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Anti-glucosidase Activity and Antioxidant Capacity of Premna serratifolia from Sintang, Kapuas Hulu, and Sambas District, West Kalimantan, Indonesia Hadiarti, Dini; Sunandar, Ari; Cipta, Indra; Kurniawan, Rizmahardian Ashari
HAYATI Journal of Biosciences Vol. 33 No. 1 (2026): January 2026
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.33.1.153-166

Abstract

The secondary metabolites in plants such as Premna serratifolia (P. serratifolia) are influenced by their growing locations, which in turn affects their bioactivity. This study aimed to compare the antioxidant activity and α-glucosidase inhibition of P. serratifolia leaf extracts from the Sintang, Kapuas Hulu, and Sambas districts in West Kalimantan. Antioxidant capacity was evaluated using the DPPH method, while the reduction of α-glucosidase activity was assessed using the PNPG method. Functional groups and active compounds were identified using FTIR and UHPLC-Q-Orbitrap HRMS. The findings indicated that the extract from Kapuas Hulu, obtained through maceration (KHM), exhibited the highest antioxidant activity (IC₅₀ 18.39) and contained the most total phenolic content (TPC). The best α-glucosidase inhibition activity (IC₅₀ 4194.14) was found in the extract from Sambas obtained through Soxhlet extraction (SSI), which had the highest total flavonoid content (TFC). Principal Component Analysis (PCA) revealed that the functional groups and active compounds of P. serratifolia from Kapuas Hulu and Sintang were similar, as both regions are located in eastern West Kalimantan. Additionally, Partial Least Squares (PLS) analysis revealed that the C-H alkane, O-H, and isoferulic acid were the most influential compounds in determining antioxidant activity. At the same time, the C=O group and NP-000308 predominantly influenced α-glucosidase inhibition.
Activated Carbon from Mulu Bebe Stem Waste for Methylene Blue (MB) Adsorption Cipta, Indra; Baturante, Nur Jannah; Zam Zam, Zulkifli; Pasimanyeku, Angela; Rombe, Yunita Pare
TECHNO: JURNAL PENELITIAN Vol 13, No 1 (2024): TECHNO JURNAL PENELITIAN
Publisher : Universitas Khairun

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33387/tjp.v13i1.6862

Abstract

Methylene blue is a harmful synthetic dye waste component that poses a significant threat to the environment and human health if not disposed of properly. The best way to tackle this problem is by adsorption through activated carbon from Mulu Bebe banana stems, which are widely available in North Maluku. This agricultural waste can be used to produce activated carbon through pyrolysis at 500ËšC for 30 minutes and activation using 0.5 M KOH solution for 24 hours. The activated carbon is then characterized using FTIR, SEM and XRD, which reveal the presence of various functional groups and pores. The XRD results indicate that the activated carbon structure tends to be amorphous. The adsorption analysis shows that the activated carbon from Mulu Bebe banana stems has a high adsorption capacity of 12.4 mg/g at the optimum condition of pH 7, contact time of 30 minutes, and initial concentration of 50 ppm. The Langmuir and Freundlich equations provide the best fit for the equilibrium adsorption data. By using activated carbon from Mulu Bebe banana stems, we can effectively reduce the amount of methylene blue waste in the environment and protect the ecosystem and human health.
Carbon Electrode and Buleleng Seawater in Degrading the Leachate in Bengkala Village of Buleleng Regency by Using Electro-Oxidation Techniques Wiratini, Ni Made; Lasia, I Ketut; Rakhman, Khusna Arif; Cipta, Indra
Molekul Vol 21 No 1 (2026)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jm.2026.21.1.12130

Abstract

ABSTRACT. This research aims to study the effectiveness of using carbon electrodes and Buleleng seawater in degrading leachate through electro-oxidation techniques. Electro-oxidation is a waste processing technology that cannot be biologically degraded, has high efficiency and degradation speed, is easy to operate, has low cost, and is environmentally friendly. Leachate is taken from the dumping ground in Bengkala village of Buleleng Regency. The volume of leachate used was 50 mL. The influence of the surface area of the electrode, the distance between electrodes, voltage, contact time, and the amount of seawater were also studied. All treated leachates were then measured for COD, DO, absorbance, BOD, and pH. The results showed that Buleleng sea water contains chlorine ions 16.862 g/L. COD, DO, absorbance, BOD, and pH of leachate before electro-oxidation 38.763x103, 0, 17.1, 0, and 9 mg/L respectively. The surface area of the electrodes, the distance between electrodes, voltage, contact time, and the amount of seawater used were most effective in degrading leachate in 10.551 cm2, 3 cm, 13.5 volts, 1.5 hours, and 560 mL respectively. Keywords: Buleleng seawater, carbon electrodes, degrading leachate, electro-oxidation technique