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Effect of Ethanol Solvents in the Extraction Process of Bioactive Compounds from Brown Seaweed (Sargassum sp.) with the Ultrasound Assisted Extraction Method Slamet Pujianto; Boy Arief Fachri; Istiqomah Rahmawati; Erna Subroto
Journal of Biobased Chemicals Vol. 2 No. 2 (2022): Journal of Biobased Chemicals
Publisher : Program Studi Teknik Kimia Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jobc.v2i2.271

Abstract

Sargassum sp., part of brown algae, is traditionally used as animal feed. Nonetheless, Sargassum contains phenolic compounds that promise to be the raw material of natural antioxidants. The work is to extract phenolic compounds from Sargassum sp. in ethanol and to investigate the effect of the process on phenol yields. Several dried Sargassum sp. are milled in a certain size and extraction using ultrasound assisted extraction with ethanol solvent. Process variables are the extraction time (30-50 minutes) and the Ratio of Sargassum sp. to ethanol which is 5:100-15:100 (b/v), and power of 170-190 watts. Phenol compounds are quantified using the error acid method. The DPPH method is performed to check the antioxidant activity. To investigate the influence of the process, surface response methods based on central composite designs are applied in this work. 153.334 mgGAE/g in 30-minute extraction conditions, 170 watts of power, and a ratio between masses and solvents of 0.05. The antioxidant activity (IC50) of Sargassum sp. extract is 87.57 ppm.
Microwave Assisted Hydrolysis Ulva sp. Using HCl for the Production of Bioethanol Raw Materials Bekti Palupi; Nadia Ayumna Fa’iqoh; Alifia Rahma Putri Neysella; Boy Arief Fachri; Ditta Kharisma Yolanda Putri; Lukman Nulhakim; Maulida Septiyana
Journal of Biobased Chemicals Vol. 2 No. 2 (2022): Journal of Biobased Chemicals
Publisher : Program Studi Teknik Kimia Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jobc.v2i2.272

Abstract

The challenges faced by the Indonesian state are increasing greenhouse gases, climate change, and depleting fossil fuel reserves. This requires the exploration of alternative energy that is environmentally friendly and sustainable. Algae biomass, especially Ulva sp. is one of the resources that have the potential for bioethanol production as an alternative energy producer. The purpose of this study is to determine the potential of Ulva sp. as raw material for bioethanol and to determine the effect of particle size, solvent concentration, and power on the hydrolysis process. This study used Ulva sp. as raw material. hydrolyzed with HCl solvent using the microwave assisted hydrolysis method. Hydrolysis with HCl concentrations of 0.1 N, 1 N, 2 N, variations of microwave power 150 watts, 300 watts, 450 watts, and particle sizes of 60 mesh, 80 mesh, and 100 mesh. Measurement of reducing sugar levels was carried out using the dinitro salicylic acid (DNS) method. The results showed that the best conditions for hydrolysis were when the HCl concentration was 0.1 N, the microwave power was 450 watts, and the particle size was 80 mesh which resulted in a reducing sugar content of 20.751 mg/mL.
Extraction of Phenolic Active Compounds from Coffee Leaves (Coffea sp.) Using the Ultrasound Assisted Extraction Method and Total Phenol Analysis Alfinaini, Nur Aini Dwi Alfinaini; Boy Arief Fachri; Pratamai Shelli; Helda Wika Amini; Istiqomah Rahmawati
Journal of Biobased Chemicals Vol. 3 No. 1 (2023): Journal of Biobased Chemicals
Publisher : Program Studi Teknik Kimia Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jobc.v3i1.278

