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All Journal Dental Journal (Majalah Kedokteran Gigi) Biomolecular and Health Science Journal Conservative Dentistry Journal Indonesian Journal of Dental Medicine

Dian Agustin Wahjuningrum

Faculty Of Dental Medicine, Universitas Airlangga, Surabaya, Indonesia

Author-ID : 1563972

Biochemistry, Genetics & Molecular Biology Dentistry Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Perbedaan daya antiglukan NaOCl 2,5% dan ekstrak kulit manggis (Garcinia mangostana L.) 0,09% terhadap Enterococcus faecalis (Comparison of antiglucan activity between NaOCl 2.5% and mangosteen pericarp extract (Garcinia mangostana Linn) 0.09% against Enterococcus faecalis) Nabiela Rahardia; M Rulianto; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background. Failure of endodontic treatment is commonly caused by the persistent microorganisms remaining in the root canal such as Enterococcus faecalis. Enterococcus faecalis can form a biofilm in tough environmental conditions within the root canals and caused biofilm-mediated infections which needs more complicated treatment due to the increasing of antimicrobial resistance. The biofilm formation initial and most important step is bacteria adherence to the solid surface that is mediated by glucan. NaOCl 2.5% is a commonly used root canal medicaments but can cause injury of periapical tissue. Mangosteen pericarp extract contains flavonoid, tannin, and xanthone have mechanism for inhibiting adherence of bacterial biofilm. Difference of antibacterial activity between NaOCl 2.5% and mangosteen pericarp extract 0.09% can be determined by experimental laboratory to determine the adherence of bacteria in each treatment. Purpose. The aim of this study was to assess the difference of antiglucan activity between NaOCl 2.5% and mangosteen pericarp extract 0.09% on Enterococcus faecalis. Method. This study was designed as an experimental laboratory study with post test only control group design using Enterococcus faecalis ATCC 29212. Mangosteen pericarp was extracted using maceration method. Adherence analysis was observed after 24 hours by examining the viable cells in suspension. These viable cells are measured by UV-Vis spectrophotometer to compare the suspensions’ turbidity. Using the Independent T-Test, significantly less bacteria were found adhering to the mangosteen pericarp extract. Results. Absorbancy difference level by mangosteen pericarp extract 0.09% is significantly greater than the NaOCl 2.5% (p<0.05). Conclusion. Antiglucan activity that generated by mangosteen pericarp extract 0.09% is greater than NaOCl 2.5%

Perbedaan daya pembersih kavitas saponin ekstrak kulit manggis (Garcinia mangostana Linn) 0,78% dan asam sitrat 6% (The difference of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity) Ivon Dewi Setianingrum; Ketut Suardita; Ari Subiyanto; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background: Cleanliness of cavity is considered important for a restoration. Smear layer formed after cavity preparation should be removed in order not to disrupt the bond adhesion between restorative materials and dental cavities. Saponins contained in mangosteen pericarp (Garcinia mangostana L.) have surfactant properties that can eliminate the smear layer assessed. 6% citric acid is a chelating agent which can eliminate the inorganic particles of the smear layer. Until now, the research on the differences of 0,78% saponin from mangosteen pericarp extract and 6% citric acid for cleanliness of cavity has never been done. Purpose: To see the differences between 0,78% saponin from mangosteen pericarp extract and 6% citric acid as cavity cleanser. Method: Eighteen human teeth with complete crown, no caries, and no fractures were randomized in 3 groups (n≥6), in this experiment use (n=6). The cavity was prepared using wheels bur for hand use instrument. After instrumentation, each cavity on the first group used 0,78% saponin from mangosteen pericarp extract as cavity cleanser, the second group used 6% citric acid as cavity cleanser, and the control group used aquadest. Then, the teeth were split to be observed on Scanning Electron Microscope (SEM). Result: For Mann- Whitney test there were significant differences just between 078% saponin from mangosteen pericarp extract with 6% citric acid, and 6% citric acid with aquadest, but not for 0,78% saponin from mangosteen pericarp extract with aquadest. Median value of 6% citric acid showed 2,000 which is the smallest value compared to the value of the other groups. Conclusion: The cleanliness of cavity with 6% citric acid is better than that with 0,78% saponin from mangosteen pericarp extract.

