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All Journal Jurnal Penelitian Kesehatan Suara Forikes Health Notions THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Manuju : Malahayati Nursing Journal 2-TRIK: TUNAS-TUNAS RISET KESEHATAN Medica Arteriana (Med-Art) CENDEKIA : Jurnal Ilmu Pengetahuan International Journal of Advanced Health Science and Technology HEALTHY: Jurnal Inovasi Riset Ilmu Kesehatan Jurnal Pengabdian Kepada Masyarakat Mulawarman Jurnal Sehat Indonesia (JUSINDO) Analis Kesehatan Sains (e-Journal) Jurnal Laboratorium Khatulistiwa Frontiers in Community Service and Empowerment Vitamin: Jurnal Ilmu Kesehatan Umum Jurnal Media Analis Kesehatan Folia Medica Indonesiana
Anggraini, Anita Dwi
Poltekkes Kemenkes Surabaya
Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Electrical & Electronics Engineering Engineering Health Professions Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary Other

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Lymphocyte Count and SARS-CoV-2 Antibody Level in Healthy Donors' Blood at an Indonesian Blood Transfusion Center Prasasti, Adelia Gita; Woelansari, Evy Diah; , Suhariyadi; Anggraini, Anita Dwi
Folia Medica Indonesiana Vol. 59, No. 4
Publisher : Folia Medica Indonesiana

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Abstract

Highlights: 1. It is essential to conduct research on SARS-CoV-2 for the purpose of acquiring further understanding, especially concerning the production of antibodies examined using antibody titer blood tests. 2. Although the relationship between the examined variables is not significant, this study offers valuable information on blood test results after the COVID-19 vaccination, which can serve as scientific evidence for further research. Abstract Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a virus that infects the respiratory system by attacking the mucous and epithelial cells. This infection commonly leads to an increase in lymphocyte count as an immune response to invading pathogens. Moreover, antibodies bind and inactivate foreign substances to destroy pathogens and inhibit their replication. These mechanisms prompt the objective of this study, which was to define the relationship between lymphocyte count and SARS-CoV-2 antibody level. This analytical observational study used a cross-sectional approach with quantitative analysis methods and purposive sampling. Healthy donors who had received coronavirus disease (COVID-19) vaccines provided the samples for this study. A total of 30 blood samples were collected from the Blood Transfusion Center of the Indonesian Red Cross Surabaya Area. This study was conducted in May 2022 at two distinct locations. The examination of lymphocytes was carried out using the flow cytometry method in the Hematology Laboratory, Department of Medical Laboratory Technology, Politeknik Kesehatan Kemenkes Surabaya, Surabaya, Indonesia. In addition, the antibody titer test using the enzyme-linked immunosorbent assay (ELISA) method was performed in the Immunoserology Laboratory of the Surabaya Health Laboratory Center, Surabaya, Indonesia. The analysis revealed an average lymphocyte concentration of 2.2633 x 103/µl and an average antibody level of 0.2197 according to the optical density (OD) ratio. The data analysis was performed using Spearman's rank correlation statistical test (p<0.005), and the results indicated a lack of significance with p=0.262. In conclusion, there is no relationship between total lymphocyte count and SARS-CoV-2 antibody level.
Expression of Immunoglobulin G / Immunoglobulin M Typhoid On Neutrophil-Lymphocyte Ratio In Patients At Haji Hospital, Surabaya Nur Zerlinda; Evy Diah Woelansari; Anita Dwi Anggraini
International Journal of Advanced Health Science and Technology Vol. 3 No. 4 (2023): August
Publisher : Forum Ilmiah Teknologi dan Ilmu Kesehatan (FORITIKES)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35882/ijahst.v3i4.289

Abstract

Typhoid fever is one of the major bacterial infections worldwide caused by the bacterium Salmonella enterica serovars typhi in humans. The examination used is the rapid Typhoid test. This rapid typhoid test is used to detect antibodies to Salmonella typhi. The purpose of the study was to determine the results of the Neutrophil - Lymphocyte Ratio with various criteria results from the Immunoglobulin G / Immunoglobulin M Test in typhoid fever patients. This type of study is descriptive observational with selective sampling taken. The samples in this study were 28 samples conducted in April 2023 on typhoid fever patients at the Hajj Hospital in East Java Province. This study used the Rapid Typhoid Test Immunochromatography method with positive Widal criteria and supporting examination using Neutrophil–Lymphocyte Ratio (NLR) with Hematology Analyzer tool Fluorescent Flow Cytometry method. Typhoid and NLR rapid test results are processed descriptively in tables and based on percentages. The results obtained from the rapid test were positive IgM of 29%, positive IgG of 11%, positive IgG and IgM of 3%, and negative IgG and IgM of 57%. The average NLR was 4,43% with a median of 2,85. IgG / IgM examination of NLR showed no significant association in typhoid fever patients. It is hoped that this research will be further developed using Salmonella culture examination.
EFEKTIVITAS EKSTRAK ETANOL KAYU SECANG (Caesalpinia sappan L.) TERHADAP WAKTU KEMATIAN CACING Ascaris suum SECARA IN VITRO Firashinta Yudi Ardityasari; Retno Sasongkowati; Anita Dwi Anggraini
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14111

