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Kolo, Maria Magdalena
Program Studi Kimia, Fakultas Pertanian, Sains Dan Kesehatan, Universitas Timor, NTT, Indonesia
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Analisis Fitokimia Dan Uji Aktivitas Antioksidan Ekstrak Pelarut Kulit Batang Tumbuhan “At Anonse” (Annona reticulata L.) Obenu, Noviana Mery; Bria, Emilia Juliyanti; Kolo, Maria Magdalena; Mere, Jenrigo Klaumegio; Tse, Anastasia
Akta Kimia Indonesia Vol 9, No 1 (2024)
Publisher : LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j25493736.v9i1.17399

Abstract

Antioksidan adalah senyawa yang berfungsi untuk melawan radikal bebas dan melindungi sel dari efek toksik yang dihasilkan. Sumber antioksidan dapat berasal dari dalam dan luar tubuh. Antioksidan yang berasal dari luar tubuh dapat diperoleh dari tanaman yang mengandung senyawa metabolit sekunder seperti senyawa fenolik, flavonoid, alkaloid, tanin, steroid dan terpenoid. Berdasarkan hasil eksplorasi dan identifikasi tumbuhan Annona reticulata L. juga dimanfaatkan sebagai tumbuhan obat. Penelitian ini dilakukan dengan tujuan untuk mengetahui senyawa metabolit sekunder dalam ekstrak tumbuhan dan aktivitas antioksidannya. Ekstraksi dilakukan dengan metode maserasi menggunakan pelarut etil asetat dan metanol sedangkan uji aktivitas antioksidan di lakukan dengan metode DPPH (1,1-difenil-2-pikrilhidrazil). Hasil analisis fitokimia menunjukkan bahwa golongan senyawa yang terkandung dalam ekstrak etil asetat dan metanol adalah golongan triterpenoid, tanin, saponin, fenolik, flavonoid, dan alkaloid. Aktivitas antiosidan kedua ekstrak pelarut tersebut tergolong dalam kategori sedang dengan nilai IC50 ekstrak etil asetat sebesar 177,1325 μg/mL dan metanol sebesar 235,4198 μg/mL.
KAJIAN SIFAT KIMIA DAN FISKA TANAH PADA LAHAN BEKAS GALIAN MANGAN DI DESA OELAMI Tobing, Wilda Lumban; Taus, Jonisius; Bria, Deseriana; Tefa, Azor Yulianus; Kolo, Maria Magdalena
Agroprimatech Vol. 7 No. 2 (2023): Agroprimatech
Publisher : Prodi Agroteknologi Fakultas Agro Teknologi Universitas Prima Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.34012/agroprimatech.v7i1.4273

Abstract

Manganese is a micro nutrient that plants need in small quantities. Manganese plays an important role as an enzyme activator, including phosphate transfer enzymes and enzymes in the Krebs cycle. The aim of this research was to study the chemical and physical properties of the soil on ex-manganese excavated land in Oelami Village, TTU Regency. This research was carried out in June-September 2022. Analysis of the chemical and physical characteristics of the soil was carried out in the laboratory of the Faculty of Agriculture, University of Timor, and the Laboratory of the Faculty of Agriculture, Nusa Cendana University. This study used a descriptive experimental method which was carried out by survey and continued with laboratory analysis. Soil samples were taken at 5 points based on land use. Based on the results of the analysis of the chemical and physical properties of soil from ex-manganese excavated land, pH was 6.321 (slightly sour), CEC 33.46 me/100g (high), C-organic 0.22% (low), N-total 0.22% (low ), P-total 61.44 mg/100g (high), K-total 71.189 mg/100g (high), Mn-total 19,089 ppm (high), organic matter 0.81% (low), sandy loam soil texture ( sand 81.33%, dust 10.67%, and clay 8.00%), soil water content 8.611% (medium), soil density 1.25 g/cm3 (medium), soil specific gravity 1.47 g/ cm3 (low), and porosity 14.961% (high)
Quality of Complete Feed Wafer with Different Plant Protein Sources for Small Ruminants Feed Gerson Frans Bira; Azor Yulianus Tefa; Maria Magdalena Kolo; Cornelis Astridino Nitbani; Deni Yonathan Lulu; Agustinus Akoit
Livestock and Animal Research Vol 22, No 1 (2024): Livestock and Animal Research
Publisher : Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/lar.v22i1.68165

