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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology Buletin Peternakan Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture BERITA BIOLOGI Jurnal Penelitian Pertanian Terapan Livestock and Animal Research Animal Production : Indonesian Journal of Animal Production Tropical Animal Science Journal
Kustantinah Kustantinah
Department Of Animal Nutrition And Feed Science, Faculty Of Animal Science, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Energy Environmental Science Veterinary Other

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Physicochemical Characteristics, in Vitro Fermentation Indicators, Gas Production Kinetics, and Degradability of Solid Herbal Waste as Alternative Feed Source for Ruminants A. N. Kisworo; A. Agus; Kustantinah Kustantinah; B. Suwignyo
Media Peternakan Vol. 40 No. 2 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (515.23 KB) | DOI: 10.5398/medpet.2017.40.2.101

Abstract

The aims of this research were to study the nutrient and secondary metabolite contents of solid herbal wastes (SHW) that were preserved by freeze drying, sun drying and silage, as well as to analyze their effects on in vitro fermentation indicators i.e., gas production kinetics and degradability of solid herbal waste. Physical and chemical properties on three forms of SHW (sun dry, freeze dry, and silage) were characterized and then an in vitro gas production experiment was performed to determine the kinetics of gas production, methane production, NH3, microbial protein, and SHW degradability. Polyethylene glycol (PEG) was added to the three treatments to determine the biological activity of tannins. Results showed that all three preparations of SHW still contained high nutrient and plant secondary metabolite contents. Gas production, methane, NH3, microbial protein, in vitro degradability of dry matter (IVDMD) and organic matter (IVDOM) of SHW silage were lower (P<0.05) compared to sun dry and freeze dry. These results were apparently due to the high content of secondary metabolites especially tannin. It can be concluded that solid herbal wastes (SHW) can be used as an alternative feed ingredients for ruminants with attention to the content of secondary metabolites that can affect the process of fermentation and digestibility in the rumen.
Genetic Analysis Using Partial Sequencing of Melanocortin 4 Receptor (MC4R) Gene in Bligon Goat Latifah Latifah; D. A. Priyadi; D. Maharani; Kustantinah Kustantinah; T. Hartatik
Media Peternakan Vol. 40 No. 2 (2017): Media Peternakan
Publisher : Faculty of Animal Science, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4895.739 KB) | DOI: 10.5398/medpet.2017.40.2.71

Abstract

Melanocortin 4 Receptor gene is involved in sympathetic nerve activity, adrenal and thyroid functions, and media for leptin in regulating energy balance and homeostasis. The aim of this research was to perform genetic analysis of MC4R gene sequences from Bligon goats. Fourty blood samples of Bligon does were used for DNA extraction. The primers were designed after alignment of 12 DNA sequences of MC4R gene from goat, sheep, and cattle. The primers were constructed on the Capra hircus MC4R gene sequence from GenBank (accession No. NM_001285591). Two DNA polymorphisms of MC4R were revealed in exon region (g.998 A/G and g.1079 C/T). The SNP g.998 A/G was a non-synonymous polymorphism i.e., changing of amino acid from methionine (Met) to isoleucine (Ile). The SNP g.1079 C/T was a synonymous polymorphism. Restriction enzyme mapping on Bligon goat MC4R gene revealed three restriction enzymes (RsaI (GT’AC), Acc651 (G’GTAC_C), and KpnI (G_GTAC’C), which can recognize the SNP at g.1079 C/T. The restriction enzymes may be used for genotyping of the gene target using PCR-RFLP method in the future research.
Effect of Types and Dosages of Foliar Fertilizers on Morphology and Production of Clitoria ternatea Apriyani Nur Sariffudin; Nafiatul Umami; Bambang Suhartanto; Bambang Suwignyo; Kustantinah Kustantinah
Jurnal Ilmu Ternak dan Veteriner Vol 26, No 4 (2021): December 2021
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v26i4.2912

