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PRECISION AND ACCURACY OF NORMAL AND ABNORMAL CREATININE LEVELS OF JAFFE METHOD AGAINST ENZYMATIC METHODS Fa'iqotul Azharil Jauharoh; Anik Handayati; Museyaroh; Edy Haryanto
Journal of Vocational Health Studies Vol. 10 No. 1 (2026): July 2026 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V10.I1.2026.56-62

Abstract

Background: The Jaffe method is still applied with semi-automated analyzers based on picric acid reaction in many laboratories, whereas the enzymatic method is more frequently used as a reference in primary laboratories and hospitals. Purpose: To evaluate the accuracy of the Jaffe method in comparison with the enzymatic method and to assess its precision in measuring normal and abnormally high serum creatinine levels under routine laboratory conditions. Method: The study employed an analytical observational manner at the East Java Province Hajj Hospital and Bakti Analysa Laboratory. Serum samples were obtained from 30 patients with normal creatinine levels and 25 pre-dialysis patients with abnormally high creatinine levels. Accuracy was evaluated by comparing the results of the Jaffe method with those obtained using the enzymatic method, while precision was assessed by repeating the measurements 10 times for each sample. Result: The inaccuracy of the Jaffe method for normal creatinine levels was 19.20%, while for abnormally high creatinine levels, it was -9.33%. The precision value (coefficient of variation) for normal creatinine levels was 5.86%, whereas for abnormally high creatinine levels, it was 1.13%. Conclusion: The Jaffe method for measuring normal creatinine levels demonstrated poor accuracy because the inaccuracy value exceeded the ±10% limit. In contrast, the measurement of abnormally high creatinine levels showed good accuracy because the inaccuracy value remained within the ±10% range. Both normal and abnormally high creatinine measurements using the Jaffe method demonstrated good precision, as the coefficient of variation values were lower than the maximum CV limit of 6%.
Stability of Lyophilized Homemade Control Serum After Reconstitution on SGOT and SGPT Levels Stored in Freezer at Temperature (-2° to -4°C) and -20°C for 8 Weeks Kadek Profit Hartani; Anik Handayati
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 1 (2023): Step up to strengthen the laboratory system and prepare for patients care
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i1.3278

Abstract

Improving health laboratory services is closely related to strengthening the quality of health laboratories. Serum control is part of the quality assurance of health laboratories. Lyophilized homemade serum is a type of homemade control serum. This research aims to determine the stability of lyophilized homemade control serum against Serum Glutamic Oxaloacetic Transaminase (SGOT) and Serum Glutamic Pyruvic Transaminase (SGPT) levels. This research used an experimental method with pooled sera as research material stored at -2°C to -4°C and -20°C for 8 weeks. Pooled sera were obtained from respondents who had no history of disease, were free from HIV, AIDS, and HBsAg. The research was conducted at the Reference Laboratory and Clinical Chemistry Laboratory Politeknik Kesehatan (Poltekkes) Kementerian Kesehatan, Surabaya. The results of the study were data analysis using linear regression test. The SGOT parameter at freezer temperature (-2 to -4°C) showed that the storage time had an effect of 90.97% with an error component of 9.03%. At freezer temperature (-20°C), the result of storage time has an effect of 78.71% with an error component of 21.29%. In the SGPT parameter with freezer temperature (-2 to -4°C), the result of storage time has an effect of 96.71% with an error component of 3.29%. At freezer temperature (-20°C), the result of storage time has an effect of 91.47% with an error component of 8.53%. The results of the SGOT and SGPT examinations did not exceed the limits of ±2SD and ±3SD The CV on the SGOT and SGPT examinations also did not exceed the CCV limits, which is to be stable.
The Relationship Between TSH and Indirect Bilirubin Levels in Neonates Suspected of Having Jaundice Nur Alizah; Anik Handayati; Museyaroh Museyaroh; Suhariyadi Suhariyadi
Health Dynamics Vol 1, No 10 (2024): October 2024
Publisher : Knowledge Dynamics

