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HUBUNGAN STATUS INSTABILITAS MIKROSATELIT DAN EKSPRESI P53 DENGAN ETIOPATOLOGI ADENOKARSINOMA KOLOREKTAL PADA ORANG INDONESIA DI KELOMPOK USIA KURANG DARI 40 TAHUN Kiki Lukman; Brigitta M. Dewayani; Bethy S. Hernowo; Basrul Hanafi; Tri Hanggono Achmad; Suwandi Sugandi
Indonesian Journal of Applied Sciences Vol 2, No 1 (2012)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8084.818 KB) | DOI: 10.24198/ijas.v2i1.2730

Abstract

Epidemiologically, the percentage of colorectal adenocarcinoma (CRC) in the age group ≤40 years old in Indonesia is higher than in the West European, North American and other developed Asian countries with different clinico-pathological features. In the afromentioned countries, the carcinogenesis pathway of this group is hereditary which show high microsatellite instability (MSI), whereas the age group >40 years old is sporadic of which show chromosomal instability (CIN) with the mutation of p53 gene. To elucidate the carcinogenesis pathway with its molecular characteristics of this group among Indonesians, a cross sectional study was conducted by performing the immunohistochemical detection of MLH1 and MSH2 expression (MSI status), and mutated p53 gene expression (CIN) in the CRC group ≤40 years old and >40 years old at Dr. Hasan Sadikin Hospital, Bandung, from May 2008 until November 2009. Thirty nine CRC patients, consisting of 22 patients of  ≤40 years old and 17patients >40 years old, were eligible for this study. The CRC patients of ≤40 years old showed 4 MSI high,  1 MSI lowand 17 MSS (microsatellite stable), associated with 10 p53 positive and 12 p53 negative status. In the CRC group of >40 years old, there was no MSI high, but 4 MSI low and 13 MSS were found, associated with 11 p53positive and 6 p53negative status. There was no significant difference with regards to the association between MSI status and p53 expression in both groups (p MSI=0.95, p p53=0.23).
REAKSI SILANG RESPONS IMUN HUMORAL VIRUS A/H1N1 PANDEMI/2009 DAN SEASONAL/2006 PADA REMAJA PERIODE KELAHIRAN TAHUN 1990-1996 MEITA DHAMAYANTI; TRI HANGGONO ACHMAD; CISSY B KARTASASMITA; PONPON IDJRADINATA
Indonesian Journal of Applied Sciences Vol 3, No 2 (2013)
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8874.663 KB) | DOI: 10.24198/.v3i2.16830

Abstract

Spinocerebellar Ataxia 3 (SCA3) Patient with Peripheral Neuropathy Siti Aminah Sobana; Iin Pusparini; Nushrotul Lailiyya; Ahmad Rizal Ganiem; Uni Gamayani; Yusuf Wibisono; Fathul Huda; Yunia Sribudiani; Tri Hanggono Achmad
Majalah Kedokteran Bandung Vol 54, No 1 (2022)
Publisher : Faculty of Medicine, Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15395/mkb.v54n1.2394

Abstract

Spinocerebellar ataxia (SCA) 3 is a neurodegenerative disease which involves cerebellum and extra cerebellum. Neuropathy in SCA3 manifests in various ways, including axonal and demyelination lesions in sensory and motor nerves. There has not been any study that describes the peripheral neuropathy characteristics of SCA3 patients in Indonesia at the time of this publication. This paper reports a case of a 43-year-old male with known spinocerebellar ataxia 3 presented with hereditary ataxia and mild numbness in both palms since two years before. No abnormalities were found during the sensory examination. The NCS showed severe axonal demyelinating sensorimotor peripheral neuropathy. In magnetic resonance imaging (MRI), an atrophy in the cerebellum with cerebral multiple lacunar infarction was identified. Electrophysiological results revealed profound axonal lesion in peripheral nerves. To conclude, peripheral neuropathy in SCA3 represents the dominance of axonal lesions in motor nerves.
GAMBARAN MOTIVASI MENJADI DOKTER PADA MAHASISWA TAHUN PERTAMA FAKULTAS KEDOKTERAN UNIVERSITAS PADJADJARAN sari puspa dewi; Insi Farisa Arya; Achadiyani -; Tri Hanggono Achmad
Jurnal Sistem Kesehatan Vol 1, No 1 (2015): Volume 1 Nomor 1 September 2015
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.133 KB) | DOI: 10.24198/jsk.v1i1.10338