Abstract

Coffea sp. is one of the largest plantation commodities in Indonesia, especially coffee leaves containing phenolic compounds. This research was conducted to extract phenolic compounds using the Ultrasound-Assisted Extraction method with ethanol solvent which aims to determine the optimum operating conditions of extraction as well as the influence of variations in amplitude, time, and ratio of solvents produced. The experimental design was carried out using Design Expert 13 software with the response surface method box-Behnken design. The research variables used were amplitude variations (50%, 60%, and 70%), time (10, 20, and 30 minutes), and solvent ratios (0.1; 0.15; and 0.2 g/mL).Based on our study, these parameters affect the total phenolic content. The model equation for the total phenolic content of coffee leaves obtained is Y = 0.1349 – 0.0016 A – 0.0505 B + 0.0010 C + 0.0018 AB + 0.0043 AC – 0.0018 BC – 0.0004 A2 + 0.0178 B2 – 0.0014 C2 (R2 = 0.9758) with the optimum total phenolic content located in the 17th running of 0.209 mg GAE/g under conditions of 20 minutes, the ratio of material to solvent is 0.2 g/mL, and an amplitude of 50%.
Effect of Time Variation on Chlorophyll Concentration in Cocoa Leaf Extraction using the UAE (Ultrasonic Assisted Extraction) Method Rahmawati, Istiqomah; Shabrina, Nadhilah; Fachri, Boy Arief; Amini, Helda Wika; Palupi, Bekti; Rizkiana, Meta Fitri; Nurtsulutsiyah, Nurtsulutsiyah; Pradipta, Shima Nuril; Afwal, Diza Raudhatul; Reza, Muhammad
Jurnal Kartika Kimia Vol 5 No 1 (2022): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v5i1.97

Abstract

Until now, Cocoa (Theobroma cacao L.) plantation is a sector that has been continuously cultivated. Cocoa leaves contain chlorophyll, which is an important pigment in photosynthesis as sunlight absorber. The aim of this study is to determine the effect of extraction time to the concentration of chlorophyll in cocoa leaves. The extraction time was varied from 5 to 25 min with constant variables, namely power and ratio of raw materials. In this study, Ultrasonic Assisted Extraction (UAE) method was used due to its effectiveness. The concentration of obtained chlorophyll was determined by using UV-Vis spectrophotometry. The optimum extraction time was 15 min producing concentration of chlorophyll a of 9.39 mg/L, chlorophyll b of 4.78 mg/L, and total chlorophyll of 14.16 mg/L.
Optimization of Microwave Ultrasound Extraction of Biopolymer from using Response Surface Methodology Rahmawati, Istiqomah; Annisa, Yasmin; Farwah, Zilma Aliyah; Adrian, Febri; Anggraini, Ranita Anggi; Fachri, Boy Arief; Reza, Muhammad; Rizkiana, Meta Fitri; Palupi, Bekti; Amini, Helda Wika
Jurnal Kartika Kimia Vol 6 No 2 (2023): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, University of Jenderal Achmad Yani

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26874/jkk.v6i2.174

Abstract

This study aims to determine the effect of combining the two extraction methods Microwave Assisted Extraction (MAE) and Ultrasound-Assisted Extraction (UAE) on the yield and content of ulvan polysaccharides from Ulva lactuca with the best operation condition. Algae biomass U. lactuca is abundant, according to its content, U. lactuca contains polysaccharides which can be used as biodegradable plastic materials. The extraction was carried out using Microwave Ultrasound Extraction by varying the microwave power from 100 to 800 watts, the microwave time variations from 10 to 50 minutes, the ultrasonic wave temperature range from 40-80 oC, and the ultrasonic wave time variations from 20 to 50 minutes. Optimization was performed using the response surface methodology center composite design methodology for a total of 28 runs. Several tests were carried out to determine the character of the ulvan polysaccharide, including water content, FT-IR spectroscopy, High-Performance Liquid Chromatography, and its antioxidant activity. The effect of combining Microwave Assisted Extraction (MAE) and Ultrasound-Assisted Extraction (UAE) provides an optimal extraction condition with higher yields and good quality ulvan.
Preparation and Characterization of Cellulose Acetate from Pandanus tectorius via Microwave Irradiation Rizkiana, Meta Fitri; Amrullah, Abdul Malik Karim; Elisah, Nor; Amini, Helda Wika; Palupi, Bekti; Rahmawati, Istiqomah; Fachri, Boy Arief
Indonesian Journal of Chemical Research Vol 12 No 1 (2024): Edition for May 2024
Publisher : Jurusan Kimia, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/ijcr.2024.12-riz

