Article Per Year (5 Year)
p-Index From 2021 - 2026
3.558
P-Index
This Author published in this journals
All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Meditory : The Journal of Medical Laboratory Klinikal Sains (Jurnal Analis Kesehatan) Jurnal Ilmu Kedokteran dan Kesehatan Jambura Journal of Health Sciences and Research Jurnal Medika Malahayati Husada Mahakam: Jurnal Kesehatan JURNAL ANALIS LABORATORIUM MEDIK Borneo Journal of Medical Laboratory Technology Medicra (Journal of Medical Laboratory Science/Technology) Health Care : Jurnal Kesehatan Jurnal Kesehatan dan Pembangunan Jurnal Kesehatan Tambusai Jurnal Sains dan Kesehatan Borneo Journal of Science and Mathematics Education Journal of Sustainability Science and Technology Gema Kesehatan
Kesuma, Suryanata
Poltekkes Kemenkes Kalimantan Timur
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Dentistry Education Energy Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health

Published : 26 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 26 Documents
Search

 1   2   3 
Profil Bakteri Patogen dan Antibiotik pada Gangren Diabetes Melitus di RSUD Abdul Wahab Sjahranie Samarinda Desi Wahyuni; Suryanata Kesuma; Sresta Azahra
HEALTH CARE: JURNAL KESEHATAN Vol 12 No 1 (2023): Health Care : Jurnal Kesehatan
Publisher : STIKes Payung Negeri Pekanbaru

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36763/healthcare.v12i1.370

Abstract

Diabetes Mellitus can be triggered by environmental factors and unhealthy lifestyles such as overeating, fat, lack of activity, and stress. Diabetes Mellitus can also appear due to hereditary factors. Diabetes Mellitus can cause various complications including gangrenous wounds. To determine the appropriate use of antibiotics, appropriate treatment, and the spread of antibiotic resistance does not occur in patients with gangrene with Diabetes Mellitus, it is necessary to identify the appropriate bacterial species. The study aimed to identify the bacterial species found in wound infections in diabetic with gangrenous complications at AWS Hospital. This type of research is descriptive with exploratory design. The research sample was pus (pus) with gangrene in inpatient diabetic in November - December at AWS Hospital. A total of 29 samples were found in pus gangrenous DM wound infection, there were 11 species of gram-negative and gram-positive pathogenic bacteria. Antibiotic sensitivity test on the Staphylococcus epidermidis bacterial species was more resistant to antibiotics by 81%, the Proteus mirabilis bacterial species was more Intermediate against antibiotics by 65%, and the Proteus hauseri bacterial species was more sensitive to bacteria by 66%. The most infecting pathogenic bacteria are gram-negative bacteria with the species Proteus mirabilis and the most resistant and sensitive antibiotics are Ceftizoxime, gentamicin, and Piperacillin/Tazobactam.
Analisis Pemeriksaan Bilirubin Total Berdasarkan Variasi Waktu Paparan Cahaya Lampu pada Spesimen Dwi Wulan Sari; Suryanata Kesuma; Nursalinda Kusumawati
Jurnal Sains & Kesehatan Vol 2 No 2 (2023): Jurnal Sains dan Kesehatan
Publisher : Politeknik Baubau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57151/jsika.v2i2.265

Abstract

Pre-exam stage one of the most important stages in a laboratory examination which includes patient preparation, specimen reception, specimen processing, identification of specimens and preparation of reagents to be used. Specimens that are not immediately examined can cause the results of the total bilirubin examination to become unstable due to exposure to light which affects the properties of the bilirubin so that the concentration of bilirubin levels in the serum decreases. The purpose of this study was to determine the results of total bilirubin examination based on variations in exposure time to light on the specimens. This research isstudy true experimentby designrandomized pretest-posttest control groupwith 3 treatments, namely exposure to laboratory light on serum specimens for total bilirubin examination (2, 4, and 24 hours). This research was conducted in the hematology and clinical chemistry laboratories, medical laboratory technology department at the East Kalimantan Ministry of Health Polytechnic with a total sample of 40 people. The population in this study were students majoring in medical laboratory technology at the East Kalimantan Ministry of Health Polytechnic. The measurement method used in this research is methodEndPointData analysis used isOne Way ANOVA. The results obtained were clinically and statistically different in the ANOVA test which showed that exposure to light at various times of 2, 4, and 24 hours had a significant effect.
PROFIL BAKTERI PENGINFEKSI PUS PADA LUKA DI LABORATORIUM MIKROBIOLOGI RSUD ABDOEL WAHAB SJAHRANIE PERIODE BULAN JANUARI-JUNI TAHUN 2023 Suryanata Kesuma; Maulida Julia Saputri; Patricia Aleksandra
Jurnal Kesehatan Tambusai Vol. 4 No. 4 (2023): DESEMBER 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v4i4.20623

