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All Journal e-Journal BUDIDAYA PERAIRAN JURNAL PESISIR DAN LAUT TROPIS JURNAL ILMIAH PLATAX
Kreckhoff, Reni L.
Staf Pengajar Pada Program Studi Budidaya Perairan FPIK UNSRAT
Earth & Planetary Sciences Environmental Science

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Uji penggunaan pelepah pisang dan spon pada pengemasan bibit rumput laut Kappahycus alvarezii dalam mencegah ice-ice Datangmanis, Adieng A. R.; Kreckhoff, Reni L.; Longdong, Sammy N. J.
e-Journal BUDIDAYA PERAIRAN Vol 8, No 2 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.2.2020.29980

Abstract

This search aimed to determine the shelf life of seaweed seedlings that are packaged using banana and sponge fronds in Styrofoam, determine the best seaweed packaging media in preventing damage or disease when seedlings are planted. The study was conducted for 5 weeks, from January to February 2020. Observation of seaweed seedlings was conducted at the Laboratory of Fish Health, Environment and Toxicology, Faculty of Fisheries and Marine Sciences and planting tests were carried out in Arakan Village, Tatapaan District. South Minahasa Regency.The research method was carried out in the form of: packing Kappaphycus alvarezii seaweed, observing seaweed seeds and planting test. The experiment was designed according to a factorial completely randomized 3 x 3 design so that there were two factors tested in this experiment, namely the packaging medium factor and the shelf life. The packaging medium factor (A), consists of the level ie, (a1) control / without medium, (a2) banana fronds, (a3) sponge; while the shelf life factor (B), consists of three levels namely, (b1) 24 hours, (b2) 36 hours and (b3) 48 hours with three treatments and three replications so that the total number is 27 units of experiment with the number of seeds needed as much as 108 kg. The treatments taken are A: control, B: Packaging seaweed seeds with the addition of banana midrib, C: Packaging seaweed seeds with the addition of a sponge. The data obtained were analyzed ANOVA statistics for factorial RAL using the JMP (SAS-institute) statistical program. The results of the analysis of the variance showed that differences in treatment (the combination between the storage medium and the shelf life) had a very significant effect on differences in the texture and color of seaweed ("P" <0.0001). However, the effect of the storage medium factor on the texture of seaweed did not change significantly at the time of the change in the shelf life factor level ("P" = 0.1664).
Penggunaan probiotik untuk meningkatkan respon imun nonspesifik ikan mas (Cyprinus carpio) Undi, Chintia S.; Manoppo, Henky; Kreckhoff, Reni L.; Tumbol, Reiny A.; Pangkey, Henneke
e-Journal BUDIDAYA PERAIRAN Vol 8, No 2 (2020)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.8.2.2020.29908

Abstract

The aim of this research was to count the total leukocytes and phagocytosis activity of goldfish seeds treated with probiotics. The test fish used were goldfish seeds measuring 5-8 cm. 500 fish will be taken from the Tateli Fish Cultivation and Development Center (BP3I). This study used a completely randomized design (CRD) with 5 treatments, where each treatment had 3 replications. The treatments used were probiotic bacteria from the intestines of goldfish with different concentrations. The treatments tested consisted of: A: without the addition of probiotics, B: addition of probiotics 1x106 cfu / mL, C: addition of probiotics 1x107 cfu / mL, D: addition of probiotics 1x108 cfu / mL, E: addition of probiotics 1x109 cfu / mL. Each concentration of bacteria that has been prepared is then sprayed on the commercial feed evenly using a sprayer and then dried to room temperature. After drying the feed was coated with 2% egg yolk and dried again at room temperature. Fish are stocked in an aquarium measuring 60x40x40cm3 with a density of 20 fish / aquarium. Each aquarium is equipped with an aerator and a water pump for recirculation. The fish were then given feed that had been added with probiotics at the same dose and frequency of administration as in the acclimatization process. The treatment is given for 6 weeks. At the end of the treatment feeding, fish samples were taken from each experimental unit to measure immune parameters, namely total leukocytes and phagocytic activity. Based on the results of research using the probiotic Lactobacillus sp. which originated from the intestines of goldfish and added to the feed had a significant effect on increasing total leukocytes and phagocytosis activity and the optimal concenration was  1x107 - 1x108 cfu / mL with a duration of 6 weeks of administration
Variasi komposisi daun Ketapang dan kulit pisang sebagai media tumbuh pakan alami jentik nyamuk Tununu, Riky R.; Solang, Jhonly; Pangkey, Henneke; Mingkid, Winda M.; Kreckhoff, Reni L.; Kusen, Diane J.; Wantasen, Adnan
e-Journal BUDIDAYA PERAIRAN Vol 9, No 2 (2021)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.9.2.2021.33752

