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All Journal Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia AGRIKA Plumula : Berkala Ilmiah Agroteknologi Jurnal Ilmiah Pertanian BIOEDUSCIENCE Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) International Journal of Community Service Learning G-Tech : Jurnal Teknologi Terapan PLAKAT : Jurnal Pelayanan Kepada Masyarakat Journal of Community Service and Empowerment Nusantara Science and Technology Proceedings Bioscientist : Jurnal Ilmiah Biologi Jurnal Pengabdian dan Edukasi Sekolah (Jubaedah) Jurnal Abdimas Bina Bangsa Batara Wisnu : Indonesian Journal of Community Services Journal of Applied Agricultural Science and Technology Journal of Community Service Jurnal HPT (Hama Penyakit Tumbuhan) RADIKULA: Jurnal Ilmu Pertanian Journal of Applied Plant Technology Jurnal Pengabdian Sosial
Ramadhani Mahendra Kusuma
Agrotechnology Study Program, Agriculture Faculty, Universitas Pembangunan Nasional“Veteran” Jawa Timur, Surabaya 60294, Indonesia
Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Engineering Environmental Science Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Public Health Social Sciences Other

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Fostering Biodiversity: Unleashing the Potential of Refugia to Enhance Arthropod Diversity in Chili (Capsicum annuum L.) Farms Reksiana, Catur Putri Enggit; Windriyanti, Wiwin; Kusuma, Ramadhani Mahendra
BIOEDUSCIENCE Vol 7 No 3 (2023): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/12350

Abstract

Background: The utilization of Integrated Pest Management (IPM) offers a solution to chili cultivation pest challenges by implementing ecological engineering strategies, such as planting refugia. These refugia serve as SNAP (Shelter, Nectar, Alternative Food, and Pollen) sources for beneficial insects. This study aimed to assess the impact of refugia planting on arthropod-type diversity and explore the influence of abiotic factors on arthropod populations. Methods: Conducted from February to May 2023, the research utilized various traps (sweep net, yellow trap, pitfall trap, and light trap) and visual control for specimen collection. Results: The identification revealed the presence of three classes, ten orders, and 31 families. Arthropod populations in chilli fields with refugia totaled 867 individuals, whereas those without refugia amounted to 475. Conclusions: Planting refugia led to an elevated diversity index and evenness of arthropods, coupled with a lower dominance index compared to fields without refugia. Surprisingly, temperature and air humidity did not exhibit a significant impact on the arthropod population in chilli fields.
The Increase of Insect Pest Population in Paddy Field Managed with an Ecological Approach using Refugia Plants and Biopesticides Alprilia, Arieska Wahyu; Windiyanti, Wiwin; Kusuma, Ramadhani Mahendra
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 8 No. 1 (2024): June 2024
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.8.1.31-41.2024

Abstract

Insect pests can damage plants and act as vectors of diseases such as brown planthoppers which can transmit dwarf virus disease. The presence of insect pests is influenced by the type of variety, planting patterns, and excessive use of chemical pesticides so that insect pests increase and are difficult to control. This research wanted to provide insights into the effectiveness of combining insectary plants and biopesticides as ecological strategies to manage paddy (Oryza sativa Linnaeus) insect pests. The study was conducted in February-April 2024 in Sukodadi Village, Sukodadi District, Lamongan Regency. Method of study was conducted using a combination of direct observation, insect traps, and quantitative analysis to evaluate the impact of refugia plants and biopesticides on insect pest populations in paddy fields. The analysis includes the species diversity index (Shannon-Winner), species evenness index, species richness index (Margalef), dominance index (Simpson) and similarity index (Bray-Curtis). The study obtained 2,687 individual insect pests in paddy fields with refugia and fobio and 1,761 individuals in paddy fields without refugia and fobio. Insect pests were more abundant in paddy fields with refugia and fobio compared to fields without refugia and fobio. Refugia provides alternative habitats and additional resources for insect pests, and the use of biopesticides can create temporary ecosystem imbalances, and increase the population of insect pest.
Biopesticides and Refugia Implementation Increasing the Population and Diversity of Natural Enemies in Rice Fields based on Ecology Kurniawan, Muhammad Izza; Kusuma, Ramadhani Mahendra; Wiyatiningsih, Sri
Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering) Vol. 14 No. 6 (2025): December 2025
Publisher : The University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jtepl.v14i6.2250-2261