Abstract

ABSTRACT Worm infections caused by ascariasis remain a public health problem in Indonesia due to their high prevalence and their impact on nutritional status and growth. The use of synthetic anthelmintics as the primary treatment has limitations in the form of side effects and the potential for resistance, making natural alternatives necessary. Sappan wood (Caesalpinia sappan L.) is known to contain flavonoids, saponins and tannins that have potential as anthelmintics. This was an experimental study with six treatment groups: a negative control (distilled water), a positive control (0.25% pyrantel pamoate), and secang wood ethanol extracts at concentrations of 4%, 8%, 12%, and 15%. The study was conducted from October 2025 –May 2026 at the Parasitology Laboratory of the Department of Medical Laboratory Technology, Poltekkes Kemenkes Surabaya. The ethanol extract of secang wood was prepared at the Faculty of Veterinary Medicine, Airlangga University, whilst the Ascaris suum worms were obtained from the Surabaya Slaughterhouse. The results of the study showed that all concentrations of the ethanol extract of secang wood were capable of causing the death of all worms by the 10th hour. In the positive control, all worms died by the 2nd hour, whilst in the negative control, no worm mortality was observed. Based on the results of the study, it can be concluded that ethanol extract of secang wood exhibits anthelmintic activity against Ascaris suum at all test concentrations. The 12% concentration was the lowest effective concentration capable of causing the death of 50% of the worm population (LC50) with a time to 50% mortality (LT50) of 6 hours, although its efficacy was still lower than that of 0.25% pyrantel pamoate. ABSTRAK Infeksi kecacingan akibat askariasis masih menjadi masalah kesehatan di Indonesia karena prevalensinya tinggi serta berdampak pada status gizi dan pertumbuhan. Penggunaan antelmintik sintetik sebagai terapi utama memiliki keterbatasan berupa efek samping dan potensi resistensi sehingga diperlukan alternatif berbasis bahan alam. Kayu secang (Caesalpinia sappan L.) diketahui mengandung flavonoid, saponin, dan tanin yang berpotensi sebagai antelmintik. Metode penelitian ini adalah eksperimental dengan enam kelompok perlakuan, yaitu kontrol negatif (aquadest), kontrol positif (pirantel pamoat 0,25%), serta ekstrak etanol kayu secang konsentrasi 4%, 8%, 12%, dan 15% yang dilaksanakan pada Oktober 2025–Mei 2026 di Laboratorium Parasitologi Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya. Ekstrak etanol kayu secang dibuat di Fakultas Kedokteran Hewan Universitas Airlangga, sedangkan cacing Ascaris suum diperoleh dari Rumah Pemotongan Hewan Surabaya. Hasil penelitian menunjukkan bahwa seluruh konsentrasi ekstrak etanol kayu secang mampu menyebabkan kematian seluruh cacing pada jam ke-10. Pada kontrol positif, seluruh cacing mati sejak jam ke-2, sedangkan pada kontrol negatif tidak ditemukan kematian cacing. Berdasarkan hasil penelitian dapat disimpulkan bahwa ekstrak etanol kayu secang memiliki aktivitas antelmintik terhadap Ascaris suum pada seluruh konsentrasi uji. Konsentrasi 12% merupakan konsentrasi efektif terendah yang mampu menyebabkan kematian 50% populasi cacing (LC50) dengan waktu kematian 50% populasi (LT50) selama 6 jam, meskipun efektivitasnya masih lebih rendah dibandingkan pirantel pamoat 0,25%.
UJI EFEKTIVITAS ANTIJAMUR EKSTRAK KAYU SECANG (CAESALPINIA SAPPAN L.) TERHADAP PERTUMBUHAN JAMUR TRICHOPHYTON RUBRUM Rosadatul Hikmah Ramadhani; Retno Sasongkowati; Anita Dwi Anggraini
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14386

