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All Journal Journal of Tropical Soils Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Berkala Ilmu Kesehatan Kulit dan Kelamin Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) The Indonesian Biomedical Journal Sari Pediatri Jurnal Ekonomi Manajemen JIIP (Jurnal Ilmiah Ilmu Pendidikan) Abdi Laksana : Jurnal Pengabdian Kepada Masyarakat International Journal of Social and Management Studies (IJOSMAS) International Journal on Advanced Technology, Engineering, and Information System (IJATEIS) Jurnal Peradaban Masyarakat (JPM) Journal Research of Social Science, Economics, and Management Jurnal PERKUSI: Pemasaran, Keuangan dan Sumber Daya Manusia Indonesian Journal of Biomedicine and Clinical Sciences Cakrawala: Jurnal Ekonomi, Manajemen dan Bisnis International Journal of Social Science and Community Service INFOKOM Folia Medica Indonesiana
Arief Budiyanto
Department Of Dermatovenerology, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada, Yogyakarta
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Other

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The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2-Deoxyguanosine Formation in Human Skin Arief Budiyanto, Irianiwati, Catharina Sagita Yohanes Widodo Wirohadidjojo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 02 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Ultraviolet B (UVBI may cause DNA damage due to photooxydative reaction and form 8hydroxy-2-deoxyguanosine (8-0HdGI as a genotoxic product. Topically extra virgin olive oil (EVOOI can decrease 8-0HdG formation among UVB irradiated-hairless mice epidermal cells. Such effect in human skin has never been discovered.Objective: To know the effect of topically EVOO in UVB induced-8-0HdG formation in human epidermal cells.Method: A simple experiment was performed on cultured human foreskins. The foreskins were divided into 3 groups: group was treated with 100mJ/cm2 UVB + topical EVOO, group B with 100mJ/cm2 UVB, and group C as control. All skin was cultured in completed DMEM and fixation was performed at one and 24 hours. 8-0HdG expression was determined immunohistochemically with antibody anti 8-0HdG and measured based on histogram of selected colour of Adobe photoshop.Results: The mean of 8-0HdG expression in experimental groups: (8.02:t 13.81, (26.95:t 23.231. and 14.96:t 7.141, respectively (p =0.0001 at an hour; (14.91 :t30.63I, (37.58:t 25.631. and (8.76:t 10.941 respectively (p = 0.0001 at 24 hours.Conclusion: Extra virgin olive oil was proved to reduce the 8-0HdG formation in exposed human skin-UVB. and may be developed to be anti-photocarcinogenesis active ingredient topically.Kata kunci:Extra virgin olive oil, UVB, 8-OHdG, human Skin
Targeted phototherapy for skin diseases Arief Budiyanto, Sa’adatul Huriyah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Universitas Gadjah Mada

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Abstract

Phototherapy is a therapeutic strategy in dermatology for treating several skin diseases.Conventional phototherapy has some disadvantages, hence a targeted phototherapy that emitsselective laser or ultraviolet (UV) light and targets specific area of affacted skin has been developed.Targeted phototherapy is considered to be more aggressive and has higher efficacy. Severaltargeted phototherapy devices emit one or several types of light. The use of targeted phototherapyhas been studied in vitiligo, psoriasis, eczema, keloid, hypopigmented scar or striae alba, andoral lichen planus.
The effect of topically extravirgin olive oil on the UVB-induced immunosuppression AriefBudiyanto, Irianiwati, Vohanes Widodo Wirohadidjojo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 03 (2009)
Publisher : Universitas Gadjah Mada

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Abstract

Background: UVBradiation may act as an immunosuppressive agent through Langernans cells (LCs)depletion correlated with cyclobuthane pyrimidine dimer (CPD), as the most mutagenic photoproducts. Other studies showed that olive oil can prevent various human cancers, which are defect of immune-surveillance. The effect of olive oil in the UVBinduced LCs depletion is still unclear. Objective: To discover the topical effect of extravirgin-olive-oil in the LCs depletion. Methods: A simple experimental study was performed on foreskins collected from 8 circumcised boys. Each of them was cut into 3 pieces of 0.5 cm2, grouped randomly to: (Olive oil+ UVB)group, (placebo + UVB)group, and unirradiated group. They were cultured in DMEM for 24 hours, irradiated with 100mJs/cm2 UVB, and olive oil or placebo were then applied immediately after irradiation. All specimens were fixed in buffered-formalin, paraffin blocked, and cut to be 2 slides, and each of them was stained with anti-CD 1a or anti-CPD antibody. The pixels of AEC signats were digitally counted based on Photoshops selected color histogram.The pixel means of various groups were analyzed by Kruskal-Wallis test and post hoc analysis by Mann Whitney test. Results: Olive oil treated groups showed a very significant (P
Pure Neural Leprosy Verdy, Verdy; Budiyanto, Arief; Siswati, Agnes Sri
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 27, No 1 (2015): BIKKK APRIL 2015
Publisher : Faculty Of Medicine Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (285.927 KB) | DOI: 10.20473/bikkk.V27.1.2015.70-76