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Dokter adalah profesi yang luhur dan dibutuhkan untuk memberikan pelayanan kesehatan kepada masyarakat. Pendidikan kedokteran merupakan pendidikan yang tidak mudah dan membutuhkan motivasi yang kuat untuk menyelesaikannya. Motivasi internal maupun eksternal telah diketahui dapat memengaruhi proses belajar maupun hasil belajar mahasiswa. Dengan mengetahui motivasi mahasiswa maka program studi dapat merancang kegiatan pembelajaran yang dapat meningkatkan motivasi mahasiswa. Penelitian ini bertujuan untuk mengetahui motivasi mahasiswa kedokteran memilih pendidikan dokter dan persepsinya terhadap profesi dokter. Penelitian ini merupakan penelitian kualitatif dengan metode fenomenologi. Seluruh mahasiswa yang masuk tahun 2014, pada bulan pertamanya ditugaskan menuliskan motivasi memilih program pendidikan dokter. Esai tersebut lalu dianalisis untuk mendapatkan kesamaan tema. Seluruh mahasiswa sebanyak 281 orang (209 perempuan, 72 laki-laki) menyatakan bahwa motivasi menjadi dokter terutama adalah untuk menolong dan menjaga kesehatan masyarakat. Motivasi lain adalah ingin mempelajari tubuh manusia lebih mendalam. Yang lain menyatakan faktor agama dan dorongan keluarga. Hampir semua mahasiswa memandang profesi kedokteran adalah profesi yang selalu dibutuhkan. Beberapa mahasiswa memandang profesi dokter masih menjanjikan kesejahteraan secara finansial. Data ini menunjukkan bahwa mahasiswa tahun pertama masih memiliki motivasi yang luhur untuk menjadi dokter. Hal ini akan dapat membantu mereka dalam menempuh pendidikan. Penelitian lanjut perlu dilakukan untuk mengetahui perubahan motivasi mereka setelah lulus dokter.Kata kunci: motivasi, mahasiswa, profesi dokter
Korelasi antara Kadar Testosteron dan Proses Remodeling Ventrikel Kiri pada Penderita Infark Miokardium Akut Mohammad Rizki Akbar; Tri Hanggono Achmad; Ieva B. Akbar; Budhi Setianto Purwowiyoto
Global Medical & Health Communication (GMHC) Vol 4, No 2 (2016)
Publisher : Universitas Islam Bandung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.998 KB) | DOI: 10.29313/gmhc.v4i2.2008