Effectiveness of flavonoid from mangosteen pericarp (Garcinia mangostana L.) as Enterococcus faecalis antibiofilm Dalhar Hakiki; Latief Mooduto; Ketut Suardita; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 7 No. 1 (2017): January - June
Publisher : Universitas Airlangga

Background:Enterococcus faecalis (E. faecalis) is a microorganism that is commonly found in endodontic failure treatment, this due to several characteristics of E.faecalis which has the capabillity to living in environments with high salt levels, high temperature, and pH broad spectrum. Bacteria in biofilms form is one of the adaptive process that allows bacteria to survive in an environment with low nutrients in the root canals. Bacteria in biofilms form have different characteristics from planktonic form, resistance to phagocytic cells and drugs, which can effect to persistent infection. Mangosteen (Garcinia mangostana) has many benefits, especially on the pericarp of the fruit contains alkaloids, tannins, phenolics, flavonoids, and triterpenoids. Flavonoids are the largest group of phenolic compounds that have a nature effectively inhibit the growth of viruses, bacteria, and fungi. Purpose:Purpose of this study wasto find out the role of the antibiofilm of the flavonoid in garcinia mangostana pericarp against E. faecalis bacterial biofilm. Methods:Laboratory experimental in-vitro with post test only group design. The method used is microtitter plate biofilm assay and continued with the readings use Elisa reader at a wavelength of 595 nm. Results:Flavonoids mangosteen pericarp effective as antibiofilm E.faecalis bacteria at a concentration of 12.5%. Conclusion:The study showed that flavonoids from mangosteen pericarp has antibiofilm activity against E. faecalis bacterial biofilm.

EKSPRESI MATRIKS METALLOPROTEINASE-8 DAN INTERLEUKIN-8 PADA KERUSAKAN JARINGAN PERIAPIKAL AKIBAT INDUKSI BAKTERI ENTEROCOCCUS FAECALIS Tamara Yuanita; Tantri Wismayaning Radito; Dian Agustin wahjuningrum; R. Roulianto
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-8 to enhance the inflamation reaction and MMP-8 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of MMP-8 and IL-8 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-8 and IL-8 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of MMP-8 and IL-8 are increasing during the periapical tissue damage that induced by E.faecalis.

Endodontic Retreatment In Case Of Failure: Case Report Kevin Prayogo; Dian Agustin Wahjuningrum; Ari Subiyanto
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: In medical practice, clinicians come across a lot of obstacle during a treatment , that can lead to a failure, like other dental treatments, endodontics too can fail. Inappropriate mechanical debridement, persistence of bacteria in the canals and apex, poor obturation quality, over and under extension of the root canal filling, and coronal leakage are some of the commonly attributable causes of failure. Despite the high success rate of endodontic treatment, failures do occur in a large number of cases and most of the times can be attributed to the already stated causes. Purpose: Endodontic retreatment of a failure is required by the increased desire to preserve the tooth on the dental arch, preventing the need for dental extraction that may have adverse consequences in terms of functional and psychological effect on patients. Case: This article presents a case report about dental retreatment with 2 common failure, that was under filling obturation and non-fit post. Case management: The failure was corrected with endodontic retreatment, and finally restored with fiber post and porcelain fused to metal crown. Conclusion: Endodontic retreatment was done successfully on underfilling obturation and non-fit post. The patient was satisfied with the result.

Biocompatibility of 0,78% tannin of garciniamangostanalinnpericarp extract and 0,2% chlorhexidinegluconateagainst BHK-21 fibroblast cells culture Monika Werdiningsih; Ira Widjiastuti; Febriastuti Cahyani; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background.Chlorhexidinegluconate is one of endodontic irrigants potential due to its antibacterial activity. Although it is an effective antibacterial agent, chlorhexidinegluconate cannotdissolve organic substances and necrotic tissue present in the root canal. In addition, same as other chemicals, chlorhexidinegluconate also cause a cytotoxic effect. Tannin extracts of mangosteenpericarp (Garciniamangostana Linn.) demonstrated various biological activities including antibacterial, antioxidant, anticancer and anti-inflammatory. Tannin extracts of mangosteenpericarp can be considered as an alternative endodontic irrigation for dental application.Purpose.The aim of this study was to compare the biocompatibility between 0,78% tannin extracts of mangosteen pericarp and 0,2% chlorhexidinegluconate to BHK-21 fibroblass cell. Method.Tannins obtained from extracts of mangosteenpericarp. Preliminary test was conducted in the biocompatibility of tannin extracts of mangosteenpericarp in various concentrations of the BHK-21 fibroblass. Tannins extracts of mangosteenpericarp was at concentrations of 0,78% less toxic than the other concentrations. Then biocompatibility of 0,78% tannin extracts of mangosteenpericarp compare with 0.2% chlorhexidinegluconate using cytotoxicity test on BHK-21 fibroblass cells with MTT method and observed by ELISA reader. Results.0,78% tannins extracts of mangosteenpericarp showed good biocompatibility with fibroblass BHK-21 than 0,2% chlorhexidinegluconate. There was a significant differences between the results of treatment with 0,78% tannin extracts of mangosteenpericarp and 0.2% chlorhexidinegluconate. Conclusion. 0,78% tannin extracts of mangosteenpericarp has better biocompatibility than 0,2% chlorhexidinegluconate as an endodontic irrigants.