Abstract

This research focuses on extracting cellulose from thorn pandan and evaluating its potential as a raw material for cellulose acetate. Thorn pandan contains polysaccharides, especially cellulose. In its fabrication process, cellulose acetate is produced from cellulose acetylation reaction. Cellulose is extracted using the Microwave-assisted Extraction method with variations in NaOH concentration (1, 2, and 3%), H2O2 concentration (10, 20, and 30%), and time variables (20, 40, and 60 minutes). Extraction optimization was performed with Response Surface Methodology (RSM) model BBD (Box-Behken Design). Several characterizations were carried out to determine the characteristics of cellulose acetate, namely Degree of Acetylation, FT-IR spectroscopy and X-Ray Diffraction (XRD). The highest cellulose yield obtained in the extraction process was 50.7% with a variable of 3% NaOH, 30% H2O2 and 60 minutes. XRD analysis shows the angle value 2θ = 22.53798° of thorn pandan cellulose structure is almost similar to commercial cellulose. FTIR functional group identification of cellulose acetate showed the presence of carbonyl (C=O) and (C-O Acetyl) group, respectively seen at wave numbers 1734 cm-1 and 1256 cm-1. The acetyl content of cellulose acetate from thorn pandan produced is 35.475%. Therefore, thorn pandan biomass has the potential as a raw material for making cellulose acetate.
Polyphenol Extraction from Musa corniculata Peel Using Microwave Assisted Extraction (MAE) Method with Ethanol Solvent Rahmawati, Istiqomah; Sa’roni, Achmad; Wardhani, Maharani Tri; Fachri, Boy Arief; Palupi, Bekti; Rizkiana, Meta Fitri; Amini, Helda Wika; Reza, Muhammad
BERKALA SAINSTEK Vol 12 No 2 (2024)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v12i2.41494

Abstract

Banana peel (Musa corniculata) is one of the superior plants in Indonesia which is rich in polyphenolic compounds as a source of antioxidants. The potential of polyphenolic compounds as antioxidants can be used as an alternative to reduce banana peel waste. This study aims to determine the total content of polyphenols and antioxidant activity of horn banana peel extract. Extraction used the Microwave Assisted Extraction (MAE) method with ethanol solvent and the variables were extraction time (4, 7, and 10 minutes), solvent concentration (50, 73 and 96 %), ratio of simplicia mass to solvent volume (1:12, 1 :8, 1:6 g/mL) with a microwave power of 150 Watt. Determination of polyphenol content using the Folin-Ciocalteau method and the ability of polyphenols as antioxidants were analyzed by DPPH test. This study used the Design Expert 13 Response Surface Methodology software, the Box Behnken type, to determine the extraction parameters for the total polyphenol content. The highest total polyphenol content was obtained at 354.02 mg GAE/g from the combination of parameters 50% solvent concentration, 10 minutes extraction time, and the ratio of horn banana peel powder to 1:8 g/mL solvent.
EKSTRAKSI PROTEIN DARI SPIRULINA SP DALAM PELARUT ETANOL TERBANTUKAN GELOMBANG ULTRASONIK Boy Arief Fachri; Bekti Palupi; Istiqomah Rahmawati; Meta Fitri Rizkiana; Helda Wika Amini
Jurnal Penelitian Sains dan Teknologi Indonesia Vol 1 No 1 (2022): Jurnal Hasil Penelitian UNIVERSITAS JEMBER
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat (LP2M) Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jpsti.v1i1.90