Abstract

This study aimed to 1) determine the best combination between ketapang and banana peels for the propagation of life feed mosquito larvae; 2) determine the culture media that can produce mosquito larvae optimally. The containers used in this study were 24 buckets with a size of 5 litres. Some of the materials used in the study were dried ketapang leaves, goroho banana peels, kepok banana peels and water. This study used a factorial Completely Randomized Design (CRD) consisting of two factors and three replications. The first factor was the difference in the number of ketapang leaves. The second factor was the difference in banana peels types. The first factor included the difference in the number of ketapang leaves, consisting of: Treatment 0 = without ketapang leaves, Treatment 1 = 50 grams of ketapang leaves, Treatment 2 = 75 grams of ketapang leaves, Treatment 3 = 100 grams of ketapang leaves. The second factor included differences in banana peels, which consist of: Treatment 1 = one kepok banana peel, Treatment 2 = one goroho banana peel. The results were different types of banana peels affected the numbers of mosquito larvae, where the best was kepok banana peels; the difference in weight of ketapang leaves did not have a significant effect on the number of mosquito larvae. The container used in this study was a bucket with a volume of 5 L. The banana peels used were goroho banana peels and ripe yellow kapok banana peels. Ketapang leaves were preapared with a weight 0f 0, 50, 75, and 100 grams, respectively. Shoe banana peels and  goroho banana, one skin eachand ketapang leaves 0, 50, 75, and 100 grams, respectively, put together wit the jalah used to wrap between ketapang leavesand spatu banana peels and goroho banana, then put into a container that has been filled wit 3 L of water.
Uji efektivitas senyawa antibakteri penyebab ice-ice dari daun ketapang Terminalia catappa L. dengan metode ekstraksi berbeda Sinaga, Chintya R.; Kreckhoff, Reni L.; Salindeho, Indra R. N.; Ngangi, Edwin L. A.; Mudeng, Joppy D.; Rompas, Rizald M.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 1 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.1.2022.35527

Abstract

This study aimed to find out the right extraction method to produce ketapang leaf extract with best phytochemical content and best inhibition ability against ice-ice causing bacteria. The experiment was conducted at the Laboratory of Fish Health, Environmental and Toxicology, from April to June, 2021. The experiment was designed according to a Completely Randomized Design (CRD) with 5 treatments and 3 replications. The three main treatments were, treatment A: extraction of ketapang leaves Terminalia catappa L. with 95% ethanol (extract A), treatment B: extraction of ketapang leaves T. catappa L. with 40°C water (extract B), and treatment C, extraction of ketapang leaves T. catappa L. with distilled water (extract C).  In the inhibition ability test, each extract obtained from the three main treatments was compared with two controls namely treatment D: positive control (Kanamicyn) and treatment E: negative control (aquadest). The phytochemical content data was analyzed descriptively, while the bacterial inhibition zone diameter data was statistically analyzed with Anova and LSD. The phytochemical analysis result showed that, the extract A contains alkaloids, flavonoids, triterpenoids and tannins,  while extract B and extract C contain alkaloid, saponin and tannin. The inhibition ability test showed that extract A gave the strongest inhibition (14.87 mm) against the ice-ice causing bacteria, and was significantly different from that of extract B (5.23 mm) and C (2.47 mm).  The inhibition ability of extract B was significantly different from that of extract C. The inhibition ability of the three ketapang leaf extracts (A, B and C) against the ice-ice causing bacteria was much weaker and significantly different from that of Kanamicyn (positive control).
Pemanfaatan media dedak padi yang difermentasi dan tidak difermentasi untuk perbanyakan Alona sp. Sanggaria, Ayu W.; Solang, Jhonly; Pangkey, Henneke; Kreckhoff, Reni L.; Sambali, Haryani; Watung, Juliaan Ch.; Kambey, Alex D.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 1 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.1.2022.35498

