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All Journal METAMORFOSA Journal of Biological Sciences Jurnal Veteriner Jurnal Biologi Udayana Bumi Lestari Jurnal Pendidikan Biologi Undiksha Jurnal Ilmu Dasar BIOTROPIA - The Southeast Asian Journal of Tropical Biology Jurnal Biosains Pascasarjana JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Pendidikan dan Sosial Humaniora SIMBIOSIS
Anak Agung Ketut Darmadi
Program Studi Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Udayana
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Dentistry Earth & Planetary Sciences Education Energy Environmental Science Health Professions Immunology & microbiology Mathematics Veterinary

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Journal : SIMBIOSIS

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POTENSI FILTRAT JAMUR Trichoderma sp. DALAM MENGHAMBAT PERTUMBUHAN Aspergillus sp. PADA PAKAN JAGUNG (Zea mays L.) Ni Made Fiona Ranika; Ni Made Gari; Anak Agung Ketut Darmadi
SIMBIOSIS Simbiosis Volume 13 No. 1 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i01.

Abstract

Corn is a crop that is used as a strategic commodity for Indonesia as it can be used as animal feed. However, corn production is easily decreased due to the presence of Aspergillus sp. fungi which is able to produce aflatoxin that can affect the livestock health problems. Based on this issue, a method of controlling Aspergillus is needed by using Trichoderma sp. fungus filtrate which has antagonistic properties to pathogens. The purpose of this study was to determine the potential of Trichoderma sp. fungus in inhibiting the growth of Aspergillus sp. isolated from corn grain feed, to obtain the concentration of Trichoderma sp. filtrate that is optimal in suppressing the growth of Aspergillus sp. pathogens and to determine the comparison of positive control inhibitory power with Trichoderma sp. filtrate treatment on Aspergillus sp. pathogens. This study used a Complete Randomized Design (RAL) with dual culture methods and diffusion wells with different doses, namely 10% (v / v), 25% (v / v), 40% (v / v), 55% (v / v), and 70% (v / v). The results showed that the fungus and Trichoderma sp. filtrate had a significant effect in inhibiting the growth of Aspergillus sp. (P ≤ 0,05). The percentage of inhibition of Trichoderma sp. in inhibiting the growth of Aspergillus sp. with dual culture method was 68,61 ± 0,83%. Another study of Trichoderma sp. filtrate test on Aspergillus sp. produced the largest clear zone in the 70% treatment with a diameter of 14,81 ± 0,45 mm and the smallest was found in the 10% treatment with a diameter of 9,06 ± 0,55 mm, while in the positive control treatment a diameter of 19,53 ± 0,94 mm was obtained. The filtrate capability is expected to be a solution for related parties in improving the quality of corn grain feed.
ISOLASI DAN UJI PATOGENITAS JAMUR Colletotrichum spp. PENYEBAB PENYAKIT ANTRAKNOSA PADA BUAH TOMAT (Solanum lycopersicum L.) : ISOLATION AND PATHOGENITY TESTING OF FUNGI Colletotrichum spp. CAUSES OF ANTHRACNOSE DISEASE ON TOMATOES (Solanum lycopersicum L.) Laila Maghfirotun Ni’mah; Sudirga, Sang Ketut; Darmadi, Anak Agung Ketut
SIMBIOSIS Simbiosis Volume 13 No. 2 2025
Publisher : Program Studi Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JSIMBIOSIS.2025.v13.i02.p02

Abstract

Tomatoes (Solanum lycopersicum L.) is a fruit with important economic value and is a leading horticultural commodity in Indonesia. Anthracnose is a disease that can infect tomato plants. From previous research, several types of isolates of Colletotrichum spp. Infecting different hosts can cause different disease severity. The aim of this research was to identify the Colletotrichum fungus that causes anthracnose on tomatoes from several gardens in Bali to test its level of pathogenicity. Isolation of the fungus Colletotrichum spp. using the direct planting method. The pathogenicity test uses the disease severity (KP) scoring method using the formula. Identification and morphological characterization of isolates of Colletotrichum spp. using the identification book "Illustrated Genera of Imperfect Fungi". The parameters observed were colony shape, hyphae and conidia characters and disease severity percentage (KP) on tomatoes. This research used a completely randomized design (CRD) and the data were analyzed statistically using the ANOVA (Analysis of Variance) test with SPSS version 26 software. The results of the identification of macroscopic and microscopic characters from PS, BS and SW isolates showed differences in colony, hyphae and conidia characters. The pathogenicity test results of the PS, BS, and SW isolates showed that the SW isolate was the most adaptive and pathogenic isolate. Keywords: Identification, isolate, hyphae, spores, disease severity
Co-Authors . Ramdhoani Abd. Rahman As-syakur Ainun Sida Alprida Yanti Andy Agustina Lande Aulia Nurbaiti Dwi Ariani Yulihastuti ENIEK KRISWIYANTI Ferdinan, David Firman Hani, Ni Luh I Gusti Ayu Sugi Wahyuni I Ketut Endra Primantara I Ketut Ginantra I Komang Alit Adi Sanjaya I Putu Gde Ardhana I Wayan Suanda Ida Bagus Gede Darmayasa Ida Bagus Made Bramasta Wira bumi Ida Bagus Made Bramasta Wirabumi Inabuy, Fainmarinat selviani Inna Narayani Irawati Irawati IRIANI SETYAWATI Kadek Mardika Laila Maghfirotun Ni’mah Martin Joni NI KADEK LOSIANI Ni LUH ARPIWI Ni Luh Arpiwi Ni Made Fiona Ranika Ni Made Gari NI NYOMAN DARSINI Ni Nyoman Wirasiti Ni Wayan Sudatri Putri Permata Ratnasari, Athalia Retno Kawuri Rochmalia Juniarti Putri SANG KETUT SUDIRGA SANG KETUT SUDIRGA Shetty, Kalidas Silviana Febrionita Meiskia Agung Sista Nirmala SURYANINGSIH - Wijana, Made Sara Wijaya, I Made Saka Wira bumi, Ida Bagus Made Bramasta Yan Ramona