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33846/hd11006

Abstract

Background: Congenital hypothyroidism (CH) is a condition of thyroid hormone deficiency that occurs at birth. The TSH (thyroid-stimulating hormone) test is crucial for diagnosing hypothyroidism. CH is known to cause prolonged unconjugated hyperbilirubinemia. Therefore, this study aimed to analyze the relationship between TSH and indirect bilirubin levels in neonates suspected of having jaundice. Methods: This is a non-experimental, retrospective study conducted at Lombok Dua Dua Lontar Mother and Child Hospital in Surabaya. The study involved data collection on neonates aged 2–7 days suspected of jaundice, whose TSH and indirect bilirubin levels were measured between November 2022 to April 2024. Results: Among 100 neonates, 62% were aged 2-4 days, while 38% were aged 5-7 days. The majority were male (56%), with female comprising 44%. Of the 100 neonates, only 1 (1%) had borderline TSH levels, while 99% had normal TSH levels. Hyperbilirubinemia was observed in 94% of the neonates, while 6% had normal indirect bilirubin levels. Statistical analysis using the Spearman correlation showed no significant link between TSH and indirect bilirubin levels (p = 0.802). Conclusions: While this study did not find a clear connection between TSH and indirect bilirubin levels in neonates suspected of having jaundice, one case of borderline TSH was identified. This neonate required referral to pediatric endocrinology, as untreated congenital hypothyroidism can lead to mental retardation. Despite limited research linking TSH and bilirubin levels in jaundiced neonates, routine screening for congenital hypothyroidism using TSH testing should be reconsidered. Future studies could benefit from focusing on specific causes of neonatal jaundice to help narrow down research questions in this area.
PENGARUH WAKTU PEMERIKSAAN GLUKOSA DARAH MENGGUNAKAN TABUNG PLAIN DAN TABUNG SERUM SEPARATOR Qonita Ardiyanti Maysarah; Anik Handayati; Museyaroh Museyaroh; Evy Diah Woelansari
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14080

Abstract

Blood glucose testing is one of the most important laboratory examinations for the diagnosis, monitoring, and evaluation of diabetes mellitus management. The accuracy of test results is influenced by various factors, particularly those occurring during the pre-analytical phase, including the type of blood collection tube used and the delay between sample collection and analysis. Delayed examination may reduce blood glucose concentration due to ongoing glycolysis within blood cells, thereby affecting the validity of laboratory results. This study aimed to analyze the effect of delayed examination time on blood glucose levels using plain tubes and Serum Separator Tubes (SST). An experimental study with a Two-Way Repeated Measures design was conducted. Serum samples obtained from seven respondents were collected into plain tubes and SST tubes and analyzed at 0, 5, and 10 hours using a Biobase Automatic Chemistry Analyzer. Data were analyzed using the Shapiro–Wilk normality test and Two-Way Repeated Measures ANOVA. The results showed that the mean blood glucose level in plain tubes decreased from 75.57 mg/dL at 0 hours to 64.29 mg/dL at 5 hours and 65.43 mg/dL at 10 hours. In contrast, blood glucose levels in SST tubes remained relatively stable at 75.71 mg/dL, 76.86 mg/dL, and 78.14 mg/dL at 0, 5, and 10 hours, respectively. The data were normally distributed (p > 0.05). Two-Way Repeated Measures ANOVA demonstrated that examination time, tube type, and their interaction had a statistically significant effect on blood glucose levels (p < 0.05). These findings indicate that delayed examination affects blood glucose levels in plain tubes, whereas SST tubes are able to maintain blood glucose stability for up to 10 hours after sample collection. ABSTRAK Pemeriksaan kadar glukosa darah merupakan salah satu pemeriksaan laboratorium yang berperan penting dalam menegakkan diagnosis, memantau perkembangan penyakit, serta mengevaluasi keberhasilan terapi pada pasien diabetes mellitus. Keakuratan hasil pemeriksaan dipengaruhi oleh berbagai faktor, khususnya pada tahap pra-analitik, seperti jenis tabung yang digunakan dan lamanya waktu antara pengambilan sampel dengan proses pemeriksaan. Penundaan pemeriksaan dapat menyebabkan penurunan kadar glukosa akibat proses glikolisis yang masih berlangsung di dalam sel darah sehingga memengaruhi validitas hasil pemeriksaan. Penelitian ini bertujuan untuk menganalisis pengaruh penundaan waktu pemeriksaan terhadap kadar glukosa darah menggunakan tabung plain dan Serum Separator Tube (SST). Penelitian menggunakan desain eksperimen dengan pendekatan Two Way Repeated Measure. Sampel berupa serum dari tujuh responden yang dimasukkan ke dalam tabung plain dan SST, kemudian dianalisis pada waktu 0 jam, 5 jam, dan 10 jam menggunakan Biobase Automatic Chemistry Analyzer. Analisis data dilakukan menggunakan uji normalitas Shapiro-Wilk dan uji Two Way Repeated Measure ANOVA. Hasil penelitian menunjukkan bahwa rerata kadar glukosa pada tabung plain mengalami penurunan dari 75,57 mg/dL pada 0 jam menjadi 64,29 mg/dL pada 5 jam dan 65,43 mg/dL pada 10 jam. Sebaliknya, kadar glukosa pada tabung SST tetap relatif stabil, yaitu 75,71 mg/dL, 76,86 mg/dL, dan 78,14 mg/dL pada masing-masing waktu pengamatan. Data penelitian berdistribusi normal (p>0,05). Hasil analisis menunjukkan bahwa waktu pemeriksaan, jenis tabung, serta interaksi keduanya memberikan pengaruh yang bermakna terhadap kadar glukosa darah (p<0,05). Dengan demikian, penundaan pemeriksaan menyebabkan perubahan kadar glukosa pada tabung plain, sedangkan penggunaan tabung SST mampu mempertahankan stabilitas kadar glukosa darah hingga 10 jam setelah pengambilan sampel.
PENGARUH WAKTU PEMISAHAN PLASMA TERHADAP KADAR GLUKOSA DARAH MENGGUNAKAN TABUNG NAF DAN TABUNG EDTA Hanun Zhalma; Anik Handayati; Sri Sulami Endah Astuti; Lully Hanni Endarini
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14120