Abstract

Pus adalah salah satu respon tubuh terhadap infeksi yang di mana pus adalah cairan yang mengandung protein dari hasil proses inflamasi yang terbentuk dari sel leukosit, cairan jaringan serta debris selulerPenanganannya adalah dengan melakukan pemberian antibiotik. Tujuan penelitian ini adalah untuk mengetahui profil bakteri yang menginfeksi pus pada luka. Penelitian ini menggunakan penelitian deskriptif dengan pendekatan retrospektif. Dari 120 sampel tersebut, terdapat 17 jenis bakteri dimana 11 diantaranya merupakan gram negatif dan 6 sisanya adalah gram positif, diantaranya Escherichia coli (24,6%), Staphylococcus aureus (20,5 %), Klebsiella pneumoniae (16,4%), Proteus mirabilis (14,8%), Pseudomonas aeruginosa  (4,1%),  Enterococcus faecalis (3,3%), Morganella morganii (2,5 %), Staphylococcus haemolyticus (2,5%), Staphylococcus epidermidis (1,6 %),  Enterobacter cloacae (1,6 %), Streptococcus anginosus (1,6%), Acinetobacter haemolyticus (0,8%), Aeromonas hydophila (0,8%), Aeromonas punctata (0,8%), Acinetobacter baumannii (0,8%), Pseudomonas luteola (0,8 %), Streptococcus agalactia (0,8 %). Spesies bakteri yang dominan ditemukan adalah Escherichia coli (24,6%), Staphylococcus aureus (20,5 %), dan Klebsiella pneumoniae (16,4 %). Diagnosis klinis paling banyak ditemukan pada penderita Ulkus Dekubitus sebanyak 15 pasien, penderita DM type II sebanyak 13 pasien, dan penderita Abses Pedis sebanyak 10 pasien. Bakteri yang paling resisten terhadap antibiotik adalah Bakteri yang paling resisten terhadap antibiotik adalah speises bakteri Acinetobacter baumannii lebih resisten terhadap antibiotik sebesar 92%, spesies bakteri Klebsiella pneumoniae  intermediet antibiotik sebesar 47%, dan spesies bakteri Streptococcus agalactiae lebih sensitif terhadap antibiotik sebesar 100%.
STABILITAS REAGEN KERJA TERHADAP AKTIVITAS ENZIM ASPARTATE AMINOTRANSFERASE (AST) Lamri Lamri; Suryanata Kesuma; Armiah Gita Anggraini
Jurnal Kesehatan Tambusai Vol. 4 No. 4 (2023): DESEMBER 2023
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jkt.v4i4.19152

