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All Journal E-Jurnal Medika Udayana Berkala Ilmu Kesehatan Kulit dan Kelamin Jurnal Biomedika dan Kesehatan JOURNAL LA MEDIHEALTICO MAHESA : Malahayati Health Student Journal Media Dermato-Venereologica Indonesiana Bali Dermatology Venereology and Aesthetic Journal
Winaya, Ketut Kwartantaya
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Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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Low Serum Vitamin E Levels Positively Correlate with the Severity of Acne Vulgaris Sutiawan, Indry Salonika; Winaya, Ketut Kwartantaya; Suryawati, Nyoman; Rusyati, Luh Made Mas; Karmila, I Gusti Ayu Agung Dwi; Puspawati, Ni Made Dwi
MAHESA : Malahayati Health Student Journal Vol 4, No 10 (2024): Volume 4 Nomor 10 (2024)
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/mahesa.v4i10.15882

Abstract

ABSTRACT Acne vulgaris (AV) is a common disorder of the pilosebaceous, especially prevalent in adolescents. Acne vulgaris ranks among the top ten most prevalent diseases globally. Although AV may resolve spontaneously, sequelae such as scar formation can persist, causing aesthetic and psychological disturbances. There is an antioxidant to counteract acne-causing oxidative stress, vitamin E, which is an important antioxidant in the human body. Therefore, vitamin E in the skin can reduce the formation of squalene peroxide and prevent the occurrence of AV. This is an analytical observational study using a cross-sectional design. The investigation was conducted at the Dermatology and Venereology Clinic of Prof. Dr. I.G.N.G. Ngoerah Denpasar Hospital and the laboratory of the Faculty of Medicine, Udayana University, from February 2024 until the sample size was fulfilled. The sample consisted of patients aged 12-45 years with acne vulgaris who visited the clinic and were then selected through consecutive sampling according to the inclusion and exclusion criteria. In the non-AV group, the mean serum vitamin E level was 19.3 ± 0.99 µg/ml, whereas in the AV group, it was 4.49 ± 19.1 µg/ml, indicating a significant variance. There was a positive correlation between low vitamin E levels and the severity of AV. The linearity graph shows the direction of the relationship between serum vitamin E levels and the severity of AV, which leads to the lower right, indicating that the higher the severity of AV, the lower the serum vitamin E levels. The mean levels differed significantly between the non-AV and AV groups. A favorable association was found between low vitamin E levels and the severity of AV. Keywords: Acne Vulgaris, Severity, Serum Vitamin E, Reactive Oxygen Species (ROS)
Pengaruh Pemberian Penyuluhan terhadap Stigma Penyakit Kusta pada Pasien dan Keluarga Kontak: Suatu Pre - Post Study Sari, Luh Putu Sustiana Kartika; Karna, N. L. P Ratih Vibriyanti; Rusyati, Luh Made Mas; Karmila, I. G. A. A Dwi; Puspawati, Ni Made Dwi; Winaya, Ketut Kwartantaya
Jurnal Biomedika dan Kesehatan Vol 8 No 3 (2025)
Publisher : Fakultas Kedokteran Universitas Trisakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18051/JBiomedKes.2025.v8.317-328