Abstract

This study aimed to analyze the use of fermented rice bran media for the propagation of Alona sp. The method used is an experimental method, using one-way ANOVA, with three treatments, namely fermented rice bran media, unfermented rice bran media, and water. Each treatment was repeated 3 times, so that 9 experimental units were obtained. The observed variable was the density of Alona sp. The results showed that the density of Alona sp. that were cultured on fermented rice bran media was significantly different from that of unfermented rice bran and water.
Pemberian minyak pala (Myristica fragrans) Houttuyn 1774 sebagai bahan anestesi dengan konsentrasi berbeda pada benih lobster air tawar (Cherax quadricarinatus) Von Martens 1868 Mamuko, Nelsi; Mingkid, Winda M.; Watung, Juliaan Ch.; Kreckhoff, Reni L.; Longdong, Sammy N. J.; Manginsela, Fransine B.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 1 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.1.2022.35535

Abstract

This study aimed to examine the best concentration of nutmeg oil for anesthesia of freshwater crayfish (Cherax quadricarinatus) seeds and analyze the length of time to faint, the length of time to faint and the length of time to wake up for the seeds of freshwater crayfish (C. quadricarinatus) . This research was carried out at the Aquaculture Technology Laboratory, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University, Manado. The time of the research was carried out from April 18, 2021 - May 18, 2021. This study used a completely randomized design (CRD) consisting of 5 treatments and 3 replications, thus there were 15 experimental units. Treatment A with a dose of nutmeg oil (0.7 mL), treatment B (1 ml), treatment C (1.3 mL), treatment D (1.6 mL) and treatment E (1.9 mL). Parameters observed during behavioral research, length of time to faint, length of time to faint and length of time to wake up. The results of the study using the ANOVA statistical analysis test and the BNT follow-up test (Least Significant Difference) showed that different doses of nutmeg oil had a very significant effect on the length of time to faint, the length of time to faint and the length of time to wake up. The length of time to pass out the fastest, the longest length of time unconscious and conscious longest duration was obtained in treatment E (1.9 mL).
Identifikasi bakteri patogen Aeromonas sp. pada ikan Nila (Oreochromis niloticus) di Desa Matungkas, Kecamatan Dimembe, Kabupaten Minahasa Utara Sinubu, Wulan V.; Tumbol, Reiny A.; Undap, Suzanne L.; Manoppo, Henky; Kreckhoff, Reni L.
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.36633

Abstract

The purpose of this study was to identify the bacteria Aeromonas sp. in a tilapia (Oreochromis niloticus) culture pond in Matungkas Village, Dimembe District, North Minahasa Regency. Bacterial identification was carried out at the Laboratory of the Fish Quarantine Center for Quality and Safety Control of Fishery Products, Manado. The time of the study was carried out from February 2021 - June 2021. Ten samples of fish were selected from 3 existing ponds for this study with the criteria of fish that have clinical signs of disease infection such as pale body surface, red spots and ulcers. From 10 samples, 19 isolates were obtained to be identified. The bacterial method was carried out based on the results of gram tests and biochemical tests which included: Gram Tests, Motility Test, Oxidase Test, Oxidative Fermentative Test, Tolerance Test to incubation temperature, Tolerance Test to pH of the media, Tolerance Test to the concentration of NaCl in the media and rimler – shotts selective media test. Of the 19 isolates from 10 samples identified, there were 7 isolates that were positive for A. hydrophila, namely: N1.4.1, N2.1.1, N2.1.2, N2.2.1, N2.3.2, N3.3.1, and N3.3.2. Twelve other isolates were negative for A. hydrophila. The characters of A. hydrophila identified are creamy in colour, gram negative, oxidase positive, bacteria tolerance to temperature 4 °C positive, 37 °C positive, 50 °C negative, can move (motile), fermentative, pH tolerance test media pH 3 negative, pH 5 positive, pH 9 positive, pH 11 positive, tolerance test for NaCl concentration 0.5% positive, 3% positive, 5% negative, rimler – shotts positive. All sampling sites (ponds) in Matungkas Village were infected with bacteria.
Penggunaan ekstrak Ciplukan (Physalis angulata) untuk memacu pertumbuhan ikan Nila (Oreochromis niloticus) Maweikere, Fabiola C.; Tumbol, Reiny A.; Monijung, Revol D.; Manoppo, Henky; Kreckhoff, Reni L.; Darwisito, Suria
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.36635