Abstract

Objective: This study aimed to assess the quality of complete feed wafers with different plant protein sources for a feed of small ruminants.Methods:The research was conducted in May-September 2022. The research method used a completely randomized design (CRD) with 4 treatments and 4 replications. The method used in this study was Completely Randomized Design (CRD) with 4 treatments, namely R0 = Wafer with an animal protein source (Fish meal) (Control), R1 = Wafers with protein source Sesbania grandiflora, R2 = Wafers with protein source Gliricidia sepium. R3=Wafer with protein source Leucaena leucocephala.The research variables consisted of physical quality (moisture content, wafer density, specific gravity, water absorption) and nutritional quality (dry matter, organic matter, crude protein, crude fat, crude fiber, and nitrogen free extracts). Data analysis according to the Analysis of Variance procedure using SPSS version 21.Results:The results showed that the use of plant protein sources had no significant effect (P>0.05) on water content and wafer density but had a significant effect (P<0.05) on specific gravity and water absorption capacity. The use of plant protein sources can increase the value of water content, wafer density, and specific gravity and reduce water absorption capacity. Meanwhile, the crude protein (CP) content of the wafer had no significant effect (P>0.05) and produced the same value as using fish meal. Other nutritional variables had a significant effect (P<0.05) such as DM, OM, EE and NFE which increased and CF decreased on the use of plant protein sources in making feed wafers compared to the use of fish meal.Conclusions: It was concluded that the use of legumes Sesbania grandiflora, Gliricidia sepium, and Leucaena leucocephala as a source of vegetable protein in wafers could replace fish meal. The three types of legumes had the same effect on the physical and nutritional quality of the resulting product.
Synthesis of Cellulose Acetate From Coil Waste (Borassus flabellifer L) Using Delignification Time Variations Matius Stefanus Batu; Maria Magdalena Kolo; Fransiska Rika
Stannum : Jurnal Sains dan Terapan Kimia Vol 6 No 2 (2024): October 2024
Publisher : Department of Chemistry - Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jstk.v6i2.4113

Abstract

Synthesis of cellulose acetate form palm coir (Borassus flabellifer L) waste has been synthesized by varying the time in the delignification process. This study aimed to determine the characteristics of cellulose acetate form palm coir waste (Borassus flabellifer L) and the optimum time for the delignification process. This research was conducted in several stages: sample preparation, cellulose isolation (delignification and bleaching process), and synthesis of cellulose acetate (activation, acetylation, and hydrolysis process). The cellulose acetate obtained was then charatecrized by testing for water content, acetyl content, and degree of substitution and functional grup analysis using a fourier transform infra-red (FTIR) spectrophotometer. In the delignification process, variations of the delignification time were usesd, namely 60, 90, 120, and 150 minutes. Form the results of the study, the characteristics of cellulose acetate in palm coir were optained, namely water content of 0.95%‒2.16%, acetyl content of 37.19%‒40.85%, degree of substitution of 2.2‒2.6 and identification of functional groups using FTIR showed that there was a typical absorption of cellulose acetate on the carbonyl group (C=O) and the ester group (C-O acetyl). The results of variations in the time of the delignification process showed that the optimum reaction time was 120 minutes with the yield of cellulose acetate produced at 254.4%, water content 2.16%, acetyl content 40.85%, degree of substitution (DS) 2.6. the cellulose acetate produced belongs to the type of cellulose diacetate, which can be further utilized in manufacturing threads, membranes, adhesives of films, and mask filters.
PENGGUNAAN LIMBAH KULIT PISANG LUAN (Musa paradisiaca) SEBAGAI ADSORBEN UNTUK MENGURANGI SALINITAS DAN ION KLORIDA PADA AIR SUMUR DI DESA LETNEO Matius Stefanus Batu; Pieter Jackson Lopez Dethan; Maria Magdalena Kolo
JURNAL INTEGRASI PROSES Vol 13, No 1 (2024)
Publisher : JURNAL INTEGRASI PROSES