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Infark miokardium akut merupakan penyebab utama kematian di dunia. Perbedaan jenis kelamin berperan terhadap mortalitas jangka panjang pascainfark miokardium yang menunjukkan gambaran pola fisiologi regenerasi miokardium yang spesifik. Kematian setelah infark miokardium lebih tinggi pada perempuan. Remodeling ventrikel kiri merupakan proses penyembuhan luka pascainfark miokardium yang menjadi petunjuk keadaan gagal jantung maupun kematian. Proses ini berpengaruh penting pada fungsi ventrikel dan prognosis survival yang dapat didiagnosis dengan pemeriksaan ekokardiografi. Terdapat kontroversi berkaitan dengan peranan androgen pada proses remodeling jantung. Walaupun masih terdapat perdebatan, androgen memiliki peran terhadap remodeling ventrikel kiri dan bersifat protektif terhadap proses fibrosis yang maladaptif. Dilakukan penelitian observasional analitik yang bersifat prospektif untuk mengkaji peranan testosteron terhadap remodeling ventrikel kiri pada pasien infark miokardium akut di RSUP Dr. Hasan Sadikin Bandung selama Maret–Oktober 2015. Penelitian dilakukan pada 60 orang laki-laki usia 40–77 tahun penderita infark miokardium akut. Pemeriksaan ekokardiografi, pengukuran kadar testosteron total, testosteron bebas, dan testosteron bioavailabel dilakukan sebanyak dua kali. Pemeriksaan pertama dilakukan saat pasien didiagnosis infark miokardium akut dan pengulangan 4–6 minggu kemudian. Usia rata-rata penderita 56,16±8,48 tahun. Bila dibanding dengan pemeriksaan pertama dan kedua, tampak peningkatan kadar testosteron total yang signifikan (785,00±661,76 ng/dL vs 822,33±365,64 ng/dL; p=0,004), penurunan kadar testosteron bebas (24,66±17,91 ng/dL vs 19,00±15,24 ng/dL; p=0,067), dan penurunan kadar testosteron bioavailabel (475,21±353,10 ng/dL vs 394,98±314,85 ng/dL; p=0,166). Analisis korelasi rank Spearman memperlihatkan korelasi bermakna antara testosteron bebas dan relative wall thickness (p=0,019), serta testosteron bioavailabel dengan relative wall thickness (p=0,014). Simpulan, testosteron berperan pada proses remodeling ventrikel kiri pascainfark miokardium akut yang diperlihatkan dengan peningkatan kadar testosteron total serta penurunan kadar testosteron bebas maupun testosteron bioavailabel yang memiliki afinitas yang kuat dengan kardiomiosit. CORELLATION BETWEEN TESTOSTERONE LEVEL AND LEFT VENTRICULAR REMODELING PROCESS IN ACUTE MYOCARDIAL INFARCTION PATIENTMyocardial infarction (MCI) is a leading cause of death worldwide. Gender differences in long term mortality after MCI lead to a specific physiologic pattern of myocardial regeneration. Moreover mortality after MCI was reported to be higher in women. Left ventricular remodeling is cardiac wound healing after MCI indicate a high risk of heart failure and death. This remodeling can importantly affect the function of the ventricle and prognosis for survival which can be diagnosed by echocardiography. Controversial information excert about the role of androgen in cardiac remodeling. Even the evidence still debatable, androgen has a role in left ventricular (LV) remodeling and protect heart from maladaptive fibrosis. A prospective analytical observational study was conducted to evaluate the role of testosterone in LV remodeling in acute myocardial infarction patients. The study comprised 60 men aged 40–77 years with acute myocardial infarction in Dr. Hasan Sadikin Hospital during March–October 2015. Echocardiographyc study and the level of total, free, and bioavailable testosterone were measured twice. The first measured when they diagnosed acute myocardial infarction and the second after 4–6 weeks. The age of patient was 56.16±8.487 years old. Comparing the first and the second measure indicate that total testosterone significantly increased (785.00±661.76 ng/dL vs 822.33±365.64 ng/dL, p=0.004), free testosterone decreased (24.66±17.91 ng/dL vs 19.00±15.24 ng/dL, p=0.067), and bioavailable testosteron decreased (475.21±353.10 ng/dL vs 394.98±314.85 ng/dL, p=0.166). Correlation analysis by rank Spearman showed significantly correlation between free testosterone with relative wall thickness (p=0.019), and bioavailable testosterone with relative wall thickness (p=0.014). It is concluded that testosterone has a role on LV remodeling process after myocardial infarction showed by increasing of total testosterone and decreasing of free and bioavailable testosterone which have great affinity with cardiomyocyte.
Quantitative Measure to Differentiate Wicket Spike from Interictal Epileptiform Discharges Suryani Gunadharma; Ahmad Rizal; Rovina Ruslami; Tri Hanggono Achmad; See Siew Ju; Juni Wijayanti Puspita; Sapto Wahyu Indratno; Edy Soewono
Communication in Biomathematical Sciences Vol. 4 No. 1 (2021)
Publisher : Indonesian Bio-Mathematical Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/cbms.2021.4.1.2

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A number of benign EEG patterns are often misinterpreted as interictal epileptiform discharges (IEDs) because of their epileptiform appearances, one of them is wicket spike. Differentiating wicket spike from IEDs may help in preventing epilepsy misdiagnosis. The temporal location of IEDs and wicket spike were chosen from 143 EEG recordings. Amplitude, duration and angles were measured from the wave triangles and were used as the variables. In this study, linear discriminant analysis is used to create the formula to differentiate wicket spike from IEDs consisting spike and sharp waves. We obtained a formula with excellent accuracy. This study emphasizes the need for objective criteria to distinguish wicket spike from IEDs to avoid misreading of the EEG and misdiagnosis of epilepsy.
Natural resistance-associated macrophage protein 1 gene polymorphisms in thalassemia patients with tuberculosis infection Mohammad Ghozali; Sari Puspa Dewi; Reni Ghrahani; Ani Melani Maskoen; Lelani Reniarti; Edhyana Sahiratmadja; Tri Hanggono Achmad
Paediatrica Indonesiana Vol 56 No 2 (2016): March 2016
Publisher : Indonesian Pediatric Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (299.222 KB) | DOI: 10.14238/pi56.2.2016.84-9