Abstract

The aim of this study was to determine effect of types and dose levels of foliar fertilizers on  morphology and production of Clitoria ternatea. This study was conducted from September to November 2020 at Forage Research Garden for Animal Feed and Pasture, Faculty of Animal Science, Gadjah Mada University, Yogyakarta. Materials used were Clitoria ternatea, liquid organic fertilizer of rabbit and gandasil D fertilizer. This study was designed in a Completely Randomized Design (CRD) with factorial pattern of 2x4. The fertilizer dosages were: 0,1.5,3.0 and 4.5 g/l/plot. The parameters measured were plant height, stem diameter, leaf area, number of branches, fresh and dry matter productions. Data were analyzed by the analysis of variance at 5%, with Duncan's Multiple Range Test (DMRT). Results showed that types of foliar fertilizers did not affect morphological characteristics and production of Clitoria ternatea (P>0.05), but the fertilizer dosages had significant effect (P<0.05) on morphological characteristics except for leaf area and production of Clitoria ternatea. The higher the dose level of foliar fertilizer the greater the plant height, stem diameter and number of branches. Dosage of 4.5 g/l/plot resulted in the highest plant height, stem diameter and number of branches each 160.02, 1.14 cm and 18.96 branches, respectively. Dosage of4.5 g/l/plot gave the highest fresh and dry matter production of 19.22 and 16.75 tons/ha. It is concluded that increasing the dosage level of foliar fertilizer up to 4.5 g/l/plot resulted in an increased quantity measures of several morphological characteristics and production.
Perbaikan Pakan Kambing Bligon Menggunakan Daun Ketela sebagai Suplemen (Feed Improvement of Bligon Goats Diet Using Cassava Leaf as Supplement) Kustantinah (Kustantinah); Arif Nur Wibowo; Hari Hartadi
Buletin Peternakan Vol 33, No 3 (2009): Buletin Peternakan Vol. 33 (3) Oktober 2009
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v33i3.111

Abstract

Agricultural by-product, especially from cassava plants, can be found easily at all over Indonesia. Parts of cassava (Manihot esculenta Crantz) plant that can be used for animal feed are leaves, stem covering, tuber (in dry orwet condition, with or without skin). Cassava leaves cannot be used freely as animal feed, because of the persistence of anti-quality, i.e. Cyanide Acid (HCN) and tannin substance. Cassava leaves can be found easily in the dry season, whengenerally the presence of forage as ruminant’s diet is so limited. This research had to be done to observe potency of cassava leaves as supplement for Bligon goat’s diet. This research used 24 mature early pregnant Bligon goats. Thegoat divided into 3 treatments, those are : K as Control Diet (conventional diet which usually be given by the farmers); T1 or Treatment 1 (Control Diet+300 g cassava leaves); and T2 or Treatment 2 (Control Diet+260 g cassavaleaves+200 dried cassava tuber). The result showed that cassava leaves supplementation increased EE and TDN consumption. From total consumption, cassava leaves and dried cassava tuber increased DM, OM, CP, EE, and TDNdigestibility, but reduced CF digestibility. The effect of anti-coccidia didn’t appear optimally which shown by there was no significant differences of the amount of coccidian oocyste in the goat feces. However, the amount of coccidianoocyste in the feces reduced (1666.67 on the T1 and 2500 on the T2) as compare to those on the K (5000).(Key words: Bligon Goat, Cassava leaves, Feed Supplement, Coccidiostat)
PENGARUH PENGGUNAAN DRIED DISTILLERS GRAIN WITH SOLUBLE (DDGS) PADA RANSUM BERENERGI RENDAH TERHADAP KARKAS, LEMAK ABDOMINAL, DAN HATI AYAM BROILER Edwin Indarto; Jamhari (Jamhari); Fatimah Zahra; Zuprizal (Zuprizal); Kustantinah (Kustantinah)
Buletin Peternakan Vol 35, No 2 (2011): Buletin Peternakan Vol. 35 (2) Juni 2011
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v35i2.593