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62870/jip.v13i1.24024

Abstract

Air sangat berperan penting dalam kehidupan makhluk hidup maka dibutuhkan sumber air yang memiliki kualitas dan kuantitas yang baik. Air sumur yang dimanfaatkan oleh masyarakat Desa Letneo tidak memenuhi syarat kesehatan sebab berwarna keruh serta memiliki rasa asin dan pahit ketika dikonsumsi. Salah satu cara untuk mengatasi permasalahan ini yaitu dengan metode adsorpsi menggunakan adsorben. Tujuan penelitian ini adalah mengetahui kandungan senyawa selulosa, hemiselulosa, dan lignin yang terdapat pada kulit pisang luan, menentukan karakteristik adsorben yang terbuat kulit pisang luan, dan kondisi optimum (massa adsorben dan waktu kontak) pada proses adsorpsi salinitas dan ion klorida dalam air sumur di Desa Letneo. Tahapan pada penelitian ini antara lain preparasi sampel kulit pisang luan, penentuan kandungan senyawa selulosa, hemiselulosa dan lignin yang terdapat pada kulit pisang luan dengan metode chesson-datta, aktivasi serbuk kulit pisang luan menggunakan aktivator H3PO4 2 M selama 24 jam, dan proses adsorpsi salinitas dan ion klorida dalam air sumur. Dari hasil penelitian diperoleh kulit pisang luan memiliki kandungan selulosa sebesar 13%, hemiselulosa 20%, lignin 23%, kadar air 2,6%, kadar abu 4,3%, dan bilangan iodin 3.173,25 mg/g. Hasil FTIR menunjukkan adanya gugus fungsi OH (hidroksi), C-H (alifatik), C=O (karbonil), C=C (alkena), C-H (bending) dan C-O. Hasil penentuan kondisi optimum dari proses adsorpsi diperoleh pada penggunaan massa adsorben 2,5 gram dengan kadar salinitas sebesar 0,10 ppt dan ion klorida 120 mg/L dan waktu kontak 30 menit dengan kadar salinitas sebesar 0,11 ppt dan ion klorida 76 mg/L
PEMBUATAN KITOSAN DARI LIMBAH CANGKANG KULIT UDANG WINDU (Penaeus monodon) ASAL KABUPATEN MALAKA Naisau, Mikhael Rifantus; Kolo, Maria Magdalena; Batu, Matius Stefanus
Journal of Chemical Science and Application Vol. 1 No. 2 (2023)
Publisher : Program Studi Kimia Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/jcsa.v1i2.4417

Abstract

Cangkang udang merupakan limbah yang dapat mencemari lingkungan. Bagian kepala dan cangkang udang dapat digunakan dalam proses pembuatan kitosan, mengandung kitin sebesar 20%-30%, protein 30%-40%, dan kalsium karbonat 30%-50%. Tujuan dari penelitian ini untuk mengetahui konsentrasi NaOH optimum pada proses deasetilasi kitin udang menjadi kitosan. Produksi kitin dilakukan melalui dua tahap yaitu demineralisasi dan deproteinasi. Proses deasetilasi dilakukan menggunakan larutan HCl 1 N, dengan perbandingan 1/15 (b/v). Reaksi deproteinasi dilakukan menggunakan larutan NaOH 3,5%, dengan perbandingan 1/10 (b/v). Reaksi deasetilasi kitin menjadi kitosan dilakukan dengan memvariasikan konsentrasi NaOH. Pada rentang 30%-70%, dengan perbandingan 1/10 (b/v), sambil dipanaskan selama 4 jam pada suhu 1200C dengan kecepatan 600 rpm. Derajat deasetilasi kitosan ditentukan menggunakan Spectroscopy FTIR (Fourier Transform Infrared). Hasil penelitian ini menunjukan bahwa konsentrasi NaOH optimum pada proses deasetilasi kitin limbah kulit cangkang udang windu menjadi kitosan yaitu 60% dengan perolehan rendemen sebesar 78,03%, kadar air 4%, kadar abu 0,04%, dan viskositas sebesar 3,5347±0,00 Cp.
Pendampingan Manajemen Desain Produk Pengolahan Limbah Pertanian Menjadi Pupuk Organik dan Eco-enzyme di Kelompok Tani Nunuh Naek Tefa, Azor Yulianus; Buan, Febrya Christin Handayani; Kolo, Maria Magdalena; Sipayung, Boanerges Putra; Tobing, Wilda Lumban; Mata, Meri Halsiana; Banunaek, Zofar Agluis; Manek, Seprianus Septian
Jurnal SOLMA Vol. 14 No. 1 (2025)
Publisher : Universitas Muhammadiyah Prof. DR. Hamka (UHAMKA Press)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/solma.v14i1.17652