Abstract

Protein merupakan komponen nutrisi yang dibutuhkan oleh manusia untuk kesehatan tubuh. Salah satu sumber protein yang melimpah di Indonesia adalah alga Spirulina Sp. Ekstraksi protein alga, sulit dilakukan. Hal ini dikarenakan, protein tersimpan dalam sel-sel alga yang memiliki dinding yang sangat keras. Metode konvensional yang selama ini dilakukan membutuhkan waktu yang sangat lama, dan energi yang besar. Untuk mengatasi hal itu, sebuah metode alternatif yang diajukan dalam penelitian ini, adalah metode ekstraksi ultrasonik. Tujuan penelitian ini adalah untuk menentukan kondisi optimum ekstraksi protein dari Spirulina Sp dan mengetahui pengaruh parameter ekstraksi terhadap yield. Sprirulina Sp yang tekah dikeringkan dan dihaluskan diumpankan ke dalam ekstraktor yang berisi etanol. Gelombang ultrasonic, kemudian dialirkan ke dalam ekstraktor melalui probe. Setelah, eaktu ekstraksi tercapai, ekstrak dan residu dipisahkan dengan cara sentrifugasi dengan kecepatan 10000 rpm pada suhu kamar. Selanjutnya, yield protein ekstrak dihitung dengan analisa Lowry-Bradford. Pengaruh parameter proses seperti rasio bahan baku-pelarut (0:1-0.5) g/mL, ukuran partikel bahan baku (600-800) mesh dan waktu ekstraksi 15-45 menit) terhadap yield akan diamati dalam penelitian ini. Berdasarkan hasil penelitian, dapat disimpulkan bahwa yield tertinggi sebesar 80,87 % diperoleh pada kondisi rasio bahan baku-pelarut 0.2 g/mL, ukuran partikel bahan baku 700 mesh dan waktu ekstraksi 30 menit. Sementara itu, ukuran partikel merupakan parameter yang paling signifikan.
OPTIMASI EKSTRAKSI SENYAWA FENOLIK DAUN SIRIH MERAH (Piper Crocatum Ruitz & Pav) MENGGUNAKAN METODE MICROWAVE ASSISTED EXTRACTION (MAE) Amini, Helda Wika; Nugroho, Yeremia Prasetya; Rahman, Agus; Rizkiana, Meta Fitri; Rahmawati, Istiqomah; Palupi, Bekti; Fachri, Boy Arief
Dalton : Jurnal Pendidikan Kimia dan Ilmu Kimia Vol 7, No 3 (2024)
Publisher : Universitas Islam Kalimantan MAB Banjarmasin

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31602/dl.v7i3.16431

Abstract

Karena efek samping yang lebih sedikit daripada pengobatan sintetik, pengobatan menggunakan bahan alami menjadi pilihan populer di Indonesia. Oleh karena itu, diperlukan penelitian lebih lanjut mengenai bahan alami yang dapat digunakan sebagai obat, salah satunya adalah sirih merah. Penelitian ini bertujuan mengetahui kondisi optimal untuk mengekstraksi senyawa fenolik daun sirih merah dengan menggunakan metode Microwave Assisted Extraction (MAE). Penelitian ini menggunakan daun sirih merah ukuran 60 mesh yang telah dikeringkan. Dengan pelarut etanol 96%, daun sirih merah diekstraksi dengan variabel daya microwave 150 watt, 300 watt, dan 450 watt. Rasio bahan terhadap pelarut adalah 0,05 gram/mL, 0,1 gram/mL, dan 0,15 gram/mL. Ekstraksi berlangsung selama 5-15 menit. Analisis hasil penelitian dilakukan dengan analisis total fenol menggunakan metode Folin-Ciocalteu. Pada run ke-8, total fenol tertinggi adalah 0,0736 mg GAE/g, dengan rasio pelarut 0,05 g/mL, waktu ekstraksi 5 menit, dan daya microwave 300 watt.
Studi Awal Pembuatan Membran Chitosan-Silica Based dari Berbagai Limbah Setiawan, Felix Arie; Arimbawa, I Made; Putri, Navisa Ayudia; Fachri, Boy Arief
CHEESA: Chemical Engineering Research Articles Vol. 3 No. 1 (2020)
Publisher : Universitas PGRI Madiun

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (452.595 KB) | DOI: 10.25273/cheesa.v3i1.5701