Abstract

Background: Pure neural leprosy (PNL) is an infection by Mycobacterium leprae with clinical manifestation as nerve thickening, sensory nerve impairment, with or without motoric nerve involvement, and without skin lesion. Pure neural leprosy causes progressive degeneration of nerve function. Diagnosis establishement of PNL is difficult. Purpose: To discuss about PNL especifically in aspects of the diagnosis and therapy. Reviews: Gold standard in diagnosis of PNL is histopathological examination from nerve biopsy but this procedure is still difficult for various reasons. Some investigations can be done for PNL such as cytological fine needle aspiration, Mitsuda test, serology test for antibody anti-PGL1, electroneuromyography (ENMG), and polymerase chain reaction (PCR). Pure neural leprosy classification is based on findings of abnormality in neurological, immunological, and histopathological. Pure neural leprosy therapy is based on the classification of paucibacillary or multibacillary. Conclusions: The clinical and epidemiological findings plus with the electroneuromyography alteration were finally accepted as sufficient criteria to diagnose PNL. Pure neural leprosy therapy follows WHO regiment in accordance with the paucibacillary or multibacillary classification.Key words: pure neural leprosy, Mycobacterium leprae, nerve biopsy, anti-PGL-1, electroneuromyography.
Successful Treatment of Actinomycetoma with Combination of Cotrimoxazole and Tetracycline Verdy, Verdy; Dewi, Vina Ajeng Puspa; Budiyanto, Arief; Siswati, Agnes Sri
Berkala Ilmu Kesehatan Kulit dan Kelamin Vol 27, No 1 (2015): BIKKK APRIL 2015
Publisher : Faculty Of Medicine Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1047.729 KB) | DOI: 10.20473/bikkk.V27.1.2015.77-83

Abstract

Background: Actinomycetoma is an infection of chronic granulomatous disease on the skin, subcutaneous tissue, fascia, and bone, occured after trauma because of bacteria innoculation. Clinical features seem painless nodules, abscess, fistula, and sinus  with granulated discharge.Gold standard treatment for actinomycetoma is cotrimoxazole. Combination therapy with other antibiotic is needed to prevent resistance and improve therapeutic efficacy. Purpose: To discuss successful treatment of actinomycetoma with combination of cotrimoxazole and tetracycline Case: A 19-years-old woman had complained of  chronic post-traumatic wounds since two years ago. Dermatological examination showed multiple painless nodules, sinus, and discharge on right dorsum pedis. Histopathological examination demonstrated sinus in dermis, suppurative granulomas, and SplendoreHoeppli phenomenon in dermis layer. Case Management: Combination therapy consist of cotrimoxazole 2x960 mg, that was given for 6 months and tetracycline 4x500 mg, that was given in the first 1 month.  No side effects and drug allergy during and/or post treatment was found. After clinical evaluation for 6 months, successful of treatment  was obtained and no recurrence was found. Conclusion: The combination therapy of cotrimoxazole for 6 months and tetracycline for 1 month was proven to be effective and no recurrence was found.Key words: actinomycetoma, cotrimoxazole, tetracycline.
Pathogenesis, evaluation, and recent management of diabetic foot ulcer Wijaya, Lorettha; Budiyanto, Arief; Astuti, Indwiani; Mustofa, .
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 1 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.122 KB) | DOI: 10.19106/JMedSci005101201910