Abstract

that needs regular blood transfusions leading to accumulation of iron in the cells. This iron overload level in macrophage might cause intracellular bacteria, particularly Mycobacterium tuberculosis (MTB) to multiply. Polymorphisms in natural resistance-associated macrophage protein 1 (NRAMP1), a metal transporter across the phagosome membrane, play important role in regulating iron, which is also needed by MTB. Increased iron in thalassemia patients may have an increased potential risk for TB.Objective To compare natural resistance-associated macrophage protein 1 (NRAMP1) gene polymorphisms (INT4, D543N, and 3’UTR) in thalassemia patients with and without tuberculosis (TB) infection.Methods A cross-sectional measurement of NRAMP1 genetic polymorphisms was performed in pediatric thalassemia patients with TB (n=40) and without TB (n=50). Iron status including serum iron, total iron-binding capacity (TIBC), and ferritin, was compared between the two groups. The NRAMP1 genetic polymorphisms were analysed using polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP). Allelic and genotypic distributions of each polymorphism were assessed for possible associations with TB infection.Results Mean serum iron and TIBC in thalassemia patients with TB were higher compared to thalassemia patients without TB (mean serum: 166.26 vs. 134.92 μmol/L, respectively; P=0.026) and (mean TIBC: 236.78 vs. 195.84 μmol/L, respectively; P=0.029). In thalassemia patients with TB, we observed significantly higher frequency of the C allele in INT4 (10% vs. 2%, respectively; OR=5.44; 95%CI 1.1 to 26.4; P=0.02) and the TGTG deletion allele (78.8% vs. 51%, respectively; OR=3.56; 95%CI 1.83 to 6.9; P=0.0002) in 3’UTR polymorphisms than in thalassemia patients without TB. There were no significant differences in distributions of the A allele between TB and non-TB groups (16.3% vs. 15%, respectively; P=0.84) or the GA genotype (32.5% vs. 30%, respectively; P=0.79) in D543N.Conclusion The NRAMP1 polymorphisms are known to be associated with major gene susceptibility to TB, and in our thalassemia patients this association is even more pronounced.
Hubungan Peringkat Akreditasi Institusi Pendidikan Diploma III Kebidanan dengan Hasil Uji Coba Uji Kompetensi Bidan Periode Juli 2013 Ani Kusumastuti; Tri Hanggono Achmad; Tina Dewi Judistiani; Dany Hilmanto; Anita Deborah Anwar; Sari Puspa Dewi
Jurnal Pendidikan dan Pelayanan Kebidanan Indonesia (Indonesian Journal of Education and Midwifery Care Vol 2, No 2 (2015): Juni
Publisher : Program Studi Magister Kebidanan FK UNPAD

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (90.844 KB) | DOI: 10.24198/ijemc.v2i2.41

Abstract

Penjaminan mutu institusi pendidikan dilakukan melalui akreditasi institusi, sedangkan kualitas lulusan salah satunya dilakukan melalui uji kompetensi. Peringkat akreditasi yang baik akan menghasilkan uji kompetensi yang baik, namun berdasarkan hasil uji coba uji kompetensi bidan tahun 2012 didapatkan rerata nilai peserta dari institusi terakreditasi B memiliki nilai yang lebih rendah dari institusi yang terakreditasi C. Tujuan penelitian ini adalah mengetahui perbedaan nilai berdasarkan peringkat akreditasi dan hubungan peringkat akreditasi dengan nilai uji coba uji kompetensi. Penelitian dilakukan dengan  analitik cross sectional menggunakan seluruh data peserta uji coba uji kompetensi bidan periode Juli 2013 dari Asosiasi Institusi Pendidikan Kebidanan Indonesia (AIPKIND) dan Badan Akreditasi Nasional Perguruan Tinggi (BAN-PT). Data dianalisis dengan Kruskal-Wallis, Post Hoc Mann Whitney, dan Somers’d. Perbedaan nilai didapatkan pada semua kelompok akreditasi institusi (p<0,001) dengan nilai median (minimal−maksimal) 61,67 (19,44−78,33) akreditasi A, 54,44 (1,11−77,78) akreditasi B, dan 49,44 (2,78−80,00) akreditasi C (p<0,001). Hubungan yang lemah antara peringkat akreditasi institusi pendidikan dengan nilai uji coba uji kompetensi (p<0,001), baik dibandingkan dengan nilai median (r=0,22) maupun nilai batas lulus (r=0,23). Penelitian ini menunjukkan adanya perbedaan nilai uji coba uji kompetensi berdasarkan peringkat akreditasi dan adanya hubungan antara peringkat akreditasi institusi dengan hasil uji coba uji kompetensi bidan. Semakin baik peringkat akreditasi institusi maka semakin baik pula hasil uji coba uji kompetensi yang didapatkan
Faktor Risiko Kejadian Sepsis Neonatorum Awitan Dini di Rumah Sakit Umum Pusat Dr. Hasan Sadikin Bandung Namira Oktaviani Suwarna; Tetty Yuniati; Adi Imam Cahyadi; Tri Hanggono Achmad; Dwi Agustian
Sari Pediatri Vol 24, No 2 (2022)
Publisher : Badan Penerbit Ikatan Dokter Anak Indonesia (BP-IDAI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14238/sp24.2.2022.99-105