Abstract

Jember memiliki potensi perikanan terutama udang sebagai sumber kitin yang merupakan dasar pembuatan kitosan. Kitosan berasal dari limbah kulit udang yang dicampur dengan limbah lain yaitu padatan silika dari Pembangkit Listrik Panas Bumi dan fly ash dari Pembangkit Listrik Tenaga Uap untuk menghasilkan membran yang dapat digunakan dalam filtrasi logam berat dalam air. Membran tanpa silika dibuat sebagai negative control dan dengan silika murni sebagai positive control. Membran kitosan dibuat dengan cara mencampurkan 1,0 gram kitosan dalam 100 mL larutan asam asetat 2% v/v dan 0,8 gram senyawa silika serta 0,5 gram polyethylene glycol. Membran yang telah dibuat diuji untuk menyaring larutan yang mengandung logam berat Cu dan Pb. Konsentratsi optimum dari Cu dan Pb dalam badan cairan dapat dikurangi sebesar 87% dan 80% menggunakan membran kitosan-silika.
Co-Authors Abdul Malik Karim Amrullah Adrian, Febri Afred, Merymistika Yufrani Afwal, Diza Raudhatul Ahmad Syuhri Aini, Dwi Intan Noer Ainia, Hilda Huril Akbar Anugrah Ikhsani Akmal, Gavinda Tsubutul Al Fajri, Maktum Muharja Alex Ttesa Elsyamba Alfinaini, Nur Aini Dwi Alfinaini Alifia Rahma Putri Neysella Anggraini, Ranita Anggi Annisa, Yasmin Ansori Ansori Ardila Dwi Tresna Ari Susanti Arief Budiman Aris Zainul Muttaqin Asshofy , Muhammad Agil Atiqa Rahmawati Atiqa Rahmawati Azzahrah, Anak Agung Nabilla Badril Azhar Badril Azhar Bekti Palupi Bimo Bayu Aji Buana, Nayyara Aida Danang Muharyanto Dianavita Fatimah Digdo Listyadi, Digdo Dihayat, Icha Shofia Dini Rahmawaty Ditta Kharisma Yolanda Ditta Kharisma Yolanda Putri Doddy Parningotan Elisah, Nor Elsyamba, Alex Ttesa Erna Subroto Farwah, Zilma Aliyah Fatimah, Dianavita Felix Arie Setiawan Firmansyah, Muhammad Sahrul Ghina Shofiah Adibah Khoirunnisa Gregah Pangayoman Hartanto P Habibatul Inayah Harti Arini Helda Wika Amini Helda Wika Amini Heni Pujiastuti Herdianto, Dimas Nur Hidayat, Debby Aurellia I Made Arimbawa Indrayani, Lilin Intan Hardiatama Istiqomah Rahmawati Istiqomah Rahmawati Kristianta, Franciscus Xaverius Lailatul Fatma Lestari, Afrila Tutut Dwijati Lukman Nulhakim Lumbantoruan, Sri Lestari M Edoward Ramadhan M Nurkoyim Kustanto M Yuda Pratama Maharani, Regita Gustiayu Pramisti Mahfud Mahfud Mahfud Mahfud Mahros Darsin Maktum Muharja Maulida Septiyana Meta Fitri Rizkiana Mizanurafi’ Ghifarhadi Prasiefa Mochamad Edoward Ramadhan Mohammad Nor Muhammad Reza Muhammad Reza Muharja, Maktum Mukhammad Fauzi Nabilah Lu’aili Fauziah Nadia Ayumna Fa’iqoh Naraismanti, Nirina Indi Nikita Meidi Nova Chintya Kurniawati Nova Chintya Kurniawati Nugraha, Briantara Agung Nugroho, Yeremia Prasetya Nur Karima Nur Karima Nurkoyim Kustanto Nurtsulutsiyah Nurtsulutsiyah Nurtsulutsiyah Nurtsulutsiyah Nurtsulutsiyah, Nurtsulutsiyah Permatasari, Ocita Galuh Pradipta, Shima Nuril Pratama, Ilyas Arya Pratamai Shelli Pravitasiwi, Nanda Tricya Julia Purimahua, Amanda Augre Maharani Puspita Sari Putri, Navisa Ayudia Putri, Salsabila Ananda R Rochmadi Rahman, Agus Ramadana, Ifan Ratnasonia, Samantha Githa Ratri Sekaringgalih Regita Gustiayu Pramisti Maharani Renald Rochman Mauludy Reswara Musyafa Rizalluddin, Muhammad Rizki Abdus Syatar Rizki Fitria Darmayanti Rizki Fitria Darmayanti Rizky Pratama, Reinaldi Rumaisha-Zuhriansyah, Rayya Safira Nur Oktavia Salsabila, Putri Ayu Santoso Mulyadi Sa’roni, Achmad Septianti, Kiki Shabrina, Nadhilah Sholeha, Irdatus Slamet Pujianto Suryagama, Muhammad Nabil Sutarsi, Suratsi Syatar, Rizki Abdus Tri Elok Setya Megasari Udroto, Firsta Retnaningtyas Uzinuzulla, Yunita Virda Wardani, Salza Belila Kusuma Wardhani, Maharani Tri Watiningsih, Lusi Karlina Wiwik Pratiwi Yakub Hendrikson Manurung Zuhriah Mumtazah