Abstract

This study aimed to examine the effect of adding ciplukan (Physalis angulata) to feed on the growth of tilapia and to find a dose of ciplukan that was able to increase the growth of tilapia (Oreochromis niloticus). The test fish used were tilapia with an average size of 5-8 cm as many as 300 fish from the Center for Freshwater Aquaculture (BPBAT), Talelu. This research was conducted at the Aquaculture Technology Laboratory, Faculty of Fisheries and Marine Sciences, Sam Ratulangi University. This study used a completely randomized design (CRD) with 4 treatments, where each treatment had 3 replications so there were 15 experimental units, the treatment used was ciplukan flour with different concentrations consisting of: A: 0 g flour/kg feed, B : 10 g flour/ kg feed, C : 15 g flour/ kg feed, D : 20 g flour/ kg feed. The data collected consisted of fish weight to measure the fish growth. The results showed that the addition of ciplukan leaf extract in feed with different concentrations gave significantly different effects on the growth of tilapia with treatment B (10 g) showing the highest fish growth reaching 5.24 g, followed by treatment C (15 g) with the highest growth. reached 4.35 g. While the fish treated with D (20 g) only reached 3.16 g in weight where this value was not much different from the growth of fish in control A. Duncan's further test results showed that treatments B, C, D were not significantly different from each other, but these three treatments were very significantly different compared to fish that were not given ciplukan leaf extract. The study showed that ciplukan leaf extract added to feed gave significantly different effects on fish growth, with the best dose of 10 g extract/kg feed. Thus it can be concluded that ciplukan leaf extract can increase growth of Nile tilapia.
Pertumbuhan rumput laut Kappaphycus alvarezii yang menggunakan tali ris senar secara vertikultur Tindage, Tantri W.; Ngangi, Edwin L. A.; Kreckhoff, Reni L.; Mudeng, Joppy D.; Sambali, Hariyani
e-Journal BUDIDAYA PERAIRAN Vol 10, No 2 (2022)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.10.2.2022.36802

Abstract

The purpose of the study was to determine the growth of Kappaphycus alvarezii seaweed which was cultivated vertically using strings. The method is the use of the longline method vertically using strings. The experimental design was a completely randomized design with 3 treatments, namely different depths, and each treatment carried out 20 repetitions. Cultivation was carried out for 45 days, where seaweed growth measurements were carried out every 14 days. The highest absolute growth was in treatment A (2,675 grams) followed by treatment B (2,660 grams), treatment C (2480 grams). The highest daily growth rate was treatment B (6.79%) followed by treatment A (6.77%) and treatment C (6.55%). Furthermore, the results of the analysis showed that the absolute growth in treatments A and B was different from treatment C, as well as the daily growth rate which gave a very significant effect, where treatments A and B were very different from treatment C.
Uji fitokimia dan aktivitas antimikroba ekstrak kulit pisang kepok (Musa paradisiaca) terhadap bakteri Aeromonas hydrophila Aylifia S. Somba; Reni L. Kreckhoff; Diane J. Kusen; Henky Manoppo; Reiny A. Tumbol; Fitje Losung
e-Journal BUDIDAYA PERAIRAN Vol. 11 No. 1 (2023)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35800/bdp.11.1.2023.42192