Abstract

Stabilitas dapat diartikan bahwa suatu produk reagen yang disimpan pada kondisi tertentu didalam kemasan penyimpanan dan pengangkutan tidak mengalami perubahan dalam batas yang diperbolehkan. Stabilitas suatu reagen penting untuk diketahui agar disaat melakukan pemeriksaan didapatkan hasil yang akurat. Pada beberapa laboratorium preparasi reagen masih dilakukan secara manual sehingga pembuatannya melebihi kebutuhan, namun tidak setiap hari suatu laboratorium melakukan pemeriksaan sehingga penyimpanan reagen memerlukan waktu yang cukup lama. Tujuan penelitian ini untuk mengevaluasi penggunaan reagen kerja yang segera digunakan dan disimpan selama 10 hari dan 12 hari pada suhu 2-8°C menggunakan sampel bahan kontrol normal AST. Penelitian ini merupakan penelitian eksperimen kuasi dengan rancangan Posstest Only Control Group Design. Sampel penelitian ini adalah reagen kerja yang dibaca pada bahan kontrol normal sebanyak 30 pemeriksaan. Data yang dikumpulkan dianalisis perbedaan statistik regresi linear kemudian dilanjutkan dengan perhitungan dasar pemantapan mutu. Hasil penelitian yang didapatkan rata rata hasil pemeriksaan aktivitas enzim AST menggunakan  reagen kerja segera digunakan dan reagen kerja setelah disimpan selama 10 dan 12 hari adalah 43,8 U/L, 45,1 U/L, 45,4 U/L dan Persentase selisih kerja segera  digunakan dan reagen kerja setelah disimpan selama 10 dan 12 hari adalah 2,97% dan 3,65%. Nilai akurasi 1,2% pada hari ke 10 dan 1,5% pada hari ke 12. Nilai Presisi 5,0% pada hari ke 10 dan 6,4% pada hari ke 12. Total Error 11,3% pada hari ke 10 dan 14,3% pada hari ke 12. Beradasarkan hasil tersebut reagen kerja yang di simpan selama 10 hari dan 12 hari masih dalam kondisi yang dapat digunakan.
PENGARUH VARIASI WAKTU PENYIMPANAN TERHADAP STABILITAS REAGEN KERJA PADA HASIL PEMERIKSAAN KADAR KREATININ Silvi Marshela; Suryanata Kesuma; Suparno Putera Makkadafi
Klinikal Sains : Jurnal Analis Kesehatan Vol 11 No 2 (2023): Desember
Publisher : LPPM Universitas Abdurrab

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36341/klinikal_sains.v11i2.3809

Abstract

Kidney is one of the organs of the body whose role is very important to maintain the stability of volume, osmolarity of extracellular fluid, and electrolyte composition. One of the parameters to determine kidney function that is usually done is creatinine examination. Preparation of working reagents is very important for accurate results in an examination. The purpose of this study was to determine the effect of storage on the stability of working reagents on creatinine examination. This type of research is a pseudo-experiment, to determine the difference between working reagents that are directly used with working reagents that are stored for several days. The research variables are the length of storage of work reagents, creatinine levels and storage temperature. The sample used in this study was normal creatinine control. The results were analyzed using Microsoft Excel and using the Kruskal Wallis Non Parametric test. The results of the study showed that the average reagent immediately and 5 days was 0.8 mg/dl and at 8 days 1.2 mg/dl, the accuracy obtained was -3.2% and 0.4%, the precision value obtained was 35.1% and 31.0%, in statistical tests significant results <0.05, meaning that there are differences in the results of examinations using working reagents that are used immediately and those stored for 5 and 8 days.
GAMBARAN HASIL PEMERIKSAAN LAKTAT DEHIDROGENASE (LDH) BERDASARKAN LEVEL HEMOLISIS Hikmah, Nurul; Irawiraman, Hadi; Kesuma, Suryanata
Jurnal Medika Malahayati Vol 8, No 1 (2024): Volume 8 Nomor 1
Publisher : Universitas Malahayati

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33024/jmm.v8i1.13369

Abstract

Abstrak: Gambaran  Hasil Pemeriksaan Laktat Dehidrogenase (LDH) Berdasarkan Level Hemolisis. Pemeriksaan kadar Laktat Dehdirogenase dalam darah merupakan parameter yang terdapat pada jantung, otot rangka dan lainnya. Sampel plasma atau serum untuk aktivitas lakta dehidrogenase dapat menyebabkan sampel mengalami hemolisis. Data diperoleh melalui pengukuran terhadap objek penelitian setelah diberikan implementasi berupa penambahan hemolisat berbagai kadar. Analisis data menggunakan Microsoft Excel. Nilai akurasi 0,4% pada pemeriksaan sampel laktat dehidrogenase hemolisis tidak signifikan dan hemolisis ringan adalah 0,2% dan 0,7%. Nilai presisi 7% pada pemeriksaan laktat dehidrogenase sampel hemolisis insignifikan dan hemolisis ringan adalah 55,4% dan 62,7%. Total Error TEa 20% pada pemeriksaan sampel hemolisis tidak signifikan dan hemolisis ringan adalah 531% dan 1637%.
An Overview of Serologic Test Results in Covid-19 Patients: Gambaran Hasil Pemeriksaan Serologi pada Pasien Covid-19 Kesuma, Suryanata
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 7 No. 1 (2024): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v7i1.1682