Abstract

Background Stigma toward leprosy patients remains a barrier to efforts to control this disease. Educational interventions are needed to increase public knowledge and reduce prejudice toward patients. This study aims to evaluate the effect of education on leprosy stigma among patients and their family contacts at Prof. Dr. I.G.N.G. Ngoerah General Hospital. Methods This study used a quasi-experimental design with a one-group pretest-posttest approach. The study was conducted at the Dermatology and Venereology Polyclinic of Prof. Dr. I.G.N.G. Ngoerah General Hospital in Denpasar, Bali, from February to July 2025. Data analysis was performed using SPSS version 26.0 at a significance level of α = 0.05. Results A total of 50 respondents were included, comprising 37 leprosy patients and 13 family members. A significant relationship was observed between stigma before and after health education (p = 0.031). Educational level (p = 0.001), clinical presentation (p = 0.002), and economic level (p = 0.029) were also significantly associated with stigma after health education. Respondents with higher education showed a significant decrease in stigma (OR = 11.245; 95% CI: 2.044–61.862; p = 0.005). Similarly, respondents with non-neurological disorders showed lower levels of stigma (OR = 12.415; 95% CI: 2.208–69.808; p = 0.004). Conclusions The findings of this study indicate that health education significantly reduced leprosy-related stigma, with educational level and clinical presentation identified as key determinants.
Comparison of Platelet, Leukocyte, and Erythrocyte Levels in Platelet-Rich Plasma with Centrifugation Speeds of 100 g/400 g and 300 g/750 g in Healthy Individuals Halim, Irene Andriani; Winaya, Ketut Kwartantaya; Puspawati, Ni Made Dwi; Darmaputra, I Gusti Nyoman; Suryawati, Nyoman; Indira, I Gusti Ayu Agung Elis
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol. 38 No. 1 (2026): APRIL
Publisher : Faculty of Medicine, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/bikkk.V38.1.2026.7-13

Abstract

Background: Background: Platelet-rich plasma (PRP) is an autologous blood product obtained through centrifugation and is widely applied in dermatology. Its cellular composition—particularly platelet, leukocyte, and erythrocyte levels—is influenced by various centrifugation parameters, with centrifugation speed being a critical factor. Purpose: This study aimed to compare the impact of two different centrifugation speeds, 100 g/400 g and 300 g/750 g, on the cellular composition of PRP. Methods: This cross-sectional study involved collecting blood samples from 34 healthy female subjects aged between 18 and 40 years. The samples were processed into PRP using two centrifugation speeds: 100g/400g (referred to as PRP1) and 300g/750g (referred to as PRP2). The counts of platelets, leukocytes, and erythrocytes were measured before and after centrifugation. Results: The mean platelet count in PRP2 (1,946.76 ± 436.98 × 10³/μL) was significantly higher than in PRP1 (1,445.00 ± 366.34 × 10³/μL; p = 0.000). The mean leukocyte count was significantly greater in PRP1 (58.69±22.82×10³/μL; p = 0.000) compared to PRP2 (32.68±16.20×10³/μL). Erythrocyte contamination was notably lower in PRP1 (0.16 ± 0.06 × 106/μL; p = 0.007) than in PRP2 (0.19 ± 0.06 × 106/μL). Multivariate regression analysis indicated that centrifugation speed was the most significant factor influencing variations in platelet, leukocyte, and erythrocyte counts (p < 0.05), while age and BMI had no significant impact. Conclusion: A centrifugation speed of 300 g/750 g was more effective in increasing platelet concentration but with erythrocyte levels, whereas the 100 g/400 g speed resulted in PRP with a higher leukocyte content.
Co-Authors Darmaputra, I Gusti Nyoman Edward Wijaya, Edward Els, Vanessa Halim, Irene Andriani Hernowo, Elliana Freya I Gusti Ayu Agung Dwi Karmila I Gusti Ayu Agung Praharsini I Gusti Nyoman Darmaputra Indira, I Gusti Ayu Agung Elis Karmila, I. G. A. A Dwi Karna, N. L. P Ratih Vibriyanti Kewatiting, Agnes Susanti Laksmi, Hermina Laksmi, I Gusti Ayu Agung Mini Limanda, Claudia Felicia Luh Made Mas Rusyati Ni Luh Putu Ratih Vibriyanti Karna Ni Made Dwi Puspawati Nyoman Suryawati Primasari, Putu Yunita Richard Richard Sadeli, Marrietta Sugiarti Sari, Luh Gede Melia Puspita Sari, Luh Putu Sustiana Kartika Stella, Aurelia Stephanie, Aurelia Sutiawan, Indry Salonika Triatmakusuma, Yogi Utami, Desak Nyoman Trisepti Wardhana, I Made