Abstract

This research was conducted to qualitatively analyze the phytochemical content of kepok banana (Musa paradisiaca) peel extract and the effectivity of the extract as antibacterial against Aeromonas hydrophila. Fresh yellow color banana peels were washed, drained, then cut into pieces, crushed and dried at room temperature. Extraction was carried out by maceration method with 70% and 95% ethanol as solvent. A total of 90 g was put in an elemeyer glass then added with solvent until all the banana peels were submerged. The immersion time was 3x24 hours at room temperature while stirring occasionally so that the extraction occurs properly. Furthermore, the extract was filtered with Whatman paper no. 42 to separate the residue from the filtrate. The filtrate was then concentrated using a rotary evaporator. Phytochemical examined consisted of alkaloid, flavonoid, sternoid, triterpenoid, saponin, and tannin. Inhibition test against A. hydrophila was carried out by disc diffusion method using 5 different extract concentrations as treatments, namely A: 0%, B:10%, C:20%, D:30%, E:40%, F:50%. In the inhibition test, the paper disc was dipped in each treatment and then placed on agar media previously stocked with bacteria. The inhibitory zone was measured based on the clear area formed around the disc and observed at 24, 48, and 72 hours. Phytochemical test found that phenol and tannin were only detected in extracts with 95% ethanol while terpenoids and steroids were only found in  ethanol70%.  Flavonoid was detected in the extract using either ethanol70% and 95%. The results of the inhibitory test showed that the kepok banana peel extract could inhibit the growth of A. hydrophila bacteria after 48 hours of incubation. Inhibition zones formed at both 48 hours and 72 hours tended to increase in line with the increase in concentration even though the inhibitory power produced was categorized as "medium".
Co-Authors Adellin Dwiny Conchita Adnan Wantasen Alex D. Kambey Ali Djamhuri Aylifia S. Somba Billy Theodorus Wagey Cyska Lumenta Cyska Lumenta Dahlia Malau Datangmanis, Adieng A. R. Deiske A Sumilat Deiske Adeliene Sumilat, Deiske Adeliene Deiske Sumilat Dhebby Purba Diane J. Kusen Durand, Swenekhe S. Edwin D Ngangi Elvy Ginting Fitje Losung Fransine B. Manginsela Ginting, Elvi L. Grevo S Gerung Henky Manoppo Henky Manoppo Henneke Pangkey Indra R. N. Salindeho Indra R.N. Salindeho James J.H. Paulus Jasansong, Kalfianus Jhonly Solang Joppy Mudeng Juliaan Ch. Watung Kudato, Vania Kurniawan, Putra M. Kusen, Diane J. Kusen, Diane Joula Lauluw, Dafid Mamesah, Jeivana F.T Mamonto, Reva E. Mamuko, Nelsi Mantiri, Desy M. H Massora Dudung Maweikere, Fabiola C. Mokoginta, Leyda H. Mokolensang, Jeffrie F. Monijung, Revol D. Monijung, Revol J. Mudeng, Cindy C. Natalie D.C. Rumampuk Nego E. Bataragoa Ngangi, Edwin. L. A. Novie P.L. Pangemanan Ockstan J. Kalesaran Pangalila, Novelia M.A. Pangemanan, Novie P. L. Pangemanan, Novie P.L. Pankie N.L Pangemanan Pratasik, Gerald A. Prislawati Paputungan Putra, Stevano F.P Razak, Atiek P. Reiny A. Tumbol Reiny A. Tumbol Reiny A. Tumbol Rizald Rompas Rompas, Rizald M. Sambali, Hariyani Sambali, Haryani Sammy N. J. Longdong Sammy N. J. Longdong Sammy N.J. Longdong Sanggaria, Ayu W. Saragih, Debby Dyanessa Sartje Lantu Sartje Lantu Selvyane Bukasiang Shifa A. Schram Sinaga, Chintya R. Sinubu, Wulan V. Sipriana S. Tumembouw Suria Darwisito, Suria Suzanne L Undap Suzanne L. Undap Tindage, Tantri W. Tununu, Riky R. Umasugi, Aisin Undi, Chintia S. Usoh, Yosua Veibe Warouw Veibe Warouw Veibe Warouw Wantah, Monika M. Winda M. Mingkid Winda M. Mingkid Winda M. Mingkid, Winda M. Yuliana Macpal