The differences between mangosteen peel extract irrigant and NaOCl 2.5% on root canal cleanliness Cornelia Melinda Adi Santoso; Karlina Samadi; Eric Priyo Prasetyo; Dian Agustin Wahjuningrum
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background: Preparation, one of the stages in root canal treatment, always producesdebrisas aresultof instrumentation onroot canalwalls. Debrisconsistsoforganicand inorganicmaterials. Irrigation need to be performed in every preparation so that debrisandmicroorganisms can be removed from root canal wallsthrough flushingmechanism. NaOCl 2.5% is the most popular irrigant used in root canal treatment. However, NaOCl2.5% onlyworks onorganic tissue. Mangosteenpeel extractcontainsvariousactive compounds, such as saponin. Saponin acts assurfactantso as tolower thesurface tension and remove debris from the root canalwalls. Purpose: to compare the cleanliness of root canal walls following irrigation with NaOCl 2.5% and mangosteen peel extract. Method: Eighteen mandible premolar extracted for orthodontics necessity were used in this study. The teeth were divided into three groups, in which each group consists of six teeth. All of them were instrumented with ProTaper for Hand Use and irrigated. Group 1 used aquadest as irrigant, group 2 used NaOCl 2,5%, and group 3 used mangosteen peel extract 400 ug/ml. The roots were split longitudinally into halves and in 1/3 of apex. The surface of the canal walls were examined using scanning electron microscope. Photomicrographs were scored by three independent observers and statistically tested. Result: There were significant differences between three groups (p<0.05). Group 3, irrigated with mangosteen peel extract 400 ug/ml, had the smallest median score that was 1. Conclusion: Mangosteen peel extract is moreeffective thanNaOCl 2.5% incleaningroot canalwall from debris.

The Difference of Irrigant Solution between Red Pine Extract (Pinus densiflora) and Green Pine Extract (Pinus merkusii) Against Cleanliness of Root Canal Walls Dian Agustin Wahjuningrum; Setyabudi Setyabudi; Sukaton Sukaton; Yuline Krishartini
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.62-65

Background: Smear layer is an accumulation of organic material, debris, and inorganic material in root canal wall. Irrigant solution must be able to eliminate organic and inorganic materials. Herbal extract such as pine contain saponins that can dissolve organic and inorganic materials. Korea is a country that has used pine in this case red pine as an antibacterial medicament. In Indonesia, there are also green pine species. Purpose: To explain the differences in cleanliness of the root canals after irrigation using extracts of red pine (Pinus densiflora) and green pine (Pinus merkusii). Methods: This study used twenty-seven mandibular premolar teeth which were divided into 3 groups each group consisted of 9 premolar teeth. All samples were prepared using ProTaper for Hand Use and irrigated. Group I was irrigated using 2.5% NaOCl, group II was irrigated with red pine extract, and group III was irrigated using green pine extract. Tooth that has been prepared, were cut vertically into 2 and cut horizontally in 1/3 apical section. The root canal surface was observed using a scanning electron microscope. Photomicrograph was observed by three observers using a score and performed an analysis test. Results: There were no difference between red pine (Pinus densiflora) and green pine (Pinus merkusii) in root canal cleanliness with a significance value of 0.169 (p> 0.05) Conclusion: Red pine extract had the same smear layer cleaning power as green pine extract.

One visit endodontic on asymtomatic mummificated mandibular molar Sholeh Ardjanggi; Dian Agustin Wahjuningrum; Tamara Yuanita; Eric Priyo Prasetyo
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.80-82

Background. The main goal of root canal treatment is the prevention or treatment of apical periodontitis, which leads to the preservation of natural teeth. Traditionally, root canal treatment is carried out in a number of visits, using extra disinfecting agents in addition to irrigant used during cleaning and shaping procedures that primarily aim to reduce or eliminate microorganisms and their byproducts. from the root canal system before it is obtained. Recent advances in Endodontic technology, attracting dental experts and endodontists to perform root canal treatment in one visit. Numerous studies evaluate the effectiveness of single-versus multiple-appointment root canal treatment have been published, which reported no significant differences in effectiveness (healing rates) between these two treatment regimens.Purpose : preservation of the integrity of the molar teeth that have symptomatic complaints by taking proper care with a short visit time. Caset: A 21-year-old woman presents with concern in her lower left tooth, the patient tells that the tooth had been treated before at the dentist when she was a child. On clinical examination there is a composite restoration in occlusal tooth # 36, non vital teeth. On periapical radiology there are radiopaque on pulp chamber and a diffuse radiolucent area in the apical area of tooth # 36, then the diagnosis then determined Previously initiated therapy with asymptomatic apical periodontitis. Case Management : Tooth # 36 treated with one visit root canal treatment, Treatment is continued with fiber post installation and crown, patients no longer complain of pain after treatment. Teeth function normally again. Conclusion: One visit root canal treatment can successfully eliminate patient complaints and restore tooth function.

