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All Journal HAYATI Journal of Biosciences Biospecies Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Bioscience Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Jurnal AGROSAINS dan TEKNOLOGI Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis JURNAL KESEHATAN PERINTIS Jurnal Hilirisasi IPTEKS (JHI) Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Best Journal (Biology Education, Sains and Technology) BioEksakta : Jurnal Ilmiah Biologi Unsoed Jurnal Zona Bioscientist : Jurnal Ilmiah Biologi Berkala Penelitian Hayati
Anthoni Agustien
Universitas Andalas
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Earth & Planetary Sciences Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary Other

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Journal : HAYATI Journal of Biosciences

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Optimization of Protease Activity of Endophytic Bacteria EUA-136 and EUA-139 from Bruguiera gymnorrhiza Using Response Surface Methodology Agustien, Anthoni; Zovia, Miftahul; Dwitaviani, Rima; Marlida, Yetti
HAYATI Journal of Biosciences Vol. 32 No. 2 (2025): March 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.2.426-435

Abstract

Protease is a vital enzyme used in industries such as detergents, pharmaceuticals, and animal feed, with a growing demand in the enzyme market. Endophytic microorganisms can produce stable proteases with a rapid synthesis process. This study optimized conditions of temperature, pH, salinity, agitation, and nutrient sources for protease production by EUA-136 and EUA-139 bacterial isolates. The research used Response Surface Methodology (RSM) with a Central Composite Design (CCD) in Design Expert Software 13.1 to identify optimal conditions and the bacterial isolates. The optimum conditions for the EUA-136 bacterial isolate to produce protease were 3% inoculum at 30 ºC, pH 7, 28.5 ppt salinity, and 150 rpm agitation. For the EUA-139 bacterial isolate, the optimum conditions were a carbon source of 1% (v/v) maltose, a nitrogen source of 1% (v/v) KNO3, casein as the inducer, and an inoculum concentration of 7.5% (v/v). Molecular identification of isolates EUA-136 and EUA-139 revealed similarities to Bacillus cereus strain 3TC-3 and Bacillus paramycoides 3665, respectively.
Species Diversity of Genus Aspergillus, Endophytic Fungal Isolated From Mangrove Ceriops tagal and their Antibiotic Potential Sophia, Anggun; Agustien, Anthoni; Chairul; Syamsuardi
HAYATI Journal of Biosciences Vol. 32 No. 5 (2025): September 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.5.1174-1184

Abstract

The rapid increase in antimicrobial resistance has encouraged development of new natural and urgent strategies to fight drug-resistant pathogens, one of which is using endophytic fungi. Endophytic fungi found in Ceriops tagal mangrove plant, which can adapt to extreme salinity environments of up to 60 ppt. Endophytic fungi are isolated from leaves, branch, and roots. Each isolate was identified morphologically and cultured in potato dextrose broth media for 21 days. After incubation, liquid culture was extracted with ethyl acetate and evaporated to obtain a thick extract. The Kirby Bauer method was used to evaluate the antibiotic potential of each endophytic fungal extract against pathogenic microbes Staphylococcus aureus, Escherichia coli, and Candida albicans. Concentration used was 5%, antibiotics used as positive controls were chloramphenicol (30μg/disk), nystatin (100 IU/disk), as negative controls DMSO. Three endophytic fungi were isolated from roots (ECT7, ECT 8, ECT 10), two from leaves (ECT 26, ECT 28) and six from branches (ECT 31, ECT 47, ECT 48, ECT 51, ECT 55 and ECT 85). Fungal isolates ECT10 has been proven to effectively exhibit strong antibiotic activity against microorganisms E. coli, S. aureus, and C. albicans, with inhibition zones measuring 13.0 mm, 11.8 mm, and 11.6 mm, respectively.
Co-Authors - Jumawita . Mansyurdin Abrar, Mohammad Aghni Aznia Akmal Djamaan Alviche, Lola Anggun Sophia Armaleni Armaleni Ayu Putri Ningsih Chairul Chairul Chairul Cici Wulandari Desma Yuni Deswati Deswati Devi Syafriyani Dewi Yudiana Shinta Dianty Putri Dina Wahyuna Djong Hon Tjong Dwitaviani, Rima Efrizal Efrizal Efrizal Efrizal Fadhila Cantika Ferry Lismanto Syaiful Feskaharny Alamsjah Fuji Astuti Febria Herwita Idris Hesti Dwi Marcellinna Ikel Fitri Yanis Ilham, Kurniadi Ilsa Septia Putri Irdawati Irdawati Irfan Mustafa Jetty Nurhajati Kurniadi Ilham Kustiasih Lestari Linar Z. Udin Lola Alviche Maliza, Rita Marlina Marlina Melisa Ainil Fajri Mifthahul Jannah Mildawati Mildawati Mira Rosnawita Muhammad Diki Juliandi Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nurhajati, Jetty Nurmiati Nurmiati Nurmiati Nurmiati Nurmiati, N Periadnadi Periadnadi PINGKAN ADITIAWATI Pingkan Aditiawati Putri, Silvy Rizka Rafique, Mohsin Rahmadani Eka Pitri Rahmayati, Riesca Salsabilah Rahmi Adila Rahmi, Inayati Riesca Salsabilah Rahmayati Riski Budi Yani RR. Ella Evrita Hestiandari Rusnam Rusnam Suwirmen, Suwirmen Syafrita, Assyifa Syamsuardi Syamsuardi Syukria Ikhsan Zam Tesri Maideliza Tri Widia Ningsih Alam Triyatdipa, Haqil Uci Mela Sari Udin, Linar Z. Widia Firliani Wira Hastuti Wiwik Handayani Yetria Rilda Yetti Marlida Yurike Nova Edlin Zakaria, Indra Junadi Zovia, Miftahul Zozy Aneloi Noli Zuhri Syam Zuhri Syam