Abstract

The diversity of natural enemies of insects is influenced by the availability of microhabitats and food sources, which can be increased through the use of refugia. Intensive use of pesticides in conventional agricultural systems has resulted in a decline in the diversity of natural enemies that play an important role in biological control of pests. This study aims to identify the types, roles, and populations of natural enemies of pests and determine the level of natural enemy diversity including the species diversity index, species evenness, species richness, and dominance in rice fields using integrated and conventional farming systems. Data collection methods were carried out through a combination of direct observation, insect nets, yellow traps, light traps, and identification of insect morphospecies using a digital microscope and supporting literature. The identification results showed 7 orders and 21 genera of natural enemies with a total of 4,679 individuals in integrated fields and 2,792 individuals in conventional fields. The species diversity index (H’=2.11), evenness (E’=0.69), species richness (R’=2.37), and dominance (C’=0.18) indicated that the integrated system was more supportive of biodiversity. Ecological engineering through refugia has been shown to increase the population diversity of natural enemies, thereby strengthening biological control in rice ecosystems.
Spatial Characterization of NDVI-Based Vegetation Density in Smallholder Coffee Plantation on Mount Kawi’s Southern Slopes Sholikah, Dinna Hadi; Maharani, Nabilla Putry; Kusuma, Ramadhani Mahendra; Bella, Dewi Shasa; Sembiring, Yoga Gregorius; Wijayanti, Fitri; Soemarno, Soemarno
Journal of Applied Agricultural Science and Technology Vol. 9 No. 4 (2025): Journal of Applied Agricultural Science and Technology
Publisher : Green Engineering Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55043/jaast.v9i4.494

Abstract

Indonesia’s coffee cultivation covers 1.25 million hectares, predominantly managed by smallholders (98.14%). Malang Regency, a key production area in East Java, experienced a sharp yield decline from 29,728 tonnes (2021) to 14,151 tonnes (2022). This study investigates smallholder plantations in the Kletek sub-watershed, emphasising the role of shade vegetation in coffee growth. Shade density critically influences productivity and ecological resilience. To support sustainable management, vegetation cover is assessed via remote sensing using the Normalised Difference Vegetation Index (NDVI), enabling spatial analysis of canopy structure. This research aims to analyse the types of coffee shade trees on smallholder coffee farms. NDVI is used to distinguish differences in land cover, including coffee shade. The study employed a land survey using the grid method with 30 observation points. Spatial analysis involves spectral transformation of Sentinel-2A Harmonised imagery, while statistical analysis uses correlation tests. Smallholder coffee farms in the Kletek Sub-watershed feature shade plants such as lamtoro, mahogany, and banana trees. NDVI values across these plantations ranged from moderate (0.4–0.5) to very high (>0.6) vegetation density, showing a strong correlation with land cover conditions (r = 0.80). This confirms NDVI as an effective remote sensing tool for assessing shade vegetation, significantly influencing coffee productivity and ecological resilience. The findings support NDVI-based monitoring for precision agriculture and adaptive management, with scalable applications in sustainable land-use planning, agroforestry optimisation, and climate-resilient coffee cultivation in regions such as Malang Regency, where production has declined.
EKSPLORASI DAN IDENTIFIKASI NEMATODA ENTOMOPATOGEN (Steinernema spp. dan Heterorhabditis spp.) DI DATARAN TINGGI DLUNDUNG KABUPATEN MOJOKERTO PADA KERAPATAN VEGETASI YANG BERBEDA: EXPLORATION AND IDENTIFICATION OF ENTOMOPATHOGENIC NEMATODES (Steinernema spp. and Heterorhabditis spp.) IN THE DLUNDUNG HIGHLAND OF MOJOKERTO DISTRICT AT DIFFERENT VEGETATION DENSITIES Habibah, Isnaini Ainun; Rahmadhini, Noni; Kusuma, Ramadhani Mahendra
Jurnal HPT (Hama Penyakit Tumbuhan) Vol. 13 No. 4 (2025)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jurnalhpt.2025.013.4.7

