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All Journal Berkala Ilmu Kesehatan Kulit dan Kelamin Syntax Literate: Jurnal Ilmiah Indonesia Open Access Indonesian Journal of Medical Reviews Cermin Dunia Kedokteran Journal of Social Research Jurnal Locus Penelitian dan Pengabdian MEDICINUS Media Dermato-Venereologica Indonesiana Journal of General Procedural Dermatology and Venereology Indonesia Plexus Medical Journal
Kusumawardani, Arie
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Aerospace Engineering Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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Topical Fermented Lactobacillus acidophilus Lysate Accelerates Second-Degree Burn Healing: An In Vivo Study in Wistar Rats Kamilah, Lian; Kusumawardani, Arie; Widhiati, Suci; Dharmawan, Nugrohoaji; Ellistasari, Endra Yustin
Open Access Indonesian Journal of Medical Reviews Vol. 5 No. 4 (2025): Open Access Indonesian Journal of Medical Reviews
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37275/oaijmr.v5i4.725

Abstract

Second-degree burns, affecting the epidermis and dermis, constitute a major category of thermal injuries globally, presenting significant clinical challenges including pain, infection risk, and potential scarring. While standard treatments like silver sulfadiazine (SSD) exist, limitations including potential cytotoxicity and emerging resistance necessitate exploration of novel therapeutic strategies. Recent interest has focused on topical applications derived from probiotics, such as Lactobacillus spp., due to their suggested roles in modulating inflammation, combating oxidative stress, and providing antimicrobial activity to accelerate wound repair. This study investigated a fermented lysate derived from Lactobacillus acidophilus (L. fermented) as a potential topical agent for burn healing. This study aimed to evaluate the therapeutic efficacy of a 5% topical L. fermented ointment on the healing process of experimentally induced second-degree burns in a Wistar rat model, primarily by assessing the rate of wound closure compared to standard SSD treatment and an untreated control. A true experimental in vivo study utilizing a post-test only control group design was performed following ethical approval. Fifteen male Wistar rats were subjected to a standardized second-degree thermal burn injury on their dorsal aspect. The animals were then randomized (n=5 per group) to receive twice-daily topical applications of either 5% L. fermented ointment (Group A), SSD ointment (Group B), or no treatment (Control, Group C). Wound healing was quantitatively assessed by measuring the wound surface area (mm²) on days 1, 3, 7, and 14 post-injury using digital imaging and ImageJ software analysis. Statistical comparisons between groups were conducted using one-way ANOVA followed by LSD post-hoc tests, with statistical significance set at p<0.05. All treatment groups demonstrated a significant reduction in wound size over the 14-day observation period (p=0.001 within each group). Inter-group comparisons revealed significantly accelerated wound closure in Group A starting from day 3 onwards (p<0.005). At day 14, the mean wound area in Group A (17.5 ± 8.06 mm²) was significantly smaller than in Group B (119.22 ± 45.41 mm²) and Group C (305.18 ± 25.21 mm²) (p=0.001). Post-hoc analysis confirmed the superiority of L. fermented treatment over both SSD (mean difference 101.72 mm², p=0.001) and control (mean difference 287.68 mm², p=0.001). SSD treatment also resulted in significantly better healing than the control group (mean difference 185.96 mm², p=0.001). In conclusion, topical application of 5% fermented Lactobacillus acidophilus lysate significantly accelerated the closure of second-degree burn wounds in Wistar rats, demonstrating superior efficacy compared to both silver sulfadiazine treatment and no treatment. These findings highlight the potential of L. fermented lysate as a promising novel therapeutic agent for burn wound management.
Sikap dan Pemahaman Mahasiswa Kedokteran Semester Akhir Terhadap Laki Seks Lelaki sebagai Risiko Infeksi Menular Seksual dan HIV Hidayatullah, Lalu Muh Syahrul Rio; Kusumawardani, Arie; Ellistasari, Endra Yustin
Plexus Medical Journal Vol. 4 No. 4 (2025): Agustus
Publisher : Fakultas Kedokteran, Universitas Sebelas Maret

