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All Journal Narra J Hang Tuah Medical Journal Jurnal Pengabdian Masyarakat Dharma Andalas JPMP Jurnal Widya Medika
Masyeni, Sri
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Correlation of miR-150, hsa-let-7e, and miR-146a and gene expression of IL-6, IL-8, IP-10, and MIP-1β during dengue virus infection Masyeni, Sri; Kuntaman, Kuntaman; Aryati, Aryati; Sofro, Muchlis AU; Hadi, Usman; Mastutik, Gondo; Purnomo, Windu; Santosa, Agus; Yohan, Benediktus; Nelwan, Erni Juwita; Sasmono, R. Tedjo
Narra J Vol. 1 No. 1 (2021): April 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v1i1.31

Abstract

Growing evidence suggests that microRNAs (miRNAs) play a pivotal role in viral infection. The objective of this study was to assess the association between the expression of miR-150, hsa-let-7e, and miR-146a on cytokine expression during dengue infection. Dengue virus (DENV) strain SJN-006, a serotype 2 DENV strain of the Cosmopolitan genotype, isolated in Bali, Indonesia, was used to infect peripheral blood mononuclear cells (PBMCs) isolated from healthy individuals. The relative gene expressions of miR-150, hsa-let-7e, and miR-146a as well as the gene expression of cytokines (IL-6, IL-8, IP-10, and MIP-1β) were determined using quantitative real time - polymerase chain reaction (qRT-PCR) at 6, 12 and 24 hours post infection (hpi). Correlations between the microRNAs and cytokines were analyzed by means of causality tests. Our data suggests that miR-150 and hsa-let-7e were significantly higher in infected-PBMCs after 12 hpi compared to the uninfected-PBMCs (p<0.05). The causality tests demonstrated that miR-150 and hsa-let-7e were negatively correlated with IL-8 expression, meanwhile miR-146a was the contrast. DENV infection was negatively and positively correlated with miR-150 and hsa-let-7e, respectively, after 24 hpi. In conclusion, our data demonstrates the vital role of miR-150, hsa-let-7e, and miR-146a in regulating IL-8 expression with possible different pathways.
Persistence of long COVID symptoms in COVID-19 survivors worldwide and its potential pathogenesis - A systematic review and meta-analysis Fahriani, Marhami; Ilmawan, Muhammad; Fajar, Jonny K.; Maliga, Helnida A.; Frediansyah, Andri; Masyeni, Sri; Yusuf, Hanifah; Nainu, Firzan; Rosiello, Francesco; Sirinam, Salin; Keam, Synat; Ophinni, Youdiil
Narra J Vol. 1 No. 2 (2021): August 2021
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narraj.v1i2.36

Abstract

The study sought to determine the prevalence of persistent long COVID symptoms such as anxiety, depression, dizziness, chest pain, sleep difficulty, palpitations, weight loss, and hair loss among coronavirus disease 2019 (COVID-19) survivors worldwide and to discuss the potential pathogeneses. Potential studies were searched in three databases (PubMed, Scopus, and Web of Science) as of January 30, 2021. Data on study characteristics, patient characteristics during the follow-up, the number of patients with persistent long COVID symptoms and total COVID-19 survivors were collected according to PRISMA guidelines. To assess the quality of studies, the Newcastle-Ottawa scale was used. The estimated prevalence of each long COVID symptom and the association between COVID-19 severity and the occurrence of prolonged symptoms was assessed, if appropriate. The global prevalence of prolonged anxiety was 15.76% (95%CI: 6.36%, 25.15%). Chest pain persisted in 10.36% (239/3,224) of COVID-19 patients (95%CI: 4.92%, 15.80%). Prolonged depression was found in 24 of 548 COVID-19 survivors with an estimated prevalence of 4.32% (95%CI: 2.62%, 6.03%) and dizziness was presented in 4.83% (118/2,219, 95%CI: 1.50%, 8.16%) after recovery. Hair loss was complained by 527 of 2,251 recovered patients (cumulative prevalence of 24.76%, 95%CI: 19.60%, 29.91%), while weight loss was identified in 37 cases among 452 COVID-19 survivors (8.19%, 95%CI: 5.66%, 10.71%). Prolonged palpitation was experienced by 19.38% (211/1,926) survivors with 95%CI: 2.40%, 41.16%. Sleep difficulty was found in 541 of 2,622 COVID-19 survivors (17.87%, 95%CI: 7.55%, 28.20%). The association between COVID-19 severity and the occurrence of persistent long COVID symptoms was not analyzed due to the lack of data. In conclusion, persistent psychological symptoms are frequently reported among COVID-19 survivors. Follow-up studies with a longer duration and larger population are warranted to assess the extent of prolonged symptoms and the quality of life of COVID-19 survivors. Despite various potential pathogeneses that have been hypothesized, a definitive mechanism is yet to be addressed.
Titer disparity of anti-Spike receptor binding domain SARS-CoV-2 antibody between vaccinated and naturally infected individuals Surawan, Dewa P.; Sumohadi, Duwi; Budhitresna, Anak AG.; Lestari, Putri P.; Dewi, Kartika; Wikananda, Wasudewa; Suwari, Retenra P.; Islam, Md. Rabiul; Te, Haypheng; Rabaan, Ali A.; Masyeni, Sri
Narra J Vol. 2 No. 1 (2022): April 2022
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v2i1.71