Abstract

Diabetic foot ulcers (DFUs) are a major health problem as the number of patients continues to increase, are difficult to heal, require enormous management costs, and deteriorate the quality of life of patients, their families and societies. The pathogenesis of DFUs is complex. Most important factors that increase the risk of DFUs are peripheral neuropathy, foot deformities, frequent minor trauma, and peripheral arterial disease. Neuropeptides derangement, hypoxia, hyperglycemia, and infection act as the cause of chronicity of DFUs. Therefore, during the initial evaluation of DFU, patients need to be checked for their metabolic status, presence or absence of peripheral neuropathy, vascular insufficiency, foot deformities, and infection of the ulcer and its underlying bone. Then, DFUs are classified by the severity of vascular insufficiency, the depth of the wound, and the severity of the infection. This classification system helps clinicians to determine whether the patient needs to be hospitalized or amputated and helps to establish DFU management strategies. In the management of DFUs, adequate blood flow to the wound area should be achieved. Glycemic control and standard wound care should be encouraged. Standard wound care includes debridement, offloading, wound moisture balance with suitable dressing, edema control, and infection control. Education about preventive foot care should be taught to the patients and their families. As the pathogenesis and management of DFUs are complex, a multidisciplinary team consists of expert individuals in their respective fields should be involved.
The effect povidone-iodine on the wound healing process: A study on fibroblast populated collagen lattice (FPCL) model Retno Danarti; . Suswardana; Arief Budiyanto; Widodo Wirohadidjojo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 46, No 03 (2014)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (113.34 KB) | DOI: 10.19106/JMedScie.004603201401

Abstract

Povidone-iodine (PI) 10% solution is an effective antiseptic. However, it appears to be toxic tothe cells involved in wound healing. The aim of this study is to evaluate the toxicity of PI oncultured human fibroblast using fibroblast populated collagen lattice (FPCL) model. The culturedhuman fibroblast was divided into 6 groups i.e. 5 groups were exposed by PI 1, 0.1, 0.01, 0.001and 0.0001%, and 1 group was exposed by phosphate-buffered-saline (PBS). Twenty-four hourslater, the media was washed using PBS. The size of the FPCL media on each group wasobserved over time by serial photographs, which then were measured by Image-J computerprogram. Exposure of 0.1, 0.01, 0.001 and 0.0001 PI caused an obvious reduction of fibroblast’scontraction capability on FPCL media, which described temporary fibroblast injury, that showinga concentration-dependent recovery phenomenon after 48th hour. Furthermore, 1% PI exposureleads to a permanent fibroblast injury. In conclusion, PI exposure in concentration more than0.1% has a permanent toxic effect on fibroblast that clearly observed using a simple FPCLmodel.
The Effect of PRF on Serum Starved Human Dermal Fibroblast. Sunardi Radiono; Yohanes Widodo Wirohadidjojo; Arief Budiyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 2 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (461.425 KB) | DOI: 10.19106/JMedSci004802201605

Abstract

ABSTRACTHealing failure on chronic ulcers was suspected due to the decrease of Growth Factors (GFs) supply caused by either GFs trapped in the fibrin, or degraded by protease, or decreased level due to reduction of GFs gene expression. Administration of various GFs can stimulate healing of chronic ulcers. High level of GFs is available in biologic material called Platelet Rich Fibrin (PRF). This study was conducted to have in vitro evidence of PRF effect on GFs-serum starved human dermal fibroblasts as representative cells of chronic ulcers. Human dermal fibroblasts (HDFs) were isolated from foreskin of six boys aged 11- 14 years-old. After 24 hours of serum deprivation, HDFs were treated by 100, 50, and 25% PRF lysate diluted in cultured medium. Cellular migration was measured using scratch assay, while cellular viability was measured using MTT assay and collagen deposition was measured using Sirius Red assay. The HDFs of serum starvation group showed significant impairment activities in terms of cellular migration (25%), cellular proliferation (20%), and collagen deposition (10%) (p<0.05). Administration with various levels of PRF lysate could significantly recover those activities (p < 0.05). Because cellular activities of serum starved HDFs is similar with fibroblasts isolated from the bottom of chronic ulcers and administration of various levels of PRF lysate was capable to recover those activities, it can be concluded that PRF is a good biologic material to stimulate healing of chronic ulcers. However, in order to get better evidence based medicine, both pre clinical and clinical studies must be performed.
Effect of tagitinin C isolated from Tithonia diversifoli (Hemsley) A Gray on migration activity and TGF-β1 levels on keloid fibroblast Elvira Santi; Mae Sri Hartati Wahyuningsih; Arief Budiyanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 51, No 3 (2019)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (235.706 KB) | DOI: 10.19106/JMedSci005103201901