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/plexus.v4i4.2285

Abstract

Latar Belakang: Penelitian ini bertujuan untuk mengeksplorasi sikap dan Tingkat pemahaman mahasiswa Kedokteran semester akhir (semester tujuh) mengenai Infeksi Menular Seksual (IMS) dan risiko Human Immunodeficiency Virus (HIV) di kalangan Lelaki Seks Lelaki (LSL). Meningkatnya prevalensi IMS dan HIV di kalangan LSL menunjukkan perlunya perhatian yang lebih besar terhadap pendidikan kesehatan dan kewaspadaan terhadap risiko infeksi pada kelompok ini. Sebagai calon tenaga kesehatan, mahasiswa Kedokteran memiliki  peran penting dalam  mencegah  dan  mengatasi masalah kesehatan ini. Metode: Penelitian ini menggunakan metode observasional analitik dengan desain cross-sectional. Populasi dalam penelitian ini adalah mahasiswa Kedokteran semester akhir Universitas Sebelas Maret, dengan jumlah sampel sebanyak 233 responden ditentukan menggunakan teknik total sampling . Teknik analisis data menggunakan analisis deskriptif dan analisis data kuantitatif. Hasil: Hasil penelitian menunjukan sikap mahasiswa semester akhir Kedokteran terhadap LSL sebagai faktor risiko IMS dan infeksi HIV sebagian besar mempunyai sikap sedang (ragu – ragu) sebanyak 93,1% terhadap topik penelitian. Tingkat pemahaman mahasiswa Kedokteran semester akhir terhadap LSL sebagai faktor risiko IMS kategori baik hanya sebanyak 42,1% dan pada infeksi HIV yang mempunyai pemahaman baik hanya sebanyak 44,6%, hasil ini masih kurang baik dan perlu ditingkatkan sebagai mahasiswa Kedokteran. Terdapat adanya hubungan yang signifikan antara tingkat pemahaman dengan sikap mahasiswa Kedokteran semester akhir dengan nilai signifikansi (IMS (p value = 0,014) & HIV (p value = 0,026)). Simpulan: Mahasiswa Kedokteran semester akhir memiliki tingkat pemahaman yang baik, sikap kategori yang tinggi dan terdapat hubungan antara tingkat pemahaman dan sikap terkait topik LSL sebagai faktor risiko IMS dan HIV.
The Clinical Relevance of Serum Interleukin-21 in Grading Acne Vulgaris Dewi, Ayu Kusuma; Irawanto, Muhammad Eko; Kusumawardani, Arie; Dharmawan, Nugrohoaji; Mulianto, Nurrachmat
Journal of Social Research Vol. 4 No. 11 (2025): Journal of Social Research
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/josr.v4i10.2795

Abstract

Acne vulgaris (AV) is an inflammatory skin disorder with multifactorial pathogenesis involving genetics, hormones, and immune dysregulation. Interleukin-21 (IL-21), a pleiotropic cytokine mainly secreted by T follicular helper and Th17 cells, has been implicated in various inflammatory skin diseases. However, its role in AV remains unclear. This cross-sectional study included 46 patients with AV, classified into mild (n=19), moderate (n=16), and severe (n=11) groups. Serum IL-21 levels were measured using an enzyme-linked immunosorbent assay (ELISA). Baseline characteristics were compared across groups, and interobserver reliability of AV severity grading was evaluated using Cohen’s kappa test. One-way ANOVA was applied to assess differences in IL-21 levels among severity groups, with a significance level set at p<0.05. The mean serum IL-21 level was highest in the severe AV group (303.53 pg/mL), followed by the moderate (223.7 pg/mL) and mild (213.2 pg/mL) groups. Although there was a trend toward increasing IL-21 levels with higher AV severity, statistical analysis revealed no significant difference among the three groups (p=0.130). Serum IL-21 levels were elevated in AV patients compared with previously reported healthy controls but did not differ significantly across severity levels. These findings suggest that IL-21 may play a role in the pathophysiology of AV, though it is not a reliable marker of clinical severity. Further case-control and tissue-based studies are needed to clarify its role.
Pengaruh Derajat Keparahan Terhadap Kejadian Resistensi Doksisiklin pada Penderita Akne Vulgaris Widyastuti, Zilpa; Kariosentono, Harijono; Kusumawardani, Arie
Syntax Literate Jurnal Ilmiah Indonesia
Publisher : Syntax Corporation