Abstract

In conjunction with other health promotion strategies, vaccination of coronavirus disease 2019 (COVID-19) is a strategy to alleviate the burden of infection. The aim of this study was to determine the differences in antibody response strength between individuals who received COVID-19 vaccination and those who had a natural infection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A cross-sectional study was conducted among post-natural confirmed COVID-19 infection and immunized people in Bali, Indonesia. The vaccination was using Sinovac-CoronaVac with two-weeks interval between the two vaccine doses. To measure the level of anti-Spike receptor binding domain (SRBD) of SARS-CoV-2 antibody, we used Roche electro-chemiluminescence immunoassay (ECLIA) platform. Blood samples were obtained before and 28 days after first immunization in the vaccinated group, as well as two weeks after hospital discharge in the confirmed COVID-19 patients based on real-time reverse transcription polymerase chain reaction (RT-PCR). A total of 58 confirmed COVID-19 patients and 60 vaccinated individuals were included. On the 28th day after the initial vaccination, the seroconversion rate among vaccinated individuals was 91.67%. The mean titer of anti-SRBD SARS-CoV-2 antibody among vaccinated participants was 63.62±82.57 IU/mL (ranged between 0 IU/mL and 250 IU/mL). The mean titer among naturally infected group was 188.47±94.57 IU/mL (ranged between 4.25 IU/mL to 250 IU/mL) regardless the severity of COVID-19. Our data suggested that the titer of anti-SRBD SARS-CoV-2 antibody was significantly higher in naturally infected individuals compared to those who received COVID-19 vaccination (p<0.001). These data suggest that not all individuals vaccinated with Sinovac COVID-19 had protective level of anti-SRBD SARS-CoV-2 antibody and booster dose of heterologous vaccine maybe required.
The role of mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) on cytokine production during dengue virus infection Masyeni, Sri; Kuntaman, Kuntaman; Aryati , Aryati; Sofro, Muchlis AU.; Hadi, Usman; Mastutik, Gondo; Purnomo, Windu; Santosa, Agus; Iqhrammullah, Muhammad; Yohan, Benediktus; Nelwan, Erni J.; Sasmono, R. Tedjo
Narra J Vol. 3 No. 2 (2023): August 2023
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v3i2.167

Abstract

Inability to understand the pathogenesis of severe dengue, in particular the control mechanism of immune responses, has led to high mortality rate for patients with dengue shock syndrome (DSS). The aim of this study was to determine the control mechanism of cytokine production by mediator suppressor of cytokine signaling (SOCS), toll-like receptor 3 (TLR-3) and nuclear factor kappa B (NFκB) during DENV infection. Peripheral blood mononuclear blood cells (PBMC), isolated from healthy individuals, were infected with dengue virus (DENV)-2 strain SJN-006 Cosmopolitan genotype (isolated from Bali, Indonesia). The relative gene expression of SOCS-3, TLR-3, NFκB, and the cytokine genes (interleukin (IL)-6, IL-8, interferon inducible protein 10 (IP-10), and macrophage inflammatory protein-1 beta (MIP-1β)) were measured using qRT-PCR at 6, 12 and 24 hours post infection (hpi). Student t-test and Mann-Whitney test were used to compare the gene expressions while causal correlations were analyzed using regression test and path analyses. DENV-2 infection increased the gene expression of SOCS-3, TLR-3, and NFκB after 12 and 24 hpi. The expression of IL-6, IL-8, IP-10, and MIP-1β genes was increased and peaked at different times post-infection. NFκB and SOCS-3 genes likely have role in the upregulation of IL-8 and IL-6 gene expression, respectively. MIP-1β gene expression was significantly induced by both NFκB and SOCS-3. In conclusion, our study suggested that SOCS-3, TLR-3, and NFκB are important in regulating the production of IL-6, IL-8, IP-10, MIP-1β during early phase of DENV-2 infection. This enriches our understanding on pathogenesis pathway of DENV-associated cytokine storm.
Relationship between neutrophil-lymphocyte ratio and platelet-lymphocyte ratio with the severity of COVID-19 Arini, Ida A.; Masyeni, Sri; Widhidewi, Ni W.
Narra J Vol. 4 No. 1 (2024): April 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i1.262