The Difference of Antibacterial Power Between Cocoa Peel (Theobroma cacao) Extract 6% Compared to NaOCL 5% Againts Lactobacillus acidophilus Tamara Yuanita; Dian Agustin Wahjuningrum; Mega Selvia
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.67-71

Background: Lactobacillus acidophilus is a gram-positive rod-shaped bacteria which mostly causes chronic caries lesions. In the process of cavity preparation it is reported that it is not possible to remove all microorganisms and even bacteria can survive even after removal of the necrotic tissue. Efforts to stop the growth of these bacteria are by chemical cavity cleanser using a 5% NaOCl, but this material have disadvantages including irritating periradicular tissue, unpleasant odor, and toxicity. Due to its disadvantages, it is hoped that natural ingredients can be used as alternatives. Cocoa peel extract has active compounds of tannins, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial ability with a concentration of 6% according to the non-toxic minimum kill concentration of Lactobacillus acidophilus. Purpose: To analyze the difference in antibacterial power between the extract of cocoa bark with a concentration of 6% compared to 5% NaOCl against Lactobacillus acidophilus. Methods: This study was a laboratory experimental in vitro with a post test-only control group design. Using diffusion method against Lactobacillus acidophilus grown in tubes containing BHIB, then cultured in a petridish which contains a nutrient agar and is divided into 2 parts extract cocoa peel extract 6% and NaOCl 5%, then each petridish given a paper disc that has been poured 10μl by each material, then incubated inthe incubator for 2x24 hours at 37oC and observe the diameter of the inhibition zone formed using a caliper. Results: The average diameter of the inhibition zone formed in the 6% cocoa peel extract was 11.8375 mm and NaOCl 5% was 26.0344 mm against Lactobacillus acidophilus. Conclusion: There is a difference in antibacterial power between 6% cocoa peel extract and 5% NaOCl against Lactobacillus acidophilus.

Co-Authors Adityatama, Anneke Paramita Agung Sosiawan Ahmad Yudianto Ambarwati, Venny Lusanda Amit Bhardwaj Ananta Tantri Budi Andi Syahrimayani Andi Syahrimayani Anuj Bhardwaj Ari Subiyanto Ari Subiyanto, Ari Arif Rahman Nurdianto Arif Rahman Nurdianto Budiharjo, Adrianto Chang, Nicole Ava Chusnita, Ria Cornelia Melinda Adi Santoso Dalhar Hakiki Darmawan Darwis Devi Eka Juniarti Dwikora Novembri Utomo Dyanita Hasri Eric Priyo Prasetyo Eveline Yulia Darmadi Evelyn Tjendronegoro Evelyn Tjendronegoro Fadiyan Amriel, Menza Febriastuti Cahyani Fery Setiawan Firmansyah, Awang Bagus Galih Sampoerno Goenharto, Aurelia Gunawan, Nathania Elita Handoko, Jessica Cristy Heni Puspitasari Ira Widjiastuti Ivon Dewi Setianingrum Karlina Samadi Ketut Suardita, Ketut Kevin Prayogo Kratika Mishra Kun Ismiyatin Kurniati, Mala Latief Mooduto, Latief M Rulianto Marpaung, Grace Julieta Mega Selvia Monika Werdiningsih Nabiela Rahardia Paidal, Nurfahira Prasita, Shafa R. Roulianto Ria Chusnita Riyadi, Tiara Lathifah Rizka Firdaus Rosidin RM Coen Pramono D Saptaningrum, Mardikaning Riasta Saravanan, Pravinna Setyabudi Setyabudi Setyabudi Setyabudi Setyabudi Setyabudi, Setyabudi Shariz Bin Sharizal, Shafy Sheetal Khandelwal Shivani Bhardwaj Sholeh Ardjanggi Sukandar, Wilson Sukaton, Sukaton Susanto, Dany Agus Sutikno, Antonius Edwin Swadheena Patro Tamara Yuanita Tantri Wismayaning Radito Tjendronegoro, Evelyn Ulfadi , Bakhiitah Thufailah Putri Vera Rimbawani Sushanty Widya Saraswati Wiratno, Amelia Rahma Yemy Ameliana Yessy Andriani Fauziah Yuline Krishartini

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Journal : Conservative Dentistry Journal