Abstract

Nematoda entomopatogen (NEP) merupakan agen pengendali hayati yang efektif dalam mengelola hama pertanian karena mampu membunuh inangnya secara cepat dan ramah lingkungan. Penelitian ini bertujuan untuk mengeksplorasi dan mengidentifikasi nematoda entomopatogen (NEP) di dataran tinggi Dlundung, Trawas, Mojokerto, serta menganalisis hubungan antara kerapatan vegetasi dan kepadatan populasinya. Pengambilan sampel dilakukan pada dua lokasi berbeda ketinggian, yaitu 980 mdpl (vegetasi padat, NDVI 0,55) dan 1020 mdpl (vegetasi jarang, NDVI 0,23), dengan metode soil baiting menggunakan larva Tenebrio molitor sebagai umpan. Hasil isolasi menunjukkan adanya dua genus NEP, yaitu Steinernema spp. dan Heterorhabditis spp., yang dibedakan berdasarkan ciri morfologi dan warna kutikula inang terinfeksi. Populasi NEP lebih tinggi pada vegetasi padat (29,51 JI/mL) dibandingkan vegetasi jarang (15,86 JI/mL), meskipun perbedaan tidak signifikan (p > 0,05). Temuan ini menunjukkan bahwa kedua lokasi memiliki kondisi lingkungan yang sesuai bagi perkembangan NEP dan berpotensi menjadi sumber isolat lokal untuk pengendalian hayati hama.
Microclimate Modification through Water Misting Increase Natural Enemy Insect Richness in a Cocoa Agroecosystem Damayanti, Annisa; Windriyanti, Wiwin; Kusuma, Ramadhani Mahendra
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.17799

Abstract

This study aimed to analyze the influence of abiotic factors on the diversity of natural enemies in cocoa fields. The study was conducted from January to March 2025. Various traps were used, including yellow traps, light traps, pitfall traps, and visual observations. Identification showed that cocoa fields with misting applications had a higher number of natural enemies (1,052 individuals) compared without misting fields (658 individuals). The application of misting resulted in higher diversity indices (H'), richness (R'), and dominance (C'), as well as lower evenness (E') compared to untreated fields. These findings suggest that the misting system creates a more favorable microhabitat for natural enemy diversity, thus potentially strengthening the IPM approach in cocoa cultivation. Interestingly, the analysis showed that temperature had a significance value (sig) > 0.05, indicating that temperature did not show a significant correlation with the increase in the abundance of natural enemies, while humidity has a significance value (sig) < 0.05, which shows that humidity has a significant correlation, although statistically relatively small, with the increase in the abundance of natural enemies.
Karakterisasi Senyawa Pestisida pada Nanopartikel Ekstrak Bawang Putih (Allium Sativum L.) dan Uji Mortalitas Terhadap Ulat Grayak (Spodoptera Litura F.) di Laboratorium Josua, Wahyu Ramadhani Putra; Windriyanti, Wiwin; Kusuma, Ramadhani Mahendra
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 4 (2025): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i4.18441

Abstract

This study aims to characterize the pesticide compounds in garlic (Allium sativum L.) extract nanoparticles and test their mortality effectiveness against armyworms (Spodoptera litura F.) in the laboratory. Nanoparticle synthesis was carried out using the ionic gelation method with chitosan-TPP, which was subsequently characterized using PSA, SEM, FT-IR, and GC-MS. The characterization results showed a particle size of 930.52 nm with a homogeneous distribution (PDI 0.147). GC-MS analysis successfully identified the main bioactive organosulfur compounds, namely diallyl disulfide (20.72%), diallyl trisulfide (14.16%), and diallyl tetrasulfide (5.79%). Bioactivity testing using the sandwich method at five concentration variations (0-1%) showed the highest mortality reaching 70% at a 1% concentration. Probit analysis yielded values of LC₅₀ 1.116% and LC₉₀ 1.720%, as well as LT₅₀ 5.117 days and LT₉₀ 9.893 days. Statistical test results indicated a significant difference (p<0.05) between all treatments and the control group. Thus, it can be concluded that the garlic extract nanoparticle formulation has the potential to be an effective nano-bioinsecticide, with a mode of action that is dose-dependent and time-dependent in controlling S. litura larvae.
Production and Characterization of Nanoparticles from Citronella (Cymbopogon nardus) Extract Based on Chitosan Compounds Damayanti, Alfa Dirsista; Rahmadhini, Noni; Kusuma, Ramadhani Mahendra
BIOEDUSCIENCE Vol 9 No 3 (2025): BIOEDUSCIENCE
Publisher : Universitas Muhammadiyah Prof. Dr. Hamka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22236/jbes/18769