Abstract

Background: Kegiatan pengabdian ini dilaksanakan bersama mitra kelompok Tani Nunuh Naek yang berlokasi di Desa Napan Kab.TTU, dengan jumlah anggota sebanyak dua puluh orang. Wilayah pertanian Kelompok Tani Nunuh Naek Desa Napan, memiliki sumber daya alam melimpah yang belum dikelola secara optimal, sehingga sering terbuang percuma dan menjadi limbah pertanian akibat keterbatasan wawasan dan ketrampilan petani. Kondisi agroekosistem lahan kering dengan solum dangkal, dan tingginya frekuensi serangan hama sering menyebabkan gagal panen. Sementara itu, ketergantungan petani pada penggunaan pupuk dan pestisida kimia meningkatkan biaya produksi, dan merusak lingkungan. Permasalahan ini menjadi dasar pelaksanaan kegiatan pengabdian yang bertujuan untuk meningkatkan pengetahuan dan ketrampilan petani mengolah limbah pertanian menjadi pupuk organik dan eco-enzyme. Metode: PkM ini menerapkan metode penyuluhan (edukasi) dan demonstrasi langsung (learning by doing) dengan pendekatan particypatory action research. Peningkatan pengetahuan dan ketrampilan mitra diukur berdasarkan instrumen kueioner. Hasil: PkM membuktikan adanya peningkatan wawasan dan ketrampilan petani mitra, serta perubahan perilaku dalam mengolah limbah pertanian menjadi pupuk organik dan eco-enzyme berkualitas, yang siap mewujudkan kemadirian pupuk dan biopestisida. Kesimpulan: Pengaplikasian pupuk organik dan eco-enzyme pada tanaman hortikultura telah berhasil meningkatkan produktivitas serta efektif menekan biaya produksi kelompok mitra. Selain itu, kondisi fisik, biologi dan kimia tanah juga mengalami perbaikan, menjadikan menjadi subur.
Pelatihan pembuatan Minyak Kelapa Murni (Virgin coconut oil) Menggunakan Metode Endapan/Pendiaman Di Kelompok Tani Efata Desa Sunsea Kecamatan Naibenu Kabupaten Timor Tengah Utara: Pelatihan pembuatan Minyak Kelapa Murni (Virgin coconut oil) Menggunakan Metode Endapan/Pendiaman Di Kelompok Tani Efata Desa Sunsea Kecamatan Naibenu Kabupaten Timor Tengah Utara Kolo, Maria Magdalena; Batu, Matius Stafenus
Bakti Cendana Vol 6 No 1 (2023): Bakti Cendana: Jurnal Pengabdian Masyarakat
Publisher : LPPM Universitas Timor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32938/bc.6.1.2023.26-36

Abstract

The Efata Farmers Group is a farmer group located in Sunsea Village, Naibenu District, North Central Timor Regency, whose daily activities are farming and raising livestock. Sunsea Village has coconut trees which produce coconuts in large quantities and are only used to make coconut oil by heating for cooking purposes. Even though coconut can produce pure oil or commonly called Virgin Coconut Oil (VCO). The purpose of this community service activity is to train members of the Sunsea Village Efata Farmer Group to produce quality Virgin Coconut Oil (VCO) oil products using a simple method. The activity implementation method uses focus group discussion (FGD). The activities carried out included training in VCO production using the settling/dwelling method, organoleptic testing of VCO quality and assessing the knowledge and understanding of partner groups. It was concluded that the partner group was very enthusiastic about participating in the activity as seen from attendance (100%). The stages of making VCO have been carried out and produced quality VCO from an organoleptic point of view, such as being clear in color and having a distinctive coconut smell. The partner group also experienced an increase in understanding and knowledge about VCO from 35% to 92.5%.
Isolation and Identification of Bacteria from Cattle Rumen Fluid and Their Application on In Vitro Digestibility of Rice Straw Bira, Gerson Frans; Sio, Stefanus; Batu, Matius S; Pardosi, Lukas; Kia, Kristoforus W; kolo, Maria Magdalena; Nubatonis, Alfred
Journal of Tropical Animal Science and Technology Vol. 7 No. 3 (2025): Journal of Tropical Animal Science and Technology
Publisher : Animal Husbandry Study Program, Faculty of Agriculture, Timor University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study aimed to isolate and identify bacteria from the rumen fluid of Bali cattle and to evaluate their effect as microbial inoculants on the in vitro digestibility of rice straw. The experiment was conducted using a Completely Randomized Design (CRD) consisting of four treatments and four replications. The treatments included rice straw silage with the addition of 0%, 5%, 10%, and 15% of local microorganisms (MOL) derived from cattle rumen fluid. The observed variables included the isolation and identification of bacteria from the rumen fluid, and the assessment of in vitro digestibility, including Dry Matter Digestibility (DMD), Organic Matter Digestibility (OMD), Volatile Fatty Acid (VFA) concentration, and ammonia (NH₃) production. The results showed that the addition of rumen fluid MOL significantly (P<0.05) improved the digestibility parameters. The highest DMD (52.44%) and OMD (47.11%) were obtained from the treatment with 15% MOL. VFA and NH₃ concentrations also increased, indicating enhanced microbial activity and fermentation efficiency. The study concludes that MOL derived from cattle rumen fluid, particularly those containing Bacillus sp., is effective in improving the nutritional quality and digestibility of rice straw
SYNTHESIS OF ACTIVATED CARBON FROM SORGUM STEM WASTE (Sorghum bicolor (L.) Moench) AS RHODAMIN B ADSORBENT WITH VARIATIONS OF NaOH ACTIVATOR CONCENTRATION Batu, Matius Stefanus; Kolo, Maria Magdalena; Teti, Roswita
Molluca Journal of Chemistry Education (MJoCE) Vol 15 No 2 (2025): MJoCE
Publisher : Program Studi Pendidikan Kimia, FKIP, Universitas Pattimura (Chemistry Education Study Program, Faculty of Teacher Training and Educational Sciences, Pattimura University)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/MJoCEvol15iss2pp59-67