Abstract

Coronavirus Disease 2019 (Covid-19) is an infectious disease caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). SARS-CoV-2 protein and antibody/serology assays can be used for sero-surveillance studies and analyze the epidemiology and virology of SARS-CoV-2. This study aims to review the results of serological tests in Covid-19 patients with the parameters of IgM and IgG antibodies, C-Reactive Protein, procalcitonin, and serum ferritin through literacy studies. This research is descriptive. The study was conducted by collecting and reviewing data on the results of serological examinations in Covid-19 patients contained in articles and journals. After collecting data, 10 journals were obtained that matched the inclusion criteria set by the researcher. The conclusion of this study was that there was an increase in c-reactive protein and procalcitonin levels in Covid-19 patients, serum ferritin levels were also found to tend to increase. IgM antibodies were found to increase earlier than IgG antibodies, but their levels decreased more quickly. An increase in IgG antibodies occurs around the second week after the onset of symptoms.
GAMBARAN KADAR CRP PADA MENCIT ALERGI SETELAH TREATMENT INFUSA DAUN KETEPENG Firmansyah, Adi; I Gede Andika Sukarya; Suryanata Kesuma
Jurnal Kesehatan dan Pembangunan Vol 14 No 1 (2024): Jurnal Kesehatan dan Pembangunan
Publisher : LPPM STIKes Mitra Adiguna

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52047/jkp.v14i27.269

Abstract

Allergy is a hypersensitivity reaction that arises due to exposure to allergens. Allergens will bind to IgE attached to mast cells and cause these cells to release various chemicals in the blood. One of them is, histamine which causes most allergic reactions. Allergic reactions can cause inflammation or inflammation. Inflammatory stimulation will later stimulate the release of C-Reactive Protein (CRP). CRP is an inflammatory maker that is produced and synthesized in the liver. Ketepeng leaf infusion has the potential to stimulate an immune response because it contains chemical compounds, namely alkaloids, saponins, flavonoids, tannins, and anthracino. Flavonoids contained in this plant function to provide anti-inflammatory, antiallergic, antioxidant and antifungal effects. The purpose of this study was to determine the effectiveness of ketepeng leaves (Cassia alata L) by examining CRP (C-Reactive Protein) levels in Ovalbumin-allergic mice (Mus musculus). This type of research is Univariate, which is a method for making an overview of the state of the object being examined through data or sampling samples. The results of this study found that in group 2 after being given ovalbumin, CRP levels increased by 181.33 mg/L and in group 3, after being given ketepeng leaf infusion, CRP levels decreased by 48 mg/L. The conclusion of this study is that ketepeng leaf infusion can reduce CRP levels in mice with ovalbumin allergy and there is an effect of increasing CRP levels in mice given ovalbumin of 181.33 mg/L and a decrease in CRP levels after the treatment of ketepeng leaf infusion of 48 mg/L .
Evaluasi Pemeriksaan Kreatinin dengan Penundaan 12 Jam pada Pasien Diabetes Melitus Kesuma, suryanata; Eka Farpina, Eka Farpina; Sultan, Sultan
Jurnal Sains & Kesehatan Vol 3 No 1 (2024): Jurnal Sains dan Kesehatan
Publisher : Politeknik Baubau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.57151/jsika.v3i1.264