Abstract

Dermatophyte infection is one of the most common skin health problems in tropical countries such as Indonesia, with Trichophyton rubrum being the primary causative agent of dermatophytosis affecting skin tissues. Continuous use of synthetic antifungal drugs may cause adverse effects and increase the risk of antifungal resistance, highlighting the need for natural antifungal alternatives. Sappan wood (Caesalpinia sappan L.) contains bioactive compounds, including flavonoids, brazilin, alkaloids, tannins, and terpenoids, which have potential antifungal properties. This study aimed to determine the effectiveness of sappan wood extract in inhibiting the growth of Trichophyton rubrum. This in vitro experimental study employed the disk diffusion method on Sabouraud Dextrose Agar (SDA), which was selected because it is a simple and widely used preliminary screening method for evaluating antifungal activity based on the formation of inhibition zones around the test disks. The study was conducted at the Parasitology Laboratory, Department of Medical Laboratory Technology, Health Polytechnic of the Ministry of Health Surabaya, in February 2026. Ethanolic extract of sappan wood was obtained through maceration using 96% ethanol and tested at concentrations of 20%, 40%, 60%, 80%, and 100%, with 2% ketoconazole as the positive control and sterile distilled water as the negative control, each performed in four replicates. The results showed that all concentrations of sappan wood extract failed to produce inhibition zones against Trichophyton rubrum, whereas the positive control produced a mean inhibition zone diameter of 20 mm and the negative control showed no inhibition zone. It can be concluded that sappan wood extract at concentrations of 20%–100% was not effective in inhibiting the growth of Trichophyton rubrum using the disk diffusion method. ABSTRAK Infeksi jamur dermatofita merupakan salah satu masalah kesehatan kulit yang banyak dijumpai di negara beriklim tropis seperti Indonesia, dengan Trichophyton rubrum sebagai penyebab dermatofitosis yang paling sering menyerang jaringan kulit. Penggunaan obat antijamur sintetik secara terus-menerus berisiko menimbulkan efek samping dan resistensi, sehingga diperlukan alternatif antijamur berbahan alami. Kayu secang (Caesalpinia sappan L.) diketahui mengandung senyawa aktif seperti flavonoid, brazilin, alkaloid, tanin, dan terpenoid yang berpotensi sebagai antijamur. Penelitian ini bertujuan mengetahui efektivitas ekstrak kayu secang dalam menghambat pertumbuhan jamur Trichophyton rubrum. Penelitian bersifat eksperimental in vitro menggunakan metode difusi cakram pada media Sabouraud Dextrose Agar (SDA), yang dipilih karena merupakan metode sederhana dan umum digunakan sebagai uji awal (screening) untuk mengevaluasi aktivitas antijamur berdasarkan pembentukan zona hambat di sekitar cakram uji. Penelitian dilaksanakan di Laboratorium Parasitologi Jurusan Teknologi Laboratorium Medis Poltekkes Kemenkes Surabaya pada Februari 2026. Ekstrak etanol kayu secang diperoleh melalui metode maserasi menggunakan pelarut etanol 96% dan diuji pada konsentrasi 20%, 40%, 60%, 80%, dan 100%, dengan ketokonazol 2% sebagai kontrol positif dan akuades steril sebagai kontrol negatif, masing-masing diulang empat kali. Hasil penelitian menunjukkan bahwa seluruh konsentrasi ekstrak kayu secang tidak menghasilkan zona hambat terhadap pertumbuhan Trichophyton rubrum, sedangkan kontrol positif menghasilkan zona hambat dengan diameter rata-rata 20 mm dan kontrol negatif tidak menunjukkan zona hambat. Berdasarkan hasil tersebut, disimpulkan bahwa ekstrak kayu secang pada konsentrasi 20%–100% tidak efektif menghambat pertumbuhan jamur Trichophyton rubrum dengan metode difusi cakram.
PENGGUNAAN METHYLENE BLUE SEBAGAI PENGGANTI HEMATOKSILIN PADA SEDIAAN HISTOLOGI Naysilla Wardaniya; Juliana Christyaningsih; Lully Hanni Endarini; Anita Dwi Anggraini
HEALTHY : Jurnal Inovasi Riset Ilmu Kesehatan Vol. 5 No. 4 (2026)
Publisher : Pusat Pengembangan Pendidikan dan Penelitian Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51878/healthy.v5i4.14653