Abstract

The accuracy of blood glucose results is influenced by various factors, one of the most important of which is the pre-analytical stage. Blood glucose levels can decrease if plasma separation is delayed, because blood cells continue to carry out the glycolysis process. The method used in this study was an experiment with a posttest-only control group design, employing purposive sampling that involved 5 respondents and 4 treatment conditions for each type of tube based on Federer’s formula. The sample consisted of sixth-semester students in the D3 medical laboratory technology programme who met the inclusion and exclusion criteria. Blood glucose levels were measured using the GOD-PAP method with a BIOBASE chemistry analyser. The results of the analysis showed that blood glucose levels in both types of tubes decreased as the plasma separation delay time increased. For NaF plasma, the One-Way ANOVA test yielded a p-value > 0.05, indicating that variations in the plasma separation delay time had no significant effect. In contrast, the Kruskal-Wallis test for EDTA plasma yielded a p-value < 0.05, which indicated a significant difference in each delay time variation. Based on these results, NaF plasma was better able to maintain stable blood glucose levels during the plasma separation delay compared to EDTA plasma. This is caused by the sodium fluoride content in the NaF tube, which acts as an antiglycolytic agent, so it is able to inhibit the glycolysis process and maintain stable glucose levels in the blood. ABSTRAK Keakuratan hasil glukosa darah dipengaruhi oleh berbagai faktor, salah satunya yang paling penting pada tahap pra-analitik. Kadar glukosa darah dapat mengalami penurunan apabila pemisahan plasma ditunda, karena sel-sel darah tetap melakukan proses glikolisis. Metode yang digunakan dalam penelitian ini adalah eksperimen dengan desain Posttest Only Control Group Design menggunakan teknik purposive sampling yang melibatkan 5 responden dan 4 perlakuan setiap jenis tabung berdasarkan rumus Federer. Pemilihan sampel terhadap mahasiswa Program Studi D3 Teknologi Laboratorium Medis semester 6 sesuai kriteria inklusi dan eksklusi. Pemeriksaan kadar glukosa darah dilakukan menggunakan metode GOD-PAP dengan BIOBASE Chemistry Analyzer. Hasil analisis menunjukkan bahwa kadar glukosa darah pada kedua jenis tabung mengalami penurunan seiring dengan bertambahnya waktu penundaan pemisahan plasma. Pada plasma NaF hasil uji One Way ANOVA diperoleh nilai p>0.05, sehingga variasi waktu penundaan pemisahan plasma tidak memberikan pengaruh yang signifikan. Sebaliknya, plasma EDTA uji statistik Kruskal Wallis menunjukkan nilai p<0.05, yang mengindikasikan adanya perbedaan yang signifikan pada setiap variasi waktu penundaan. Berdasarkan hasil tersebut, plasma NaF lebih mampu mempertahankan stabilitas kadar glukosa darah selama penundaan pemisahan plasma dibandingkan plasma EDTA. Hal ini disebabkan oleh kandungan natrium fluorida pada tabung NaF yang berperan sebagai agen antiglikolitik, sehingga mampu menghambat proses glikolisis dan menjaga kestabilan kadar glukosa dalam darah.
AKURASI KADAR LDL METODE INDIRECT TERHADAP METODE DIRECT Marina Putri Rahajeng; Anik Handayati; Musholli Himmatun Nabilah; Edy Haryanto
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14483