Abstract

Coronavirus disease 2019 (COVID-19) is an infectious disease that spreads rapidly causing a high case fatality rate in vulnerable populations. Neutrophil-lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) are known to be inflammatory biomarkers in certain infections. The aim of this study was to determine the relationship between NLR and PLR with the severity of COVID-19. A cross-sectional study was conducted at Tabanan Regency General Hospital, Bali, Indonesia, from January 2021 to December 2022. All patients included in the study tested positive for COVID-19 by real-time polymerase chain reaction (RT-PCR), aged 18–50 years with no comorbid. Laboratory examinations were carried out on admission. The patients were categorized into two groups based on the severity: moderate and severe/critical. The Mann-Whitney test was used to determine the association between NLR and PLR with the severity of COVID-19. A total of 104 patients were included in the study, the majority of COVID-19 patients had moderate (77.9%) severity. The average NLR was 5.8 and the PLR was 21.7. There was a significant relationship between NLR (p=0.002) and PLR (p=0.001) with the severity of COVID-19. The defined cut-off values of NLR and PLR were ≥3.8 and ≥106, yielding sensitivities of 95% and 70%, and specificities of 74% and 50%, respectively. This study highlights the promising role of NLR and PLR as predictive biomarkers to assess COVID-19 severity.
Cytokine profiles in dengue fever and dengue hemorrhagic fever: A study from Indonesia Masyeni, Sri; Wardhana, I Made W.; Nainu, Firzan
Narra J Vol. 4 No. 1 (2024): April 2024
Publisher : Narra Sains Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52225/narra.v4i1.309

Abstract

Recent studies have demonstrated that cytokine dysregulation has a critical role in the pathogenesis of dengue hemorrhagic fever (DHF) and dengue shock syndrome (DSS). The aim of this study was to investigate the association between tumor necrosis factor (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), and interleukin 17 (IL-17) with infection status, and severity of dengue. A prospective cross-sectional study was conducted at three hospitals in Gianyar regency and Denpasar municipality, Bali, Indonesia, from June to December 2022. Sixty-four dengue infected patients were involved. Patients’ serum was tested for dengue infection using NS1 antigen rapid test, dengue virus immunoglobulin M (IgM) and immunoglobulin G (IgG) test, and reverse transcription polymerase chain reaction (RT-PCR). Cytokine levels (TNF-α, IL-6, IL-10, and IL-17) were measured using enzyme-linked immunosorbent assay (ELISA). Infection status was determined by combining serological and RT-PCR results, categorizing patients into primary and secondary infections. The present study found that DF patients had lower TNF-α, IL-6, and IL-17 but higher IL-10 levels compared to DHF patients (p<0.001). Elevated TNF-α, IL-6, and IL-17 levels were higher in secondary infection, while IL-10 level was higher in primary infection (p<0.001). In conclusion, cytokines play a crucial role in the interplay between cytokine dysregulation and dengue infection dynamics.
Karakteristik Pasien Penderita Apendisitis Akut di RSUD Sanjwiani, Gianyar: Apendisitis akut MASYENI, SRI; SANJAYA LIE, MIRACLE NYOMAN; WIJAYA, INDRA
Hang Tuah Medical Journal Vol 22 No 1 (2024): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/htmj.v22i1.661

Abstract

Acute appendicitis is one of the most common emergency surgical cases of lower right abdominal pain, a small proportion of cases in emergency units in the world which are often experienced by children and adults. This study aims to identify the characteristics of acute appendicitis in patients at Sanjiwani Hospital, Gianyar. This research is a descriptive study with a retrospective cross-sectional approach method that uses secondary data in the form of medical records of acute appendicitis patients who underwent examinations at Sanjiwani Regional Hospital, Gianyar in the period 1 January 2020–31 December 2022. The results showed that the highest number of acute appendicitis sufferers were aged 10–19 years (34.3%), with the highest incidence in males (60.6%), and the most symptoms found, namely lower right abdominal pain (100%), with the most positive examination of the Mc Burney Sign (89.9%) and increased white blood cells with an average of 12.8 x 10^3/uL.
Karakteristik Pasien Dengan Penyakit Ginjal Kronis Stadium V Yang Menjalani Hemodialisis Reguler Di Rumah Sakit Tabanan MASYENI, SRI; MADE , SUHITA GIANI; PUTU, ARYA SURYANDITHA
Hang Tuah Medical Journal Vol 22 No 2 (2025): Hang Tuah Medical Journal
Publisher : Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/htmj.v22i2.810

