BM2113 Maskur, Maskur; Rozi, Tapaul; Rahmajan, Rahmajan; Kasip, Lalu; Muhsinin, M.
Jurnal Biologi Tropis Vol. 24 No. 1b (2024): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v24i1b.8076

Abstract

Microsatellites are short tandem repeat (STR) sequences that consist of simple repeats and exhibit a high number of alleles at each genomic locus. The aim of this study was to examine genetic variation in Bali cattle and the population dynamics using microsatellite markers. Two microsatellite loci, INRA032 and BM2113 were amplified using PCR Total DNA samples from the genome of 60 Bali cattle, then The PCR products were analyzed through agarose gel electrophoresis, followed by staining with Ethidium Bromide (EtBr). The number and size of alleles that appeared on the gel, while the diversity and population dynamics were analyzed using Popgene version 1.31 and Gene Calc. In this study, The effective number of alleles for the two microsatellite loci in the Bali cattle population analyzed was 6.53, with an average of 3.27. The average PIC of the two microsatellite loci was 0.70, while the observed heterozygosity (Ho) exceeded the expected heterozygosity (He), with values of 0.975 and 0.695, respectively. This suggests an excess of heterozygosity in the population and indicates that the populations are not in Hardy-Weinberg equilibrium. The inbreeding coefficient was high, with negative Fis values of -0.38 for INRA032 and -0.43 for BM2113.
Association of Inducible Nitric Oxide Synthase (iNOS) Gene Diversity with Immunity Characteristics in Kampung Chicken Muhsinin, Muhammad; Jan, Rahma; Maskur, Maskur; Rozi, Tapaul; Kasip, Lalu Muhammad; Al Farizi, Muhammad Salman
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8296

Abstract

The immune system of Kampung chickens is vital for maintaining health and productivity, especially in combating bacterial infections. Among the genetic components involved, the inducible nitric oxide synthase (iNOS) gene is crucial for producing nitric oxide (NO), a molecule with strong antimicrobial properties. This study investigated the association between iNOS (AluI│g.15056T>C) gene polymorphism and immune traits in Kampung chickens to enhance disease resistance. Blood samples from 100 Kampung chickens were genotyped using PCR-RFLP, and immune parameters such as leukocyte count, macrophage activity, and bacterial resistance were evaluated. Statistical analysis revealed significant differences among TT, TC, and CC genotypes. The CC genotype exhibited superior performance, including the highest macrophage activity (91.74±1.92%), activated macrophage capacity (2279.49), and bacterial death rate (60.81±3.54%). These findings suggest that the CC genotype enhances NO production, strengthening the immune response to bacterial pathogens like Staphylococcus aureus and Salmonella Pullorum. Additionally, the polymorphism contributes to improved genetic diversity and immune efficiency in Kampung chickens. The study highlights the potential of incorporating the iNOS CC genotype in selective breeding programs to produce chickens with enhanced resilience. Future research should focus on interacting environmental factors with iNOS expression to optimize its application in chicken production.
Identification of Diversity and Genetic Distance of Indonesian Local Chicken Strains Based on Myostatin Gene Muhsinin, Muhammad; Maskur, Maskur; Jan, Rahma; Rozi, Tapaul; Kasip, Lalu Muhammad; Al Farizi, Muhammad Salman
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8297

Abstract

Indonesian local chickens are essential genetic resources with significant potential for advancing the national poultry industry due to their high genetic diversity, reflecting adaptation to tropical environments and traditional farming systems. This study analyzed the genetic diversity of Kampung, Sentul, KUB, SenSi, and Broiler chickens based on the MSTN g.4842T>G locus. Blood samples from 150 chickens underwent PCR-RFLP analysis using the BsrI restriction enzyme targeting exon 2 of the MSTN gene. Parameters such as observed heterozygosity (Ho), expected heterozygosity (He), polymorphism information content (PIC), and Hardy-Weinberg equilibrium were assessed. Genetic distances were calculated using PHYLIP, and a dendrogram was constructed with UPGMA. The study identified two alleles (G and T) and three genotypes (GG, GT, TT), with the T allele being dominant, particularly in Broiler chickens (78.3%). Ho was lower than He in all populations, indicating a heterozygosity deficit. PIC values ranged from 0.282 (Broiler) to 0.367 (SenSi), classifying the MSTN locus as moderately polymorphic. Kampung and KUB chickens showed the closest genetic relationship, while Broiler chickens were the most distinct. These findings underscore the potential of local chickens in breeding programs for adaptability and productivity. Further research with additional markers is recommended to enhance sustainable poultry farming.
Identification of Single Nucleotide Polymorphism (SNP) in Exon 5 of the Prolactin Gene in Native Chickens Rozi, Tapaul; Maskur, Maskur; Jan, Rahma; Kasip, Lalu Muhammad; Muhsinin, Muhammad
Jurnal Biologi Tropis Vol. 25 No. 1 (2025): Januari - Maret
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i1.8342

Abstract

The prolactin gene (PRL) in chickens is one of the genes that regulates broodiness and egg production in laying hens. Single Nucleotide Polymorphism (SNP) is a form of single nucleotide variation in the DNA base sequence at specific locations in the genome that can influence differences in quantitative traits in livestock. This study aimed to identify SNPs in the prolactin gene of native chickens. The research methods included DNA extraction, PCR amplification, and DNA sequence analysis for genotype determination of individuals. The results revealed four mutation points at positions g.8052 bp T>C, g.7886 T>A, g.7823 A>G, and g.8069 T>C. All mutation points in exon 5 were substitution mutations. Statistical analysis showed that all loci were polymorphic and the population was in Hardy–Weinberg equilibrium.