Abstract

Clinical laboratories must always pay attention to the quality of service and quality of examination. The clinical laboratory will provide information in the form of examination results to clinicians so that they can be used to make a diagnosis and follow up treatment of patients. Clinicians always expect the results of the examination to be precise and accurate according to the patient's condition at that time. Examination results and precise measurements can minimize errors in determining the diagnosis of the patient's disease at that time. The purpose of this study was to find out the clinical differences in delaying immediate and delayed 12-hour creatinine checks in patients with diabetes mellitus. The type of research used in this research is descriptive, the specimens used are serum, the data collection technique uses primary data, Mindray Chemistry Analyzers BS240, data processing in this study uses MicrosoftExcel. The results of this study showed an average of 0.9 mg/dL on immediate examination and a 12-hour delay of 0.6 mg/dL. The accuracy (d%) of the immediate creatinine check against a 12-hour delay was -24.8%. Precision (CV%) on immediate creatinine examination against a 12-hour delay of 20.0% andtotalerror on immediate creatinine examination against a 12- hour delay of 64.8%. Based on these results indicate that there are differences   in   clinical   results   in   the   immediate   creatinine examination and a delay of 12 hours.
Design, Construction and Expression of Spike Highly Conserved Region (HCR) SARS-CoV-2 and Cholera Toxin Subunit B Fusion Protein in Lactococcus lactis NZ3900: Construction of recombinant plasmid in Lactococcus lactis Kesuma, Suryanata; Adianingsih, Oktavia Rahayu; Winarsih, Sri; Widodo, Nashi; Yurina, Valentina
Journal of Tropical Life Science Vol. 15 No. 2 (2025): In Press
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/2xf1qp96

Abstract

Prevention of SARS-CoV-2 transmission has primarily been achieved through vaccination, which is generally administered via injection and may cause discomfort. No commercially available SARS-CoV-2 vaccines can be administered via the mucosal route. However, recent advancements have demonstrated that vaccination with Lactococcus lactis enables vaccine delivery through the mucosa. A promising target for SARS-CoV-2 vaccine materials is the highly conserved region (HCR) of the SARS-CoV-2 spike (SARS-CoV-2 HCR Spike). Vaccine efficacy is enhanced by adding Cholera Toxin Subunit B (CTB) as an adjuvant. HCR and CTB proteins were recombinantly fused using a synthetic gene with optimized codons. This study aimed to construct a fusion protein of the SARS-CoV-2 spike protein and CTB in L. lactis strain NZ3900. The construction and expression of fusion proteins were analyzed using sequencing and protein electrophoresis. Codon optimization resulted in a Codon Adaptation Index value of 0.93 and a GC content of 27.06%. The cloning results revealed the formation of L. lactis colonies expressing the Fusion protein of the SARS-CoV-2 HCR Spike and CTB, which formed yellow colonies on the selection Elicker medium. PCR and sequencing confirmed the presence of the hcr-ctb gene, with a length of 981 bp and 100% sequence similarity. The Fusion protein of the SARS-CoV-2 HCR Spike and CTB was successfully expressed with a molecular weight of >35 kDa. In conclusion, we successfully constructed a Fusion protein of the SARS-CoV-2 HCR Spike and CTB in L. lactis NZ3900 as a potential vaccine candidate for oral administration to prevent SARS-CoV-2 infection.
Co-Authors Adi Firmansyah Agus Rudi Hartono Amalia Diah Suci Rahmah Anggrieni, Nurul Armiah Gita Anggraini DESI WAHYUNI Didi Irwadi Dwi Wulan Sari Eka Farpina, Eka Farpina Elita Octavia Farhan Baehaki Farpina, Eka Fera, Fera Hartono, Agus Rudi I Gede Andika Sukarya Irawiraman, Hadi Lamri Lamri Lamri Lamri Liah Kodariah Mahdiah Syumarliyanty Marezha Aulia Putri Sugiarsono Maulida Maulida Julia Saputri Nanda Nur Adha Sari Febriyanti Nashi Widodo Nia Ardelia Nursalinda Kusumawati Nurul Alidasyah Nurul Hikmah Oktavia Rahayu Adianingsih Patricia Aleksandra Prihandono, Dwi Setiyo Rahmad Rezaldy Sari, Putri Sekar Silvi Marshela Siti Rodiyah, Siti Sresta Azahra Sri Winarsih Sultan Sultan Suparno Putera Makkadafi Suparno Putera Makkadafi Supri Hartini Tatang Abdullah VALENTINA YURINA Widyadari, Effie Raissa