Abstract

Abstract Chronic Kidney Disease (CKD) significantly impacts global health, contributing to both morbidity and mortality. This condition is characterized by severe kidney damage, which results in increased blood urea levels and can lead to serious complications. Reports indicate that the number of CKD cases is on the rise. This study is descriptive and employs a cross-sectional design, analyzing the medical records of CKD patients. The focus is on individuals who underwent examinations at Tabanan Hospital between January 1, 2019, and May 31, 2024. A total of 101 subjects were selected for the analysis. The results revealed that the majority of patients with stage V chronic kidney failure were over 60 years old (38.6%). The highest incidence of CKD was observed in men, accounting for 53.5% of the cases. Most patients had hypertension that was managed with medication (67.3%), and approximately 50.5% of the subjects experienced severe anemia
PEMBERDAYAAN KARYAWAN HOTEL FOURTEEN ROSES LEGIAN, DALAM PENCEGAHAN PENYAKIT INFEKSI SALURAN NAFAS BAWAH DALAM UPAYA PENCAPAIAN SUSTAINABLE DEVELOPMENT GOALS KETIGA Masyeni, Sri; Permana Lestari, Sagung Putri; Wedari, Niti
Jurnal Pengabdian Masyarakat Pesisir VOLUME 4 NOMOR 1
Publisher : Universitas Hang Tuah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/jpmp.v4i1.133

Abstract

The threat of various infectious diseases remains emerge in the world after COVID-19 pandemic. Public health response should be addressed to combating the infectious diseases threat. The community services are an important activity to achieve the third SDGs programme such as the prevention the lower tractus respiratory infection. This community partner was the Fourteen Roses Beach Hotel staff. The activities were training and counselling about the lower tractus respiratory infection. We report an increasing of the partners knowledge of prevention the lower tractus respiratory infection as of 70%. The current community service results will support the achievement of the third SDGs
Pengentasan Stunting di Lombok Barat Untuk mencapai Sustainable Development Goals Ketiga Masyeni, Sri; Yuniekawati, Yuyun; Lestarini, Ima Arum; Kadriyan, Hamsu; Sahadewa, Sukma; Rismawaty, Ika; Pinatih, Januartha Putra; Asparini, Ruby Riana
Jurnal Pengabdian Masyarakat Dharma Andalas Vol 4 No 1 (2025): Jurnal Pengabdian Masyarakat Dharma Andalas
Publisher : LPPM Universitas Dharma Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.47233/jpmda.v3i2.1897

Abstract

Background: Stunting, a global issue, is being addressed by the World SDGs program, which has identified stunting alleviation as a key focus. Objectif: To enhance partner understanding of stunting, its treatment, and prevention. Methods: The community service method involves socialization through focus group discussions, counseling, and evaluation. Results: 100% of partners demonstrated an increase in their knowledge about stunting, with the average pretest score at 27 and the average posttest score at 83. Conclusion: Counseling, a practical and effective tool, significantly enhances knowledge about stunting.
Co-Authors Agus Santosa Andri Frediansyah, Andri Arini, Ida A. Aryati , Aryati Aryati Aryati Budhitresna, Anak AG. Erni J. Nelwan, Erni J. Erni Juwita Nelwan, Erni Juwita Fahriani, Marhami Firzan Nainu Gondo Mastutik Hanifah Yusuf I Made W. Wardhana Ilmawan, Muhammad Ima Arum Lestarini Iqhrammullah, Muhammad Jonny K. Fajar Kadriyan, Hamsu Kartika Dewi Keam, Synat Kuntaman Kuntaman Lestari, Putri P. MADE , SUHITA GIANI Maharani, Ni Luh Putu Silfia Maliga, Helnida A. Md. Rabiul Islam Ophinni, Youdiil Paramasatiari, Anak Agung Ayu Lila Permana Lestari, Sagung Putri Pinatih, Januartha Putra Purnomo, Windu PUTU, ARYA SURYANDITHA Rabaan, Ali A. Rismawaty, Ika Rosiello, Francesco Ruby Riana Asparini SANJAYA LIE, MIRACLE NYOMAN Sasmono, R. Tedjo Sirinam, Salin Sofro, Muchlis AU Sofro, Muchlis AU. Sukma Sahadewa, Sukma Sumohadi, Duwi Surawan, Dewa P. Suwari, Retenra P. Te, Haypheng Trisnia, Putu Ayunda Usman Hadi Wedari, Niti Widhidewi, Ni W. Wijaya, Indra Wikananda, Wasudewa Yohan, Benediktus Yuniekawati, Yuyun