Abstract

Lipid profile examination is used to help assess the risk of cardiovascular disease. LDL level examination can be performed using the indirect method (Friedewald Formula) and the direct method (homogeneous test). The indirect method is more economical and is still frequently used in laboratories, but its accuracy is starting to be questioned at high triglyceride levels. This study aims to determine the accuracy of LDL levels using the indirect method (Friedewald Formula) compared to the direct method (homogeneous test) at triglyceride levels of 200-300 mg/dL. This study used a comparative analytical quantitative design with a paired cross-sectional approach. The study sample used primary data, namely the serum of patients with triglyceride levels of 200-300 mg/dL examined using the indirect and direct methods on the same sample. Data analysis was performed using the Wilcoxon test, mean %Bias, Bland-Altman, and linear regression. The results showed that there was no significant difference between the indirect and direct methods with a significance value (p > 0.05). The average %Bias value of -0.3962% still meets the acceptance limits of the National Cholesterol Education Program (NCEP). Bland-Altman analysis showed that most of the data were within the limits of agreement. Based on the results of the study, the indirect method (Friedewald Formula) has good accuracy and relatively comparable results with the direct method (homogeneous testing) at triglyceride levels of 200-300 mg/dL. ABSTRAK Pemeriksaan profil lipid digunakan untuk membantu menilai risiko penyakit kardiovaskular. Pemeriksaan kadar LDL dapat dilakukan menggunakan metode indirect (Friedewald Formula) dan metode direct (homogeneous assay). Metode indirect lebih ekonomis dan masih sering digunakan di laboratorium, tetapi akurasinya mulai diragukan pada kadar trigliserida tinggi. Penelitian ini bertujuan untuk mengetahui akurasi kadar LDL metode indirect (Friedewald Formula) terhadap metode direct (homogeneous assay) pada kadar trigliserida 200–300 mg/dL. Penelitian ini menggunakan desain kuantitatif analitik komparatif dengan pendekatan cross-sectional berpasangan. Sampel penelitian menggunakan data primer, yaitu serum pasien dengan kadar trigliserida 200–300 mg/dL yang diperiksa menggunakan metode indirect dan metode direct pada sampel yang sama. Analisis data dilakukan menggunakan uji Wilcoxon, mean %Bias, dan Bland-Altman. Hasil penelitian menunjukkan tidak terdapat perbedaan bermakna antara metode indirect dan metode direct dengan nilai signifikansi (p > 0,05). Nilai mean %Bias sebesar -0,3962% masih memenuhi batas keberterimaan National Cholesterol Education Program (NCEP). Analisis Bland-Altman menunjukkan sebagian besar data berada dalam batas kesepakatan. Berdasarkan hasil penelitian, metode indirect (Friedewald Formula) memiliki akurasi yang baik dan hasil yang relatif sebanding dengan metode direct (homogeneous assay) pada kadar trigliserida 200-300 mg/dL.
PERBEDAAN KADAR ELEKTROLIT MENGGUNAKAN BAHAN UJI SERUM DENGAN BAHAN UJI PLASMA HEPARIN Linatur Rosyidah; Edy Haryanto; Anik Handayati; Gesang Jukadiarko
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14622

