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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Jurnal Ilmiah Inovasi Jurnal Biosense Jurnal Riset Perkebunan (JRP) RADIKULA: Jurnal Ilmu Pertanian International Journal of Technology, Food and Agriculture Agrimas : Jurnal Pengabdian Masyarakat Bidang Pertanian Jurnal Pengabdian Masyarakat Berdampak Jagad Tani: Jurnal Ilmu Pertanian diterbitkan
Setyoko, Ujang
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Other

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Embryogenic Callus Regeneration Response of Several Arabica Coffee Varieties under Drought Stress Conditions Using Polyethylene Glycol: Callus Regeneration of Arabica Coffee Under PEG Stress Asmono, Sepdian Luri; Rahmawati, Rahmawati; Arifiana, Nisa Budi; Setyoko, Ujang
Journal of Tropical Life Science Vol. 16 No. 2 (2026)
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/jtls.16.02.09

Abstract

This study aimed to evaluate the effect of polyethylene glycol (PEG) on the growth and development of primary embryogenic callus in four Arabica coffee varieties (Gayo, Orange Bourbon, Andungsari 1, and Lini S-795). The research was conducted at the Tissue Culture Laboratory, Politeknik Negeri Jember, using a factorial completely randomized design with two factors, namely coffee variety and PEG concentrations of 0, 5, 7.5, and 10%. A total of 16 treatment combinations were replicated four times, producing 64 experimental units, each consisting of one bottle containing a single primary callus clump. Subsequently, 3-month-old primary calli were cultured on modified MS medium supplemented with Vitamin B5 and different PEG concentrations. The evaluated parameters included Proembryogenic Mass (PEM) formation percentage, emergence time, clump weight, and regeneration capacity of PEM. The results showed that variety and PEG concentration independently had a highly significant effect on PEM formation. Gayo produced the highest formation (68.75%), while 5% PEG had 87.5%, compared with 0%, 7.5%, and 10%. The variety × PEG interaction significantly affected PEM emergence time and clump weight. The earliest formation occurred in Orange Bourbon cultured with 5% PEG (27.13 DAP), while the heaviest clumps were obtained from Gayo cultured with 5% PEG (0.115 g). During regeneration, Orange Bourbon showed the highest somatic embryo regeneration rate (90%).
Co-Authors Amanda, Zenitasari Anindita, Devina Cinantya Annisa Lutfi Alwi Arthamurti, Tariza Tiara Ayu Agustin Azkiya, Khoirul Azka Cinantya, Devina Descha Giatri Cahyaningrum Dinata, Gallyndra Fatkhu Eva Rosdiana Fadillah, Anggita Rizky Fandyka Yufriza Ali Fernando, Moch Ricko Hartatie, Dian Hudori, Huda Ahmad Irawan, Triono Bambang Jayana Erliana Kusuma, Satria Indra Kusumaningtyas, Rizky Nirmala Nia Safitiri, Alfina Khusnun Nisa Budi Arifiana Olivia, Zora Rahmawati, Rahmawati Ramadhani, Aditya Dwi Rosdiana, Eva Rudi Wardana Setyo Andi Nugroho Wahyu Tri Handayani, Wahyu Tri Wardana , Rudi Wilujeng, Elly Daru Ika