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36418/syntax-literate.v9i3.14731

Abstract

Tujuan penelitian ini adalah untuk mengetahui pengaruh derajat keparahan akne vulgaris (AV) terhadap kejadian resistensi antibiotik doksisiklin. Penelitian ini merupakan penelitian observasional analitik dengan desain cross-sectional yang berlokasi di poliklinik kulit dan kelamin RSUD Dr. Moewardi, pada bulan Agustus hingga September 2023. Subjek pada penelitian ini penderita AV derajat sedang-berat. Derajat keparahan AV dinilai menggunakan sistem skoring Global Acne Grading System (GAGS), sedangkan kejadian resistensi doksisiklin dinilai dengan menggunakan metode disc diffusion. Terdapat sebanyak 31 subyek pada penelitian ini dengan AV derajat sedang-berat. Terdapat sebanyak 3 subjek (9,68%) yang mengalami resistensi doksisiklin. Hasil analisis data menunjukkan bahwa terdapat pengaruh yang signifikan dari derajat keparahan AV terhadap kejadian resistensi doksisiklin pada C. acnes (p = 0,041). Secara umum, dapat disimpulkan bahwa derajat keparahan AV berpengaruh terhadap timbulnya resistensi doksisiklin pada C. acnes.
PERBANDINGAN PEMBERIAN KRIM SERAMID DAN SHEA BUTTER PADA PENURUNAN TRANSEPIDERMAL WATER LOSS Novriana, Dita Eka; Kusumawardani, Arie
Media Dermato-Venereologica Indonesiana Vol 50 No 4 (2023): Media Dermato Venereologica Indonesiana
Publisher : Perhimpunan Dokter Spesialis Kulit dan Kelamin Indonesia (PERDOSKI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33820/mdvi.v50i4.457

Abstract

Pendahuluan: Kulit merupakan organ pelindung tubuh dari mikroorganisme dan bahan-bahan berba- haya lainnya. Kulit terdiri dari beberapa lapisan yaitu epidermis, demis, dan subkutan. Stratum korneum pada epidermis berperan untuk mencegah transepidermal waterloss (TEWL). Seramid dan shea butter merupakan bahan yang dapat berguna untuk membantu memperbaiki fungsi sawar kulit dengan mencegah TEWL. Tujuan: Untuk mengetahui efektivitas pemberian krim seramid dan shea butter terhadap penurunan TEWL. Metode: Penelitian ini dilakukan di Poliklinik Kulit dan Kelamin RSUD Dr. Moewardi pada Februari – Maret 2020 terdiri dari 15 laki-laki dan 15 perempuan berusia 20–30 tahun. Nilai TEWL diperiksa terlebih dahulu sebelum pemberian perlakuan. Krim seramid dioleskan pada sisi volar lengan bawah kanan dan shea butter dioles- kan pada sisi volar lengan bawah kiri, masing-masing sebanyak dua kali sehari selama empat minggu. Uji normalitas data menggunakan Saphiro Wilk dan uji statistik dengan Independent T-Test. Hasil: Penelitian ini menunjukkan adanya perubahan nilai TEWL yang signifikan secara statistik pada penggunaan krim seramid dibandingkan shea butter dengan nilai p=0.000 (p<0.05). Kesimpulan: Penurunan nilai TEWL pada krim seramid lebih bermakna secara statistik dibandingkan dengan shea butter.
Hubungan Derajat Keparahan dengan Status Psikososial Pasien Melasma : Studi Cross-sectional Sambodo, Shelly Lavenia; Ellistasari, Endra Yustin; Kusumawardani, Arie; Irawanto, Muhammad Eko; Mulianto, Nurrachmat
Jurnal Locus Penelitian dan Pengabdian Vol. 4 No. 12 (2025): JURNAL LOCUS: Penelitian dan Pengabdian
Publisher : Riviera Publishing