Abstract

Electrolyte examination is performed to assess the levels of the major electrolytes in the blood. This examination is used to help establish the diagnosis of fluid balance disorders and acid-base imbalances in the body. The test materials commonly used for electrolyte examination are serum and heparinized plasma. Differences in the type of test material may affect the results of electrolyte examinations. This study aimed to determine the differences in electrolyte levels, including sodium, potassium, chloride, and ionized calcium, between serum and heparinized plasma test materials. This study employed a quantitative approach with an analytical observational design and a cross-sectional design. A total of 15 samples were obtained from patients undergoing electrolyte examinations at Muhammadiyah Babat General Hospital in February 2026. Electrolyte examinations were performed using the Erba Electrolyte Analyzer with the direct Ion-Selective Electrode (ISE) method. The results showed that the mean sodium levels were 139.29 mmol/L in serum and 136.49 mmol/L in heparinized plasma, the mean potassium levels were 3.79 mmol/L in serum and 3.58 mmol/L in heparinized plasma, the mean chloride levels were 106.77 mmol/L in serum and 105.63 mmol/L in heparinized plasma, and the mean ionized calcium levels were 1.00 mmol/L in serum and 0.89 mmol/L in heparinized plasma. The examination results were analyzed using the paired t-test and the Wilcoxon signed-rank test according to the data distribution. The results showed significant differences in sodium, potassium, and ionized calcium levels between serum and heparinized plasma test materials, with significance values of p < 0.05. Meanwhile, chloride levels showed no significant difference between serum and heparinized plasma test materials, with a significance value of p > 0.05. Based on the data analysis that had been conducted, significant differences were found in sodium, potassium, and ionized calcium levels between serum and heparinized plasma test materials, whereas chloride levels showed no significant difference. ABSTRAK Pemeriksaan elektrolit merupakan pemeriksaan untuk menilai kadar elektrolit utama di dalam darah. Pemeriksaan ini digunakan untuk membantu menegakkan diagnosis adanya gangguan keseimbangan cairan maupun ketidakseimbangan asam-basa dalam tubuh. Bahan uji yang umum digunakan pada pemeriksaan elektrolit adalah serum dan plasma heparin. Perbedaan jenis bahan uji dapat memengaruhi hasil pemeriksaan elektrolit. Penelitian ini dilakukan untuk mengetahui adanya perbedaan kadar elektrrolit, meliputi kadar natrium, kalium, klorida, dan kalsium terionisasi antara bahan uji serum dengan bahan uji plasma heparin. Penelitian ini menggunakan pendekatan kuantitatif observasional analitik dengan desain cross-sectional. Jumlah sampel sebanyak 15 sampel dari pasien yang menjalani pemeriksaan elektrolit di RSU Muhammadiyah Babat pada bulan Februari 2026. Pemeriksaan elektrolit dilakukan menggunakan alat Erba Electrolyte Analyzer dengan metode Ion Selective Electrode (ISE) secara langsung. Hasil pemeriksaan menunjukkan bahwa rerata kadar natrium pada serum sebesar 139,29 mmol/L dan plasma heparin sebesar 136,49 mmol/L, kadar kalium pada serum sebesar 3,79 mmol/L dan plasma heparin sebesar 3,58 mmol/L, kadar klorida pada serum sebesar 106,77 mmol/L dan plasma heparin sebesar 105,63 mmol/L, serta kadar kalsium terionisasi pada serum sebesar 1,00 mmol/L dan plasma heparin sebesar 0,89 mmol/L. Data hasil dari pemeriksaan elektrolit akan dianalisa menggunakan uji T-dependen dan uji Wilcoxon sesuai dengan distribusi data. Hasil uji pada kadar natrium, kalium, dan kalsium terionisasi antara bahan uji serum dengan bahan uji plasma heparin menunjukkan adanya perbedaan bermakna, dengan nilai signifikansi (p < 0,05). Sedangkan, pada kadar klorida tidak terdapat perbedaan bermakna antara bahan uji serum dan bahan uji plasma heparin dengan nilai signifikansi (p > 0,05). Berdasarkan analisis data yang telah dilakukan, ditemukan perbedaan yang bermakna pada kadar natrium, kalium, dan kalsium terionisasi antara bahan uji serum dengan plasma heparin, sedangkan kadar klorida tidak menunjukkan perbedaan bermakna.
HBsAg Screening Using Immunochromatography Method in Preoperative Patients at Dr. Soegiri Hospital, Lamongan Yuninda Zebina Salsabillah; Suhariyadi; Museyaroh; Anik Handayati
Jurnal Sehat Indonesia (JUSINDO) Vol. 8 No. 2 (2026): Jurnal Sehat Indonesia (JUSINDO)
Publisher : CV. Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59141/-.v8i2.559