Abstract

The experiment was conducted to find out the effect of Dried Distillers Grain with Soluble (DDGS) with low energy diet on carcass, abdomen fat and liver of broiler throughout 35 days growing period. This experiment was conducted using 125 had of day old chick. The birds were randomly devided into five dietary treatments. Each dietary treatment was assigned to five replicate pens containing five chicks. The diets contained 0, 20, 30, 40 and 50% DDGS. At 35-days old, 25 birds were weighed and slaughtered, then the carcass, abdomen fat and liver were weighed. The data were analyzed by Oneway Anova. The Duncan`s New Multiple Range Test (DMRT) were used to analyze the differences between means. There were significant effects of DDGS level on carcass weight and percentage, abdomen fat and percentage, and liver weight. Carcass weight of broiler chicken of R 0, R 20, R 30, R 40 and R 50 were 625.40±55.38, 608.60±55.61, 568.20±123.87, 422.80±55.97, and 401.80±47.53 g, carcass percentage were 61.79±1.83, 61.70±2.78, 60.17±2.57, 57.19±2.22, and 56.68±3.61%, abdomen fat weight were 15.60±3.28, 15.00±4.00, 13.20±5.63, 5.60±3.28, and 4.20±2.86 g, abdomen fat percentage were 1.56±0.42, 1.53±0.46, 1.37±0.46, 0.77±0.47, and 0.61±0.42%, and liver weight were 22.20±2.94, 21.80±3.96, 20.80±0.89, 15.80±2.77, and 15.80±1.92 g, respectively. It is concluded that the use of DDGS up to 30% could maintain the carcass, but the use of 40% DDGS decreased carcass weight and percentage, as well as the abdomen fat and liver.(Key words: Dried Distillers Grain with Soluble, Broiler chicken, Carcass, Abdomen fat, Liver)
KECERNAAN IN SACCO HIJAUAN LEGUMINOSA DAN HIJAUAN NON- LEGUMINOSA DALAM RUMEN SAPI PERANAKAN ONGOLE Rendi Fathoni Hadi; Kustantinah (Kustantinah); Hari Hartadi
Buletin Peternakan Vol 35, No 2 (2011): Buletin Peternakan Vol. 35 (2) Juni 2011
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v35i2.594

Abstract

This study was aimed to explore the optimum potential of forage and the degradation rate the rumen of nutrient of forage (legume and non legume) derived different forage fodder used farmers. Seven forages i.e. Gliricidia (GL),Manilkara (SW), Sesbania (TP), Ficus (BR), Calothyrsus (KL), Jackfruit (NG), and Cacao leaves (CO), were used in this experiment. Samples were incubated in the rumen of fistulated cattle with different time intervals, namely 2, 4, 8,12, 16, 24, 36, and 48 hours. The collected data were the nutrient digestibility residues in rumen, including dried matter (DM), organic matter (OM), crude protein (CP), neutral detergent fiber (NDF), and acid detergent fiber (ADF). Datawere analyzed using Completely Randomized Design (CRD). From the calculation of in sacco rumen, it was obtained the degradation theory (DT) values of DM : GL 60.06%; SW 34.78%; TP 73.96%; BR 41.40%; KL 43.80%; NG 43.67%; and CO 28.67%. From in sacco rumen calculation, it was obtained the DT values of OM : GL 59.13%; SW 32.74%; TP 73.07%; BR 42.83%; KL 44.18%; NG 45.04%; and CO 26.80%. Calculation of in sacco rumen showed the DT values of CP : GL 66.14%; SW 32.49%; TP 81.46%; BR 42.89%; KL 31.84%; NG 47.05%; and CO 29.46%. The calculation of in sacco rumen, it was obtained the DT values of NDF: GL 46.84%; SW 46.59%; TP 56.69%; BR 44.18%; KL 23.01%; NG 30.07%; and CO 55.43%. From the calculation of in sacco rumen, it was obtained the DTvalues of ADF: GL 61.27%; SW 43.95%; TP 56.53%; BR 40.11%; KL 21.08%; NG 44.66%; and CO 69.15%. There were significant differences (P<0.05) on the degradation of DM, OM, CP, NDF, and ADF. It is concluded that not all of legume has higher DT values of DM, OM, CP, NDF, and ADF fraction than non-legume. There is a tendency that the longer retention time in the rumen, the higher degradation rate.(Keywords: Legume, Non-legume, In sacco rumen)
The Effect of Protein Feed Supplementation to Various Roughage Basal Feed on The Performance of Bligon Goats Kustantinah (Kustantinah); H. Hartadi; L. M. Yusiati; R. Utomo; A. Agus; B. Suhartanto; F. Holil; E. Dahono
Buletin Peternakan Vol 29, No 4 (2005): Buletin Peternakan Vol. 29 (4) November 2005
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v29i4.1184