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58344/locus.v4i12.4993

Abstract

Melasma tidak hanya berdampak pada aspek fisik berupa hiperpigmentasi, tetapi juga menimbulkan gangguan psikososial yang signifikan. Hubungan antara tingkat keparahan klinis melasma dengan derajat gangguan psikososial belum banyak diteliti di populasi Indonesia. Menganalisis hubungan antara tingkat keparahan melasma menggunakan Melasma Area and Severity Index (MASI) dengan derajat status psikososial menggunakan Melasma Quality of Life (MELASQOL). Studi cross-sectional dilakukan pada 15 pasien wanita dengan melasma di RSUD Dr. Moewardi Surakarta. Tingkat keparahan dinilai menggunakan skor MASI, sedangkan status psikososial dinilai menggunakan kuesioner skor MELASQOL. Analisis korelasi Pearson dan regresi linear sederhana digunakan untuk mengevaluasi hubungan antar variabel.  Rerata skor MASI adalah 5,95±1,15 dengan 53,3% pasien memiliki tingkat keparahan sedang dan 46,7% dengan tingkat derajat berat. Rerata skor MELASQOL adalah 40,33±6,22 dengan distribusi yang sama. Terdapat korelasi positif sangat kuat antara skor MASI dan MELASQOL (r=0,988; p<0,001; R²=0,977). Persamaan regresi menunjukkan MELASQOL = 8,62 + 5,33 × MASI. Terdapat hubungan positif yang sangat kuat antara tingkat keparahan melasma dengan derajat gangguan psikososial.
Evaluating the Serofast State in Syphilis: A Study of Clinical Characteristics, Immunological Markers, and HIV Status Lidjaja, Lifesia Natali; Ellistasari, Endra Yustin; Irawanto, Muhammad Eko; Kusumawardani, Arie; Mulianto, Nurrachmat
Journal of Social Research Vol. 5 No. 4 (2026): Journal of Social Research
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/josr.v5i4.3097

Abstract

Syphilis remains a significant global health burden, particularly among high-risk populations such as men who have sex with men (MSM). A major challenge in management is the serofast state, where patients fail to achieve serological cure despite adequate treatment. The immunological mechanisms driving this, especially in the context of HIV co-infection, remain unclear. This study aims to evaluate clinical and immunological factors associated with serofast status versus serological cure. A retrospective cohort study was conducted on 40 patients diagnosed with syphilis (early latent, late latent, or secondary). Data on demographics, HIV status, CD4/CD8 counts, and pre- and post-treatment VDRL titers were analyzed. Bivariate and multivariate logistic regression analyses were performed to identify predictors of the serofast outcome. The cohort was predominantly males (90.0%) and unmarried (80.0%). HIV co-infection was identified as the sole independent predictor of the serofast state (aOR = 10.94; 95% CI: 1.72-69.65; p=0.011). While post-treatment VDRL titers significantly decreased in the total cohort, the serofast group retained significantly higher antibody levels compared to the serological cure group (p<0.001). However, no statistically significant differences were found in CD4 counts, CD8 counts, or CD4/CD8 ratios between the two groups. HIV co-infection is a strong predictor of the serofast state in syphilis patients. The lack of significant differences in quantitative cellular immune markers suggests that the persistence of antibodies may be driven by functional immune dysregulation rather than absolute T-cell depletion. These findings emphasize the need for vigilant serological monitoring in HIV-syphilis co-infected individuals.
The Effect of Oral Bromelain on Interleukin-6 Levels in Tissue of Acne Vulgaris Model Rats noviani, Laura; Irawanto, Muhammad Eko; Ellistasari, Endra Yustin; Kusumawardani, Arie; Mulianto, Nurrachmat
Journal of Social Research Vol. 5 No. 4 (2026): Journal of Social Research
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/josr.v5i4.3102