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Latar belakang. Sepsis neonatorum merupakan salah satu penyakit infeksi pada bayi baru lahir yang masih menjadi masalah utama di negara berkembang seperti Indonesia. Faktor risiko terjadinya sepsis neonatorum awitan dini dapat dikelompokkan menjadi faktor ibu dan bayi. Faktor risiko ibu seperti ketuban pecah dini dan riwayat infeksi pada kehamilan, sedangkan faktor bayi adalah prematuritas, berat badan lahir rendah, skor APGAR rendah, air ketuban bercampur mekonium, asfiksia neonatorum, dan skor APGAR rendah.Tujuan. Penelitian ini bertujuan untuk mengetahui faktor risiko yang berhubungan dengan kejadian sepsis neonatorum awitan dini di RSUP Dr. Hasan Sadikin Bandung Metode. Penelitian ini merupakan studi observasional potong lintang pada neonatus yang lahir hidup di RSUP Dr. Hasan Sadikin Bandung periode Januari 2018 sampai Desember 2019 dengan metode total sampling. Analisis statistik menggunakan chi-square dan analisis multivariat dengan regresi logistik. Hasil bermakna apabila nilai p<0,05. Hasil. Penelitian ini menunjukkan bahwa angka kejadian sepsis neonatorum awitan dini tahun 2018 – 2019 adalah 8,1% dari 5224 subjek. Analisis bivariat chi square dan multivariat dengan regresi logistik menunjukkan faktor risiko seperti ketuban pecah dini OR 1,69 (p=<0,001; IK95%: 1,27 – 2,25), usia kehamilan 34 – 37 minggu OR 1,59 (p=0,036; IK95%:1,03 – 2,45), usia kehamilan <34 minggu OR 8,65 (p=<0,001; IK95%:5,47 – 13,70), sectio caesarea OR 1,42 (p=0,002;IK95%:1,14 – 1,77), dan berat badan lahir <2500 gram OR 2,59 (p=<0,001; IK95%: 1,73–3,89) mempunyai hubungan bermakna terhadap kejadian sepsis.Kesimpulan. Penelitian ini menunjukkan bahwa faktor risiko ibu dan bayi seperti ketuban pecah dini >18 jam, usia kehamilan <37 minggu, sectio caesarea dan berat badan lahir <2500 gram memiliki hubungan yang bermakna dengan kejadian sepsis neonatorum awitan dini.
CHARACTERISTICS OF PATIENTS WITH URINARY TRACT STONES AND PROFILE OF URIC ACID AND CHOLESTEROL AT KUSTATI ISLAMIC GENERAL HOSPITAL SURAKARTA IN THE PERIOD OF OCTOBER 2020 TO MARCH 2022 Muhamad, Prasojo; Utomo, Ahmad Bi; Achmad, Tri Hanggono
Indonesian Journal of Urology Vol 30 No 2 (2023)
Publisher : Indonesian Urological Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32421/juri.v30i2.873

Abstract

Objective: This study aims to research the characteristic of patients with urinary tract stones and the profiles of uric acid and cholesterol at the Kustati Islamic General Hospital (RSUI) in the period from October 2020 to March 2022. Material & Methods: This research uses the cross-sectional retrospective approach with the descriptive method. The research subjects are the patients with urinary tract stones that have undergone operations in Kustati Islamic General Hospital in the period between October 2020 and March 2022 as well as patients that have had their level of uric acid and cholesterol examined in the laboratory of Kustati Islamic General Hospital in the period from October 2020 to March 2022. Results: Urinary tract stones occur the most in men (70.3%) and in the age group 46-59 years old with a percentage nearing 50%. Urinary tract stones occur the most in the upper section of the urinary tract, mainly in the kidneys (67.2%). Hyperuricemia occur mostly in men while hypercholesterolemia occur mostly in women. Conclusion: The location of stone is mostly found in the kidneys. Patients with hyperuricemia are mostly men, while patients with hypercholesterolemia are mostly women.