Abstract

Background: The use of nanoparticles in Indonesia has been developing and is now being applied across sectors, including agriculture, health, and industry. Nanoparticles derived from Cymbopogon nardus can be used as nanopesticides because they contain several compounds that suppress pathogen growth and reduce pest populations. Chitosan is an additional compound used in the fabrication of these nanoparticles. Methods: This research used materials and tools, including a PSA (Particle Size Analyzer), a SEM (Scanning Electron Microscope), Cymbopogon nardus, and chitosan. The research stages included extraction of Cymbopogon nardus, nanoparticle fabrication, PSA analysis, zeta potential measurement, and SEM observation. Results: The PSA test revealed that the Cymbopogon nardus nanoparticles had an average size of 907.2 nm. The zeta potential of the nanoparticles was –40.62 mV, indicating good electrostatic stability. SEM images showed that the nanoparticles had an irregular shape. Conclusions: The Cymbopogon nardus nanoparticles produced in this study, as characterized by PSA, zeta potential, and SEM analyses, demonstrated the expected characteristics of nanoparticles.
Potensi Jamur Beauveria Bassiana (Balsamo) Vuillemin (Hypocreales: Cordycipitaceae) Sebagai Jamur Endofit Terhadap Pertumbuhan Kacang Panjang (Vigna sinensis L.) Naufal, Raihan; Kusuma, Ramadhani Mahendra; Purnawati, Arika
Bioscientist : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2026): March
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v14i1.19725

Abstract

This study aimed to evaluate the potential of the entomopathogenic fungus Beauveria bassiana as an endophytic agent through soil drenching application and to assess its effect on the growth of yardlong bean plants (Vigna unguiculata subsp. sesquipedalis). The research was conducted under both in vitro and in vivo conditions using a Completely Randomized Design (CRD) with four conidial density treatments: control (distilled water), 1 × 10⁸, 1 × 10⁷, and 1 × 10⁶ conidia/mL, each replicated five times. Data were analyzed using ANOVA followed by Tukey’s Honest Significant Difference (HSD) test at a 5% significance level. The results demonstrated that B. bassiana successfully colonized plant tissues, with the highest colonization rate observed at 1 × 10⁸ conidia/mL, reaching 83.3% in roots and 66.7% in stems. Although differences in conidial density did not consistently produce significant effects on plant height and leaf number across all observation periods, inoculated treatments tended to show higher average growth values compared to the control, particularly at 28 days after planting. It can be concluded that soil application of B. bassiana is effective in establishing endophytic colonization without inhibiting vegetative growth, indicating its potential as an environmentally friendly pest management strategy.
Co-Authors Aditya, Haidar Fari Agustin, Eva Tri Ahmad Ilham Tanzil Ahmad Ilham Tanzil Ajeng, Karenina Alprilia, Arieska Wahyu Amanda Nur Fitria Ningrum Ankardiansyah Pandu Pradana Anwari, Muhammad Alfian Arika Purnawati Arrohmatus Syafaqoh Li'aini Azahra, Raudhatul Barna Deta Cristin Naftaly Bayu Widhayasa Bella, Dewi Shasa Carolina Rosa Siallagan Catur Putri Enggit Reksiana Chosa Zahro Fatiha Damayanti, Alfa Dirsista Damayanti, Annisa Deviyanti, Nur Qomariyah Didik Utomo Pribadi Dinna Hadi Sholikah Dita Megasari Dwi Wahyuningtyas Fachmi, Anivea Fitri Wijayanti Fitri Wijayanti Fitriyani, Novi Nur Habibah, Isnaini Ainun Haidar Fari Aditya Hamidah . Haryo Sulistiyantoro Ira Kaestiningtyas Irwanto Sucipto Jaka Wijaya Kusuma Josua, Wahyu Ramadhani Putra Juwito Juwito Kurniawan, Muhammad Izza Kusuma Wardhani Mas’udah Maharani, Nabilla Putry Mandasari, Virginia Mitha Anggisna Angreini MOCH SODIQ, MOCH Mochamad Arifin Mohamad Hipti Mukhlish Jamal Musa Holle Naufal, Raihan Nijma Nurfadila Noni Rahmadhini Prahasti Ken Dewani Pratama, Kevin Dimas Puji Lestari Tarigan Purnomo Edi Sasongko Putri, Firda Amalia Reksiana, Catur Putri Enggit Restu Nugraha Rohmalia, Fitri Roziana Febrianita Safeyah, Muchlisiniyati Safira Rizka Lestari Sasongko, Edi Purnomo Sembiring, Yoga Gregorius Septiandari, Renata Soemarno Soemarno Soleudin Efendi Sri Wiyatiningsih Suharto Sutini Torino Benarivo Tri MUJOKO Tria Puspa Sari Trijanti A. Widinni Asnan Wanti Mindari Widajati, Wiludjeng Wiga Fikri Destianisma Wiludjeng Widajati Wiludjeng Widajati Windiyanti, Wiwin Windriyanti, Wiwin Windriyati, Wiwin Wiwin Windriyanti Wiwin Windriyanti Yosua Nathanael Itona Sagala YUDI NUR SUPRIADI Yuliawati, Ira Sukma Zahro, Balqis Ghaitza Zainal Abidin Achmad