Abstract

Patients undergoing surgery require HBsAg screening to determine their hepatitis B infection status. Hepatitis B remains a global health concern with a relatively high incidence rate, including in Indonesia. This study aimed to describe the results of HBsAg screening using the immunochromatographic method among preoperative patients at Dr. Soegiri Lamongan Hospital. This study employed a descriptive design with a cross-sectional approach. The research sample consisted of 100 preoperative patients selected using purposive sampling. HBsAg detection was performed using an immunochromatographic rapid test method. Data were analyzed using frequency distribution. The results showed that most preoperative patients had negative HBsAg results, with 92 samples (92%), while positive results were identified in 8 samples (8%), consisting of 4 male and 4 female patients aged 46–60 years. Based on these findings, it was concluded that the majority of preoperative patients tested negative for HBsAg, while positive HBsAg results were detected in a small proportion of samples.
Co-Authors ., Pestariati Alifa Christin Amalia Putri Khurota Ayyun Amania, Salsabila Anggie Banowati Wibiseno Anita Dwi Anggraini Arifin, Syamsul Atsania Putri , Puji Lestari Ayu Puspitasari Ayu Puspitasari, Ayu Caesaria, Adela Candra Triana Hikma Devan Andhika Dewa Ayu Puja Satya Dinarta Diah Titik Mutiarawati Edy Haryanto Edy Haryanto Edy Haryanto Edy Haryanto Edy Haryanto Ellies Tunjung Sari Maulidiyanti Endah Astuti, Sri Sulami Endarini, Lully Hanni Evy Diah Woelansari Fa'iqotul Azharil Jauharoh Fadia Rakhmalia Farah Adelia Qotrunnada Fitria Yulfirda Arini Fridayanti, Ni Made Risma Gesang Jukadiarko Hanun Zhalma Hapipah Hapipah Hikma , Candra Triana Ilma Ainun Nisa Istanto, Wisnu Jihan Azzahra Arsyi Juliana Christyaningsih Juliawan Apurwatama Kadek Profit Hartani Kartika Rahayuningsih, Christ Liliek Soetjiatie Linatur Rosyidah Luthfi Rusyadi Mamik Mamik Marina Putri Rahajeng Masdiva Putri Hidayah Maulida Azmi Wimasni Inah Maulida, Nailis Fathna Minarti Minarti Mohd Nazil Salleh Monica Putji Astuti Museyaroh Museyaroh Museyaroh, Museyaroh Musholli Himmatun Nabilah Nada Nabilah Wulandari Ni Wayan Lisna Marheni Ningsih, Agita Fortuna Septa Nur Alina Hidayatur Rizki Nur Alizah Nur Vita Purwaningsih, Nur Vita Patcharanee Pavadhgul Puji Lestari Atsania Putri Putri, Tiara Dewanti Qonita Ardiyanti Maysarah Rahmania, Adelia Prisma Retno Sasongkowati Rinza Rahmawati Samsudin Sri Sulami Endah Astuti Sri Utami Suhariyadi Suliati Suliati Syamsul Arifin Syamsul Arifin Tjipto Rini Winarni Winarni Yuni Eka Cahyana Yuninda Zebina Salsabillah