Abstract

Histological staining plays an essential role in enhancing the visualization of cellular and tissue structures. Hematoxylin, the nuclear stain used in the Hematoxylin–Eosin (HE) method, has several limitations, including relatively high cost and limited storage stability, necessitating the development of more economical alternatives such as methylene blue. This laboratory experimental study aimed to evaluate the use of methylene blue as a substitute for hematoxylin in mouse liver histological sections. The control group was stained using the Hematoxylin–Eosin method, while the treatment groups received methylene blue at concentrations of 10%, 11%, and 13%. Slide quality was evaluated microscopically using a scoring method based on nuclear clarity, cytoplasmic appearance, and color uniformity, followed by Kruskal–Wallis and Mann–Whitney analyses. The control group produced 100% good-quality slides. The 10% methylene blue group yielded 16.7% good and 83.3% fairly good slides. The 11% concentration produced 83.3% fairly good and 16.7% poor slides, whereas the 13% concentration resulted in 66.6% fairly good and 33.3% poor slides. The Kruskal–Wallis test revealed a significant difference among the groups (p=0.000; p<0.05). These findings indicate that methylene blue, particularly at a concentration of 10%, has potential as an alternative nuclear stain to hematoxylin because it produced the best staining quality among the treatment groups. ABSTRAK Pewarnaan sediaan histologi berperan penting dalam memperjelas struktur sel dan jaringan. Hematoksilin sebagai pewarna inti pada metode Hematoksilin-Eosin (HE) memiliki keterbatasan, antara lain harga yang relatif mahal dan stabilitas penyimpanan yang kurang baik, sehingga diperlukan alternatif pewarna yang lebih ekonomis, seperti methylene blue. Penelitian ini bertujuan mengetahui penggunaan methylene blue sebagai pengganti hematoksilin pada sediaan histologi jaringan hati mencit. Penelitian menggunakan desain eksperimental laboratorium dengan kelompok kontrol pewarnaan Hematoksilin-Eosin dan kelompok perlakuan methylene blue konsentrasi 10%, 11%, dan 13%. Kualitas preparat dinilai secara mikroskopis menggunakan metode skoring berdasarkan kejelasan inti sel, sitoplasma, dan keseragaman warna, kemudian dianalisis menggunakan uji Kruskal–Wallis dan Mann–Whitney. Hasil menunjukkan kelompok kontrol menghasilkan 100% preparat berkategori baik. Pada methylene blue 10% diperoleh 16,7% preparat berkategori baik dan 83,3% cukup baik, konsentrasi 11% menghasilkan 83,3% cukup baik dan 16,7% tidak baik, sedangkan konsentrasi 13% menghasilkan 66,6% cukup baik dan 33,3% tidak baik. Uji Kruskal–Wallis menunjukkan perbedaan yang signifikan antar kelompok (p=0,000; p<0,05). Dengan demikian, methylene blue berpotensi digunakan sebagai pewarna alternatif pengganti hematoksilin, terutama pada konsentrasi 10% yang menghasilkan kualitas preparat terbaik di antara kelompok perlakuan.
Co-Authors ., Pestariati Adelia Gita Prasasti Amelia, Noer Arin, Okvitasari Armelia Gitasari Nurhasanah Ayu Puspitasari Briansky, Sabira Alvayogi Deddy Adam Dellanis Arina Putri Diah Titik Mutiarawati Edy Haryanto Edy Haryanto Endah Astuti, Sri Sulami Endarini, Lully Hanni Era Fitria Yunita Ersanto, Novi Evy Diah Woelansari Febrianti Fitria Firashinta Yudi Ardityasari Fitria Yulfirda Arini Fitria, Febrianti Handayati, Anik Haqqi, Faishol Isnaini, Dian Istanto, Wisnu Juliana Christyaningsih Kartika Rahayuningsih, Christ Kristiningsih, Elly Kuswanto, Muhammad Lestari, Ari Fajar M. Zakarahman Makruf, Anang Maudita, Nauril Mitra Putri Mandiri Muhammad Sholakhuddin Al-Ayubi Museyaroh, Museyaroh Mutiarawati, Diah Titik Naysilla Wardaniya Niswata, Navila Arfianti Novita, Devy Nur Zerlinda Nurhasanah, Armelia Gitasari Okvitasari Arin Prasasti, Adelia Gita Puspitasari, Erlita Revi Zakiyatul Maftukhah Rosadatul Hikmah Ramadhani Rusmania, Liya Safrudin, Samsul Saktiyani, Fisari Sasongkowati, Retno Sugihani Suhariyadi Suhariyadi Suliati Suliati Syamsul Arifin Toni Watoyani Trisnania, Iid Dani Widyawati, Keri Ayu