Abstract

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Distribusi Pendapatan Peternak Penggaduh Kambing Bligon di Gombang, Ponjong, Kabupaten Gunungkidul Nimas Resmita Kawuriaji; Sudi Nurtini; Kustantinah (Kustantinah)
Buletin Peternakan Vol 30, No 2 (2006): Buletin Peternakan Vol. 30 (2) Mei 2006
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v30i2.1200

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Efek Pengeringan dengan Waktu yang Berbeda terhadap Kecernaan In Vitro Gliricidia maculata Kustantinah (Kustantinah); H. Hartadi; B. Suhartanto; Soemitro Padmowijoto; Ristanto Utomo; Lies Mira Yusiati; Ali Agus
Buletin Peternakan Vol 31, No 4 (2007): Buletin Peternakan Vol. 31 (4) November 2007
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v31i4.1234

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Penggunaan Daun Ketela Pohon di Dalam Ransum untuk Mengatasi Endo Parasit pada Kambing yang Dipelihara Peternak Kustantinah (Kustantinah); Y. D. Artikarini; H. Hartadi; W. Nurcahyo
Buletin Peternakan Vol 32, No 1 (2008): Buletin Peternakan Vol. 32 (1) Februari 2008
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v32i1.1239

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Co-Authors A. A. Sakti A. Agus A. Agus A. N. Kisworo Agung Purnomoadi Agustinus Paga Ali Agus Ali Agus Apriyani Nur Sariffudin Arif Nindyo Kisworo Arif Nur Wibowo B. Suhartanto B. Suwignyo Bambang Suhartanto Bambang Suwignyo Budi Prasetyo Widyobroto Cuk Tri Noviandi D. A. Priyadi D. Maharani Diahanvika Tri Sarvinda Djoko Soetrisno E. Dahono E. Indarto E.R. Orskov Edwin Indarto Endang Baliarti F. Holil H. Hartadi Hari Hartadi Hery Widijanto I G S. Budisatria, I G I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria I Gede Suparta Budisatria Ismaya Ismaya Jamhari (Jamhari) Joko Daryatmo K Adhianto, K Kusuma Adhianto Kusuma Adhianto L. M. Yusiati Latifah Latifah Latifah, L. Lies Mira Yusiati Luis Tavares M Socheh Maharani, D. Muhlisin Muhlisin N Ngadiyono, N N. D. Dono Nafiatul Umami Nimas Resmita Kawuriaji Nono Ngadiyono Novie Shintasari Petrus Malo Bulu, Petrus Malo R. Utomo R. W. Nurcahyo Ratri Ratna Dewi Rendi Fathoni Hadi Retno Adiwiniarti, Retno Ristanto Utomo Rusman Rusman Setyono, W. Sigit Bintara Siti Muyasaroh Soemitro Padmowijoto Sudi Nurtini T. Hartatik T. Hartatik W. Nurcahyo Widya Kenshiana Putri Y. D. Artikarini Yusri Sapsuha Zulfa, I. H. Zuprizal (Zuprizal)