Abstract

Rhodamine B is a synthetic dye commonly used, especially in the textile industry. Excessive entry of Rhodamine B into the environment can alter the pH of water, which can disturb microorganisms and animals living in aquatic environments. One way to address the pollution of Rhodamine B is by using activated carbon from sorghum stalk waste. Activated carbon is carbon that has been processed through activation to have pores with a very large surface area, thereby enhancing its adsorption capacity. The aim of this study is to determine the characteristics of activated carbon from sorghum stalk waste and to determine the optimum NaOH concentration in the synthesis of activated carbon from sorghum stalk waste as an adsorbent for Rhodamine B dye. The stages in this study include the preparation of sorghum stalk waste samples, carbonization, and activation of the carbon from sorghum stalk waste using NaOH activator with concentration variations of 2, 3, 4, 5, and 6% for 24 hours. The adsorption process was carried out using the activated carbon from sorghum stalk waste to adsorb Rhodamine B dye. The results of the study showed that the characteristics of the activated carbon from sorghum stalk waste had a moisture content of 4-7.3%, ash content of 2.3-7.6%, and an iodine adsorption capacity of 2,410.67-3,427.11 mg/g. The results of the characterization of activated carbon from sorghum stalk waste overall met the Indonesian National Standard (SNI). The optimum NaOH activator concentration for adsorbing Rhodamine B dye was obtained at a concentration of 6%, with an adsorption efficiency of 88.94%.
Co-Authors Adolvina Kono Agustina Rosalia Saka Agustinus Akoit Alfred Nubatonis Azor Y Tefa Azor Yulianus Tefa Batu, Matius S Batu, Matius Stafenus Bira, Gerson Frans Bria, Deseriana Bria, Emilia Juliyanti Cornelis Astridino Nitbani Deni Yonathan Lulu Edi, Eduardus Eduardus Yosef Neonbeni Efieno Maria Paulo Soares Eka Maria Sanit Elisa M. Thaal Emerensiana Naes Febrya Christin Handayani Buan Fransiska Rika Fredy Kurniawan Funan, Selviana Ermalinda Gelyaman, Gebhardus Djugian Gerson Frans Bira Gradiana Yosefa Nana Hilarius Y Sikone Jefry Presson jefry Presson Kia, Kristoforus W Korbafo, Elisabeth Lukas Pardosi Mading, Ridwan Mahardika Putra Purba Manek, Seprianus Septian Maria Fridolina Taek Maria Imaculata Mali Maria Rosanti Bani Mata, Meri Halsiana Matius Stefanus Batu Mere, Jenrigo Klaumegio Metriana Matilda Taus N. M. Obenu Naisau, Mikhael Rifantus Noviana Getrudis Abi Nugraha K. F. Dethan Oktovianus R Nahak Paulus Klau Tahuk Pieter Jackson Lopez Dethan Regina Seran Risna Erni Yati Adu Saka, Agustina Rosalia Sefrinus Maria Dolfi Kolo Sio Stefanus, Sio Sipayung, Boanerges Putra Taus, Jonisius Tea, Marselina Theresia Djue Teti, Roswita Tse, Anastasia Wilda Lumban Tobing Yakobus Pffeferius Edvend Saba Agu Yohana Ivana Kedang Yuni Sine Zofar Agluis Banunaek