Abstract

Keloid is the formation of excessive scar tissue characterized by fibroblast hiperproliferations and collagen deposits that are similar with cancer cells. Tagitinin C is proven can inhibit proliferation and deposition of keloids collagen fibroblast. However, the mechanism of action of tagitinin C in migration activities and TGF-β1 levels of keloid fibroblasts has not been proved, yet. This study aimed to investigate the effects of tagitinin C isolated from Tithonia diversifoli (Hemsley) on migration activity and TGF-β1 expression of keloid fibroblast. This was quasi experimental study with post test only controlled group design using keloid fibroblasts isolated from keloid patients. The migration activity were performed by scratch assay and TGF-β1 levels were measured using an ELISA kits. Isolate tagitinin C was more active inhibit fibroblast keloid migration compare to the control groups (p<0.05) after 48 h incubation. TGF-β1 levels after incubation with isolate tagitinin C was lower then control group (p<0.05). In conclusion, isolate tagitinin C can inhibit migration and reduce TGF-β1 levels on keloid fibroblast
hsa-miR-376c-3p in The Circulating Plasma is Upregulated in The Elderly Javanese Male When Compared to Their Younger Counterparts Ana Lucia Ekowati; Zacharias Aloysius Dwi Pramono; Daniel Ardian Soeselo; Arief Budiyanto; Indwiani Astuti; Sofia Mubarika
The Indonesian Biomedical Journal Vol 11, No 3 (2019)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v11i3.827

Abstract

BACKGROUND: MicroRNA (miRNA), short noncoding RNA, plays role in various physiological process such as aging through epigenetic regulation of gene expression. miRNA present intracellular as well as extracellular in body fluids. miRNA that present in blood circulatory system is often referred as circulatory miRNA (c-miRNA). A number of studies trying to identify c-miRNAs as biomarker for ageing have been reported, but majority did not yield results that corroborate one with another. This study reports the identification of a differentially expressed c-miRNAs between elderly and youth groups of individuals, the first step in tracking specific miRNAs that play role in physiologic ageing.METHODS: The miRNA expression profiles of grandfathers and grandsons from 2 Javanese families were compared to select 5 miRNA candidates with widest expression difference. The 5 candidates were subjected to validation using quantitative polymerase chain reaction (qPCR) in 11 elderly men and 9 young men of the same ethnicity to identify differentially expressed miRNA between elder and younger male groups in the represented population.RESULTS: Amongst 5 selected c-miRNA candidates, the hsa-miR-376c-3p was validated to be upregulated in the elderly group when compared to the young individuals. Bioinformatic analysis using miRTarBase 7.0, miRTargetLink Human and GeneCards® Human Gene Database suggest the involvement of hsa-miR-376c-3p in pathways relevant with cellular ageing.CONCLUSION: This study showed that hsa-miR-376c-3p in the circulating plasma to be significantly upregulated in a group of elderly Javanese males compared to their younger counterparts. The results of this study warrant further study to elucidate the specific role of hsa-miR-376c-3p in physiologic ageing mechanism.KEYWORDS: circulating, microRNA, miR-376c-3p, elderly, Javanese, male
Co-Authors . Suswardana ACHMAD RACHMAN Agnes Sri Siswati Alchusnah, Retno Hayati Alfieri, Alessandro Ana Lucia Ekowati Ariani, Ratri Asep Erlan Maulana Cahyo, Rahmat Nur Chandra Wahyu Purnomo Daniel Ardian Soeselo Ekaputra, Ericko Elvira Santi Eviani, Fortunia Mona Fajar Waskito Febiyanto, Novian Hanan Asrafi Noviandari Hardyanto Soebono Ika Puspitasari Imbron, Imbron Indwiani Astuti Indwiani Astuti Komala, Laura Lorettha Wijaya Lukman Ariwibowo Mae Sri Hartati Wahyuningsih Marleno, Risma Masádi , Mahnun Mas’adi, Mahnun Meidiyanti, Prima Mira Oktarina, Dyah Ayu Muchamad Apriyanto Muhammadun, Haris Mustofa Mustofa Niken Indrastuti Niken Indrastuti Niken Trisnowati Nugroho, Oka Nurhadi, Ahmad Pamungkas, Ibrahim Bali Praditya, Alvin Rodiyana, Nanda Sarastuti, Dewi Sari, Ade Riska Satiti Retno Pudjiati Satiti Retno Pudjiati Sholeh, Achmad Nur Sigid Santosa, Novita Permatasari Sofia Mubarika Stella, Maureen Miracle Suwito, Darwito Tajuddien, Rahadyan Verdy Verdy Verdy Verdy, Verdy Vina Ajeng Puspa Dewi Vina Ajeng Puspa Dewi, Vina Ajeng Puspa Wibowo, Wahyu Andri Yohanes Widodo Wirohadidjojo Yusmiyati, Yusmiyati Zacharias Aloysius Dwi Pramono zaman, M. Badru