Abstract

Acne vulgaris is a chronic inflammatory disorder of the pilosebaceous unit associated with Cutibacterium acnes colonization and activation of the Toll-like receptor–NF-?B pathway, which upregulates production of inflammatory mediators such as interleukin-6 (IL-6). Bromelain, a proteolytic enzyme derived from pineapple (Ananas comosus), is known to possess anti-inflammatory properties; however, experimental evidence regarding its effect on inflammatory cytokines in acne remains limited. This study aimed to evaluate the effect of oral bromelain administration on IL-6 levels in the tissue of acne vulgaris model rats. This in vivo laboratory experimental study employed a posttest-only control group design. Fifteen male Sprague-Dawley rats induced with C. acnes were divided into three groups: normal control (K1), negative control (K2), and treatment group (P) receiving oral bromelain at 88 mg/kgBW/day for 21 days. Tissue IL-6 levels were measured by enzyme-linked immunosorbent assay (ELISA). Data were analyzed using one-way ANOVA followed by a post hoc least significant difference (LSD) test. C. acnes induction significantly elevated tissue IL-6 levels compared to the normal control (p<0.001). One-way ANOVA revealed statistically significant differences in IL-6 levels between groups (p<0.001). The lowest IL-6 levels were observed in K1 (36.50±0.43) and the highest in K2 (94.01±0.57). The treatment group (P; 47.46±0.83) demonstrated significantly lower IL-6 levels than K2 and approximated K1 values. The post hoc LSD test confirmed statistically significant differences across all pairwise group comparisons (p<0.001). Oral bromelain administration effectively reduced IL-6 levels in the tissue of acne vulgaris model rats, demonstrating significant anti-inflammatory activity. These findings suggest that bromelain has potential as an adjunct therapy for inflammatory control in acne vulgaris.
Correlation Between Serum Il-24 Level in Acne Vulgaris Patients of Varying Clinical Severity Evanti, Annisa Marsha; Irawanto, Muhammad Eko; Mulianto, Nurrachmat; Kusumawardani, Arie; Ellistasari, Endra Yustin
Journal of Social Research Vol. 5 No. 4 (2026): Journal of Social Research
Publisher : International Journal Labs

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55324/josr.v5i4.3104

Abstract

Background: Acne vulgaris (AV) is a chronic inflammatory disorder of the pilosebaceous unit that significantly impacts patients' quality of life. The inflammatory process involves immune responses partially regulated by IL-24, suggesting its potential role in AV pathophysiology and clinical severity. However, data on serum IL-24 levels based on AV severity remain limited, particularly in Indonesia. This study aims to evaluate the relationship between serum IL-24 levels and acne vulgaris severity to further elucidate its molecular mechanisms. Methods: This observational analytical study with a cross-sectional design was conducted at Dr. Moewardi General Hospital, Surakarta, Indonesia, from November 2025 to January 2026, involving healthy individuals and acne vulgaris patients. AV severity was assessed using the Lehmann classification, and serum IL-24 levels were measured by ELISA. Results: A total of 60 subjects were involved, consisting of 15 healthy controls, 15 patients with mild AV, 15 with moderate AV, and 15 with severe AV. Pearson correlation analysis showed no significant relationship between serum IL-24 levels and AV severity (p = 0.186). However, there was a trend towards increasing serum IL-24 levels along with acne severity. In the healthy control group, IL-24 levels were lower than in the AV group. Conclusions: Serum IL-24 levels are not associated with acne vulgaris severity. This is likely due to its effect on local keratinocyte proliferation. Further research is needed to elucidate the pathophysiology using histopathological examination of skin tissue from patients with AV
Co-Authors Anggraeni, Reti Annisa Marsha Evanti Ayu Kusuma Dewi Dharmawan, Nugrohoaji Dita Eka Novriana Ellistasari, Endra Yustin Febriani, Dina Hapsari, Jasmine Rahmi Hartati, Agung Triana Hidayatullah, Lalu Muh Syahrul Rio Indah Julianto, Indah Irawanto, Muhammad Eko Jalasena Mysea, Marsyanda Kamilah, Lian Kariosentono, Harijono Lidjaja, Lifesia Natali Mochtar, Moerbono Mulianto, Nurachmat Murasmita, Alamanda Mustifah, Etty Farida Nareswari, Adniana Noviani, Laura Nurrachmat Mulianto Prasetyorini, Budi Eko Prihadi, Ihsany Arafiasetyanto Rafika Paramitasari, Anggana Rahma, Alfina Rosita, Fiska Rosmarwati, Ervina Shelly Lavenia Sambodo Triasari Oktavriana Widhiati, Suci Widyastuti, Zilpa Yudhistira, Muhammad Yurizar Yustin Ellista Sari, Endra